Tackling exosome and nuclear receptor interaction: an emerging paradigm in the treatment of chronic diseases DOI Creative Commons

Babu Santha Aswani,

Mangala Hegde, Ravichandran Vishwa

et al.

Military Medical Research, Journal Year: 2024, Volume and Issue: 11(1)

Published: Sept. 26, 2024

Abstract Nuclear receptors (NRs) function as crucial transcription factors in orchestrating essential functions within the realms of development, host defense, and homeostasis body. NRs have garnered increased attention due to their potential therapeutic targets, with drugs directed at demonstrating significant efficacy impeding chronic disease progression. Consequently, these pharmacological agents hold promise for treatment management various diseases. Accumulating evidence emphasizes regulatory role exosome-derived microRNAs (miRNAs) inflammation, progression, therapy resistance, primarily by modulating factors, particularly NRs. By exploiting inflammatory pathways such protein kinase B (Akt)/mammalian target rapamycin (mTOR), nuclear factor kappa-B (NF-κB), signal transducer activator 3 (STAT3), Wnt/β-catenin signaling, exosomes play a pivotal panorama physiology, pathology. The internalization modulates initiates diverse autocrine or paracrine signaling cascades, influencing processes recipient cells survival, proliferation, differentiation, metabolism, cellular defense mechanisms. This comprehensive review meticulously examines involvement exosome-mediated NR regulation pathogenesis ailments, including atherosclerosis, cancer, diabetes, liver diseases, respiratory conditions. Additionally, it elucidates molecular intricacies communication between via NRs, leading immunomodulation. Furthermore, outlines implications exosome-modulated prophylaxis delineates current limitations, provides insights into future perspectives. also presents existing on components emergence resistance.

Language: Английский

Programming assembly of biomimetic exosomes: An emerging theranostic nanomedicine platform DOI Creative Commons
Xiao Xu, Limei Xu, Caining Wen

et al.

Materials Today Bio, Journal Year: 2023, Volume and Issue: 22, P. 100760 - 100760

Published: Aug. 11, 2023

Exosomes have emerged as a promising cell-free therapeutic approach. However, challenges in large-scale production, quality control, and heterogeneity must be overcome before they can used clinically. Biomimetic exosomes containing key components of natural been assembled through extrusion, artificial synthesis, liposome fusion to address these limitations. These exosome-mimetics (EMs) possess similar morphology function but provide higher yields, faster size compared conventional exosomes. This article provides an overview the chemical biological properties various synthetic exosome systems, including nanovesicles (NVs), EMs, hybrid We highlight recent advances production applications nanobiotechnology discuss advantages, limitations, potential clinical programming assembly mimetics.

Language: Английский

Citations

51
Graphene Sensor Arrays for Rapid and Accurate Detection of Pancreatic Cancer Exosomes in Patients’ Blood Plasma Samples DOI Creative Commons
Tianyi Yin, Lizhou Xu, Bruno M. G. Rosa

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(15), P. 14619 - 14631

Published: July 20, 2023

Biosensors based on graphene field effect transistors (GFETs) have the potential to enable development of point-of-care diagnostic tools for early stage disease detection. However, issues with reproducibility and manufacturing yields sensors, but also Debye screening unwanted detection nonspecific species, prevented wider clinical use technology. Here, we demonstrate that our wafer-scalable GFETs array platform enables meaningful results. As a case study high relevance, an accurate robust portable GFET biosensor pancreatic ductal adenocarcinoma (PDAC) in patients' plasma through specific exosomes (GPC-1 expression) within 45 min. In order facilitate reproducible blood plasma, optimized analytical performance biosensors via application internal control channel test protocol. Based samples from 18 PDAC patients 8 healthy controls, arrays could accurately discriminate between two groups while being able detect cancer stages including 1 2. Furthermore, confirmed higher expression GPC-1 found concentration was average more than magnitude samples. We these characteristics cancerous are responsible increase number target surface graphene, leading improved signal response biosensors. This holds great promise tool rapid diagnosis cancer.

