Deciphering the catalytic mechanism of superoxide dismutase activity of carbon dot nanozyme

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Янв. 11, 2023

Nanozymes with superoxide dismutase (SOD)-like activity have attracted increasing interest due to their ability scavenge anion, the origin of most reactive oxygen species in vivo. However, SOD nanozymes reported thus far yet approach natural enzymes. Here, we report a carbon dot (C-dot) nanozyme catalytic over 10,000 U/mg, comparable that Through selected chemical modifications and theoretical calculations, show SOD-like C-dots relies on hydroxyl carboxyl groups for binding anions carbonyl conjugated π-system electron transfer. Moreover, C-dot exhibit intrinsic targeting oxidation-damaged cells effectively protect neuron ischemic stroke male mice model. Together, our study sheds light structure-activity relationship nanozymes, demonstrates potential treating oxidation stress related diseases.

Язык: Английский

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Red Emissive Carbon Dot Superoxide Dismutase Nanozyme for Bioimaging and Ameliorating Acute Lung Injury

Advanced Functional Materials, Год журнала: 2023, Номер 33(19)

Опубликована: Янв. 29, 2023

Abstract Harnessing the physiochemical properties and enzymatic activities of nanozymes will provide new insights for disease theranostics. Herein, a novel carbon dot (C‐dot) superoxide dismutase (SOD) nanozyme that exhibits red fluorescence with emission wavelength 683 nm shows high SOD‐like activity >4000 U mg −1 is reported, which presents great potential imaging biodistribution itself in vivo ameliorating acute lung injury. Through surface modifications, mechanism C‐dot SOD revealed to be relied on their functional groups bind radicals, promote electron transfer between C‐dots finally accelerate dismutation radicals. The absolute quantum yield ≈14% endow it bioimaging vitro vivo. Moreover, effectively enters cells, accumulates at mitochondria, protects living cells from oxidative damage by scavenging reactive oxygen species (ROS) reducing levels pro‐inflammatory factors. Importantly, animal experiments demonstrate accumulation injure therapeutic effect toward injury mice. fluorescent management ROS‐related diseases.

Язык: Английский

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Specific Nanodrug for Diabetic Chronic Wounds Based on Antioxidase-Mimicking MOF-818 Nanozymes

Journal of the American Chemical Society, Год журнала: 2022, Номер 144(51), С. 23438 - 23447

Опубликована: Дек. 13, 2022

Chronic wound is a common complication for diabetic patients, which entails substantial inconvenience, persistent pain, and significant economic burden to patients. However, current clinical treatments chronic wounds remain unsatisfactory. A prolonged but ineffective inflammation phase in the primary difference between normal wounds. Herein, we present an effective antioxidative system (MOF/Gel) healing of rats through integrating metal organic framework (MOF) nanozyme with antioxidant enzyme-like activity hydrogel (Gel). MOF/Gel can continuously scavenge reactive oxygen species modulate oxidative stress microenvironment wounds, leads natural transition from proliferation phase. Impressively, efficacy one-time-applied was comparable that human epidermal growth factor Gel, widely used drug various treatments. Such effective, safe, convenient meet complex demands.

Язык: Английский

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Multifunctional mesoporous silica nanoparticles for biomedical applications

Signal Transduction and Targeted Therapy, Год журнала: 2023, Номер 8(1)

Опубликована: Ноя. 24, 2023

Abstract Mesoporous silica nanoparticles (MSNs) are recognized as a prime example of nanotechnology applied in the biomedical field, due to their easily tunable structure and composition, diverse surface functionalization properties, excellent biocompatibility. Over past two decades, researchers have developed wide variety MSNs-based nanoplatforms through careful design controlled preparation techniques, demonstrating adaptability various application scenarios. With continuous breakthroughs MSNs fields biosensing, disease diagnosis treatment, tissue engineering, etc., gradually moving from basic research clinical trials. In this review, we provide detailed summary beginning with comprehensive overview development history. We then discuss types nanostructured architectures, well classification nanocomposites according elements existed inorganic functional components. Subsequently, summarize primary purposes surface-functionalized modifications MSNs. following, applications MSNs, highlight targeted therapeutic modalities currently developed. Given importance translation, also progress Finally, take perspective on future direction remaining challenges field.

