Urease-powered nanobots for radionuclide bladder cancer therapy

Nature Nanotechnology, Год журнала: 2024, Номер 19(4), С. 554 - 564

Опубликована: Янв. 15, 2024

Abstract Bladder cancer treatment via intravesical drug administration achieves reasonable survival rates but suffers from low therapeutic efficacy. To address the latter, self-propelled nanoparticles or nanobots have been proposed, taking advantage of their enhanced diffusion and mixing capabilities in urine when compared with conventional drugs passive nanoparticles. However, translational treating bladder are underexplored. Here, we tested radiolabelled mesoporous silica-based urease-powered an orthotopic mouse model cancer. In vivo ex results demonstrated nanobot accumulation at tumour site, eightfold increase revealed by positron emission tomography vivo. Label-free optical contrast based on polarization-dependent scattered light-sheet microscopy cleared bladders confirmed penetration Treating tumour-bearing mice intravesically administered radio-iodinated for radionuclide therapy resulted a size reduction about 90%, positioning as efficient delivery nanosystems therapy.

Язык: Английский

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Light-Triggered Mechanical Disruption of Extracellular Barriers by Swarms of Enzyme-Powered Nanomotors for Enhanced Delivery

ACS Nano, Год журнала: 2023, Номер 17(8), С. 7180 - 7193

Опубликована: Апрель 14, 2023

Targeted drug delivery depends on the ability of nanocarriers to reach target site, which requires penetration different biological barriers. Penetration is usually low and slow because passive diffusion steric hindrance. Nanomotors (NMs) have been suggested as next generation in due their autonomous motion associated mixing hydrodynamics, especially when acting collectively a swarm. Here, we explore concept enzyme-powered NMs designed such that they can exert disruptive mechanical forces upon laser irradiation. The urease-powered swarm behavior improve translational movement compared state-of-the-art nanocarriers, while optically triggered vapor nanobubbles destroy barriers reduce We show these motors, named Swarm 1, displace through microchannel blocked with type 1 collagen protein fibers (barrier model), accumulate onto fibers, disrupt them completely evaluate disruption microenvironment induced by (Swarm 1) quantifying efficiency second fluorescent 2) move cleared be taken up HeLa cells at other side channel. Experiments showed 2 clean path was increased 12-fold presence urea fuel no added. When dropped considerably only depicted 10-fold enhancement after pretreatment collagen-filled channel synergistic effect active (chemically propelled) (light-triggered nanobubbles) barrier represents clear advantage for improvement therapies currently fail inadequate passage carriers

Язык: Английский

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Toward Interdisciplinary Synergies in Molecular Communications: Perspectives from Synthetic Biology, Nanotechnology, Communications Engineering and Philosophy of Science

Life, Год журнала: 2023, Номер 13(1), С. 208 - 208

Опубликована: Янв. 11, 2023

Within many chemical and biological systems, both synthetic natural, communication via messengers is widely viewed as a key feature. Often known molecular communication, such has been concern in the fields of biologists, nanotechnologists, communications engineers, philosophers science. However, interactions between these are currently limited. Nevertheless, fact that same basic phenomenon studied by all raises question whether there unexploited interdisciplinary synergies. In this paper, we summarize perspectives each field on communications, highlight potential synergies, discuss ongoing challenges to exploit present future for efforts area.

Язык: Английский

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Swarms of Enzyme‐Powered Nanomotors Enhance the Diffusion of Macromolecules in Viscous Media

Small, Год журнала: 2024, Номер 20(11)

Опубликована: Янв. 10, 2024

Abstract Over the past decades, development of nanoparticles (NPs) to increase efficiency clinical treatments has been subject intense research. Yet, most NPs have reported possess low efficacy as their actuation is hindered by biological barriers. For instance, synovial fluid (SF) present in joints mainly composed hyaluronic acid (HA). These viscous media pose a challenge for many applications nanomedicine, passive tend become trapped complex networks, which reduces ability reach target location. This problem can be addressed using active (nanomotors, NMs) that are self‐propelled enzymatic reactions, although enzyme‐powered NMs, capable navigating these environments, remains considerable challenge. Here, synergistic effects two NMs troops, namely hyaluronidase (HyaNMs, Troop 1) and urease (UrNMs, 2) demonstrated. 1 interacts with SF reducing its viscosity, thus allowing 2 swim more easily through SF. Through collective motion, increases diffusion macromolecules. results pave way widespread use e.g., treating joint injuries improving therapeutic effectiveness compared traditional methods.