Language: Английский

Citations

43
Tumor-derived small extracellular vesicles in cancer invasion and metastasis: molecular mechanisms, and clinical significance DOI Creative Commons
Chi Zhang, Chaoying Qin, Saikat Dewanjee

et al.

Molecular Cancer, Journal Year: 2024, Volume and Issue: 23(1)

Published: Jan. 19, 2024

Abstract The production and release of tumor-derived small extracellular vesicles (TDSEVs) from cancerous cells play a pivotal role in the propagation cancer, through genetic biological communication with healthy cells. TDSEVs are known to orchestrate invasion-metastasis cascade via diverse pathways. Regulation early metastasis processes, pre-metastatic niche formation, immune system regulation, angiogenesis initiation, matrix (ECM) remodeling, modulation, epithelial-mesenchymal transition (EMT) among pathways regulated by TDSEVs. MicroRNAs (miRs) carried within as double-edged sword can either promote or inhibit cancer progression. serve excellent markers for detection tumors, tumor metastases. From therapeutic point view, risk may be reduced limiting On other hand, represent promising approach vivo delivery cargo present review article discusses recent developments current views field research clinical applications.

Language: Английский

Citations

35
Extracellular Vesicle Preparation and Analysis: A State‐of‐the‐Art Review DOI Creative Commons
Zesheng Wang, Xiaoyu Zhou, Qinglong Kong

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(30)

Published: June 14, 2024

Abstract In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the life sciences due to their critical roles both health and disease states, offering promising applications diagnosis, drug delivery, therapy. However, inherent heterogeneity complex origins pose significant challenges preparation, analysis, subsequent clinical application. This review is structured provide an overview of biogenesis, composition, various sources EVs, thereby laying groundwork for a detailed discussion contemporary techniques preparation analysis. Particular focus given state‐of‐the‐art technologies that employ microfluidic non‐microfluidic platforms EV processing. Furthermore, this discourse extends into innovative approaches incorporate artificial intelligence cutting‐edge electrochemical sensors, with particular emphasis single proposes current outlines prospective avenues future research. The objective motivate researchers innovate expand methods analysis fully unlocking biomedical potential.

Language: Английский

Citations

35
Clinical Impact of Multi-omics profiling of extracellular vesicles in cancer Liquid Biopsy DOI Creative Commons
Mrunal Kulkarni, Rishav Kar,

Srestha Ghosh

et al.

The Journal of Liquid Biopsy, Journal Year: 2024, Volume and Issue: 3, P. 100138 - 100138

Published: Jan. 4, 2024

Extracellular vesicles (EVs) are the cell's secreted component. It is majorly classified into microvesicles, apoptotic bodies, and exosomes. Exosomes play a significant role in cancer development progression. Its molecular signature (DNA, RNA, Proteins, lipids) has more priority profiling current decade. In prevention, most challenging part early detection. EVs-based screening develops promising platform. Multi-Omics exosomes profiling-based liquid biopsy support detection efficient way. This approach provides detailed expression data (it may be inner cargos or surface express molecules). article highlited multi-omic exosome theranostics applications cancer, technical challenges, improvisation for future improvement.

Language: Английский

Citations

16
Mesenchymal stem cells derived exosomes: a new era in cardiac regeneration DOI Creative Commons
Hossein Rayat Pisheh, Mahsa Sani

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 23, 2025

Language: Английский

Citations

3
Research Advances of Engineered Exosomes as Drug Delivery Carrier DOI Creative Commons
Lianghui Huang,

Enguang Wu,

Jiawei Liao

et al.

ACS Omega, Journal Year: 2023, Volume and Issue: 8(46), P. 43374 - 43387

Published: Nov. 9, 2023

Exosomes are nanoscale vesicles secreted by living cells that have similar membrane composition to parental and carry a variety of proteins, lipids, nucleic acids. Therefore, exosomes certain biological activities play an important role in intercellular communication. On the basis its potential as carrier for drug delivery systems, been engineered compensate shortage natural through various engineering strategies improving efficiency, enhancing targeting tissues organs, extending circulating half-life exosomes. This review focuses on loading drugs different strategies, discussions exosome surface modification summarizes advantages disadvantages strategies. In addition, this provides overview recent applications number refractory relapsable diseases. has provide reference further research development

