Study on the virulence of Metarhizium anisopliae against Spodoptera frugiperda (J. E. Smith, 1797)

Journal of Basic Microbiology, Год журнала: 2024, Номер 64(5)

Опубликована: Фев. 2, 2024

Abstract This study examined the impact of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) conidia on eggs, larvae, pupae, and adults Spodoptera frugiperda . The results showed that exhibited mortality rates were dependent dose. An increased amount (1.5 × 10 9 conidia/mL) was found to be toxic after days treatment, resulting in a 100% rate 98% 76% 85% adults. A using earthworms as bioindicators 3 exposure, M. did not cause any harmful effects earthworms. In contrast, chemical treatment (positive control) resulted at concentration 40 ppm. Histopathological studies earthworm gut tissues treated with fungal show significant differences compared those negative control. monocrotophos damage, notable observed treatment. treatments 70 100 µg/mL solutions Eudrilus eugeniae epidermal mucus no growth. analysis enzymes biochemical level revealed decrease levels acetylcholinesterase, α‐carboxylesterase, β‐carboxylesterase S. larvae exposure conidia. is effective against , highlighting potential this entomopathogenic fungus controlling agricultural insect pest.

Язык: Английский

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Biocontrol effects of chemical molecules derived from Beauveria bassiana against larvae of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Фев. 12, 2024

In this study, we conducted tests on the isolation, identification, characterization, and extraction of chemical molecules from Beauveria bassiana against Tuta absoluta larvae. The enzyme responses T. to crude extract were examined 24 h after treatment, number dead larvae was calculated 48 treatment. Molecular docking studies assess interaction important with acetylcholinesterase enzyme. larvicidal activity chemicals fungi high LC 50 90 values 25.937 33.559 μg/mL, respectively. For a period h, 52.254 60.450 levels acetylcholinesterase, α-carboxylesterase, β-carboxylesterase enzymes lower in treatment group compared control group. GC-MS test revealed that consisted mainly 9,10-octadecadienoic acid, which primary compound. Docking results indicated acid showed strong (AChE). Our findings suggest molecule derived entomopathogenic fungus B. is more toxic We plan conduct its effectiveness semi-field conditions evaluate stability field conditions. believe could be used near future without causing environmental pollution.

Язык: Английский

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Interactions between Entomopathogenic Fungi and Insects and Prospects with Glycans

Journal of Fungi, Год журнала: 2023, Номер 9(5), С. 575 - 575

Опубликована: Май 15, 2023

Concerns regarding the ecological and health risks posed by synthetic insecticides have instigated exploration of alternative methods for controlling insects, such as entomopathogenic fungi (EPF) biocontrol agents. Therefore, this review discusses their use a potential to chemical especially focuses on two major ones, Beauveria bassiana Metarhizium anisopliae, examples. First, exemplifies how B. bassiana- M. anisopliae-based biopesticides are used in world. Then, we discuss mechanism action which EPF interacts with focusing penetration cuticle subsequent death host. The interactions between insect microbiome, well enhancement immune response, also summarized. Finally, presents recent research that N-glycans may play role eliciting an response resulting increased expression immune-related genes smaller peritrophic matrix pores, reducing midgut permeability. Overall, paper provides overview control highlights latest developments relating interaction immunity.

Язык: Английский

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Ecosystem services of entomopathogenic ascomycetes

Journal of Invertebrate Pathology, Год журнала: 2023, Номер 201, С. 108015 - 108015

Опубликована: Ноя. 1, 2023

Entomopathogenic ascomycetes (EA) are an important part of the microbiota in most terrestrial ecosystems, where they can be found regulating natural populations arthropod pests both epigeous and hypogeous habitats while also establishing unique relationships with plants. These fungi offer direct benefits to agriculture human welfare. In present work, we conducted a systematic review comprehensively assess range ecosystem services provided by EA, including indirect pest biocontrol, plant growth promotion, defense against other biotic abiotic stresses, nutrient cycling, production new bioactive compounds agricultural, pharmaceutical medical importance. Moreover, EA compatible microbial macrobial biocontrol agents. This identified need for future research focus on evaluating economic value ecological special emphasis hypocrealean fungi. evaluation is essential not only conservation but better regulation exploitation promoting agricultural sustainability, reducing use chemicals that enter environment, minimizing negative impacts crop protection carbon footprint health.

Язык: Английский

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Endophytic entomopathogenic fungi as biological control agents of insect pests

Pest Management Science, Год журнала: 2024, Номер 80(12), С. 6033 - 6040

Опубликована: Июль 24, 2024

Abstract Entomopathogenic fungi capable of establishing mutualistic endophytic relationships with plants have a tremendous potential as biocontrol agents insect pests. While long played an important and highly effective role in pest suppression, the utility entomopathogenic management is relatively new emerging topic biocontrol. Here we discuss relevance to plant health general, synthesize current knowledge effectiveness against diverse pests, indirect plant‐mediated effects on arthropods, describe benefits that are likely affect herbivores pathogens well. Lastly, consider major challenges incorporating biocontrol, such their non‐target field efficacy, which can be variable influenced by environmental factors. Continued research endophyte–insect–plant–environment interactions critical advancing our these sustainable tactic. © 2024 The Author(s). Pest Management Science published John Wiley & Sons Ltd behalf Society Chemical Industry.

