Simvastatin/hydrogel-loaded 3D-printed titanium alloy scaffolds suppress osteosarcoma via TF/NOX2-associated ferroptosis while repairing bone defects DOI Creative Commons
Zehao Jing, Wanqiong Yuan, Jiedong Wang

и другие.

Bioactive Materials, Год журнала: 2023, Номер 33, С. 223 - 241

Опубликована: Ноя. 17, 2023

Postoperative anatomical reconstruction and prevention of local recurrence after tumor resection are two vital clinical challenges in osteosarcoma treatment. A three-dimensional (3D)-printed porous Ti6Al4V scaffold (3DTi) is an ideal material for reconstructing critical bone defects with numerous advantages over traditional implants, including a lower elasticity modulus, stronger bone-implant interlock, larger drug-loading space. Simvastatin multitarget drug anti-tumor osteogenic potential; however, its efficiency unsatisfactory when delivered systematically. Here, simvastatin was loaded into 3DTi using thermosensitive poly (lactic-co-glycolic) acid (PLGA)-polyethylene glycol (PEG)-PLGA hydrogel as carrier to exert anti-osteosarcoma effects. Newly constructed simvastatin/hydrogel-loaded (Sim-3DTi) comprehensively appraised, newfound mechanism explained. Specifically, defect model rabbit condyles, Sim-3DTi exhibited enhanced osteogenesis, in-growth, osseointegration compared alone, greater morphogenetic protein 2 expression. In our nude mice model, loading reduced volume by 59%-77 % without organic damage, implying good activity biosafety. Furthermore, induced ferroptosis upregulating transferrin nicotinamide adenine dinucleotide phosphate oxidase levels both

Язык: Английский

A Review on the Design of Hydrogels With Different Stiffness and Their Effects on Tissue Repair DOI Creative Commons
T. David Luo, Bowen Tan,

Lengjing Zhu

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2022, Номер 10

Опубликована: Янв. 25, 2022

Tissue repair after trauma and infection has always been a difficult problem in regenerative medicine. Hydrogels have become one of the most important scaffolds for tissue engineering due to their biocompatibility, biodegradability water solubility. Especially, stiffness hydrogels is key factor, which influence morphology mesenchymal stem cells (MSCs) differentiation. The researches on this point are meaningful field engineering. Herein, review focus design with different effects behavior MSCs. In addition, effect hydrogel phenotype macrophages introduced, then relationship between changes inflammatory response discussed. Finally, future application certain medicine prospected.

Язык: Английский

Процитировано

112
Harnessing Natural Polymers for Nano-Scaffolds in Bone Tissue Engineering: A Comprehensive Overview of Bone Disease Treatment DOI Creative Commons

Sushmita Saurav,

Prashish Sharma,

Anil Kumar

и другие.

Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(1), С. 585 - 611

Опубликована: Янв. 5, 2024

Numerous surgeries are carried out to replace tissues that have been harmed by an illness or accident. Due various surgical interventions and the requirement of bone substitutes, emerging field tissue engineering attempts repair damaged with help scaffolds. These scaffolds act as template for regeneration controlling development new cells. For creation functional organs, there three elements play very crucial role: cells, signals achievement these aims, types natural polymers, like chitosan, chitin, cellulose, albumin silk fibroin, used preparation Scaffolds produced from polymers many advantages: they less immunogenic well being biodegradable, biocompatible, non-toxic cost effective. The hierarchal structure bone, microscale nanoscale, is mostly made up organic inorganic components nanohydroxyapatite collagen components. This review paper summarizes knowledge updates information about use scaffolds, their application in recent research trends area (BTE). article extensively explores related analyze advancement nanotechnology treatment bone-related diseases repair.

Язык: Английский

Процитировано

27
Curcumin-Microsphere/IR820 Hybrid Bifunctional Hydrogels for In Situ Osteosarcoma Chemo-co-Thermal Therapy and Bone Reconstruction DOI
Bowen Tan, Yanting Wu, Yongzhi Wu

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2021, Номер 13(27), С. 31542 - 31553

Опубликована: Июнь 30, 2021

Conventional biomaterial-mediated osteosarcoma therapy mainly focuses on its antitumor effect yet often fails to overcome the problem of post-treatment bone tissue defect repair. Simultaneously, minimally invasive drug delivery methods are becoming spotlights for normal preservation. Herein, an injectable curcumin-microsphere/IR820 coloaded hybrid methylcellulose hydrogel (Cur-MP/IR820 gel) platform was designed and regeneration. In vitro, K7M2wt cells were eradicated by hyperthermia curcumin. Later, sustained release curcumin promoted alkaline phosphatase expression calcium deposition mesenchymal stem cells. vivo, this could reach tumor site via injection turned into due heat sensitivity. Under irradiation 808 nm laser, localized (∼51 °C) generated in 5 min ablate tumor. Meanwhile, thermal-accelerated thermal-increased cell membrane permeability led apoptosis. Tumors photothermal-co-chemotherapy group successfully restrained from day 2 after treatment. After that, reconstruction because released The chemo-co-thermal efficacy osteogenic capacity Cur-MP/IR820 suggest a promising approach treatment provide provoking inspiration treating tumors repairing tissue.

Язык: Английский

Процитировано

76
Strontium-incorporated bioceramic scaffolds for enhanced osteoporosis bone regeneration DOI Creative Commons
Qianju Wu, Longwei Hu, Ran Yan

и другие.

