Organoids: The current status and biomedical applications DOI Creative Commons
Siqi Yang,

Hai‐Jie Hu,

Heng‐Chung Kung

et al.

MedComm, Journal Year: 2023, Volume and Issue: 4(3)

Published: May 17, 2023

Organoids are three-dimensional (3D) miniaturized versions of organs or tissues that derived from cells with stem potential and can self-organize differentiate into 3D cell masses, recapitulating the morphology functions their in vivo counterparts. Organoid culture is an emerging technology, organoids various tissues, such as brain, lung, heart, liver, kidney, have been generated. Compared traditional bidimensional culture, organoid systems unique advantage conserving parental gene expression mutation characteristics, well long-term maintenance function biological characteristics vitro. All these features open up new opportunities for drug discovery, large-scale screening, precision medicine. Another major application disease modeling, especially hereditary diseases difficult to model vitro modeled by combining genome editing technologies. Herein, we introduce development current advances technology field. We focus on applications basic biology clinical research, also highlight limitations future perspectives. hope this review provide a valuable reference developments organoids.

Language: Английский

Engineering organoids DOI Open Access
Moritz Hofer, Matthias P. Lütolf

Nature Reviews Materials, Journal Year: 2021, Volume and Issue: 6(5), P. 402 - 420

Published: Feb. 19, 2021

Language: Английский

Citations

803
Organoids DOI Creative Commons
Zixuan Zhao, Xinyi Chen, Anna M. Dowbaj

et al.

Nature Reviews Methods Primers, Journal Year: 2022, Volume and Issue: 2(1)

Published: Dec. 1, 2022

Organoids have attracted increasing attention because they are simple tissue-engineered cell-based in vitro models that recapitulate many aspects of the complex structure and function corresponding vivo tissue. They can be dissected interrogated for fundamental mechanistic studies on development, regeneration, repair human tissues. also used diagnostics, disease modeling, drug discovery, personalized medicine. derived from either pluripotent or tissue-resident stem (embryonic adult) progenitor differentiated cells healthy diseased tissues, such as tumors. To date, numerous organoid engineering strategies support culture growth, proliferation, differentiation maturation been reported. This Primer serves to highlight rationale underlying selection development these materials methods control cellular/tissue niche; therefore, engineered organoid. We discuss key considerations generating robust organoids, those related cell isolation seeding, matrix soluble factor selection, physical cues integration. The general standards data quality, reproducibility deposition within community is outlined. Lastly, we conclude by elaborating limitations organoids different applications, priorities coming years.

Language: Английский

Citations

454
Nanodelivery of nucleic acids DOI Open Access
Bárbara B. Mendes, João Conniot, Aviram Avital

et al.

Nature Reviews Methods Primers, Journal Year: 2022, Volume and Issue: 2(1)

Published: April 14, 2022

Language: Английский

Citations

382
Detection of Viral Infections by Innate Immunity DOI Creative Commons
Michael Carty,

Coralie Guy,

Andrew Bowie

et al.

Biochemical Pharmacology, Journal Year: 2020, Volume and Issue: 183, P. 114316 - 114316

Published: Nov. 2, 2020

Pattern recognition receptors (PRRs) and inflammasomes are a key part of the anti-viral innate immune system as they detect conserved viral pathogen-associated molecular patterns (PAMPs). A successful host response to infections critically depend on initial activation PRRs by viruses, mainly DNA RNA. The signalling pathways activated leads expression pro-inflammatory cytokines, recruit cells, type I III interferons which induction interferon stimulated genes (ISG), powerful virus restriction factors that establish "antiviral state". Inflammasomes contribute responses through maturation interleukin (IL)-1 IL-18 triggering pyroptotic cell death. activity along with adaptive normally elimination, although disproportionate pathology. In this review we will discuss recent insights into influence PRR what means for mammalian host. We also comment how specific may be relevant SARS-CoV-2, responsible current COVID-19 pandemic, interacts immunity.

Language: Английский

Citations

300
Towards organoid culture without Matrigel DOI Creative Commons
Mark T. Kozlowski, Christiana Crook, Hsun Teresa Ku

et al.

