Environmental Sustainability, Год журнала: 2020, Номер 3(4), С. 453 - 469
Опубликована: Окт. 23, 2020
Язык: Английский
Trends in Ecology & Evolution, Год журнала: 2022, Номер 37(6), С. 517 - 528
Опубликована: Март 1, 2022
Язык: Английский
Процитировано
116
Nature Astronomy, Год журнала: 2021, Номер 5(7), С. 665 - 675
Опубликована: Июнь 28, 2021
Язык: Английский
Процитировано
66
Frontiers in Genetics, Год журнала: 2020, Номер 11
Опубликована: Ноя. 5, 2020
Several cyanobacterial species are dominant primary producers in hot spring microbial mats. To date, taxonomy, as well the evolution of their genomic adaptations to high temperatures, poorly understood, with information currently available for only a few genera, including Fischerella and Synechococcus. address this knowledge gap, present study expands landscape cyanobacteria traces phylum-wide consequences temperature environments. From 21 globally distributed metagenomes, temperatures between 32 75°C, 57 medium- high-quality metagenome-assembled genomes were recovered, representing taxonomic novelty 1 order, 3 families, 15 genera 36 species. Comparative genomics 93 (including genomes) 66 non-thermal genomes, showed that former have smaller higher GC content, shorter proteins more hydrophilic basic, when compared genomes. Additionally, core accessory orthogroups from some had greater abundance functional categories, such inorganic ion metabolism, translation post-translational modifications. Moreover, increased abundances transport amino acid less replication transcription functions protein coding sequences. Furthermore, they dependence on CRISPR-Cas defense system against exogenous nucleic acids, reduction secondary metabolism biosynthetic gene clusters. This suggests differences response environment-specific communities. provides new insights into specific niche where dominant, which could be essential trace bacterial pathways warmer world, current global warming scenario.
Язык: Английский
Процитировано
49
Scientific Reports, Год журнала: 2025, Номер 15(1)
Опубликована: Фев. 14, 2025
Abstract Stromatolites can be traced back to ∼3.5 billion years. They were widespread in the shorelines of ancient oceans and seas. However, they are uncommon nowadays, basic information is lacking about how these unique carbonate structures developed. Here we study unusually thick (3–5 cm) biofilms 79.2 °C outflow from Köröm thermal well (Hungary) demonstrate that its microbial mat – architecture similar fossilized microdigitate stromatolites. Our observations reveal vertically oriented fibrous mineral fabrics, typical stromatolites, red biofilm clotted mesostructures, thrombolites, green biofilm. These layers contain peloids show network structures, formed by filamentous microbes. The 16S rRNA gene-based amplicon sequencing implies numerous undescribed taxa may contribute mineralisation. abundantly phyla Bacteroidota, Pseudomonadota Cyanobacteria. Geitlerinema PCC-8501 Raineya characteristic for biofilm, whereas uncultured Oxyphotobacteria, unc. Saprospiraceae Cytophagales abundant A hydrogen-oxidizing Hydrogenobacter within phylum Aquificota unclassified Bacteria together with Deinococcota dominate water samples. morphological structure taxonomic composition a representation development processes microbialite formations.
Язык: Английский
Процитировано
0
Frontiers in Genetics, Год журнала: 2021, Номер 12
Опубликована: Март 8, 2021
Hot springs are considered to be a unique environment with extremophiles, that sources of industrially important enzymes, and other biotechnological products. The objective this study was undertake, analyze, characterize the microbiome two major hot located in state Madhya Pradesh explicitly, Chhoti Anhoni (Hotspring 1), Badi 2) find out inhabitant microbial population, their functional characteristics. taxonomic analysis revealed phylum Proteobacteria most abundant taxa both hot-springs, however, its abundance hot-spring 1 (~88%) more than 2 (~52%). Bacteroides (~10–22%) found second group hot-springs followed by Spirocheates (~2–11%), Firmicutes (~6–8%), Chloroflexi (1–5%), etc. different features related several functions including metabolism organics degradation xenobiotic compounds. showed attributes metabolism, cellular processes environmental information processing functions. annotation microbiomes at KEGG level 3 annotated sequences into 279 active variation between spring samples, where functionally diverse. Interestingly, genes from methanogenic bacteria, higher 2, which may relatively pH temperature Hotspring 1. presence unassigned bacterial high indicates potential novel genera or phylotypes. Culturable isolates (28) were bio-prospected for enzymes amylase, protease, lipase, gelatinase, pectinase, cellulase, lecithinase, xylanase. Seven (25%) had shown positive results all enzyme activities whereas 23 (82%) produced Protease, 27 (96%) 26 (92%) 19 (67%) could produce 13 (46%) gelatinase. seven isolates, analyzed further quantitative identified through molecular characterization.
Язык: Английский
Процитировано
28
Environmental Advances, Год журнала: 2022, Номер 7, С. 100166 - 100166
Опубликована: Янв. 7, 2022
The current study evaluated the bacterial diversity of six hot water spring clusters in Sri Lanka by Illumina MiSeq sequencing V3–V4 region 16S rRNA gene. Bacterial abundance measures and statistics were assessed using QIIME2 metagenomics workflow, results compared according to region, temperature at surface (36–59 °C), pH (6.25–8.35). predominant phyla observed Proteobacteria, Actinobacteria, Firmicutes, [Thermi], Cyanobacteria. A low Bacteroidetes, Chloroflexi, Acidobacteria, TM7, Spirochaetes was detected most springs. Several important species such as Deinococcus geothermalis that can tolerate Martian-like conditions, genera Legionella Campylobacter contain pathogenic species, sulfur metabolizing Desulfovibrio, Desulfatirhabdium, Desulforhabdus, Desulfacinum, Thermodesulfovibrio, Desulfovirga, Thiobacter several other with potential practical industrial application detected. opportunistic human pathogens samples raised a public health concern about management post-bathing water. Based on Bray Curtis beta metric, microbial distribution correlated rather than geographic distance. This provides valuable new insights into springs Lanka. Future research needs be conducted industrially thermophiles identified this study.