Язык: Английский

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The Applications and Mechanisms of Superoxide Dismutase in Medicine, Food, and Cosmetics

Antioxidants, Год журнала: 2023, Номер 12(9), С. 1675 - 1675

Опубликована: Авг. 27, 2023

Superoxide dismutase (SOD) is a class of enzymes that restrict the biological oxidant cluster enzyme system in body, which can effectively respond to cellular oxidative stress, lipid metabolism, inflammation, and oxidation. Published studies have shown SOD (SODs) could maintain dynamic balance between production scavenging oxidants body prevent toxic effects free radicals, been be effective anti-tumor, anti-radiation, anti-aging studies. This research summarizes types, functions, regulatory mechanisms SODs, as well their applications medicine, food production, cosmetic production. SODs proven useful tool fighting disease, mimetics conjugates report developed successively improve effectiveness SODs. There are still obstacles solving membrane permeability persistence action, hot spot difficulty mining effect promoting application future.

Язык: Английский

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Biomedical Applications of Microfluidic Devices: A Review

Biosensors, Год журнала: 2022, Номер 12(11), С. 1023 - 1023

Опубликована: Ноя. 16, 2022

Both passive and active microfluidic chips are used in many biomedical chemical applications to support fluid mixing, particle manipulations, signal detection. Passive devices geometry-dependent, their uses rather limited. Active include sensors or detectors that transduce chemical, biological, physical changes into electrical optical signals. Also, they transduction detect biological applications, highly versatile tools for disease diagnosis organ modeling. This review provides a comprehensive overview of the significant advances have been made development microfluidics devices. We will discuss function as micromixers sorters cells substances (e.g., microfiltration, flow displacement, trapping). Microfluidic fabricated using range techniques, including molding, etching, three-dimensional printing, nanofabrication. Their broad utility lies detection diagnostic biomarkers organ-on-chip approaches permit modeling cancer, well neurological, cardiovascular, hepatic, pulmonary diseases. Biosensor allow point-of-care testing, assays based on enzymes, nanozymes, antibodies, nucleic acids (DNA RNA). An anticipated field includes optimization techniques fabrication biocompatible materials. These developments increase versatility, reduce costs, accelerate time technology.

Язык: Английский

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Reaction Mechanisms and Kinetics of Nanozymes: Insights from Theory and Computation

Advanced Materials, Год журнала: 2023, Номер 36(10)

Опубликована: Янв. 15, 2023

Abstract “Nanozymes” usually refers to inorganic nanomaterials with enzyme‐like catalytic activities. The research into nanozymes is one of the hot topics on horizon interdisciplinary science involving materials, chemistry, and biology. Although great progress has been made in design, synthesis, characterization, application nanozymes, study underlying microscopic mechanisms kinetics still not straightforward. Density functional theory (DFT) calculations compute potential energy surfaces along reaction coordinates for chemical reactions, which can give atomistic‐level insights micro‐mechanisms nanozymes. Therefore, DFT have playing an increasingly important role exploring past years. either predict details processes complement experiments or further develop theoretical models depict physicochemical rules. In this review, corresponding summarized. Particularly, review focuses computational studies that closely interplay experiments. relevant experimental results without will be also briefly discussed offer a historic overview how computations promote understanding

Язык: Английский

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Exploring the Specificity of Nanozymes

ACS Nano, Год журнала: 2024, Номер 18(4), С. 2533 - 2540

Опубликована: Янв. 12, 2024

Nanozymes, nanomaterials exhibiting enzyme-like activities, have emerged as a prominent interdisciplinary field over the past decade. To date, 1200 different been identified nanozymes, covering four catalytic categories: oxidoreductases, hydrolases, isomerases, and lyases. Catalytic activity specificity are two pivotal benchmarks for evaluating enzymatic performance. Despite substantial progress being made in quantifying optimizing of there is still lack in-depth research on preventing formation consensual knowledge impeding more refined systematic classification nanozymes. Recently, debates regarding whether nanozymes could possess similar to that enzymes. This Perspective discusses by referring enzymes, highlights gap between concludes offering our perspective future

Язык: Английский

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Designing nanozymes for in vivo applications

Nature Reviews Bioengineering, Год журнала: 2024, Номер 2(10), С. 849 - 868

Опубликована: Июль 18, 2024

Язык: Английский

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Deep Insight of Design, Mechanism, and Cancer Theranostic Strategy of Nanozymes

Nano-Micro Letters, Год журнала: 2023, Номер 16(1)

Опубликована: Ноя. 21, 2023

Since the discovery of enzyme-like activity Fe

Язык: Английский

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