Journal of Extracellular Vesicles,
Journal Year:
2024,
Volume and Issue:
13(4)
Published: April 1, 2024
Osteoporosis
(OP)
is
a
systematic
bone
disease
characterized
by
low
mass
and
fragile
microarchitecture.
Conventional
treatment
for
OP
has
limited
efficacy
long-term
toxicity.
Synthetic
biology
makes
bacterial
extracellular
vesicle
(BEVs)-based
therapeutic
strategies
promising
alternative
the
of
OP.
Here,
we
constructed
recombinant
probiotics
Escherichia
coli
Nissle
1917-pET28a-ClyA-BMP-2-CXCR4
(ECN-pClyA-BMP-2-CXCR4),
in
which
BMP-2
CXCR4
were
overexpressed
fusion
with
BEVs
surface
protein
ClyA.
Subsequently,
isolated
engineered
BEVs-BMP-2-CXCR4
(BEVs-BC)
therapy.
The
BEVs-BC
exhibited
great
targeting
vivo.
In
addition,
had
good
biocompatibility
remarkable
ability
to
promote
osteogenic
differentiation
BMSCs.
Finally,
synthetic
biology-based
significantly
prevented
an
ovariectomized
(OVX)
mouse
model.
conclusion,
both
bone-targeting
bone-forming
one-step
using
biology,
provides
effective
strategy
potential
industrialization.
Food & Function,
Journal Year:
2024,
Volume and Issue:
15(4), P. 1840 - 1851
Published: Jan. 1, 2024
Osteoporosis
has
become
one
of
the
major
diseases
that
threaten
health
middle-aged
and
elderly
people,
with
growth
an
ageing
population,
more
people
are
affected
by
osteoporosis
these
days.
In
recent
years,
intestinal
flora
been
found
to
affect
host
immune
system,
overactive
system
is
closely
related
bone
resorption.
Probiotics
can
effectively
improve
density
strength,
reduce
loss,
osteoporosis,
but
their
mechanism
action
relationship
microbiota
still
unclear.
this
study,
two
strains
Bifidobacterium
(Bifidobacterium
bifidum
FL228.1
animalis
subsp.
Lactis
F1-7)
alleviate
inflammation
were
screened
based
on
previous
experiments.
Through
construction
ovariectomized
mouse
model,
improvement
was
detected,
influence
immunity
explored.
The
results
show
treatment
significantly
improved
mineral
(BMD),
volume/total
volume
ratio
(BV/TV),
trabecular
number
(Tb·N),
suppressed
loss.
Furthermore,
could
inhibit
expression
inflammatory
cytokines
in
gut,
gut
inflammation,
thus
suppress
excessive
osteoclast
generation.
Its
includes
factors
protect
mucosal
barrier,
including
occludin,
ZO-1,
claudin-2,
MUC2,
reduction
pro-inflammatory
M1
macrophages.
B.
increased
abundance
beneficial
bacteria
colon,
Lactobacillus
Colidextribacter.
F1-7
decreased
Desulfovibrio
Ruminococcus
colon.
These
research
findings
expand
our
understanding
gut-bone
axis
provide
new
guidance
for
development
probiotic-based
therapies
future.
Theranostics,
Journal Year:
2024,
Volume and Issue:
14(10), P. 3859 - 3899
Published: Jan. 1, 2024
Osteoporosis
is
a
systemic
skeletal
disease
caused
by
an
imbalance
between
bone
resorption
and
formation.
Current
treatments
primarily
involve
medication
hormone
therapy.
However,
these
lack
directionality
are
often
ineffective
for
locally
severe
osteoporosis,
with
the
potential
complex
adverse
reactions.
Consequently,
treatment
strategies
using
bioactive
materials
or
external
interventions
have
emerged
as
most
promising
approaches.
This
review
proposes
twelve
microenvironmental
targets
osteoporosis-related
pathological
changes,
including
local
accumulation
of
inflammatory
factors
reactive
oxygen
species
(ROS),
mitochondrial
dynamics,
insulin
resistance,
disruption
cell
autophagy,
apoptosis,
changes
in
neural
secretions,
aging
cells,
increased
tissue
vascular
destruction,
decreased
regeneration.
