A Whole‐Course‐Repair System Based on Neurogenesis‐Angiogenesis Crosstalk and Macrophage Reprogramming Promotes Diabetic Wound Healing

Advanced Materials, Год журнала: 2023, Номер 35(19)

Опубликована: Фев. 22, 2023

Diabetic wound (DW) therapy is currently a big challenge in medicine and strategies to enhance neurogenesis angiogenesis have appeared be promising direction. However, the current treatments failed coordinate simultaneously, leading an increased disability rate caused by DWs. Herein, whole-course-repair system introduced hydrogel concurrently achieve mutually supportive cycle of neurogenesis-angiogenesis under favorable immune-microenvironment. This can first one-step packaged syringe for later situ local injections cover wounds long-termly accelerated healing via synergistic effect magnesium ions (Mg2+ ) engineered small extracellular vesicles (sEVs). The self-healing bio-adhesive properties make it ideal physical barrier At inflammation stage, formulation recruit bone marrow-derived mesenchymal stem cells sites stimulate them toward neurogenic differentiation, while providing immune microenvironment macrophage reprogramming. proliferation stage repair, robust occurs newly differentiated neural released Mg2+ , allowing regenerative take place at site. provides novel platform combined DW therapy.

Язык: Английский

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An Immunomodulatory Hydrogel by Hyperthermia‐Assisted Self‐Cascade Glucose Depletion and ROS Scavenging for Diabetic Foot Ulcer Wound Therapeutics

Advanced Materials, Год журнала: 2023, Номер 35(48)

Опубликована: Окт. 7, 2023

Abstract Current therapeutic protocols for diabetic foot ulcers (DFUs), a severe and rapidly growing chronic complication in patients, remain nonspecific. Hyperglycemia‐caused inflammation excessive reactive oxygen species (ROS) are common obstacles encountered DFU wound healing, often leading to impaired recovery. These two effects reinforce each other, forming an endless loop. However, adequate inclusive methods still lacking target these aspects break the vicious cycle. This study proposes novel approach treating wounds, utilizing immunomodulatory hydrogel achieve self‐cascade glucose depletion ROS scavenging regulate microenvironment. Specifically, AuPt@melanin‐incorporated (GHM3) dressing is developed facilitate efficient hyperthermia‐enhanced local scavenging. Mechanistically, vitro/vivo experiments RNA sequencing analysis demonstrate that GHM3 disrupts ROS‐inflammation cascade cycle downregulates ratio of M1/M2 macrophages, consequently improving outcomes dorsal skin wounds rats. In conclusion, this proposed offers facile, safe, highly treatment modality DFUs.

Язык: Английский

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Ultrasound-Augmented Multienzyme-like Nanozyme Hydrogel Spray for Promoting Diabetic Wound Healing

ACS Nano, Год журнала: 2023, Номер 17(16), С. 15962 - 15977

Опубликована: Авг. 3, 2023

Treatment of diabetic foot ulcers (DFU) needs to reduce inflammation, relieve hypoxia, lower blood glucose, promote angiogenesis, and eliminate pathogenic bacteria, but the therapeutic efficacy is greatly limited by diversity synergy drug functions as well DFU microenvironment itself. Herein, an ultrasound-augmented multienzyme-like nanozyme hydrogel spray was developed using hyaluronic acid encapsulated l-arginine ultrasmall gold nanoparticles Cu1.6O coloaded phosphorus doped graphitic carbon nitride nanosheets (ACPCAH). This possesses five types enzyme-like activities, including superoxide dismutase (SOD)-, catalase (CAT)-, glucose oxidase (GOx)-, peroxidase (POD)-, nitric oxide synthase (NOS)-like activities. The kinetics reaction mechanism sonodynamic/sonothermal synergistic enhancement SOD-CAT-GOx-POD/NOS cascade ACPCAH are fully investigated. Both in vitro vivo tests demonstrate that this can be activated thus accelerating wound healing effectively. study highlights a competitive approach based on nanozymes for development all-in-one therapies.