Язык: Английский

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Biology, classification, and entomopathogen-based management and their mode of action on Tuta absoluta (Meyrick) in Asia

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Авг. 7, 2024

, known as the South American tomato leaf miner, significantly impacts plants (

Язык: Английский

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Entomopathogenic fungi promising biocontrol agents for managing lepidopteran pests: Review of current knowledge

Biocatalysis and Agricultural Biotechnology, Год журнала: 2024, Номер 58, С. 103146 - 103146

Опубликована: Апрель 3, 2024

Язык: Английский

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Plant–Entomopathogenic Fungi Interaction: Recent Progress and Future Prospects on Endophytism-Mediated Growth Promotion and Biocontrol

Plants, Год журнала: 2024, Номер 13(10), С. 1420 - 1420

Опубликована: Май 20, 2024

Entomopathogenic fungi, often acknowledged primarily for their insecticidal properties, fulfill diverse roles within ecosystems. These encompass endophytism, antagonism against plant diseases, promotion of the growth plants, and inhabitation rhizosphere, occurring both naturally upon artificial inoculation, as substantiated by a growing body contemporary research. Numerous studies have highlighted beneficial aspects endophytic colonization. This review aims to systematically organize information concerning direct (nutrient acquisition production phytohormones) indirect (resistance induction, antibiotic secondary metabolite production, siderophore mitigation abiotic biotic stresses) implications Furthermore, thorough discussion these mechanisms is provided. Several challenges, including isolation complexities, classification novel strains, impact terrestrial location, vegetation type, anthropogenic reluctance use fungal entomopathogens, been recognized hurdles. However, recent advancements in biotechnology microbial research hold promising solutions many challenges. Ultimately, current constraints delineate potential future avenues leveraging entomopathogens dual control agents.

Язык: Английский

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Enzymatic, cellular breakdown and lysis in treatment of Beauveria Brongniartii on Spodoptera litura (Fabricius, 1775)

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 28, 2025

The current study aimed to isolate Beauveria brongniartii conidia from forest soils, identify the fungus, and evaluate its effectiveness on eggs, larvae, pupae, adults of Spodoptera litura. Insect mortality rates were recorded every 3, 6, 9, 12 days. identification entomopathogenic fungi was carried out using molecular techniques, including PCR, DNA sequencing, markers, detect species-specific 18 S rDNA genetic sequences, all performed under aseptic conditions. results indicated that higher concentrations (2.7 × 109 conidia/mL) exhibited greater virulence, with eggs showing a rate 98.66%, followed by larvae 96%, 90.66%, pupae 77.33% after Probit analysis revealed minimal LC50 LC90 values: (5.5 102; 1.0 106 spores/mL), (8.2 1.2 107 (9.6 104; 7.3 1010 (1.0 103; 2.0 108 spores/mL). total hemocyte counts detailed observational B. induces cellular breakdown cell lysis in S. litura producing enzymes degrade cuticle membranes. Earthworm bioindicator studies showed effects compared controls, while chemical treatments resulted 96% at 100 ppm. Histopathological examinations no significant differences gut tissue between earthworms treated fungal those control group, unlike substantial damage caused treatments. Biochemical alterations enzyme activity, reduced levels phosphatase catalase, as well increased lipid peroxides superoxide dismutase. This highlights controlling litura, demonstrating potential viable biocontrol agent promoting eco-friendly alternatives pesticides, risk non-target species or environment.

Язык: Английский

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Isolation of a highly virulent Metarhizium strain targeting the tea pest, Ectropis obliqua

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Май 15, 2023

Tea is one of the most widely consumed beverages around world. Larvae moth, Ectropis obliqua Prout (Geometridae, Lepidoptera), are destructive insect pests tea in China. E. a polyphagus that increasing concern due to development populations resistant certain chemical insecticides. Microbial biological control agents offer an environmentally friendly and effective means for can be compatible with "green" organic farming practices.To identify novel agents, soil inset cadaver samples were collected from growing regions Fujian province, Isolates analyzed morphologically via molecular characterization identity them at species level. Laboratory greenhouse bioassays used determine effectiveness isolates control.Eleven corresponding ten different Metarhizium identified according morphological analyses and/or cadavers found on plants surrounding sampled eight within Four including M. clavatum, indigoticum, pemphigi, phasmatodeae documented first time China, other as anisopliae, brunneum, lepidiotae, majus, pinghaense, robertsii. Insect eleven revealed significant variation efficacy each isolate infect kill obliqua. pingshaense (MaFZ-13) showed highest virulence reaching host target mortality rate 93% laboratory bioassays. The median lethal concentration (LC50) (LT50) values MaFZ-13 9.6 × 104 conidia/mL 4.8 days, respectively. Greenhouse experiments time-dose-mortality (TDM) models further evaluate confirm fungal pathogenic potential against larvae.Isolation indigenous microbial targeting specific approach collecting resources exploited pest lowered obstacles approval commercialization. Our data show presence four previously unreported Bioassays strains isolated could degrees newly strain representing particularly highly virulent potentially applicable larvae.

Язык: Английский

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