Susceptibility of sheep to experimental co-infection with the ancestral lineage of SARS-CoV-2 and its alpha variant

Emerging Microbes & Infections, Год журнала: 2022, Номер 11(1), С. 662 - 675

Опубликована: Фев. 2, 2022

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for a global pandemic that has had significant impacts on human health and economies worldwide. SARS-CoV-2 highly transmissible the cause of disease 2019 in humans. A wide range animal species have also been shown to be susceptible by experimental and/or natural infections. Sheep are commonly farmed domestic ruminant not thoroughly investigated their susceptibility SARS-CoV-2. Therefore, we performed vitro vivo studies which consisted infection ruminant-derived cells challenge sheep investigate Our results showed sheep-derived kidney support replication. Furthermore, demonstrated limited with viral RNA shed nasal oral swabs at 1 3-days post (DPC); was detected tract lymphoid tissues 4 8 DPC. Sero-reactivity observed some principal infected but contact sentinels, indicating transmission co-mingled naïve efficient; however, sentinel animals 21 used inoculum consisting mixture two isolates, representatives ancestral lineage B.1.1.7-like alpha variant concern, study competition virus strains. indicate show low outcompeted strain.

Язык: Английский

---

Reduced airborne transmission of SARS-CoV-2 BA.1 Omicron virus in Syrian hamsters

PLoS Pathogens, Год журнала: 2022, Номер 18(12), С. e1010970 - e1010970

Опубликована: Дек. 2, 2022

Since the start of COVID-19 pandemic, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has caused hundreds millions infections worldwide with more than 6.5 million confirmed deaths.SARS-CoV-2 is a respiratory virus that predominantly infects epithelium upper and lower tract human animal hosts.Transmission viruses between hosts can occur via direct contact, contact infected surfaces, or aerosolized particles [1].Syrian hamsters are highly susceptible to SARS-CoV-2 infection one several have been naturally by this [2-14].Transmission from pet Syrian humans also reported [2].Currently, only rodent model in which airborne transmission easily be tested [2,15].Transmission established detection viral RNA, RNA replication intermediates, viable infectious virus, seroconversion, latter two metrics being most stringent.Since January 2021, National Institutes Health Assessment Variant Evolution (SAVE) initiative evaluated potential variants [16].Several SAVE-affiliated laboratories, using variable exposure times (4 48 hours), inoculum amounts (10 3 10 5 units), experimental designs, observed (strain Wuhan-1 WA1/20202) nearly 100% animals.Similar results were obtained B.1.1.7 (Alpha), B.1.351(Beta), B.1.617(Delta) [15,17,18].Airborne was associated high levels tissues animal, when tested, seroconversion (Fig 1).Certain amino acid mutations (D614G N501Y) found receptor-binding domain spike protein many improved [19-22].Late

Язык: Английский

---

Mouse-Adapted SARS-CoV-2 MA10 Strain Displays Differential Pulmonary Tropism and Accelerated Viral Replication, Neurodissemination, and Pulmonary Host Responses in K18-hACE2 Mice

mSphere, Год журнала: 2023, Номер 8(1)

Опубликована: Фев. 2, 2023

The COVID-19 pandemic, caused by SARS-CoV-2, is still significantly impacting health care systems around the globe. Refined animal models are needed to study SARS-CoV-2 pathogenicity as well efficacy of vaccines and therapeutics.

Язык: Английский

---

Experimental SARS-CoV-2 Infection of Elk and Mule Deer

Emerging infectious diseases, Год журнала: 2024, Номер 30(2)

Опубликована: Янв. 12, 2024

Abstract To assess the susceptibility of elk (Cervus canadensis) and mule deer (Odocoileus hemionus) to SARS-CoV-2, we performed experimental infections in both species. Elk did not shed infectious virus but mounted low-level serologic responses. Mule transmitted pronounced responses thus could play a role SARS-CoV-2 epidemiology.

Язык: Английский

---

ACE2 and TMPRSS2 distribution in the respiratory tract of different animal species and its correlation with SARS-CoV-2 tissue tropism

Microbiology Spectrum, Год журнала: 2024, Номер 12(2)

Опубликована: Янв. 17, 2024

A wide range of animal species show variable susceptibility to SARS-CoV-2; however, host factors associated with varied remain be defined. Here, we examined whether SARS-CoV-2 and virus tropism in different are dependent on the expression distribution receptor angiotensin-converting enzyme 2 (

Язык: Английский

---