The Analyst, Год журнала: 2022, Номер 147(10), С. 2023 - 2034
Опубликована: Янв. 1, 2022
Microfluidic platforms with 3D tumour spheroid culture provide to study the multi-cellular interactions and external stimuli in complex microenvironment.
Язык: Английский
ACS Nano, Год журнала: 2023, Номер 17(15), С. 14205 - 14228
Опубликована: Июль 27, 2023
The use of nanoparticles (NPs) in nanomedicine holds great promise for the treatment diseases which conventional therapies present serious limitations. Additionally, NPs can drastically improve early diagnosis and follow-up many disorders. However, to harness their full capabilities, they must be precisely designed, produced, tested relevant models. Microfluidic systems simulate dynamic fluid flows, gradients, specific microenvironments, multiorgan complexes, providing an efficient cost-effective approach both synthesis screening. technologies allow under controlled conditions, enhancing batch-to-batch reproducibility. Moreover, due versatility microfluidic devices, it is possible generate customize endless platforms rapid vitro vivo screening NPs' performance. Indeed, devices show potential as advanced small organism manipulation immobilization. In this review, first we summarize major that synthesis. Next, will discuss most innovative enable mimicking environments well give insights into organism-on-a-chip promising application We conclude review with a critical assessment current challenges future directions impact field nanomedicine.
Язык: Английский
Процитировано
125
Biosensors, Год журнала: 2022, Номер 12(11), С. 1023 - 1023
Опубликована: Ноя. 16, 2022
Both passive and active microfluidic chips are used in many biomedical chemical applications to support fluid mixing, particle manipulations, signal detection. Passive devices geometry-dependent, their uses rather limited. Active include sensors or detectors that transduce chemical, biological, physical changes into electrical optical signals. Also, they transduction detect biological applications, highly versatile tools for disease diagnosis organ modeling. This review provides a comprehensive overview of the significant advances have been made development microfluidics devices. We will discuss function as micromixers sorters cells substances (e.g., microfiltration, flow displacement, trapping). Microfluidic fabricated using range techniques, including molding, etching, three-dimensional printing, nanofabrication. Their broad utility lies detection diagnostic biomarkers organ-on-chip approaches permit modeling cancer, well neurological, cardiovascular, hepatic, pulmonary diseases. Biosensor allow point-of-care testing, assays based on enzymes, nanozymes, antibodies, nucleic acids (DNA RNA). An anticipated field includes optimization techniques fabrication biocompatible materials. These developments increase versatility, reduce costs, accelerate time technology.
Язык: Английский
Процитировано
105
TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 173, С. 117640 - 117640
Опубликована: Март 8, 2024
Язык: Английский
Процитировано
71
Experimental & Molecular Medicine, Год журнала: 2024, Номер 56(7), С. 1531 - 1551
Опубликована: Июль 1, 2024
Over the past decade, emergence of patient-derived tumor organoids (PDTOs) has broadened repertoire preclinical models and progressively revolutionized three-dimensional cell culture in oncology. PDTO can be grown from patient samples with high efficiency faithfully recapitulates histological molecular characteristics original tumor. Therefore, PDTOs serve as invaluable tools oncology research, their translation to clinical practice is exciting for future precision medicine In this review, we provide an overview methods establishing various applications cancer starting basic research ending identification new targets validation anticancer compounds medicine. Finally, highlight challenges associated implementation PDTO, such its representativeness, success rate, assay speed, lack a microenvironment. Technological developments autologous cocultures stromal cells are currently ongoing meet these optimally exploit full potential models. The use standard could lead era coming decade.
Язык: Английский
Процитировано
27
TrAC Trends in Analytical Chemistry, Год журнала: 2022, Номер 153, С. 116640 - 116640
Опубликована: Апрель 15, 2022
Язык: Английский
Процитировано
50
Frontiers in Bioengineering and Biotechnology, Год журнала: 2022, Номер 10
Опубликована: Дек. 23, 2022
Microfluidic chip technology is a platform that integrates basic operation units such as processing, separation, reaction and detection into microchannel to realize low consumption, fast efficient analysis of samples. It has the characteristics small volume need samples reagents, analysis, cost, automation, portability, high throughout, good compatibility with other techniques. In this review, concept, preparation materials fabrication microfluidic are described. The applications in immunoassay, including fluorescent, chemiluminescent, surface-enhanced Raman spectroscopy (SERS), electrochemical immunoassay reviewed. Look future, development chips lies point-of-care testing throughput equipment, there still some challenges design integration chips, well actual sample by chips.
Язык: Английский
Процитировано
42
Biosensors and Bioelectronics, Год журнала: 2023, Номер 224, С. 115057 - 115057
Опубликована: Янв. 2, 2023
Язык: Английский
Процитировано
26
Molecular Cancer, Год журнала: 2024, Номер 23(1)
Опубликована: Март 9, 2024
Abstract Despite advancements in treatment protocols, cancer is one of the leading cause deaths worldwide. Therefore, there a need to identify newer and personalized therapeutic targets along with screening technologies combat cancer. With advent pan-omics technologies, such as genomics, transcriptomics, proteomics, metabolomics, lipidomics, scientific community has witnessed an improved molecular metabolomic understanding various diseases, including In addition, three-dimensional (3-D) disease models have been efficiently utilized for pathophysiology tools drug discovery. An integrated approach utilizing 3-D vitro tumor led intricate network encompassing signalling pathways cross-talk solid tumors. present review, we underscore current trends omics highlight their role genotypic-phenotypic co-relation respect models. We further discuss challenges associated provide our outlook on future applications these discovery precision medicine management Graphical
Язык: Английский
Процитировано
16
ACS Sensors, Год журнала: 2024, Номер 9(7), С. 3466 - 3488
Опубликована: Июль 11, 2024
Organ-on-a-Chip (OOC) technology, which emulates the physiological environment and functionality of human organs on a microfluidic chip, is undergoing significant technological advancements. Despite its rapid evolution, this technology also facing notable challenges, such as lack vascularization, development multiorgan-on-a-chip systems, replication body single chip. The progress has played crucial role in steering OOC toward mimicking microenvironment, including microenvironment replication, multiorgan microphysiological systems. Additionally, advancements detection, analysis, organoid imaging technologies have enhanced efficiency Organs-on-Chips (OOCs). In particular, integration artificial intelligence revolutionized imaging, significantly enhancing high-throughput drug screening. Consequently, review covers research Human-on-a-chip, sensors OOCs, latest applications biomedical field.
Язык: Английский
Процитировано
15
Advanced Materials, Год журнала: 2024, Номер unknown
Опубликована: Март 16, 2024
Nanotechnology-based approaches are promising for the treatment of musculoskeletal (MSK) disorders, which present significant clinical burdens and challenges, but their translation requires a deep understanding complex interplay between nanotechnology MSK biology. Organ-on-a-chip (OoC) systems have emerged as an innovative versatile microphysiological platform to replicate dynamics tissue microenvironment studying nanotechnology-biology interactions. This review first covers recent advances applications OoCs ability mimic biophysical biochemical stimuli encountered by tissues. Next, integrating into OoCs, cellular responses behaviors may be investigated precisely controlling manipulating nanoscale environment. Analysis disease mechanisms, particularly bone, joint, muscle degeneration, drug screening development personalized medicine greatly facilitated using OoCs. Finally, future challenges directions outlined field, including advanced sensing technologies, integration immune-active components, enhancement biomimetic functionality. By highlighting emerging this aims advance intricate nanotechnology-MSK biology interface its significance in management, therapeutic interventional strategies.
Язык: Английский
Процитировано
14