Язык: Английский

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Motility of an autonomous protein-based artificial motor that operates via a burnt-bridge principle

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Фев. 23, 2024

Abstract Inspired by biology, great progress has been made in creating artificial molecular motors. However, the dream of harnessing proteins – building blocks selected nature to design autonomous motors so far remained elusive. Here we report synthesis and characterization Lawnmower, an autonomous, protein-based motor comprised a spherical hub decorated with proteases. Its “burnt-bridge” motion is directed cleavage peptide lawn, promoting towards unvisited substrate. We find that Lawnmowers exhibit directional average speeds up 80 nm/s, comparable biological By selectively patterning lawn on microfabricated tracks, furthermore show Lawnmower capable track-guided motion. Our work opens avenue nanotechnology applications protein

Язык: Английский

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Catalase-Powered Nanobots for Overcoming the Mucus Barrier

ACS Nano, Год журнала: 2024, Номер 18(26), С. 16701 - 16714

Опубликована: Июнь 17, 2024

Biological barriers present a significant obstacle to treatment, especially when drugs are administered locally increase their concentrations at the target site while minimizing unintended off-target effects. Among these barriers, mucus presents challenge, as it serves protective layer in respiratory, urogenital, and gastrointestinal tracts. Its role is shield underlying epithelial cells from pathogens toxic compounds but also impedes efficient delivery of drugs. Despite exploration mucolytic agents improve drug delivery, overcoming this barrier remains hurdle. In our study, we investigate an alternative approach involving use catalase-powered nanobots. We vitro model that simulates intestinal secretion demonstrate dual functionality This includes ability disrupt mucus, which confirmed through ex vivo validation, well self-propulsion overcome barrier, resulting 60-fold compared with passive nanoparticles. Therefore, findings highlight potential utility nanobots carriers for therapeutic since they could enhance efficiency by penetrating barrier.

Язык: Английский

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Magnetic micromotors crossing lipid membranes

Nanoscale, Год журнала: 2024, Номер 16(5), С. 2432 - 2443

Опубликована: Янв. 1, 2024

Nano/micromotors are self-propelled particles that show enhanced motion upon being triggered by a stimulus.

Язык: Английский

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Chemotactic Interactions Drive Migration of Membraneless Active Droplets

Journal of the American Chemical Society, Год журнала: 2024, Номер 146(23), С. 15965 - 15976

Опубликована: Апрель 15, 2024

In nature, chemotactic interactions are ubiquitous and play a critical role in driving the collective behavior of living organisms. Reproducing these vitro is still paramount challenge due to complexity mimicking controlling cellular features, such as tangled metabolic networks, cytosolic macromolecular crowding, migration, on microorganism size scale. Here, we generate enzymatically active cell-sized droplets able move freely, by following chemical gradient, interact with surrounding manner. The enzyme within generates pH gradient that extends outside edge droplets. We discovered external triggers droplet migration controls its directionality, which selectively toward neighboring Hence, changing activity inside droplet, tuned speed. Furthermore, showed cellular-like features can facilitate reconstitution simple linear protometabolic pathway increase final reaction product generation. Our work suggests stable membraneless reproduce complex biological phenomena, opening new perspectives bioinspired materials synthetic biology tools.

Язык: Английский

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Motion control of chemically powered colloidal motors

Advances in Colloid and Interface Science, Год журнала: 2025, Номер 341, С. 103475 - 103475

Опубликована: Март 15, 2025

Язык: Английский

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Urease-Powered Black TiO₂ Micromotors for Photothermal Therapy of Bladder Cancer

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(3), С. 3019 - 3030

Опубликована: Янв. 13, 2024

Urease-powered nano/micromotors can move at physiological urea concentrations, making them useful for biomedical applications, such as treating bladder cancer. However, their movement in biological environments is still challenging. Herein, Janus micromotors based on black TiO2 with urease asymmetric catalytic coating were designed to take benefit of the optical properties under near-infrared light and capability simulated (urea). The microspheres half-coated a thin layer Au, l-Cysteine was utilized attach enzyme Au surface using its thiol group. Biocatalytic hydrolysis through biologically relevant concentrations provided driving force micromotors. A variety parameters, fuel concentration, viscosity, ionic character environment, used investigate how moved different water, PBS, NaCl, urine. results indicate that are propelled self-diffusiophoresis caused by enzymatic catalysis. Due low toxicity vitro anticancer effect, effective agents photothermal therapy, which help kill cancer cells. These promising suggest biocompatible hold great potential improving treatment facilitating diagnosis.

Язык: Английский

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