Glyphosate perturbs the gut microbiota of honey bees

Proceedings of the National Academy of Sciences, Год журнала: 2018, Номер 115(41), С. 10305 - 10310

Опубликована: Сен. 24, 2018

Glyphosate, the primary herbicide used globally for weed control, targets 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme in shikimate pathway found plants and some microorganisms. Thus, glyphosate may affect bacterial symbionts of animals living near agricultural sites, including pollinators such as bees. The honey bee gut microbiota is dominated by eight species that promote weight gain reduce pathogen susceptibility. gene encoding EPSPS present almost all sequenced genomes bacteria, indicating they are potentially susceptible to glyphosate. We demonstrated relative absolute abundances dominant decreased bees exposed at concentrations documented environment. Glyphosate exposure young workers increased mortality subsequently opportunistic Serratia marcescens Members varied susceptibility glyphosate, largely corresponding whether possessed an class I (sensitive glyphosate) or II (insensitive glyphosate). This basis differences sensitivity was confirmed using vitro experiments which from bacteria cloned into Escherichia coli All strains core species, Snodgrassella alvi, encode a sensitive EPSPS, reduction S. alvi levels consistent experimental result. However, appear possess alternative mechanism resistance. can perturb their beneficial microbiota, affecting health effectiveness pollinators.

Язык: Английский

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Division of labor in honey bee gut microbiota for plant polysaccharide digestion

Proceedings of the National Academy of Sciences, Год журнала: 2019, Номер 116(51), С. 25909 - 25916

Опубликована: Ноя. 27, 2019

Bees acquire carbohydrates from nectar and lipids; amino acids pollen, which also contains polysaccharides including cellulose, hemicellulose, pectin. These potential energy sources could be degraded fermented through microbial enzymatic activity, resulting in short chain fatty available to hosts. However, the contributions of individual microbiota members polysaccharide digestion have remained unclear. Through analysis bacterial isolate genomes a metagenome honey bee gut microbiota, we identify that Bifidobacterium Gilliamella are principal degraders hemicellulose Both show extensive strain-level diversity gene repertoires linked digestion. Strains bees possess more such genes than strains bumble bees. In Bifidobacterium, encoding carbohydrate-active enzymes colocated within loci devoted utilization, as Bacteroides human gut. Carbohydrate-active enzyme-encoding expressions up-regulated response particular hemicelluloses both vitro vivo. Metabolomic analyses document experimentally colonized by different generate distinctive metabolomic profiles, with enrichment for specific monosaccharides, corresponding predictions genomic data. The other 3 core species clusters (Snodgrassella 2 Lactobacillus clusters) few or no Together, these findings indicate strain composition hosts determines metabolic capabilities potentially affects host nutrition. Furthermore, niche specialization revealed our study may promote overall community stability microbiomes

Язык: Английский

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Honey bees as models for gut microbiota research

Lab Animal, Год журнала: 2018, Номер 47(11), С. 317 - 325

Опубликована: Окт. 11, 2018

Язык: Английский

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Gut microbiota structure differs between honeybees in winter and summer

The ISME Journal, Год журнала: 2019, Номер 14(3), С. 801 - 814

Опубликована: Дек. 13, 2019

Abstract Adult honeybees harbor a specialized gut microbiota of relatively low complexity. While seasonal differences in community composition have been reported, previous studies focused on compositional changes rather than absolute bacterial loads. Moreover, little is known about the winter bees, which live much longer bees during foraging season, and are critical for colony survival. We quantified seven core members bee single over 2 years characterized 14 colonies summer winter. Our data show that total loads substantially differ between foragers, nurses, bees. Long-lived had highest lowest α-diversity, with characteristic shift toward high levels Bartonella Commensalibacter, reduction opportunistic colonizers. Using gnotobiotic experiments, we diet major contributor to observed Overall, our study reveals remarkably different from foragers nurses. Considering importance survival, future work should focus role health disease.

Язык: Английский

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Simple animal models for microbiome research

Nature Reviews Microbiology, Год журнала: 2019, Номер 17(12), С. 764 - 775

Опубликована: Авг. 15, 2019

Язык: Английский

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Functional roles and metabolic niches in the honey bee gut microbiota

Current Opinion in Microbiology, Год журнала: 2018, Номер 43, С. 69 - 76

Опубликована: Янв. 12, 2018

Язык: Английский

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Honeybee gut Lactobacillus modulates host learning and memory behaviors via regulating tryptophan metabolism

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Апрель 19, 2022

Honeybees are highly social insects with a rich behavioral repertoire and versatile model for neurobiological research. Their gut microbiota comprises limited number of host-restricted bacterial phylotypes that important honeybee health. However, it remains unclear how specific members affect behaviors. Here, we find antibiotic exposure disturbs the community influences phenotypes under field conditions. Using laboratory-generated gnotobiotic bees, show normal is required olfactory learning memory abilities. Brain transcriptomic profiling reveals distinct brain gene expression patterns between microbiota-free conventional bees. Subsequent metabolomic analyses both hemolymph samples mainly regulates tryptophan metabolism. Our results indicate host-specific Lactobacillus strains promote behavior by transforming to indole derivatives activate host aryl hydrocarbon receptor. findings highlight contributions neurological processes, thus providing promising understand host-microbe interactions.