Язык: Английский

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Metal–Organic Frameworks and Their Composites for Chronic Wound Healing: From Bench to Bedside

Advanced Materials, Год журнала: 2023, Номер 36(2)

Опубликована: Авг. 1, 2023

Chronic wounds are characterized by delayed and dysregulated healing processes. As such, they have emerged as an increasingly significant threat. The associated morbidity socioeconomic toll clinically financially challenging, necessitating novel approaches in the management of chronic wounds. Metal-organic frameworks (MOFs) innovative type porous coordination polymers, with low toxicity high eco-friendliness. Documented anti-bacterial effects pro-angiogenic activity predestine these nanomaterials promising systems for treatment In this context, therapeutic applicability efficacy MOFs remain to be elucidated. It is, therefore, reviewed structural-functional properties their composite materials discusses how multifunctionality customizability can leveraged a clinical therapy

Язык: Английский

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A Hybrid Hydrogel with Intrinsic Immunomodulatory Functionality for Treating Multidrug-Resistant Pseudomonas aeruginosa Infected Diabetic Foot Ulcers

ACS Materials Letters, Год журнала: 2024, Номер 6(7), С. 2533 - 2547

Опубликована: Май 24, 2024

Contemporary options for multidrug-resistant bacteria infected diabetic foot ulcers (IDFUs) are predominantly nonspecific. These IDFU injuries often display prolonged inflammation and delayed tissue repair, mainly attributed to an overabundance of M1 macrophages in the hostile microenvironment. Although immunomodulatory hydrogels show promise IDFU-focused care, a targeted, safe transition from M2 using simplified techniques remains significant obstacle. Here, we introduce hybrid hydrogel (GGG) with inherent capabilities IDFUs. GGG is composed interpenetrating polymer networks featuring gallium-induced self-assembling glycyrrhizic acid photo-cross-linked gelatin methacryloyl matrix. Importantly, effectively decreases ratio conditions drug-resistant by disrupting iron metabolism scavenging reactive oxygen species, which contributes enhanced treatment results wounds. To sum up, strategy present provides straightforward, safe, highly effective therapeutic avenue managing

Язык: Английский

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Carbon dots as potential antioxidants for the scavenging of multi-reactive oxygen and nitrogen species

Chemical Engineering Journal, Год журнала: 2023, Номер 462, С. 142338 - 142338

Опубликована: Март 9, 2023

Язык: Английский

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An All‐in‐One “4A Hydrogel”: through First‐Aid Hemostatic, Antibacterial, Antioxidant, and Angiogenic to Promoting Infected Wound Healing

Small, Год журнала: 2023, Номер 19(27)

Опубликована: Март 28, 2023

Abstract Currently used wound dressings are ineffective. Hence, there is a need to develop introduce high‐performance medicament with multiple functions including rapid hemostasis and excellent antibacterial activity meet the growing worldwide demand for healing products. Here, inspired by strong adhesion of mussels enzyme‐mimicking nanometallic biomaterials, authors developed an injectable hydrogel overcome limitations current dressings. The synthesized via esterification reaction between poly(vinyl alcohol) (PVA) 3,4‐dihydroxyphenylalanine (DOPA), followed catechol‐metal coordination Cu 2+ catechol groups DOPA form PVA‐DOPA‐Cu (PDPC) hydrogel. PDPC possesses tissue adhesive, antioxidative, photothermal, antibacterial, hemostatic properties. rapidly efficiently stopped bleeding under different traumatic conditions, otherwise‐lethal liver injury, high‐pressure carotid artery rupture, even fatal cardiac penetration injuries in animal models. Furthermore, it demonstrated that affected repair regeneration accelerating re‐epithelialization, promoting collagen deposition, regulating inflammation, contributing vascularization. results show promising candidate hemorrhage control efficient clinical applications.

