Engineering organoids

Nature Reviews Materials, Год журнала: 2021, Номер 6(5), С. 402 - 420

Опубликована: Фев. 19, 2021

Язык: Английский

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Organoids

Nature Reviews Methods Primers, Год журнала: 2022, Номер 2(1)

Опубликована: Дек. 1, 2022

Organoids have attracted increasing attention because they are simple tissue-engineered cell-based in vitro models that recapitulate many aspects of the complex structure and function corresponding vivo tissue. They can be dissected interrogated for fundamental mechanistic studies on development, regeneration, repair human tissues. also used diagnostics, disease modeling, drug discovery, personalized medicine. derived from either pluripotent or tissue-resident stem (embryonic adult) progenitor differentiated cells healthy diseased tissues, such as tumors. To date, numerous organoid engineering strategies support culture growth, proliferation, differentiation maturation been reported. This Primer serves to highlight rationale underlying selection development these materials methods control cellular/tissue niche; therefore, engineered organoid. We discuss key considerations generating robust organoids, those related cell isolation seeding, matrix soluble factor selection, physical cues integration. The general standards data quality, reproducibility deposition within community is outlined. Lastly, we conclude by elaborating limitations organoids different applications, priorities coming years.

Язык: Английский

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Nanodelivery of nucleic acids

Nature Reviews Methods Primers, Год журнала: 2022, Номер 2(1)

Опубликована: Апрель 14, 2022

Язык: Английский

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Detection of Viral Infections by Innate Immunity

Biochemical Pharmacology, Год журнала: 2020, Номер 183, С. 114316 - 114316

Опубликована: Ноя. 2, 2020

Pattern recognition receptors (PRRs) and inflammasomes are a key part of the anti-viral innate immune system as they detect conserved viral pathogen-associated molecular patterns (PAMPs). A successful host response to infections critically depend on initial activation PRRs by viruses, mainly DNA RNA. The signalling pathways activated leads expression pro-inflammatory cytokines, recruit cells, type I III interferons which induction interferon stimulated genes (ISG), powerful virus restriction factors that establish "antiviral state". Inflammasomes contribute responses through maturation interleukin (IL)-1 IL-18 triggering pyroptotic cell death. activity along with adaptive normally elimination, although disproportionate pathology. In this review we will discuss recent insights into influence PRR what means for mammalian host. We also comment how specific may be relevant SARS-CoV-2, responsible current COVID-19 pandemic, interacts immunity.

Язык: Английский

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Towards organoid culture without Matrigel

Communications Biology, Год журнала: 2021, Номер 4(1)

Опубликована: Дек. 10, 2021

Organoids-cellular aggregates derived from stem or progenitor cells that recapitulate organ function in miniature-are of growing interest developmental biology and medicine. Organoids have been developed for organs tissues such as the liver, gut, brain, pancreas; they are used surrogates to study a wide range questions basic biology, genetic disorders, therapies. However, many organoids reported date cultured Matrigel, which is prepared secretion Engelbreth-Holm-Swarm mouse sarcoma cells; Matrigel complex poorly defined. This complexity makes it difficult elucidate Matrigel-specific factors governing organoid development. In this review, we discuss promising Matrigel-free methods generation maintenance use decellularized extracellular matrix (ECM), synthetic hydrogels, gel-forming recombinant proteins.

Язык: Английский

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Building consensus on definition and nomenclature of hepatic, pancreatic, and biliary organoids

Cell stem cell, Год журнала: 2021, Номер 28(5), С. 816 - 832

Опубликована: Май 1, 2021

Язык: Английский

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Vascularized organoids on a chip: strategies for engineering organoids with functional vasculature

Lab on a Chip, Год журнала: 2021, Номер 21(3), С. 473 - 488

Опубликована: Янв. 1, 2021

Possible strategy to integrate pre-vascularized organoid and in vitro capillary bed on a microfluidic based platform, aiming for establishing perfused vasculature throughout organoids vitro.

Язык: Английский

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Volumetric Bioprinting of Organoids and Optically Tuned Hydrogels to Build Liver‐Like Metabolic Biofactories

Advanced Materials, Год журнала: 2022, Номер 34(15)

Опубликована: Фев. 15, 2022

Organ- and tissue-level biological functions are intimately linked to microscale cell-cell interactions the overarching tissue architecture. Together, biofabrication organoid technologies offer unique potential engineer multi-scale living constructs, with cellular microenvironments formed by stem cell self-assembled structures embedded in customizable bioprinted geometries. This study introduces volumetric bioprinting of complex organoid-laden which capture key human liver. Volumetric via optical tomography shapes gelatin hydrogels into centimeter-scale 3D under 20 s. Optically tuned bioresins enable refractive index matching specific intracellular structures, countering disruptive impact cell-mediated light scattering on printing resolution. layerless, nozzle-free technique poses no harmful mechanical stresses organoids, resulting superior viability morphology preservation post-printing. Bioprinted organoids undergo hepatocytic differentiation showing albumin synthesis, liver-specific enzyme activity, remarkably acquired native-like polarization. Organoids within low stiffness gelatins (<2 kPa) mathematically defined lattices varying degrees pore network tortuosity, cultured perfusion. These act as metabolic biofactories ammonia detoxification can be enhanced architectural profile constructs. technology opens up new possibilities for regenerative medicine personalized drug testing.

Язык: Английский

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Human cerebral organoids — a new tool for clinical neurology research

Nature Reviews Neurology, Год журнала: 2022, Номер 18(11), С. 661 - 680

Опубликована: Окт. 17, 2022

Язык: Английский

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Human neural tube morphogenesis in vitro by geometric constraints

Nature, Год журнала: 2021, Номер 599(7884), С. 268 - 272

Опубликована: Окт. 27, 2021

Язык: Английский

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