Transport of Nanoparticles into Plants and Their Detection Methods

Nanomaterials, Год журнала: 2024, Номер 14(2), С. 131 - 131

Опубликована: Янв. 5, 2024

Nanoparticle transport into plants is an evolving field of research with diverse applications in agriculture and biotechnology. This article provides overview the challenges prospects associated nanoparticles plants, focusing on delivery methods detection within plant tissues. Passive assisted methods, including use roots leaves as introduction sites, are discussed, along their respective advantages limitations. The barriers encountered nanoparticle to highlighted, emphasizing need for innovative approaches (e.g., stem a new recognition site) optimize efficiency. In recent years, efforts have intensified, leading evendeeper understanding intricate mechanisms governing interaction nanomaterials tissues cells. Investigations uptake pathways translocation revealed nuanced responses different types nanoparticles. Additionally, this delves importance studying localization quantification Various techniques presented valuable tools comprehensively nanoparticle–plant interactions. reliance multiple data validation emphasized enhance reliability findings. future outlooks explored, potential alternative such stems, continued development formulations that improve adhesion penetration. By addressing these fostering multidisciplinary research, poised make significant contributions sustainable environmental management.

Язык: Английский

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Thermo-Optoplasmonic Single-Molecule Sensing on Optical Microcavities

ACS Nano, Год журнала: 2024, Номер 18(27), С. 17534 - 17546

Опубликована: Июнь 26, 2024

Whispering-gallery-mode (WGM) resonators are powerful instruments for single-molecule sensing in biological and biochemical investigations. WGM sensors leveraged by plasmonic nanostructures, known as optoplasmonic sensors, provide sensitivity down to single atomic ions. In this article, we describe that the response of upon attachment protein molecules strongly depends on intensity WGM. At low intensity, binding causes red shifts resonance wavelengths, reactive mechanism. By contrast, blue obtained at high intensities, which explain thermo-optoplasmonic (TOP) sensing, where transform absorbed radiation into heat. To support our conclusions, experimentally investigated seven complexes; observed dye molecules, amino acids, anomalous absorption enzymes near-infrared spectral region. As an example application, propose a physical model TOP can be used development spectrometers.

Язык: Английский

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Single-molecule FRET for probing nanoscale biomolecular dynamics

Nature Reviews Physics, Год журнала: 2024, Номер unknown

Опубликована: Сен. 12, 2024

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Ultraviolet Nanophotonics Enables Autofluorescence Correlation Spectroscopy on Label-Free Proteins with a Single Tryptophan

Nano Letters, Год журнала: 2023, Номер 23(2), С. 497 - 504

Опубликована: Янв. 5, 2023

Using the ultraviolet autofluorescence of tryptophan amino acids offers fascinating perspectives to study single proteins without drawbacks fluorescence labeling. However, low signals have so far limited UV detection large containing several tens residues. This limit is not compatible with vast majority which contain only a few tryptophans. Here we push sensitivity label-free correlation spectroscopy (UV-FCS) down level. Our results show how combination nanophotonic plasmonic antennas, antioxidants, and background reduction techniques can improve signal-to-background ratio by over an order magnitude enable UV-FCS on thermonuclease residue. breakthrough unlocks applicability technique broad library proteins.

Язык: Английский

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3D Super-Resolution Fluorescence Imaging of Microgels

Annual Review of Physical Chemistry, Год журнала: 2023, Номер 74(1), С. 391 - 414

Опубликована: Фев. 8, 2023

Super-resolution fluorescence microscopy techniques are powerful tools to investigate polymer systems. In this review, we address how these have been applied hydrogel nano- and microparticles, so-called or microgels. We outline which research questions on microgels could be addressed what new insights achieved. Studies of the morphology, shape, deformation microgels; their internal compartmentalization; cross-linker distribution polarity inside them; dynamics diffusion summarized. particular, abilities super-resolve structures in three dimensions boosted field also allowed researchers obtain impressive 3D images deformed Accessing information beyond localization, such as spectral lifetime properties correlative imaging combination data with other methods, shines light onto systems helps us understand complexity detail. Such future trends developments addressed.

Язык: Английский

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Fluorescence-Detected Two-Dimensional Electronic Spectroscopy of a Single Molecule

Nano Letters, Год журнала: 2024, Номер unknown

Опубликована: Сен. 27, 2024

Single-molecule fluorescence spectroscopy is a powerful method that avoids ensemble averaging, but its temporal resolution limited by the lifetime to nanoseconds at most. At level, two-dimensional provides insight into ultrafast femtosecond processes, such as energy transfer and line broadening, even beyond Fourier limit, correlating pump probe spectra. Here, we combine these two techniques demonstrate coherent 2D of individual dibenzoterrylene (DBT) molecules room temperature. We excite molecule in confocal microscope with phase-modulated train pulses detect emitted single-photon counting detectors. Using phase-sensitive detection scheme, were able measure nonlinear spectra most DBT studied. Our applicable wide range single emitters opens new avenues for understanding quantum objects on time scales.