Язык: Английский

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Engineered Multifunctional Zinc–Organic Framework-Based Aggregation-Induced Emission Nanozyme for Accelerating Spinal Cord Injury Recovery

ACS Nano, Год журнала: 2024, Номер 18(3), С. 2355 - 2369

Опубликована: Янв. 10, 2024

Functional recovery following a spinal cord injury (SCI) is challenging. Traditional drug therapies focus on the suppression of immune responses; however, strategies for alleviating oxidative stress are lacking. Herein, we developed zinc–organic framework (Zn@MOF)-based aggregation-induced emission–active nanozymes accelerating SCI. A multifunctional Zn@MOF was modified with molecule 2-(4-azidobutyl)-6-(phenyl(4-(1,2,2-triphenylvinyl)phenyl)amino)-1H-phenalene-1,3-dione via bioorthogonal reaction, and resulting were denoted as Zn@MOF-TPD. These gradually released gallic acid zinc ions (Zn2+) at SCI site. The acid, scavenger reactive oxygen species (ROS), promoted antioxidation alleviated inflammation, re-establishing balance between ROS production antioxidant defense system. Zn2+ inhibited activity matrix metalloproteinase 9 (MMP-9) to facilitate regeneration neurons ROS-mediated NF-κB pathway secondary In addition, Zn@MOF-TPD protected myelin sheaths against trauma, glial scar formation, proliferation differentiation neural stem cells, thereby facilitating repair injured tissue promoting functional in rats contusive Altogether, this study suggests that possess potential stress-mediated pathophysiological damage motor

Язык: Английский

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MOF-derived bimetallic nanozyme to catalyze ROS scavenging for protection of myocardial injury

Theranostics, Год журнала: 2023, Номер 13(8), С. 2721 - 2733

Опубликована: Янв. 1, 2023

Rationale: Myocardial injury triggers intense oxidative stress, inflammatory response, and cytokine release, which are essential for myocardial repair remodeling.Excess reactive oxygen species (ROS) scavenging inflammation elimination have long been considered to reverse injuries.However, the efficacy of traditional treatments (antioxidant, anti-inflammatory drugs natural enzymes) is still poor due their intrinsic defects such as unfavorable pharmacokinetics bioavailability, low biological stability, potential side effects.Nanozyme represents a candidate effectively modulate redox homeostasis treatment ROS related diseases.Methods: We develop an integrated bimetallic nanozyme derived from metal-organic framework (MOF) eliminate alleviate inflammation.The (Cu-TCPP-Mn) synthesized by embedding manganese copper into porphyrin followed sonication, could mimic cascade activities superoxide dismutase (SOD) catalase (CAT) transform radicals hydrogen peroxide, catalysis peroxide water.Enzyme kinetic analysis oxygen-production velocities were performed evaluate enzymatic Cu-TCPP-Mn.We also established infarction (MI) ischemia-reperfusion (I/R) animal models verify anti-inflammation effect Cu-TCPP-Mn.Results: As demonstrated analysis, Cu-TCPP-Mn possesses good performance in both SOD-and CAT-like achieve synergistic provide protection injury.In MI I/R models, this promising reliable technology protect heart tissue stress inflammation-induced injury, enables function recover otherwise severe damage.Conclusions: This research provides facile applicable method MOF nanozyme, alternative injuries.

Язык: Английский

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Atomic-level design of metalloenzyme-like active pockets in metal–organic frameworks for bioinspired catalysis

Chemical Society Reviews, Год журнала: 2023, Номер 53(1), С. 137 - 162

Опубликована: Ноя. 29, 2023

Natural metalloenzymes with astonishing reaction activity and specificity underpin essential life transformations. Nevertheless, enzymes only operate under mild conditions to keep sophisticated structures active, limiting their potential applications. Artificial that recapitulate the catalytic of can not circumvent enzymatic fragility but also bring versatile functions into practice. Among them, metal-organic frameworks (MOFs) featuring diverse site-isolated metal sites supramolecular have emerged as promising candidates for move toward unparalleled properties behaviour enzymes. In this review, we systematically summarize significant advances in MOF-based metalloenzyme mimics a special emphasis on active pocket engineering at atomic level, including primary secondary coordination spheres. Then, deep understanding mechanisms advanced applications are discussed. Finally, perspective emerging frontier research is provided advance bioinspired catalysis.

Язык: Английский

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