Language: Английский

Citations

35
The Regulation of Exosome Generation and Function in Physiological and Pathological Processes DOI Open Access
Ying Wang,

Tong Xiao,

Chaoran Zhao

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 25(1), P. 255 - 255

Published: Dec. 23, 2023

Exosomes, a type of extracellular vesicle with diameter approximately 100 nm that is secreted by all cells, regulate the phenotype and function recipient cells carrying molecules such as proteins, nucleic acids, lipids are important mediators intercellular communication. Exosomes involved in various physiological pathological processes immunomodulation, angiogenesis, tumorigenesis, metastasis, chemoresistance. Due to their excellent properties, exosomes have shown potential application clinical diagnosis treatment disease. The functions depend on biogenesis, uptake, composition. Thus, deeper understanding these regulatory mechanisms can help find new targets for disease therapy. Therefore, this review summarizes integrates recent advances entire biological process exosomes, starting from formation early-sorting endosomes (ESCs) plasma membrane invagination release fusion multivesicular bodies (MVBs) membrane, well interactions between cells. We also describe discuss exosome production tumor used cancer

Language: Английский

Citations

24
The Advances and Applications of Characterization Technique for Exosomes: From Dynamic Light Scattering to Super-Resolution Imaging Technology DOI Creative Commons
Shijia Wu,

Yalan Zhao,

Zitong Zhang

et al.

Photonics, Journal Year: 2024, Volume and Issue: 11(2), P. 101 - 101

Published: Jan. 23, 2024

Exosomes distributed by extracellular vesicles carry various information highly consistent with cells, becoming a new type of biomarker for tumor screening. However, although conventional characterization technologies can quantify size and morphology exosomes, they are limited in related fields such as function tracing, protein quantification at unit point, microstructural information. In this paper, firstly, different exosome methods systematically reviewed, dynamic light scattering, nanoparticle tracking analysis, flow cytometry, electron microscope, emerging super-resolution imaging technologies. Then, advances applications described one one. Last but not least, we compare the features exosomes propose that technology only take into account advantages techniques also provide accurate, real-time, quantitative analysis exosomes. It provides fine guide exosome-related biomedical research, well application liquid biopsy techniques.

Language: Английский

Citations

13
Exosome-derived circUPF2 enhances resistance to targeted therapy by redeploying ferroptosis sensitivity in hepatocellular carcinoma DOI Creative Commons

Fenglin Dong,

Zongzhen Xu,

Ying-Qiao Wang

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 30, 2024

Abstract Background Advanced hepatocellular carcinoma (HCC) can be treated with sorafenib, which is the primary choice for targeted therapy. Nevertheless, effectiveness of sorafenib greatly restricted due to resistance. Research has shown that exosomes and circular RNAs play a vital role in cancer’s malignant advancement. However, significance exosomal development resistance HCC remains uncertain. Methods Ultracentrifugation was utilized isolate (Exo-SR) from sorafenib-resistant cells’ culture medium. Transcriptome sequencing differential expression gene analysis were used identify targets Exo-SR action cells. To cells, transcriptome genes employed. evaluate impact circUPF2 on HCC, experiments involving gain-of-function loss-of-function conducted. RNA pull-down assays mass spectrometry performed RNA-binding proteins interacting circUPF2. immunoprecipitation (RIP), pull-down, electrophoretic mobility shift assay (EMSA), immunofluorescence (IF) -fluorescence situ hybridization (FISH), rescue validate interactions among circUPF2, IGF2BP2 SLC7A11. Finally, tumor xenograft examine biological functions underlying mechanisms vivo. Results A novel circRNA, identified revealed significantly enriched Exo-SR. Exosomes enhanced by promoting SLC7A11 suppressing ferroptosis Mechanistically, acts as framework enhance creation circUPF2-IGF2BP2-SLC7A11 ternary complex contributing stabilization mRNA. Consequently, promotes enhances function system Xc- leading decreased sensitivity sorafenib. Conclusions The facilitated formation increases stability Focusing could potentially an innovative approach treatment. Graphical

Language: Английский

Citations

12