Additionally,
this
examines
current
research
status
effective
biophysical
biochemical
stimuli
based
on
summarizes
advantages
optimal
parameters
different
bioengineering
to
support
preclinical
clinical
osteoporosis
Finally,
addresses
ongoing
challenges
future
prospects.
Frontiers in Immunology,
Journal Year:
2024,
Volume and Issue:
15
Published: Jan. 30, 2024
Rheumatoid
arthritis
(RA)
and
postmenopausal
osteoporosis
(PMOP)
are
common
bone-immune
diseases.
The
imbalance
between
helper
(Th17)
regulatory
T
cells
(Tregs)
produced
during
differentiation
of
CD4
+
plays
a
key
role
in
bone
remodelling
disorders
RA
PMOP.
However,
the
specific
mechanism
this
PMOP
has
not
been
clarified.
Identifying
underlying
Th17/Treg
represents
factor
research
development
new
drugs
for
immune
In
review,
potential
roles
Th17,
Treg,
regulating
have
summarised,
mechanisms
by
which
probiotics,
traditional
Chinese
medicine
compounds,
monomers
maintain
balance
expounded.
maintenance
could
be
considered
as
an
therapeutic
alternative
treatment
This
study
also
summarizes
advantages
disadvantages
conventional
treatments
quality
life
rehabilitation
patients
with
findings
presented
her
will
provide
better
understanding
close
relationship
immunity
chronic
diseases
ideas
future
research,
prevention,
Microorganisms,
Journal Year:
2024,
Volume and Issue:
12(4), P. 794 - 794
Published: April 14, 2024
To
advance
probiotic
research,
a
comprehensive
understanding
of
bacterial
interactions
with
human
physiology
at
the
molecular
and
cellular
levels
is
fundamental.
Lacticaseibacillus
rhamnosus
LGG®
strain
that
has
long
been
recognized
for
its
beneficial
effects
on
health.
Probiotic
effector
molecules
derived
from
LGG®,
including
secreted
proteins,
surface-anchored
polysaccharides,
lipoteichoic
acids,
which
interact
host
physiological
processes
have
identified.
In
vitro
animal
studies
revealed
specific
stimulate
epithelial
cell
survival,
preserve
intestinal
barrier
integrity,
reduce
oxidative
stress,
mitigate
excessive
mucosal
inflammation,
enhance
IgA
secretion,
provide
long-term
protection
through
epigenetic
imprinting.
Pili
surface
promote
adhesion
to
mucosa
ensure
close
contact
cells.
Extracellular
vesicles
produced
by
recapitulate
many
these
their
cargo
molecules.
Collectively,
exert
significant
influence
both
gut
immune
system,
promotes
homeostasis
tolerance.
Frontiers in Nutrition,
Journal Year:
2025,
Volume and Issue:
12
Published: Feb. 21, 2025
Respiratory
diseases
are
a
leading
cause
of
morbidity
in
children
globally,
with
significant
healthcare
costs.
The
overuse
conventional
treatments
like
antibiotics
has
raised
concerns
about
antibiotic
resistance
and
side
effects.
Lacticaseibacillus
rhamnosus
GG
(LGG),
one
the
most
extensively
studied
probiotics,
gained
attention
as
potential
adjunct
therapies
due
to
their
ability
modulate
gut
microbiota
immune
responses.
This
review
aims
assess
effectiveness
LGG
managing
pediatric
respiratory
diseases,
including
tract
infections
(RTI),
cystic
fibrosis
(CF),
asthma.
Clinical
trials
suggest
can
reduce
incidence
severity
RTI,
improving
CF
symptoms,
enhancing
quality
life
children.
However,
evidence
for
its
benefits
asthma
remains
inconclusive.
Its
mechanisms
include
modulating
responses,
barrier
function,
maintaining
microbial
homeostasis
via
gut-lung
axis.
Existing
studies
often
limited
by
small
sample
sizes,
heterogeneity
intervention
protocols,
short
follow-up
periods.
Emerging
technologies
novel
formulations,
hold
promise
unraveling
complex
interactions
among
LGG,
axis,
health.
These
advancements
could
pave
way
personalized
probiotic
therapies,
highlighting
cost-effective,
adjunctive
therapy
diseases.
underscores
broader
significance
integrating
into
healthcare,
while
calling
future
research
overcome
current
limitations,
optimize
clinical
explore
innovative
therapeutic
strategies.