Язык: Английский

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Thiolation for Enhancing Photostability of Fluorophores at the Single‐Molecule Level

Angewandte Chemie International Edition, Год журнала: 2023, Номер 63(1)

Опубликована: Ноя. 17, 2023

Fluorescent probes are essential for single-molecule imaging. However, their application in biological systems is often limited by the short photobleaching lifetime. To overcome this, we developed a novel thiolation strategy squaraine dyes. By introducing of central cyclobutene (thio-squaraine), observed ≈5-fold increase Our data analysis attributes this improvement to improved photostability resulting from thiolation. Interestingly, bulk measurements show rapid oxidation thio-squaraine its oxo-analogue under irradiation, giving perception inferior photostability. This discrepancy between and environments can be ascribed factors latter, including larger intermolecular distances restricted mobility, which reduce interactions fluorophore reactive oxygen species produced other fluorophores, ultimately impacting photoconversion rate. We demonstrate remarkable performance various imaging buffers, such as glucose oxidase with catalase (GLOX) GLOX+trolox. successfully employed these photostable tracking CD56 membrane protein monitoring mitochondria movements live neurons. revealed distinct motion states corresponding fractions. investigation expected propel development probes, particularly scenarios where suboptimal performance.

Язык: Английский

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Future Paths in Cryogenic Single-Molecule Fluorescence Spectroscopy

The Journal of Physical Chemistry C, Год журнала: 2023, Номер 128(1), С. 3 - 18

Опубликована: Дек. 22, 2023

In the last three decades, cryogenic single-molecule fluorescence spectroscopy has provided average-free understanding of photophysics and fundamental interactions at molecular scales. Furthermore, they propose original pathways applications in treatment storage quantum information. The ultranarrow lifetime-limited zero-phonon line acts as an excellent sensor to local perturbations caused either by intrinsic dynamical degrees freedom, or external perturbations, such those electric fields, elastic acoustic deformations, light-induced dynamics. Single aromatic hydrocarbon molecules, being sensitive nanoscale probing nanometer scales, are potential miniaturized platforms for integrated photonics. this Perspective, we look back some past advances optical microscopy perspectives future development.

Язык: Английский

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Photothermal Microscopy and Spectroscopy with Nanomechanical Resonators

The Journal of Physical Chemistry C, Год журнала: 2023, Номер 127(45), С. 21915 - 21929

Опубликована: Ноя. 6, 2023

In nanomechanical photothermal absorption spectroscopy and microscopy, the measured substance becomes a part of detection system itself, inducing resonance frequency shift upon thermal relaxation. Suspended, nanometer-thin ceramic or 2D material resonators are innately highly sensitive detectors localized heat exchanges from substances on their surface integrated into resonator itself. Consequently, combined nanoresonator-analyte is self-measuring spectrometer microscope responding to substance's transfer over entire spectrum for which it absorbs, according intensity experiences. Limited by own thermostatistical fluctuation phenomena, nanoresonators have demonstrated sufficient sensitivity measuring trace analyte as well single particles molecules with incoherent light focused wide-field coherent light. They versatile in design, support various sampling methods─potentially including hydrated sample encapsulation─and hyphenation other spectroscopic methods, capable wide range applications fingerprinting, separation science, sciences.

Язык: Английский

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An optofluidic antenna for enhancing the sensitivity of single-emitter measurements

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Март 21, 2024

Abstract Many single-molecule investigations are performed in fluidic environments, for example, to avoid unwanted consequences of contact with surfaces. Diffusion molecules this arrangement limits the observation time and number collected photons, thus, compromising studies processes fast or slow dynamics. Here, we introduce a planar optofluidic antenna (OFA), which enhances fluorescence signal from by about 5 times per passage, leads 7-fold more frequent returns volume, significantly lengthens diffusion within one passage. We use multi-parameter detection (sm-MFD), correlation spectroscopy (FCS) Förster resonance energy transfer (FRET) measurements characterize our OFAs. The advantages showcased examining both (ms) (50 μ s) dynamics DNA four-way (Holliday) junctions real-time resolution. FRET trajectories provide evidence absence an intermediate conformational state upper bound its lifetime. ease implementation compatibility various microscopy modalities make OFAs broadly applicable diverse range studies.

Язык: Английский

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