Efficient, nonparametric removal of noise and recovery of probability distributions from time series using nonlinear-correlation functions: Photon and photon-counting noise DOI
Mainak Dhar, Mark A. Berg

The Journal of Chemical Physics, Journal Year: 2024, Volume and Issue: 161(3)

Published: July 19, 2024

A preceding paper [M. Dhar, J. A. Dickinson, and M. Berg, Chem. Phys. 159, 054110 (2023)] shows how to remove additive noise from an experimental time series, allowing both the equilibrium distribution of system its Green’s function be recovered. The approach is based on nonlinear-correlation functions fully nonparametric: no initial model or needed. However, single-molecule spectroscopy often produces series with either photon photon-counting noise. Unlike noise, signal-size correlated quantized. Photon counting adds potential for bias. This extends noise-corrected-correlation methods these cases tests them synthetic datasets. Neither correlation nor quantization a significant complication. Analysis sampling error yields guidelines data quality needed recover properties given complexity. We show that bias in can corrected, even at high count rates optimize resolution. Using all results, we discuss factors limit resolution conditions would push measurements into submicrosecond region.

Language: Английский

Transport of Nanoparticles into Plants and Their Detection Methods DOI Creative Commons
Anca Awal Sembada, I. Wuled Lenggoro

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(2), P. 131 - 131

Published: Jan. 5, 2024

Nanoparticle transport into plants is an evolving field of research with diverse applications in agriculture and biotechnology. This article provides overview the challenges prospects associated nanoparticles plants, focusing on delivery methods detection within plant tissues. Passive assisted methods, including use roots leaves as introduction sites, are discussed, along their respective advantages limitations. The barriers encountered nanoparticle to highlighted, emphasizing need for innovative approaches (e.g., stem a new recognition site) optimize efficiency. In recent years, efforts have intensified, leading evendeeper understanding intricate mechanisms governing interaction nanomaterials tissues cells. Investigations uptake pathways translocation revealed nuanced responses different types nanoparticles. Additionally, this delves importance studying localization quantification Various techniques presented valuable tools comprehensively nanoparticle–plant interactions. reliance multiple data validation emphasized enhance reliability findings. future outlooks explored, potential alternative such stems, continued development formulations that improve adhesion penetration. By addressing these fostering multidisciplinary research, poised make significant contributions sustainable environmental management.

Language: Английский

Citations

50
Ultraviolet Nanophotonics Enables Autofluorescence Correlation Spectroscopy on Label-Free Proteins with a Single Tryptophan DOI
Prithu Roy, Jean-Benoît Claude, Sunny Tiwari

et al.

Nano Letters, Journal Year: 2023, Volume and Issue: 23(2), P. 497 - 504

Published: Jan. 5, 2023

Using the ultraviolet autofluorescence of tryptophan amino acids offers fascinating perspectives to study single proteins without drawbacks fluorescence labeling. However, low signals have so far limited UV detection large containing several tens residues. This limit is not compatible with vast majority which contain only a few tryptophans. Here we push sensitivity label-free correlation spectroscopy (UV-FCS) down level. Our results show how combination nanophotonic plasmonic antennas, antioxidants, and background reduction techniques can improve signal-to-background ratio by over an order magnitude enable UV-FCS on thermonuclease residue. breakthrough unlocks applicability technique broad library proteins.

Language: Английский

Citations

17
Thermo-Optoplasmonic Single-Molecule Sensing on Optical Microcavities DOI Creative Commons
N. A. Toropov, Matthew C. Houghton, Deshui Yu

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(27), P. 17534 - 17546

Published: June 26, 2024

Whispering-gallery-mode (WGM) resonators are powerful instruments for single-molecule sensing in biological and biochemical investigations. WGM sensors leveraged by plasmonic nanostructures, known as optoplasmonic sensors, provide sensitivity down to single atomic ions. In this article, we describe that the response of upon attachment protein molecules strongly depends on intensity WGM. At low intensity, binding causes red shifts resonance wavelengths, reactive mechanism. By contrast, blue obtained at high intensities, which explain thermo-optoplasmonic (TOP) sensing, where transform absorbed radiation into heat. To support our conclusions, experimentally investigated seven complexes; observed dye molecules, amino acids, anomalous absorption enzymes near-infrared spectral region. As an example application, propose a physical model TOP can be used development spectrometers.

Language: Английский

Citations

8
Single-molecule FRET for probing nanoscale biomolecular dynamics DOI
Daniel Nettels, Nicola Galvanetto, Miloš T. Ivanović

et al.

Nature Reviews Physics, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 12, 2024

Citations

7
Fluorescence-Detected Two-Dimensional Electronic Spectroscopy of a Single Molecule DOI

Sanchayeeta Jana,

Simon Durst,

Markus Lippitz

et al.

