Abstract
This
review
meticulously
examines
the
dynamics
of
river
microbiomes,
with
an
emphasis
on
Ganges
and
Yamuna
rivers
South
Asia.
These
are
vital
for
both
ecological
cultural
landscapes
offer
to
understand
interaction
between
anthropogenic
factors
their
impact
microbial
communities
activities.
Ecological
hydrological
such
as
seasonal
changes,
water
flow
physico‐chemical
properties
influence
diversity
abundance.
The
effect
heavy
metals
from
industrial
agricultural
sources
microbiome
how
these
pollutants
modify
community
structures
ecosystem
health
not
understood
well
yet.
underscores
need
sustainable
treatment
remediations
practical
engineering
solutions.
study
reveals
interactions,
whether
symbiotic
or
competitive,
affect
composition
functionality
riverine
communities.
An
innovative
aspect
our
research
is
potential
microbiomes
indicators
urban
sewage
contamination.
We
demonstrate
patterns
can
signal
pollution
levels,
proving
valuable
environmental
monitoring,
management
mitigation.
A
special
attention
role
microbes
in
ecosystems'
biogeochemical
cycles
has
been
paid
contribute
nutrient
recycling,
organic
matter
decomposition
overall
productivity,
underlining
crucial
maintaining
aesthetic
value
river.
Additionally,
evaluates
latest
methodologies
analysing
metagenomic
data,
including
functional
annotation
analysis
techniques.
Findings
highlight
key
importance
understanding
hydrology,
ecology
microbiology
researchers.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(6), С. 2711 - 2718
Опубликована: Фев. 1, 2024
Excessive
sulfite
usage
in
food
and
pharmaceutical
production
causes
respiratory
neurological
diseases,
underscoring
the
need
for
a
sensitive
rapid
quantification
strategy.
The
portable
sensing
platform
based
on
luminescent
hydrogel
sensor
is
powerful
tool
on-site,
real-time
detection
of
ions.
However,
lack
recyclability
almost
all
reaction-based
sensors
increases
application
cost.
This
study
constructed
reversible
upconversion
nanoprobe
combining
nanoparticles
(UCNPs)
pararosaniline
(PAR)
detection.
was
further
encapsulated
three-dimensional
polyacrylamide
matrix
to
create
background-free,
sensor.
near-infrared
excitation
UCNPs
avoids
autofluorescence
real
samples.
Meanwhile,
PAR
serves
as
specific
recognition
unit
After
addition
sulfites,
reaction
occurs
between
leading
recovery
characteristic
emission
at
540
nm,
achieving
Importantly,
this
under
thermal
treatment,
allowing
return
its
initial
state
thus
enabling
Furthermore,
developed
realize
point-of-care,
quantitative
proposed
provides
new
strategy
hazardous
substances
offers
insights
into
preparation
reversible,
highly
sensors.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(7), С. 4087 - 4087
Опубликована: Апрель 6, 2024
Cellular
survival
hinges
on
a
delicate
balance
between
accumulating
damages
and
repair
mechanisms.
In
this
intricate
equilibrium,
oxidants,
currently
considered
physiological
molecules,
can
compromise
vital
cellular
components,
ultimately
triggering
cell
death.
On
the
other
hand,
cells
possess
countermeasures,
such
as
autophagy,
which
degrades
recycles
damaged
molecules
organelles,
restoring
homeostasis.
Lysosomes
their
enzymatic
arsenal,
including
cathepsins,
play
critical
roles
in
balance,
influencing
cell’s
fate
toward
either
apoptosis
mechanisms
of
regulated
death
or
autophagy.
However,
interplay
reactive
oxygen
species
(ROS)
cathepsins
these
life-or-death
pathways
transcends
simple
cause-and-effect
relationship.
These
elements
directly
indirectly
influence
each
other’s
activities,
creating
complex
web
interactions.
This
review
delves
into
inner
workings
highlighting
pivotal
role
ROS
interplay.
ACS Applied Bio Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 1, 2024
Recent
advances
have
been
made
in
second
near-infrared
(NIR-II)
fluorescence
bioimaging
and
many
related
applications
because
of
its
advantages
deep
penetration,
high
resolution,
minimal
invasiveness,
good
dynamic
visualization.
To
achieve
high-performance
NIR-II
bioimaging,
various
materials
probes
with
bright
emission
extensively
explored
the
past
few
years.
Among
these
emissive
materials,
conjugated
polymers
small
molecules
attracted
wide
interest
due
to
their
native
biosafety
tunable
optical
performance.
This
review
summarizes
brightness
strategies
available
for
highlights
recent
developments
bioimaging.
A
concise,
detailed
overview
molecular
design
regulatory
approaches
is
provided
terms
brightness,
long
wavelengths,
superior
imaging
Then,
typical
cases
which
are
used
as
introduced
by
providing
step-by-step
examples.
