FEMS Microbiology Letters,
Год журнала:
2020,
Номер
367(17)
Опубликована: Авг. 13, 2020
Methanogenesis
is
the
final
step
in
anaerobic
degradation
of
organic
matter.
The
most
important
substrates
methanogens
are
hydrogen
plus
carbon
dioxide
and
acetate,
but
also
use
methanol,
methylated
amines,
aromatic
methoxy
groups
appears
to
be
more
widespread
than
originally
thought.
Except
for
members
family
Methanosarcinaceae,
all
methylotrophic
require
external
as
reductant
therefore
compete
with
hydrogenotrophic
this
common
substrate.
Since
methanogenesis
from
consumes
four
molecules
per
molecule
methane,
whereas
methanol
requires
only
one,
methyl-reducing
should
have
an
energetic
advantage
over
at
low
partial
pressures.
However,
experimental
data
on
their
threshold
scarce
suffers
relatively
high
detection
limits.
Here,
we
show
that
Methanosphaera
stadtmanae
(Methanobacteriales),
Methanimicrococcus
blatticola
(Methanosarcinales),
Methanomassiliicoccus
luminyensis
(Methanomassiliicoccales)
consume
pressures
<
0.1
Pa,
which
almost
one
order
magnitude
lower
thresholds
M.
reported
previous
study
topic.
We
conclude
outcompete
activity
limited
by
availability
methyl
groups.
The ISME Journal,
Год журнала:
2021,
Номер
15(7), С. 1879 - 1892
Опубликована: Апрель 6, 2021
Abstract
The
classification
of
life
forms
into
a
hierarchical
system
(taxonomy)
and
the
application
names
to
this
hierarchy
(nomenclature)
is
at
turning
point
in
microbiology.
unprecedented
availability
genome
sequences
means
that
taxonomy
can
be
built
upon
comprehensive
evolutionary
framework,
longstanding
goal
taxonomists.
However,
there
resistance
adopting
single
framework
preserve
taxonomic
freedom,
ever
increasing
numbers
genomes
derived
from
uncultured
prokaryotes
threaten
overwhelm
current
nomenclatural
practices,
which
are
based
on
characterised
isolates.
challenge
ahead
then
reach
consensus
adapt
scale
existing
code,
or
create
new
systematically
incorporate
taxa
chosen
framework.
Microorganisms,
Год журнала:
2022,
Номер
10(3), С. 540 - 540
Опубликована: Фев. 28, 2022
Microbial
communities
play
important
roles
in
soil
health,
contributing
to
processes
such
as
the
turnover
of
organic
matter
and
nutrient
cycling.
As
edaphic
properties
chemical
composition
physical
structure
change
from
surface
layers
deeper
ones,
microbiome
similarly
exhibits
substantial
variability
with
depth,
respect
both
community
functional
profiles.
However,
studies
often
neglect
soils,
instead
focusing
on
top
layer
soil.
Here,
we
provide
a
synthesis
how
its
resident
depth.
We
touch
upon
physicochemical
properties,
microbial
diversity,
composition,
profiles,
special
emphasis
carbon
In
doing
so,
seek
highlight
importance
incorporating
analyses
soils
studies.
Abstract
Background
Termites
primarily
feed
on
lignocellulose
or
soil
in
association
with
specific
gut
microbes.
The
functioning
of
the
termite
microbiota
is
partly
understood
a
handful
wood-feeding
pest
species
but
remains
largely
unknown
other
taxa.
We
intend
to
fill
this
gap
and
provide
global
understanding
functional
evolution
microbiota.
Results
sequenced
metagenomes
145
samples
representative
diversity.
show
that
prokaryotic
fraction
all
termites
possesses
similar
genes
for
carbohydrate
nitrogen
metabolisms,
proportions
varying
phylogenetic
position
diet.
presence
conserved
set
implies
essential
nutritional
functions
were
present
ancestor
modern
termites.
Furthermore,
abundance
these
correlated
host
phylogeny.
Finally,
we
found
adaptation
diet
by
some
lineages
was
accompanied
change
stoichiometry
involved
important
rather
than
acquisition
new
pathways.
Conclusions
Our
results
reveal
composition
function
communities
have
been
remarkably
since
first
appeared
~
150
million
years
ago.
Therefore,
“world’s
smallest
bioreactor”
has
operating
as
multipartite
symbiosis
composed
termites,
archaea,
bacteria,
cellulolytic
flagellates
its
inception.
Annual Review of Microbiology,
Год журнала:
2022,
Номер
76(1), С. 727 - 755
Опубликована: Июнь 27, 2022
Methane
is
one
of
the
most
important
greenhouse
gases
on
Earth
and
holds
an
place
in
global
carbon
cycle.
Archaea
are
only
organisms
that
use
methanogenesis
to
produce
energy
rely
methyl-coenzyme
M
reductase
complex
(Mcr).
Over
last
decade,
new
results
have
significantly
reshaped
our
view
diversity
methane-related
pathways
Archaea.
Many
lineages
synthesize
or
methane
been
identified
across
whole
archaeal
tree,
leading
a
greatly
expanded
substrates
mechanisms.
In
this
review,
we
present
state
art
these
advances
how
they
challenge
established
scenarios
origin
evolution
methanogenesis,
discuss
potential
trajectories
may
led
strikingly
wide
range
metabolisms.
Proceedings of the National Academy of Sciences,
Год журнала:
2022,
Номер
119(9)
Опубликована: Фев. 14, 2022
Marine
coastlines
colonized
by
seagrasses
are
a
net
source
of
methane
to
the
atmosphere.
However,
emissions
from
these
environments
still
poorly
constrained,
and
underlying
processes
responsible
microorganisms
remain
largely
unknown.
Here,
we
investigated
turnover
in
seagrass
meadows
Cell Reports Methods,
Год журнала:
2023,
Номер
3(1), С. 100383 - 100383
Опубликована: Янв. 1, 2023
Multi-omics
data
integration
via
mechanistic
models
of
metabolism
is
a
scalable
and
flexible
framework
for
exploring
biological
hypotheses
in
microbial
systems.
However,
although
most
microorganisms
are
unculturable,
such
multi-omics
modeling
limited
to
isolate
microbes
or
simple
synthetic
communities.
Here,
we
developed
an
approach
activity
interactions
that
leverages
the
reconstruction
metagenome-assembled
genomes
associated
genome-centric
metatranscriptomes.
At
its
core,
designed
method
condition-specific
metabolic
communities
through
metatranscriptomic
data.
Using
this
approach,
explored
behavior
anaerobic
digestion
consortia
driven
by
hydrogen
availability
human
gut
microbiota
dysbiosis
with
Crohn's
disease,
identifying
condition-dependent
amino
acid
requirements
archaeal
species
reduced
short-chain
fatty
exchange
network
respectively.
Our
can
be
applied
complex
communities,
allowing
contextualization
on
metagenome
scale.
