Light-arousal and circadian photoreception circuits intersect at the large PDF cells of the Drosophila brain

Proceedings of the National Academy of Sciences, Год журнала: 2008, Номер 105(50), С. 19587 - 19594

Опубликована: Дек. 6, 2008

The neural circuits that regulate sleep and arousal as well their integration with circadian remain unclear, especially in Drosophila. This issue intersects of photoreception, because light is both an signal diurnal animals entraining for the clock. To identify neurons relevant to light-mediated phase-shifting, we developed genetic techniques link behavior single cell-type resolution within Drosophila central brain. We focused on unknown function 10 PDF-containing large ventral lateral (l-LNvs) brain network show here these cells light-dependent arousal. They also are important phase shifting late-night (dawn), indicating photoresponse a property therefore non-cell-autonomous. data further indicate underlying light-induced photoentrainment intersect at l-LNvs then segregate.

Язык: Английский

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Genetically encoded dendritic marker sheds light on neuronal connectivity inDrosophila

Proceedings of the National Academy of Sciences, Год журнала: 2010, Номер 107(47), С. 20553 - 20558

Опубликована: Ноя. 8, 2010

In recent years, Drosophila melanogaster has emerged as a powerful model for neuronal circuit development, pathology, and function. A major impediment to these studies been the lack of genetically encoded, specific, universal, phenotypically neutral marker somatodendritic compartment. We have developed such show that it is effective specific in all populations tested peripheral central nervous system. The marker, which we name DenMark (Dendritic Marker), hybrid protein mouse ICAM5/Telencephalin red fluorescent mCherry. tool revealing novel aspects neuroanatomy developing dendrites, identifying previously unknown dendritic arbors, elucidating connectivity.

Язык: Английский

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Cryptochrome, Compound Eyes, Hofbauer-Buchner Eyelets, and Ocelli Play Different Roles in the Entrainment and Masking Pathway of the Locomotor Activity Rhythm in the Fruit Fly Drosophila Melanogaster

Journal of Biological Rhythms, Год журнала: 2003, Номер 18(5), С. 377 - 391

Опубликована: Окт. 1, 2003

The fly Drosophila melanogaster possesses five photoreceptors and/or photopigments that appear to be involved in light reception and synchronization of the circadian clock: (1) compound eyes, (2) ocelli, (3) Hofbauer-Buchner eyelets, (4) blue-light photopigment cryptochrome, (5) unknown clock-gene-expressing dorsal neurons. To understand contributions these synchronization, authors monitored flies' activity rhythms under artificial long short days. They found all different photo-pigments contribute significantly entrainment each photoperiod, but eyes are especially important for extreme photoperiods. are, furthermore, necessary adjusting phase rhythm, distinguishing days from constant light, normal masking effects light—namely, promotion by lights-on inhibition darkness. Cryptochrome is period lengthening days, although it more than is, after photoperiod on internal clock. specific roles remaining difficult assess.

Язык: Английский

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The neuroarchitecture of the circadian clock in the brain of Drosophila melanogaster

Microscopy Research and Technique, Год журнала: 2003, Номер 62(2), С. 94 - 102

Опубликована: Сен. 3, 2003

Neuroethologists try to assign behavioral functions certain brain centers, if possible down individual neurons and the expression of specific genes. This approach has been successfully applied for control circadian rhythmic behavior in fruit fly Drosophila melanogaster. Several so-called "clock genes" are expressed lateral dorsal where they generate cell-autonomous molecular oscillations. These clusters connected with each other contribute differentially rhythmicity. report reviews latest work on characterizing pacemaker fly's that activity pupal eclosion, leading questions by which neuronal pathways synchronized external light-dark cycle, how impose periodicity behavior.

Язык: Английский

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Circadian- and Light-Dependent Regulation of Resting Membrane Potential and Spontaneous Action Potential Firing of Drosophila Circadian Pacemaker Neurons

Journal of Neurophysiology, Год журнала: 2007, Номер 99(2), С. 976 - 988

Опубликована: Дек. 13, 2007

The ventral lateral neurons (LNvs) of adult Drosophila brain express oscillating clock proteins and regulate circadian behavior. Whole cell current-clamp recordings large LNvs in freshly dissected whole preparations reveal two spontaneous activity patterns that correlate with underlying membrane potential: tonic burst firing sodium-dependent action potentials. Resting potential are rapidly reversibly regulated by acute changes light intensity. LNv electrophysiological response is attenuated, but not abolished, cry(b) mutant flies hypomorphic for the cell-autonomous light-sensing protein CRYPTOCHROME. electrical regulated, as shown significantly higher resting frequency rate pattern during subjective day relative to night. regulation potential, frequency, closely resemble mammalian neuron characteristics, suggesting a general evolutionary conservation both physiological molecular oscillator mechanisms pacemaker neurons.

Язык: Английский

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Neurobiology of the fruit fly's circadian clock

Genes Brain & Behavior, Год журнала: 2004, Номер 4(2), С. 65 - 76

Опубликована: Сен. 14, 2004

Studying the fruit fly Drosophila melanogaster has revealed mechanisms underlying circadian clock function. Rhythmic behavior could be assessed to function of several genes that generate oscillations in certain brain neurons, which finally modulate a manner. This review outlines how individual pacemaker neurons fly's control rhythm locomotor activity and eclosion.

Язык: Английский

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A comparative view of insect circadian clock systems

Cellular and Molecular Life Sciences, Год журнала: 2009, Номер 67(9), С. 1397 - 1406

Опубликована: Дек. 24, 2009

Язык: Английский

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Circadian Control of Membrane Excitability in Drosophila melanogaster Lateral Ventral Clock Neurons

Journal of Neuroscience, Год журнала: 2008, Номер 28(25), С. 6493 - 6501

Опубликована: Июнь 18, 2008

Drosophila circadian rhythms are controlled by a neural circuit containing ∼150 clock neurons. Although much is known about mechanisms of autonomous cellular oscillation, the connection between oscillation and functional outputs that control physiological behavioral poorly understood. To address this issue, we performed whole-cell patch-clamp recordings on lateral ventral neurons (LN_vs), including large (lLN_vs) small LN_vs (sLN_vs), in situ in adult fly whole-brain explants. We found two distinct sizes action potentials (APs) >50% lLN_vs fire APs spontaneously, determined originate ipsilateral optic lobe contralateral. lLN_v resting membrane potential (RMP), spontaneous AP firing rate, resistance cyclically regulated as function time day 12 h light/dark conditions (LD). RMP becomes more hyperpolarized progresses from dawn to dusk with concomitant decrease rate resistance. From dawn, depolarized, remaining stable. In contrast, defective per⁰ null mutant excitability nearly constant LD. Over 24 darkness (DD), wild-type not regulated, although gradually slightly depolarized. sLN_v most depolarized around lights-on, substantial variability centered lights-off Our results indicate LN_v encodes via clock-dependent mechanism, likely plays critical role regulating behavior.

Язык: Английский

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Photoadaptation in Neurospora by Competitive Interaction of Activating and Inhibitory LOV Domains

Cell, Год журнала: 2010, Номер 142(5), С. 762 - 772

Опубликована: Сен. 1, 2010

Язык: Английский

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Insect photoperiodic calendar and circadian clock: Independence, cooperation, or unity?

Journal of Insect Physiology, Год журнала: 2010, Номер 57(5), С. 538 - 556

Опубликована: Ноя. 3, 2010

Язык: Английский

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