Antimicrobial Peptide Synergies for Fighting Infectious Diseases

Advanced Science, Год журнала: 2023, Номер 10(26)

Опубликована: Июль 5, 2023

Abstract Antimicrobial peptides (AMPs) are essential elements of thehost defense system. Characterized by heterogenous structures and broad‐spectrumaction, they promising candidates for combating multidrug resistance. Thecombined use AMPs with other antimicrobial agents provides a new arsenal ofdrugs synergistic action, thereby overcoming the drawback monotherapiesduring infections. kill microbes via pore formation, thus inhibitingintracellular functions. This mechanism action is an advantage overantibiotics as it hinders development drug The synergisticeffect will allow repurposing conventional antimicrobials andenhance their clinical outcomes, reduce toxicity, and, most significantly,prevent In this review, various synergies ofAMPs miscellaneous discussed. effect ofstructural diversity chemical modification on AMP properties firstaddressed then different combinations that can lead to action,whether combination between antimicrobials, or andmiscellaneous compounds, attended. review serve guidance whenredesigning in antimicrobialagents enhanced outcomes.

Язык: Английский

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Biofilm Formation, and Related Impacts on Healthcare, Food Processing and Packaging, Industrial Manufacturing, Marine Industries, and Sanitation–A Review

Applied Microbiology, Год журнала: 2023, Номер 3(3), С. 629 - 665

Опубликована: Июнь 26, 2023

Biofilm formation can lead to problems in healthcare, water distribution systems, food processing and packaging, industrial manufacturing, marine industries, sanitation. These microbial communities proliferate on biotic or abiotic surfaces, are responsible for human disease decreasing production efficiency service equipment life many fields. The of biofilm starts with the attachment bacteria surface, followed by bacterial proliferation maturation community. After forming a biofilm, not resistant antimicrobial agents their planktonic forms turn resistant. antibiotic resistance association biofilms generating infectious diseases humans, highlight need designing novel successful antibacterial, anti-biofilm, anti-infection materials. This paper aims review mechanism formation, impact different interaction nanoparticles bacteria, strategies design anti-biofilm Examples bio-implants, coatings, medical devices, wound dressings, sutures reviewed.

Язык: Английский

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Recent applications of vinyl sulfone motif in drug design and discovery

European Journal of Medicinal Chemistry, Год журнала: 2022, Номер 234, С. 114255 - 114255

Опубликована: Март 8, 2022

Язык: Английский

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Artificial Intelligence for Antimicrobial Resistance Prediction: Challenges and Opportunities towards Practical Implementation

Antibiotics, Год журнала: 2023, Номер 12(3), С. 523 - 523

Опубликована: Март 6, 2023

Antimicrobial resistance (AMR) is emerging as a potential threat to many lives worldwide. It very important understand and apply effective strategies counter the impact of AMR its mutation from medical treatment point view. The intersection artificial intelligence (AI), especially deep learning/machine learning, has led new direction in antimicrobial identification. Furthermore, presently, availability huge amounts data multiple sources made it more use these techniques identify interesting insights into genes such genes, mutations, drug identification, conditions favorable spread, so on. Therefore, this paper presents review state-of-the-art challenges opportunities. These include input features posing use, deep-learning/machine-learning models for robustness high accuracy, challenges, prospects practical purposes. concludes with encouragement AI sector intention diagnosis treatment, since presently most studies are at early stages minimal application practice disease.

Язык: Английский

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Cell-free biosynthesis combined with deep learning accelerates de novo-development of antimicrobial peptides

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Ноя. 8, 2023

Bioactive peptides are key molecules in health and medicine. Deep learning holds a big promise for the discovery design of bioactive peptides. Yet, suitable experimental approaches required to validate candidates high throughput at low cost. Here, we established cell-free protein synthesis (CFPS) pipeline rapid inexpensive production antimicrobial (AMPs) directly from DNA templates. To our platform, used deep thousands AMPs de novo. Using computational methods, prioritized 500 that produced screened with CFPS pipeline. We identified 30 functional AMPs, which characterized further through molecular dynamics simulations, activity toxicity. Notably, six novo-AMPs feature broad-spectrum against multidrug-resistant pathogens do not develop bacterial resistance. Our work demonstrates potential low-cost testing within less than 24 h.

