ABSTRACT
The
bacteria
living
in
the
human
gut
are
essential
for
host
health.
Though
composition
and
metabolism
of
these
well
described
both
healthy
hosts
those
with
intestinal
disease,
less
is
known
about
metabolic
activity
prior
to,
during,
disease
development—especially
regarding
bacterial
replication.
Here,
we
use
a
recently
developed
single-cell
technique
alongside
existing
metagenomics-based
tools
to
identify,
track,
quantify
replicating
ex
vivo
situ
dextran
sodium
sulfate
(DSS)
mouse
model
colitis.
We
show
that
proportion
decreases
when
mice
have
highest
levels
inflammation
returns
baseline
as
begin
recovering.
In
addition,
report
significant
alterations
community
during
colitis
development.
On
taxa
level,
observe
changes
abundance
such
mucus-degrading
Akkermansia
poorly
Erysipelatoclostridium
genus.
further
demonstrate
many
exhibit
variable
replication
rates
colitis,
including
muciniphila
.
Lastly,
development
positively
correlated
increases
presence
which
predicted
be
fast
replicators.
This
could
suggest
potential
replicate
quickly
may
an
advantage
inflammation.
These
data
support
need
additional
research
using
activity-based
approaches
characterize
response
its
consequences
microbial
community.
IMPORTANCE
It
inside
important
Indeed,
type
present
their
different
people
versus
disease.
However,
how
replicating,
especially
someone
begins
develop
particularly
it
thought
metabolically
active
more
relevant
address
this
gap
several
complementary
reveal
quickly,
recover
from
work
can
serve
future
identify
actively
growing
impacting
health,
or
why
particular
tend
thrive
Journal of Functional Foods,
Год журнала:
2024,
Номер
119, С. 106337 - 106337
Опубликована: Июль 6, 2024
Functional
foods
particularly
prebiotics
and
probiotics
have
attracted
a
lot
of
attention
due
to
their
ability
alter
gut
microbiota
an
impact
on
number
health
disease-related
factors,
in
relation
human
recent
years.
are
that
provide
benefits
beyond
simple
sustenance.
They
contain
biologically
active
ingredients
offer
additional
when
consumed
regular
basis
as
part
balanced
diet.
Prebiotics,
which
indigestible
fibers
specifically
promote
the
growth
activity
beneficial
bacteria,
probiotics,
live
microorganisms
upon
consumption
sufficient
amount,
shown
great
promise
modifying
composition
function
microbiota.
The
identified
review
problem
involves
clarifying
precise
impacts
immune
system
function,
composition,
metabolic
health,
disease
prevention.
This
emphasizes
numerous
advantages
preserving
balance
microbiota,
boosting
immunity,
enhancing
parameters,
reducing
risk
diseases.
also
insight
advances
formulation
functional
mitigating
conditions
synergy
between
prebiotics.
Metabolic
disease
is
a
worldwide
epidemic
that
has
become
public
health
problem.
Gut
microbiota
considered
to
be
one
of
the
important
factors
maintain
human
by
regulating
host
metabolism.
As
an
abundant
bacterium
in
gut,
Frontiers in Immunology,
Год журнала:
2023,
Номер
14
Опубликована: Июль 10, 2023
Arecoline
is
an
alkaloid
extracted
from
betel
nut,
which
has
various
pharmacological
effects.
In
the
present
study,
we
showed
that
arecoline
aggravated
experimental
acute
ulcerative
colitis
(UC)
induced
by
dextran
sodium
sulfate
(DSS)
in
mice.
We
measured
body
weight
and
colon
length,
evaluated
disease
activity
index,
pathology
sections,
levels
of
colonic
inflammatory
factors.
exacerbated
clinical
signs
UC
response
The
results
16S
rRNA
sequencing
fecal
samples
a
significant
decrease
percentage
probiotic
bacteria
Ligilactobacillus,
Limosilactobacillus
Lactobacillus
increase
conditionally
pathogenic
Odoribacter
Bacteroides
after
treatment.
Serum
untargeted
metabolomics
intervention
reduced
ergothioneine,
pentostatin,
diadenosine
tetraphosphate
other
metabolites
modulated
nicotinate
nicotinamide
metabolism,
metabolic
pathways,
glyoxylate
dicarboxylate
pathways
intestinal
microorganisms.
According
to
combined
microbial
metabolite
analysis,
influences
modulating
microbiota.
summary,
it
was
found
treatment
injury
responses
mice,
disrupted
host's
flora,
affected
changes
flora
metabolites,
thereby
exacerbating
development
inflammation.
