Biochemical and Biophysical Research Communications, Год журнала: 2025, Номер 748, С. 151317 - 151317
Опубликована: Янв. 10, 2025
Язык: Английский
Ageing Research Reviews, Год журнала: 2024, Номер 94, С. 102196 - 102196
Опубликована: Янв. 12, 2024
Язык: Английский
Процитировано
38
Journal of Extracellular Vesicles, Год журнала: 2024, Номер 13(4)
Опубликована: Апрель 1, 2024
Osteoporosis (OP) is a systematic bone disease characterized by low mass and fragile microarchitecture. Conventional treatment for OP has limited efficacy long-term toxicity. Synthetic biology makes bacterial extracellular vesicle (BEVs)-based therapeutic strategies promising alternative the of OP. Here, we constructed recombinant probiotics Escherichia coli Nissle 1917-pET28a-ClyA-BMP-2-CXCR4 (ECN-pClyA-BMP-2-CXCR4), in which BMP-2 CXCR4 were overexpressed fusion with BEVs surface protein ClyA. Subsequently, isolated engineered BEVs-BMP-2-CXCR4 (BEVs-BC) therapy. The BEVs-BC exhibited great targeting vivo. In addition, had good biocompatibility remarkable ability to promote osteogenic differentiation BMSCs. Finally, synthetic biology-based significantly prevented an ovariectomized (OVX) mouse model. conclusion, both bone-targeting bone-forming one-step using biology, provides effective strategy potential industrialization.
Язык: Английский
Процитировано
27
Food & Function, Год журнала: 2024, Номер 15(4), С. 1840 - 1851
Опубликована: Янв. 1, 2024
Osteoporosis has become one of the major diseases that threaten health middle-aged and elderly people, with growth an ageing population, more people are affected by osteoporosis these days. In recent years, intestinal flora been found to affect host immune system, overactive system is closely related bone resorption. Probiotics can effectively improve density strength, reduce loss, osteoporosis, but their mechanism action relationship microbiota still unclear. this study, two strains Bifidobacterium (Bifidobacterium bifidum FL228.1 animalis subsp. Lactis F1-7) alleviate inflammation were screened based on previous experiments. Through construction ovariectomized mouse model, improvement was detected, influence immunity explored. The results show treatment significantly improved mineral (BMD), volume/total volume ratio (BV/TV), trabecular number (Tb·N), suppressed loss. Furthermore, could inhibit expression inflammatory cytokines in gut, gut inflammation, thus suppress excessive osteoclast generation. Its includes factors protect mucosal barrier, including occludin, ZO-1, claudin-2, MUC2, reduction pro-inflammatory M1 macrophages. B. increased abundance beneficial bacteria colon, Lactobacillus Colidextribacter. F1-7 decreased Desulfovibrio Ruminococcus colon. These research findings expand our understanding gut-bone axis provide new guidance for development probiotic-based therapies future.
Язык: Английский
Процитировано
21
Gut Microbes, Год журнала: 2024, Номер 16(1)
Опубликована: Ноя. 24, 2024
A rising corpus of research has shown the beneficial effects probiotic
Язык: Английский
Процитировано
16
Theranostics, Год журнала: 2024, Номер 14(10), С. 3859 - 3899
Опубликована: Янв. 1, 2024
Osteoporosis is a systemic skeletal disease caused by an imbalance between bone resorption and formation. Current treatments primarily involve medication hormone therapy. However, these lack directionality are often ineffective for locally severe osteoporosis, with the potential complex adverse reactions. Consequently, treatment strategies using bioactive materials or external interventions have emerged as most promising approaches. This review proposes twelve microenvironmental targets osteoporosis-related pathological changes, including local accumulation of inflammatory factors reactive oxygen species (ROS), mitochondrial dynamics, insulin resistance, disruption cell autophagy, apoptosis, changes in neural secretions, aging cells, increased tissue vascular destruction, decreased regeneration. Additionally, this examines current research status effective biophysical biochemical stimuli based on summarizes advantages optimal parameters different bioengineering to support preclinical clinical osteoporosis Finally, addresses ongoing challenges future prospects.
Язык: Английский
Процитировано
13
Frontiers in Immunology, Год журнала: 2024, Номер 15
Опубликована: Янв. 30, 2024
Rheumatoid arthritis (RA) and postmenopausal osteoporosis (PMOP) are common bone-immune diseases. The imbalance between helper (Th17) regulatory T cells (Tregs) produced during differentiation of CD4 + plays a key role in bone remodelling disorders RA PMOP. However, the specific mechanism this PMOP has not been clarified. Identifying underlying Th17/Treg represents factor research development new drugs for immune In review, potential roles Th17, Treg, regulating have summarised, mechanisms by which probiotics, traditional Chinese medicine compounds, monomers maintain balance expounded. maintenance could be considered as an therapeutic alternative treatment This study also summarizes advantages disadvantages conventional treatments quality life rehabilitation patients with findings presented her will provide better understanding close relationship immunity chronic diseases ideas future research, prevention,
Язык: Английский
Процитировано
10
Microorganisms, Год журнала: 2024, Номер 12(4), С. 794 - 794
Опубликована: Апрель 14, 2024
To advance probiotic research, a comprehensive understanding of bacterial interactions with human physiology at the molecular and cellular levels is fundamental. Lacticaseibacillus rhamnosus LGG® strain that has long been recognized for its beneficial effects on health. Probiotic effector molecules derived from LGG®, including secreted proteins, surface-anchored polysaccharides, lipoteichoic acids, which interact host physiological processes have identified. In vitro animal studies revealed specific stimulate epithelial cell survival, preserve intestinal barrier integrity, reduce oxidative stress, mitigate excessive mucosal inflammation, enhance IgA secretion, provide long-term protection through epigenetic imprinting. Pili surface promote adhesion to mucosa ensure close contact cells. Extracellular vesicles produced by recapitulate many these their cargo molecules. Collectively, exert significant influence both gut immune system, promotes homeostasis tolerance.
Язык: Английский
Процитировано
10
Microbiological Research, Год журнала: 2024, Номер 286, С. 127821 - 127821
Опубликована: Июнь 25, 2024
Язык: Английский
Процитировано
8
International Immunopharmacology, Год журнала: 2025, Номер 147, С. 114034 - 114034
Опубликована: Янв. 12, 2025
Язык: Английский
Процитировано
1
Frontiers in Nutrition, Год журнала: 2025, Номер 12
Опубликована: Фев. 21, 2025
Respiratory diseases are a leading cause of morbidity in children globally, with significant healthcare costs. The overuse conventional treatments like antibiotics has raised concerns about antibiotic resistance and side effects. Lacticaseibacillus rhamnosus GG (LGG), one the most extensively studied probiotics, gained attention as potential adjunct therapies due to their ability modulate gut microbiota immune responses. This review aims assess effectiveness LGG managing pediatric respiratory diseases, including tract infections (RTI), cystic fibrosis (CF), asthma. Clinical trials suggest can reduce incidence severity RTI, improving CF symptoms, enhancing quality life children. However, evidence for its benefits asthma remains inconclusive. Its mechanisms include modulating responses, barrier function, maintaining microbial homeostasis via gut-lung axis. Existing studies often limited by small sample sizes, heterogeneity intervention protocols, short follow-up periods. Emerging technologies novel formulations, hold promise unraveling complex interactions among LGG, axis, health. These advancements could pave way personalized probiotic therapies, highlighting cost-effective, adjunctive therapy diseases. underscores broader significance integrating into healthcare, while calling future research overcome current limitations, optimize clinical explore innovative therapeutic strategies.
Язык: Английский
Процитировано
1