Finally,
current
problems
challenges
associated
accessing
briefly
discussed,
significance
future
prospects
proposed
offer
helpful
guidance
development
materials.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Июнь 25, 2024
Abstract
Aberrantly
accumulated
metabolites
elicit
intra-
and
inter-cellular
pro-oncogenic
cascades,
yet
current
measurement
methods
require
sample
perturbation/disruption
lack
spatio-temporal
resolution,
limiting
our
ability
to
fully
characterize
their
function
distribution.
Here,
we
show
that
Raman
spectroscopy
(RS)
can
directly
detect
fumarate
in
living
cells
vivo
animal
tissues
ex
vivo,
RS
distinguish
between
Fumarate
hydratase
(Fh1)-deficient
Fh1-proficient
based
on
concentration.
Moreover,
reveals
the
spatial
compartmentalization
of
within
cellular
organelles
Fh1-deficient
cells:
consistent
with
disruptive
methods,
observe
highest
concentration
(37
±
19
mM)
mitochondria,
where
TCA
cycle
operates,
followed
by
cytoplasm
(24
13
then
nucleus
(9
6
mM).
Finally,
apply
from
an
inducible
mouse
model
FH
loss
kidney,
demonstrating
classify
status.
These
results
suggest
could
be
adopted
as
a
valuable
tool
for
small
molecule
metabolic
imaging,
enabling
situ
non-destructive
evaluation
compartmentalization.
Abstract
Cellular
senescence
is
a
steady
state
of
cell
cycle
arrest
necessary
to
maintain
homeostasis
in
organisms.
However,
senescent
cells
may
cause
neighboring
healthy
cells,
inducing
the
onset
several
diseases,
such
as
inflammation,
neurological
disorders,
and
atherosclerosis.
Therefore,
early
detection
cellular
extremely
important.
β‐Galactosidase
(β‐gal),
critical
marker
senescence,
can
be
monitored
facilitate
diagnosis
aging‐related
diseases.
Furthermore,
β‐gal
mainly
found
lysosomes,
which
have
pH
value
about
4.5–5.5.
Here,
we
developed
near‐infrared
fluorescent
probe
(QMOH‐Gal)
for
tracking
vitro
vivo
via
β‐gal.
In
addition,
displayed
high
sensitivity
specificity
with
good
fluorescence
signal
acidity
range.
Subsequently,
this
QMOH‐Gal
was
successfully
employed
differentiate
between
normal
by
monitoring
not
only
realized
zebrafish
but
also
palbociclib‐induced
breast
tumor
senescence.
Overall,
shows
great
promise
an
effective
tool
imaging
studying
biology
aging
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 9, 2025
Vanin-1
is
a
pantetheine
hydrolase
that
plays
key
role
in
inflammatory
diseases.
Effective
tools
for
noninvasive,
real-time
monitoring
of
are
lacking,
largely
due
to
background
fluorescence
interference
existing
probes.
To
address
this
issue,
we
developed
dual-modal
fluorescent
and
colorimetric
probe,
MB-Van1,
detect
with
high
sensitivity
selectivity.
MB-Van1
has
structure
optimized
exhibit
nearly
zero
fluorescence,
resulting
signal-to-noise
ratio
enables
the
accurate
detection
activity
various
biological
tissues.
In
vitro
experiments
demonstrated
had
limit
as
low
0.031
ng/mL
mode.
We
successfully
employ
observe
elevated
levels
tissues
mouse
models
rheumatoid
arthritis
(RA),
drug-induced
liver
injury
(DILI),
nonsteroidal
anti-inflammatory
drug
(NSAID)
enteropathy
models,
within
only
5
min.
This
advancement
provides
novel
approach
dynamic
changes
during
inflammation,
offering
new
strategies
early
diagnosis
therapeutic
assessment
other
related
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 22, 2025
Rheumatoid
arthritis
(RA)
is
a
destructive
autoimmune
disease
that
seriously
affects
human
health.
Due
to
the
lack
of
cure
for
RA,
good
prognosis
largely
depends
on
early
diagnosis
and
effective
treatment
monitoring
RA.
Therefore,
development
fluorescent
probes
capable
real-time
detection
RA
great
significance.
Dual-state
emission
(DSE)
molecules
can
emit
light
in
both
dilute
solution
solid
state,
making
them
ideal
fluorophores
constructing
probes.
However,
there
are
currently
no
reports
DSE
molecule-based
imaging.
Herein,
we
report
probe
MQP-Boc
based
novel
molecule
selectively
responds
viscosity
with
sensitive
fluorescence
changes
at
667
nm
mitochondria
targetable.
Cell
imaging
studies
show
detect
mitochondrial
perform
long-term
mitochondria,
which
significantly
superior
control
MQP-Ac.
Imaging
mouse
model
induced
by
λ-carrageenan
has
excellent
capabilities
Besides,
MQP-Boc,
significant
increases
joint
tissue
were
found
during
process.
All
results
indicate
an
new
tool
studying
diagnosing