Communications Biology, Journal Year: 2021, Volume and Issue: 4(1)

Published: Dec. 10, 2021

Organoids-cellular aggregates derived from stem or progenitor cells that recapitulate organ function in miniature-are of growing interest developmental biology and medicine. Organoids have been developed for organs tissues such as the liver, gut, brain, pancreas; they are used surrogates to study a wide range questions basic biology, genetic disorders, therapies. However, many organoids reported date cultured Matrigel, which is prepared secretion Engelbreth-Holm-Swarm mouse sarcoma cells; Matrigel complex poorly defined. This complexity makes it difficult elucidate Matrigel-specific factors governing organoid development. In this review, we discuss promising Matrigel-free methods generation maintenance use decellularized extracellular matrix (ECM), synthetic hydrogels, gel-forming recombinant proteins.

Language: Английский

Citations

249
Building consensus on definition and nomenclature of hepatic, pancreatic, and biliary organoids DOI Creative Commons
Ary Marsee, Floris J.M. Roos, Monique M.A. Verstegen

et al.

Cell stem cell, Journal Year: 2021, Volume and Issue: 28(5), P. 816 - 832

Published: May 1, 2021

Language: Английский

Citations

219
Vascularized organoids on a chip: strategies for engineering organoids with functional vasculature DOI Creative Commons
Shun Zhang, Zhengpeng Wan,

Roger D. Kamm

et al.

Lab on a Chip, Journal Year: 2021, Volume and Issue: 21(3), P. 473 - 488

Published: Jan. 1, 2021

Possible strategy to integrate pre-vascularized organoid and in vitro capillary bed on a microfluidic based platform, aiming for establishing perfused vasculature throughout organoids vitro.

Language: Английский

Citations

207
Volumetric Bioprinting of Organoids and Optically Tuned Hydrogels to Build Liver‐Like Metabolic Biofactories DOI Creative Commons
Paulina Núñez Bernal, Manon Bouwmeester, Jorge Madrid‐Wolff

et al.

Advanced Materials, Journal Year: 2022, Volume and Issue: 34(15)

Published: Feb. 15, 2022

Organ- and tissue-level biological functions are intimately linked to microscale cell-cell interactions the overarching tissue architecture. Together, biofabrication organoid technologies offer unique potential engineer multi-scale living constructs, with cellular microenvironments formed by stem cell self-assembled structures embedded in customizable bioprinted geometries. This study introduces volumetric bioprinting of complex organoid-laden which capture key human liver. Volumetric via optical tomography shapes gelatin hydrogels into centimeter-scale 3D under 20 s. Optically tuned bioresins enable refractive index matching specific intracellular structures, countering disruptive impact cell-mediated light scattering on printing resolution. layerless, nozzle-free technique poses no harmful mechanical stresses organoids, resulting superior viability morphology preservation post-printing. Bioprinted organoids undergo hepatocytic differentiation showing albumin synthesis, liver-specific enzyme activity, remarkably acquired native-like polarization. Organoids within low stiffness gelatins (<2 kPa) mathematically defined lattices varying degrees pore network tortuosity, cultured perfusion. These act as metabolic biofactories ammonia detoxification can be enhanced architectural profile constructs. technology opens up new possibilities for regenerative medicine personalized drug testing.

Language: Английский

Citations

172
Human cerebral organoids — a new tool for clinical neurology research DOI Open Access
Oliver L. Eichmüller, Juergen A. Knoblich

Nature Reviews Neurology, Journal Year: 2022, Volume and Issue: 18(11), P. 661 - 680

Published: Oct. 17, 2022

Language: Английский

Citations

168
Cell Therapy: Types, Regulation, and Clinical Benefits DOI Creative Commons
Abed El-Hakim El-Kadiry, Moutih Rafei,

Riam Shammaa

et al.

Frontiers in Medicine, Journal Year: 2021, Volume and Issue: 8

Published: Nov. 22, 2021

Cell therapy practices date back to the 19 th century and continue expand on investigational investment grounds. includes stem cell- non–stem cell-based, unicellular multicellular therapies, with different immunophenotypic profiles, isolation techniques, mechanisms of action, regulatory levels. Following steps their predecessor cell therapies that have become established or commercialized, premarket approval-exempt provide patients promising therapeutic benefits in disease areas. In this review article, we delineate vast types therapy, including cell-based create first-in-literature compilation “multicellular” used clinical settings. Besides providing nuts bolts FDA policies regulating use, discuss reported 3 areas—regenerative medicine, immune diseases, cancer. Finally, contemplate recent attention shift toward combined approaches, highlighting factors render a more attractive option than counterparts.

Language: Английский

Citations

160