Therefore,
consumption
nut
can
be
associated
with
risk
aggravating
UC.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(6), С. 3236 - 3236
Опубликована: Март 12, 2024
Ulcerative
colitis
(UC)
is
a
chronic
inflammatory
disorder
affecting
the
colon,
with
symptomatology
influenced
by
factors
including
environmental,
genomic,
microbial,
and
immunological
interactions.
Gut
microbiota
dysbiosis,
characterized
bacterial
population
alterations,
contributes
to
intestinal
homeostasis
disruption
aberrant
immune
system
activation,
thereby
exacerbating
state.
This
study
assesses
therapeutic
efficacy
of
intraperitoneal
(IP)
injected
flavonoids
(apigenin,
luteolin,
xanthohumol)
in
reduction
parameters
modulation
gut
murine
model
ulcerative
colitis.
Flavonoids
interact
modulating
their
composition
serving
as
substrates
for
fermentation
into
other
anti-inflammatory
bioactive
compounds.
Our
results
demonstrate
effectiveness
luteolin
xanthohumol
treatment
enhancing
relative
abundance
microorganisms,
attenuating
pro-inflammatory
species.
Moreover,
all
three
exhibit
cytokine
levels,
strongly
demonstrating
utility
alleviating
associated
physical
UC
symptoms.
suggests
that
this
molecule
potential
alternative
or
co-therapy
conventional
pharmacological
interventions,
potentially
mitigating
adverse
effects.
A
limited
impact
on
observed
apigenin,
attributed
its
solubility
constraints
via
chosen
administration
route,
resulting
accumulation
mesentery.
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(24), С. 13593 - 13609
Опубликована: Июнь 5, 2024
Inflammatory
bowel
disease
(IBD)
is
a
recurrent
inflammatory
condition
affecting
the
gastrointestinal
tract,
and
its
clinical
treatment
remains
suboptimal.
Probiotics
have
shown
effectiveness
in
alleviating
dextran
sulfate
sodium
salt
(DSS)-induced
colitis,
exhibiting
strain-specific
anti-inflammatory
properties.
In
this
study,
we
compared
therapeutic
effects
of
five
strains
Bifidobacterium
bifidum
isolated
from
healthy
adult
feces
on
DSS-induced
colitis
mice.
Additionally,
investigated
underlying
mechanisms
by
examining
gut
microbiota
composition
microbial
metabolome.
Our
findings
highlighted
superior
efficacy
B.
M1-3
to
other
strains.
It
significantly
improved
symptoms,
mitigated
barrier
disruption,
reduced
colonic
inflammation
DSS-treated
Moreover,
analysis
revealed
that
increased
abundance
diversity
microbiota.
Specifically,
it
Muribaculaceae,
Lactobacillus,
Bacteroides,
Enterorhabdus,
while
decreasing
Escherichia-Shigella.
Furthermore,
our
nontargeted
metabolomics
illustrated
had
regulatory
effect
various
metabolic
pathways,
including
tyrosine
metabolism,
lysine
degradation,
tryptophan
metabolism.
Importantly,
confirmed
efficiency
was
dependent
These
results
are
conducive
development
probiotic
products
for
colitis.
Comprehensive Reviews in Food Science and Food Safety,
Год журнала:
2024,
Номер
23(5)
Опубликована: Авг. 13, 2024
The
demand
for
functional
food
is
rising
in
tandem
with
the
prevalence
of
chronic
diseases.
Probiotics
play
a
crucial
role
development,
yet
their
ability
to
confer
health
benefits
host
remains
topic
debate
according
Food
and
Agriculture
Organization/World
Health
Organization
requirements.
application
culturomics,
innovative
isolation
techniques,
within
realm
probiotics
increasingly
deemed
essential
fully
harnessing
latent
potential
microbial
reservoirs.
Nevertheless,
its
confined
predominantly
human
fecal
sources.
Following
integration
probiogenomics,
significant
advancements
have
been
made
safety
assessment
probiotics.
However,
adoption
novel
probiotic
microorganisms
has
match
requisite
pace.
Progress
research
concerning
host-probiotic
interactions
by
employing
omics
technologies,
particularly
animal
models,
notable.
Nonetheless,
comprehensive
elucidation
mechanisms
action
trial
studies
are
lagging
behind.
Additionally,
viability
probiotics,
spanning
from
production
as
foods
transit
colon,
markedly
improved
through
encapsulation
techniques.
opportunities
exploration
persist
regarding
alternative
coating
materials
diverse
methodologies.
Furthermore,
there
discernible
transition
domain
probiotic-based
foods,
shifting
away
primarily
dairy-centric
focus
toward
inclusion
broader
array
categories.
This
review
addresses
critical
issues
ranging
sources
techniques
final
developments.
while
doing
so,
it
explores
probiogenomics
applications
characterization,
investigations
into
interactions,
strategies
stabilization
under
harsh
environmental
conditions.