Nano Letters, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 27, 2024

Single-molecule fluorescence spectroscopy is a powerful method that avoids ensemble averaging, but its temporal resolution limited by the lifetime to nanoseconds at most. At level, two-dimensional provides insight into ultrafast femtosecond processes, such as energy transfer and line broadening, even beyond Fourier limit, correlating pump probe spectra. Here, we combine these two techniques demonstrate coherent 2D of individual dibenzoterrylene (DBT) molecules room temperature. We excite molecule in confocal microscope with phase-modulated train pulses detect emitted single-photon counting detectors. Using phase-sensitive detection scheme, were able measure nonlinear spectra most DBT studied. Our applicable wide range single emitters opens new avenues for understanding quantum objects on time scales.

Language: Английский

Citations

5
3D Super-Resolution Fluorescence Imaging of Microgels DOI Creative Commons
Oleksii Nevskyi, Dominik Wöll

Annual Review of Physical Chemistry, Journal Year: 2023, Volume and Issue: 74(1), P. 391 - 414

Published: Feb. 8, 2023

Super-resolution fluorescence microscopy techniques are powerful tools to investigate polymer systems. In this review, we address how these have been applied hydrogel nano- and microparticles, so-called or microgels. We outline which research questions on microgels could be addressed what new insights achieved. Studies of the morphology, shape, deformation microgels; their internal compartmentalization; cross-linker distribution polarity inside them; dynamics diffusion summarized. particular, abilities super-resolve structures in three dimensions boosted field also allowed researchers obtain impressive 3D images deformed Accessing information beyond localization, such as spectral lifetime properties correlative imaging combination data with other methods, shines light onto systems helps us understand complexity detail. Such future trends developments addressed.

Language: Английский

Citations

12
Thiolation for Enhancing Photostability of Fluorophores at the Single‐Molecule Level DOI
Jinyang Liu, Bingjie Zhao, Xuebo Zhang

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 63(1)

Published: Nov. 17, 2023

Fluorescent probes are essential for single-molecule imaging. However, their application in biological systems is often limited by the short photobleaching lifetime. To overcome this, we developed a novel thiolation strategy squaraine dyes. By introducing of central cyclobutene (thio-squaraine), observed ≈5-fold increase Our data analysis attributes this improvement to improved photostability resulting from thiolation. Interestingly, bulk measurements show rapid oxidation thio-squaraine its oxo-analogue under irradiation, giving perception inferior photostability. This discrepancy between and environments can be ascribed factors latter, including larger intermolecular distances restricted mobility, which reduce interactions fluorophore reactive oxygen species produced other fluorophores, ultimately impacting photoconversion rate. We demonstrate remarkable performance various imaging buffers, such as glucose oxidase with catalase (GLOX) GLOX+trolox. successfully employed these photostable tracking CD56 membrane protein monitoring mitochondria movements live neurons. revealed distinct motion states corresponding fractions. investigation expected propel development probes, particularly scenarios where suboptimal performance.

Language: Английский

Citations

11
Interferometric scattering microscopy DOI
Naomi S. Ginsberg, Chia‐Lung Hsieh, Philipp Kukura

et al.

Nature Reviews Methods Primers, Journal Year: 2025, Volume and Issue: 5(1)

Published: April 10, 2025

Citations

0
Overcoming experimental obstacles in two-dimensional spectroscopy of a single molecule DOI Creative Commons

Sanchayeeta Jana,

Simon Durst,

Lucas Ludwig

et al.

The Journal of Chemical Physics, Journal Year: 2025, Volume and Issue: 162(18)

Published: May 9, 2025

Two-dimensional electronic spectroscopy provides information on coupling and energy transfer between excited states ultrafast timescales. Only recently, incoherent fluorescence detection has made it possible to combine this method with single-molecule optical reach the ultimate limit of sensitivity. The main obstacle been low number photons detected due limited photostability. Here, we discuss key experimental choices that allowed us overcome these obstacles: broadband acousto-optic modulation, accurate phase-locked loops, photon-counting lock-in detection, delay stage linearization, detector dead-time compensation. We demonstrate how acquired photon stream data can be used post-select events according specific criteria.

Language: Английский

Citations

0
Small but large: Single organic molecules as hybrid platforms for quantum technologies DOI Creative Commons
Burak Gurlek, Daqing Wang

Physical Review Research, Journal Year: 2025, Volume and Issue: 7(2)

Published: May 22, 2025

Single organic molecules embedded in solid-state matrices exhibit remarkable optical properties, making them competitive candidates for single-photon sources and quantum nonlinear elements. However, the lack of long-lived internal states imposes significant constraints on their application broader field technologies. In this article, we reexamine single-molecule host-guest system from first principles, elaborate rich encompasses, put forward strategies to harness applications memory, spin-photon interface, spin register, optomechanics. Further, discuss potential leveraging vast chemical space highlight challenges opportunities molecular systems along these directions. Published by American Physical Society 2025

Language: Английский

Citations

0