Язык: Английский

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Drosophila Antimicrobial Peptides and Lysozymes Regulate Gut Microbiota Composition and Abundance

mBio, Год журнала: 2021, Номер 12(4)

Опубликована: Июль 13, 2021

The gut microbiota affects the physiology and metabolism of animals its alteration can lead to diseases such as dysplasia or metabolic disorders. Several reports have shown that immune system plays an important role in shaping both bacterial community composition abundance Drosophila, deficit, especially during aging, negatively richness diversity. However, there has been little study at effector level demonstrate how pathways regulate microbiota. A key set Drosophila effectors are antimicrobial peptides (AMPs), which confer defense upon systemic infection. AMPs lysozymes, a group digestive enzymes with properties, expressed good candidates for regulation. Here, we take advantage model organism melanogaster investigate lysozymes regulation structure Using flies lacking newly generated lysozyme mutants, colonized gnotobiotic defined commensal bacteria analyzed changes vertical transmission aging contexts through 16S rRNA gene amplicon sequencing. Our shows and, lesser extent, necessary total relative We also decouple direct function from deficiency (IMD) signaling pathway regulates but many other processes, more narrowly defining these microbial dysbiosis observed IMD-deficient aging. IMPORTANCE This advances current knowledge field host-microbe interactions by demonstrating two families effectors, actively abundance. Consequences loss exacerbated their contributes increased shifted old flies. work typically associated resistance pathogenic infections, help shape beneficial community, consistent idea host-symbiont use same "language" pathogenesis.

Язык: Английский

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Standardized multi-omics of Earth’s microbiomes reveals microbial and metabolite diversity

Nature Microbiology, Год журнала: 2022, Номер 7(12), С. 2128 - 2150

Опубликована: Ноя. 28, 2022

Despite advances in sequencing, lack of standardization makes comparisons across studies challenging and hampers insights into the structure function microbial communities multiple habitats on a planetary scale. Here we present multi-omics analysis diverse set 880 community samples collected for Earth Microbiome Project. We include amplicon (16S, 18S, ITS) shotgun metagenomic sequence data, untargeted metabolomics data (liquid chromatography-tandem mass spectrometry gas chromatography spectrometry). used standardized protocols analytical methods to characterize communities, focusing relationships co-occurrences microbially related metabolites taxa environments, thus allowing us explore diversity at extraordinary In addition reference database metabolomic provide framework incorporating additional studies, enabling expansion existing knowledge form an evolving resource. demonstrate utility this by testing hypothesis that every microbe metabolite is everywhere but environment selects. Our results show exhibits turnover nestedness both environment, whereas relative abundances vary co-occur with specific consortia habitat-specific manner. additionally power certain chemistry, particular terpenoids, distinguishing Earth's environments (for example, terrestrial plant surfaces soils, freshwater marine animal stool), as well microbes including Conexibacter woesei (terrestrial soils), Haloquadratum walsbyi (marine deposits) Pantoea dispersa detritus). This Resource provides insight within from Earth, informing chemical ecology, foundation microbiome hosts environment.

Язык: Английский

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Mechanisms underlying gut microbiota–host interactions in insects

Journal of Experimental Biology, Год журнала: 2021, Номер 224(2)

Опубликована: Янв. 15, 2021

ABSTRACT Insects are the most diverse group of animals and colonize almost all environments on our planet. This diversity is reflected in structure function microbial communities inhabiting insect digestive system. As mammals, gut microbiota insects can have important symbiotic functions, complementing host nutrition, facilitating dietary breakdown or providing protection against pathogens. There an increasing number models that experimentally tractable, mechanistic studies microbiota–host interactions. In this Review, we will summarize recent findings advanced understanding molecular mechanisms underlying symbiosis between their microbiota. We open article with a general introduction to then turn towards discussion particular processes governing colonization environment as well beneficial roles mediated by The Review highlights that, although active field research implications for fundamental applied science, still early stage mechanisms. However, expanding capability culture microbiomes manipulate microbe–host interactions promises new insights from symbioses.

Язык: Английский

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