Язык: Английский
Процитировано
17
ISME Communications, Год журнала: 2023, Номер 3(1)
Опубликована: Авг. 18, 2023
Terrestrial hot springs harbor diverse microbial communities whose compositions are shaped by the wide-ranging physico-chemistries of individual springs. The effect enormous physico-chemical differences on bacterial and archaeal distributions population structures is little understood. We therefore analysed prevalence relative abundance bacteria archaea in sediments (n = 76) spring features, Taupō Volcanic Zone (New Zealand), spanning large major anion water chemistry, pH (2.0-7.5), temperature (17.5-92.9 °C). Community composition, based 16S rRNA amplicon sequence variants (ASVs) was strongly influenced both pH. However, certain lineages characterized At domain level, shared broadly equivalent community abundances across physico-chemically springs, despite slightly lower bacteria-to-archaea ratios gene concentrations at higher temperatures. Communities were almost exclusively dominated Proteobacteria, Euryarchaeota or Crenarchaeota. Eight ASVs from Thermoplasmatales, Desulfurellaceae, Mesoaciditogaceae Acidithiobacillaceae unusually prevalent (present 57.9-84.2% samples) abundant (1.7-12.0% sample abundance), together comprised 44% overall abundance. Metagenomic analyses generated multiple populations associated with dominant ASVs, showed characteristic traits each lineage for sulfur, nitrogen hydrogen metabolism. Differences metabolic composition genome-specific metabolism delineated relatives. Genome coverage calculations that distributed a physicochemically broad range Results imply different potentials colonizing environments.
Язык: Английский
Процитировано
9
Environmental Microbiology, Год журнала: 2021, Номер 23(7), С. 3335 - 3344
Опубликована: Апрель 5, 2021
Astrobiology is mistakenly regarded by some as a field confined to studies of life beyond Earth. Here, we consider on Earth through an astrobiological lens. Whereas classical microbiology historically focused various anthropocentric sub-fields (such fermented foods or commensals and pathogens crop plants, livestock humans), addressing key biological questions via approaches can further our understanding all We highlight potential implications this approach the articles in Environmental Microbiology special issue 'Ecophysiology Extremophiles'. They report places/processes including low-temperature environments chemically diverse saline- hypersaline habitats; aspects sulphur metabolism lakes, dysoxic marine waters, thermal acidic springs; biology extremophile viruses; survival terrestrial extremophiles surface Mars; soils crusts rock-associated microbes deserts; subsurface deep biosphere, salticle formed within Triassic halite; interactions with igneous sedimentary rocks. These studies, which here, contribute spatiotemporal reach Earth'sfunctional tenacity life. Their findings will help set stage for future work constraints life, how organisms adapt evolve circumvent these constraints.
Язык: Английский
Процитировано
21
Ecologies, Год журнала: 2023, Номер 4(1), С. 106 - 123
Опубликована: Фев. 10, 2023
Hot springs are known as highly adverse extreme environments where thermophilic and hyperthermophilic microorganisms can survive. We describe taxonomic diversity of several microbial biofilms collected along water temperature gradient in hot streams the aquatic system Stolbovskie on Kunashir Island, Kurils, Russia. The composition studied communities was assessed by 16S rRNA gene metabarcoding for bacteria archaea, 18S protists. Richness geothermal decreased with increase temperature, while tendency opposite. Ciliophora most represented taxon various kinds that we found a very local area were different from each other composition, level their significantly influenced temperature.
Язык: Английский
Процитировано
8
Biogeosciences, Год журнала: 2020, Номер 17(18), С. 4611 - 4631
Опубликована: Сен. 22, 2020
Abstract. To explore the potential role of tetrathionate in sedimentary sulfur cycle, population ecology microorganisms capable metabolizing this polythionate was revealed at 15–30 cm resolution along two, ∼3 m long, cores collected from 530 and 580 below sea level, off India's west coast, within oxygen minimum zone (OMZ) Arabian Sea. Metagenome analysis widespread occurrence genes involved formation, oxidation, reduction tetrathionate; high diversity relative abundance were also detected for bacteria that are known to render these metabolisms vitro. Results slurry culture sediment samples thiosulfate- or tetrathionate-containing microbial growth media, data obtained via pure-culture isolation, finally metatranscriptome analyses corroborated situ functionality tetrathionate-forming, tetrathionate-oxidizing, tetrathionate-reducing microorganisms. Ion chromatography pore waters presence up 11.1 µM thiosulfate two cores, whereas remained undetected spectroscopic assay based on its reaction with cyanide. While oxidation by chemolithotrophic prevalent is apparent source ecosystem, biochemical geochemical reactivity could be instrumental cryptic status cycle. Potential abiotic origin horizon explored neither ruled out nor confirmed information available. On other hand, potentially present system can either oxidized sulfate reduced back thiosulfate/sulfide respiration native bacterial populations, respectively. Up 2.01 mM sulfide may reduce abiotically elemental sulfur. However, absence measured O2 oxyanions having possibilities serving as electron acceptors, biogeochemical modalities oxidative half cycle unresolved.
Язык: Английский
Процитировано
21