Bone Research, Год журнала: 2022, Номер 10(1)

Опубликована: Авг. 23, 2022

The restoration of bone defects caused by osteoporosis remains a challenge for surgeons. Strontium ranelate has been applied in preventative treatment approaches due to the biological functions trace element strontium (Sr). In this study, we aimed fabricate bioactive scaffolds through Sr incorporation based on our previously developed modified amino-functional mesoporous glass (MBG) and systematically investigate bioactivity resulting scaffold vitro vivo an osteoporotic rat model. results suggested that Sr-incorporated MBG possessed favorable biocompatibility. Moreover, with Sr, osteogenic angiogenic capacities were upregulated vitro. showed achieved better regeneration vessel formation. Furthermore, bioinformatics analysis indicated could reduce reactive oxygen species levels marrow mesenchymal stem cells model activating cAMP/PKA signaling pathway, thus playing anti-osteoporosis role while promoting osteogenesis. This study demonstrated feasibility incorporating elements into provided new insights biomaterial design facilitating osteoporosis.

Язык: Английский

Процитировано

65
Near-infrared light control of GelMA/PMMA/PDA hydrogel with mild photothermal therapy for skull regeneration DOI Open Access
Yanting Wu, Xu Zhang, Bowen Tan

и другие.

Biomaterials Advances, Год журнала: 2022, Номер 133, С. 112641 - 112641

Опубликована: Янв. 7, 2022

Язык: Английский

Процитировано

59
Craniofacial Bone Tissue Engineering: Current Approaches and Potential Therapy DOI Creative Commons

Arbi Aghali

Cells, Год журнала: 2021, Номер 10(11), С. 2993 - 2993

Опубликована: Ноя. 3, 2021

Craniofacial bone defects can result from various disorders, including congenital malformations, tumor resection, infection, severe trauma, and accidents. Successfully regenerating cranial is an integral step to restore craniofacial function. However, challenges managing controlling new tissue formation remain. Current advances in engineering regenerative medicine use innovative techniques address these challenges. The of biomaterials, stromal cells, growth factors have demonstrated promising outcomes vitro vivo. Natural synthetic grafts combined with Mesenchymal Stromal Cells (MSCs) shown encouraging results critical-size defects. One prevalent Bone Morphogenetic Protein-2 (BMP-2). BMP-2 defined as a gold standard factor that enhances Recently, emerging evidence suggested Megakaryocytes (MKs), induced by Thrombopoietin (TPO), show increase osteoblast proliferation mass Furthermore, co-culture study shows mature MKs enhance MSC survival rate while maintaining their phenotype. Therefore, provide insight potential therapy offering safe effective approach

Язык: Английский

Процитировано

57
Carbon-Based Nanomaterials for Bone and Cartilage Regeneration: A Review DOI
Haoyang Liu, Jialin Chen, Sen Qiao

и другие.

ACS Biomaterials Science & Engineering, Год журнала: 2021, Номер 7(10), С. 4718 - 4735

Опубликована: Сен. 29, 2021

As the main load-bearing structure in human body, bone and cartilage are susceptible to damage sports other activities. The repair regeneration of articular have been extensively studied past decades. Traditional approaches widely applied clinical practice, but effect varies from person may cause side effects. With rapid development tissue engineering regenerative medicine, various biomaterials show great potential cartilage. Carbon-based nanomaterials solid materials with different structures properties composed allotropes carbon, which classified into zero-, one-, two-dimensional ones. This Review systemically summarizes types carbon-based nanomaterials, including zero-dimensional (fullerene, carbon dots, nanodiamonds), one-dimensional (carbon nanotubes), (graphenic materials) as well their applications bone, cartilage, osteochondral regeneration. Current limitations future perspectives also discussed.

Язык: Английский

Процитировано

56
Chitosan-based therapeutic systems and their potentials in treatment of oral diseases DOI
Hongyu Gao, Nan Wu,

Nini Wang

и другие.

International Journal of Biological Macromolecules, Год журнала: 2022, Номер 222, С. 3178 - 3194

Опубликована: Окт. 14, 2022

Язык: Английский

Процитировано

56
nHA-loaded gelatin/alginate hydrogel with combined physical and bioactive features for maxillofacial bone repair DOI
Xiaohu Zhou, Jiwei Sun,

Keqi Wo

и другие.

Carbohydrate Polymers, Год журнала: 2022, Номер 298, С. 120127 - 120127

Опубликована: Сен. 17, 2022

Язык: Английский

Процитировано

44
Carboxymethyl chitosan-alginate enhances bone repair effects of magnesium phosphate bone cement by activating the FAK-Wnt pathway DOI Creative Commons
Ling Yu, Tian Gao, Wei Li

и другие.

Bioactive Materials, Год журнала: 2022, Номер 20, С. 598 - 609

Опубликована: Июль 1, 2022

There is a continuing need for artificial bone substitutes repair and reconstruction, Magnesium phosphate cement (MPC) has exceptional degradable properties exhibits promising biocompatibility. However, its mechanical strength needs improved low osteo-inductive potential limits therapeutic application in regeneration. We functionally modified MPC by using polymeric carboxymethyl chitosan-sodium alginate (CMCS/SA) gel network. This had the advantages of: compressive strength, ease of handling, an optimized interface bioactive in-growth. The new composites with 2% CMCS/SA showed most favorable physicochemical properties, including wash-out resistance, setting time, injectable time heat release. Biologically, composite promoted attachment proliferation osteoblast cells. It was also found to induce osteogenic differentiation vitro, as verified expression markers. In terms molecular mechanisms, data that activated Wnt pathway through inhibition phosphorylation β-catenin, which dependent on focal adhesion kinase. Through micro-computed tomography histological analysis, we MPC-CMCS/SA scaffolds, compared alone, increased regeneration rat calvarial defect model. Overall, our study suggested novel help critical defects clinical practice.

Язык: Английский

Процитировано

42