Язык: Английский

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CRISPR-Cas-Based Antimicrobials: Design, Challenges, and Bacterial Mechanisms of Resistance

ACS Infectious Diseases, Год журнала: 2023, Номер 9(7), С. 1283 - 1302

Опубликована: Июнь 22, 2023

The emergence of antibiotic-resistant bacterial strains is a source public health concern across the globe. As discovery new conventional antibiotics has stalled significantly over past decade, there an urgency to develop novel approaches address drug resistance in infectious diseases. use CRISPR-Cas-based system for precise elimination targeted populations holds promise as innovative approach antimicrobial agent design. CRISPR-Cas targeting celebrated its high versatility and specificity, offering excellent opportunity fight antibiotic pathogens by selectively inactivating genes involved resistance, biofilm formation, pathogenicity, virulence, or viability. strategy can enact effects two approaches: inactivation chromosomal curing plasmids encoding resistance. In this Review, we provide overview main systems utilized creation these antimicrobials, well highlighting promising studies field. We also offer detailed discussion about most commonly used mechanisms delivery: bacteriophages, nanoparticles, conjugative plasmids. Lastly, possible interference that should be considered during intelligent design approaches.

Язык: Английский

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An antibiotic from an uncultured bacterium binds to an immutable target

Cell, Год журнала: 2023, Номер 186(19), С. 4059 - 4073.e27

Опубликована: Авг. 22, 2023

Antimicrobial resistance is a leading mortality factor worldwide. Here, we report the discovery of clovibactin, an antibiotic isolated from uncultured soil bacteria. Clovibactin efficiently kills drug-resistant Gram-positive bacterial pathogens without detectable resistance. Using biochemical assays, solid-state nuclear magnetic resonance, and atomic force microscopy, dissect its mode action. blocks cell wall synthesis by targeting pyrophosphate multiple essential peptidoglycan precursors (C55PP, lipid II, IIIWTA). uses unusual hydrophobic interface to tightly wrap around but bypasses variable structural elements precursors, accounting for lack Selective efficient target binding achieved sequestration into supramolecular fibrils that only form on membranes contain lipid-anchored groups. This potent holds promise enabling design improved therapeutics kill development.

Язык: Английский

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Glycomimetics for the inhibition and modulation of lectins

Chemical Society Reviews, Год журнала: 2023, Номер 52(11), С. 3663 - 3740

Опубликована: Янв. 1, 2023

Carbohydrates are essential mediators of many processes in health and disease. The development glycomimetics as inhibitors modulators their lectin receptors has been a success story with numerous molecules reaching clinical stages.

Язык: Английский

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Tackling the outer membrane: facilitating compound entry into Gram-negative bacterial pathogens

npj Antimicrobials and Resistance, Год журнала: 2023, Номер 1(1)

Опубликована: Дек. 20, 2023

Emerging resistance to all available antibiotics highlights the need develop new with novel mechanisms of action. Most currently used target Gram-positive bacteria while Gram-negative easily bypass action most drug molecules because their unique outer membrane. This additional layer acts as a potent barrier restricting entry compounds into cell. In this scenario, several approaches have been elucidated increase accumulation bacteria. review includes brief description physicochemical properties that can aid enter and accumulate in covers different strategies or membrane-mediated bacterial pathogens.

Язык: Английский

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Global Strategies to Combat Antimicrobial Resistance: A One Health Perspective

Pharmacology &amp Pharmacy, Год журнала: 2023, Номер 14(08), С. 271 - 328

Опубликована: Янв. 1, 2023

Background: Antimicrobial resistance (AMR) is a global health challenge that has escalated due to the inappropriate use of antimicrobials in humans, animals, and environment. Developing implementing strategies reduce combat AMR critical. Purpose: This study aimed highlight some can be implemented address using One Health approach. Methods: employed narrative review design included studies published from January 2002 July 2023. The searched for literature on antimicrobial stewardship (AMS) PubMed Google Scholar 2020 PRISMA guidelines. Results: reveals remains significant public problem. Its severity been markedly exacerbated by broader ecological Several have developed AMR, including Global Action Plan (GAP), National Plans (NAPs), AMS programs, implementation AWaRe classification antimicrobials. These also involve strengthening surveillance consumption resistance, encouraging development new antimicrobials, enhancing regulations around prescribing, dispensing, usage. Additional measures include promoting partnerships, combating substandard falsified advocating vaccinations, sanitation, hygiene biosecurity, as well exploring alternatives However, these faces various challenges. challenges low awareness knowledge shortage human resources capacity building AMS, adequate funding initiatives, limited laboratory capacities surveillance, behavioural change issues, ineffective leadership multidisciplinary teams. Conclusion: In conclusion, this established prevalent among Successfully addressing calls collaborative, multifaceted Despite this, gaps remain effectively currently recommended AMR. As result, it essential reinforce are deployed counter across human, animal, environmental sectors.

Язык: Английский

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