Glyphosate perturbs the gut microbiota of honey bees

Proceedings of the National Academy of Sciences, Год журнала: 2018, Номер 115(41), С. 10305 - 10310

Опубликована: Сен. 24, 2018

Glyphosate, the primary herbicide used globally for weed control, targets 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme in shikimate pathway found plants and some microorganisms. Thus, glyphosate may affect bacterial symbionts of animals living near agricultural sites, including pollinators such as bees. The honey bee gut microbiota is dominated by eight species that promote weight gain reduce pathogen susceptibility. gene encoding EPSPS present almost all sequenced genomes bacteria, indicating they are potentially susceptible to glyphosate. We demonstrated relative absolute abundances dominant decreased bees exposed at concentrations documented environment. Glyphosate exposure young workers increased mortality subsequently opportunistic Serratia marcescens Members varied susceptibility glyphosate, largely corresponding whether possessed an class I (sensitive glyphosate) or II (insensitive glyphosate). This basis differences sensitivity was confirmed using vitro experiments which from bacteria cloned into Escherichia coli All strains core species, Snodgrassella alvi, encode a sensitive EPSPS, reduction S. alvi levels consistent experimental result. However, appear possess alternative mechanism resistance. can perturb their beneficial microbiota, affecting health effectiveness pollinators.

Язык: Английский

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Antibiotic exposure perturbs the gut microbiota and elevates mortality in honeybees

PLoS Biology, Год журнала: 2017, Номер 15(3), С. e2001861 - e2001861

Опубликована: Март 14, 2017

Gut microbiomes play crucial roles in animal health, and shifts the gut microbial community structure can have detrimental impacts on hosts. Studies with vertebrate models human subjects suggest that antibiotic treatments greatly perturb native community, thereby facilitating proliferation of pathogens. In fact, persistent infections following treatment are a major medical issue. apiculture, antibiotics frequently used to prevent bacterial larval bees, but impact antibiotic-induced dysbiosis (microbial imbalance) bee health susceptibility disease has not been fully elucidated. Here, we evaluated effects exposure size composition honeybee communities. We monitored survivorship bees order determine if microbiome performed experiments whether increases infection by opportunistic Our results show both microbiome. Antibiotic resulted decreased survivorship, hive laboratory which were exposed Together, these resulting from affects part due increased ubiquitous Not only do our highlight importance they also provide insights into how communities host health.

Язык: Английский

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The role of the gut microbiome in health and disease of adult honey bee workers

Current Opinion in Insect Science, Год журнала: 2018, Номер 26, С. 97 - 104

Опубликована: Фев. 8, 2018

Язык: Английский

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Immune system stimulation by the native gut microbiota of honey bees

Royal Society Open Science, Год журнала: 2017, Номер 4(2), С. 170003 - 170003

Опубликована: Фев. 1, 2017

Gut microbial communities can greatly affect host health by modulating the host's immune system. For many important insects, however, relationship between gut microbiota and function remains poorly understood. Here, we test whether symbionts of honey bee induce expression antimicrobial peptides (AMPs), a crucial component insect innate immunity. We find that bees up-regulate gene AMPs apidaecin hymenoptaecin in tissue when is present. Using targeted proteomics, detected both lumen haemolymph; higher concentrations were found harbouring normal than lacking microbiota. In vitro assays, cultured strains showed variable susceptibility to AMPs, although seem possess elevated resistance compared Escherichia coli . some trials, colonization resulted improved survivorship following injection with E. Our results show native, non-pathogenic flora induces responses host. Such might be mechanism regulate microbiota, could potentially benefit priming system against future pathogenic infections.

Язык: Английский

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Disentangling metabolic functions of bacteria in the honey bee gut

PLoS Biology, Год журнала: 2017, Номер 15(12), С. e2003467 - e2003467

Опубликована: Дек. 12, 2017

It is presently unclear how much individual community members contribute to the overall metabolic output of a gut microbiota. To address this question, we used honey bee, which harbors relatively simple and remarkably conserved microbiota with striking parallels mammalian system importance for bee health. Using untargeted metabolomics, profiled changes in gnotobiotic bees that were colonized complete reconstituted from cultured strains. We then determined contribution mono-colonized recapitulated our findings using vitro cultures. Our results show utilizes wide range pollen-derived substrates, including flavonoids outer pollen wall components, suggesting key role degradation recalcitrant secondary plant metabolites digestion. In turn, multiple species responsible accumulation organic acids aromatic compound intermediates. Moreover, specific symbiont, Bifidobacterium asteroides, stimulated production host hormones known impact development. While found evidence cross-feeding interactions, approximately 80% identified also observed bees, Lactobacilli being largest share output. These that, despite prolonged evolutionary associations, bacteria can independently establish metabolize compounds gut. study reveals diverse bacterial functions are likely health provide fundamental insights into activities partitioned within communities.

Язык: Английский

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Honey bees as models for gut microbiota research

Lab Animal, Год журнала: 2018, Номер 47(11), С. 317 - 325

Опубликована: Окт. 11, 2018

Язык: Английский

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Simple animal models for microbiome research

Nature Reviews Microbiology, Год журнала: 2019, Номер 17(12), С. 764 - 775

Опубликована: Авг. 15, 2019

Язык: Английский

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Functional roles and metabolic niches in the honey bee gut microbiota

Current Opinion in Microbiology, Год журнала: 2018, Номер 43, С. 69 - 76

Опубликована: Янв. 12, 2018

Язык: Английский

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Microbial transmission in animal social networks and the social microbiome

Nature Ecology & Evolution, Год журнала: 2020, Номер 4(8), С. 1020 - 1035

Опубликована: Июнь 22, 2020

Язык: Английский

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Early-life disruption of amphibian microbiota decreases later-life resistance to parasites

Nature Communications, Год журнала: 2017, Номер 8(1)

Опубликована: Июль 13, 2017

Abstract Changes in the early-life microbiota of hosts might affect infectious disease risk throughout life, if such disruptions during formative times alter immune system development. Here, we test whether an disruption host-associated affects later-life resistance to infections by manipulating tadpoles and challenging them with parasitic gut worms as adults. We find that tadpole bacterial diversity is negatively correlated parasite establishment adult frogs: frogs had reduced have three more than adults without their manipulated tadpoles. In contrast, exposure not frogs. Thus, this experimental setup, has lasting reductions on host infections, which possibly mediated its effects Our results support idea preventing confer protection against diseases later life.

Язык: Английский

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