A comprehensive neuroanatomical survey of the Drosophila Lobula Plate Tangential Neurons with predictions for their optic flow sensitivity DOI Open Access
Arthur Zhao, Aljoscha Nern, Sanna Koskela

et al.

Published: Jan. 9, 2024

Flying insects exhibit remarkable navigational abilities controlled by their compact nervous systems. Optic flow , the pattern of changes in visual scene induced locomotion, is a crucial sensory cue for robust self-motion estimation, especially during rapid flight. Neurons that respond to specific, large-field optic patterns have been studied decades, primarily large flies, such as houseflies, blowflies, and hover flies. The best-known optic-flow sensitive neurons are tangential cells dipteran lobula plate, whose visual-motion responses, lesser extent, morphology, explored using single-neuron neurophysiology. Most these studies focused on large, Horizontal Vertical System neurons, yet plate houses much larger set ‘optic-flow’ many which challenging unambiguously identify or reliably target functional studies. Here we report comprehensive reconstruction identification Lobula Plate Tangential an Electron Microscopy (EM) volume whole Drosophila brain. This catalog 58 LPT (per brain hemisphere) contains described here first time provides basis systematic investigation circuitry linking locomotion control. Leveraging computational anatomy methods, estimated motion receptive fields compared tuning consequence body rotations translational movements. We also matched most cases one-for-one basis, stochastically labeled genetic driver lines, mirror-symmetric same EM volume, additional data set. Using cell matches across sets, analyzed integration downstream LPTs find central establish sharper selectivity global than input neurons. Furthermore, found information extracted from processed distinct regions brain, pointing diverse foci generation behaviors.

Language: Английский

Neuronal parts list and wiring diagram for a visual system DOI Creative Commons
Arie Matsliah, Szi-chieh Yu, Krzysztof Kruk

et al.

Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 166 - 180

Published: Oct. 2, 2024

Language: Английский

Citations

29
Connectome-driven neural inventory of a complete visual system DOI Creative Commons
Aljoscha Nern, Frank Loesche, Shin-ya Takemura

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 18, 2024

Vision provides animals with detailed information about their surroundings, conveying diverse features such as color, form, and movement across the visual scene. Computing these parallel spatial requires a large network of neurons, that in distant flies humans, regions comprise half brain's volume. These brain often reveal remarkable structure-function relationships, neurons organized along maps shapes directly relate to roles processing. To unravel stunning diversity complex system, careful mapping neural architecture matched tools for targeted exploration circuitry is essential. Here, we report new connectome right optic lobe from male Drosophila central nervous system FIB-SEM volume comprehensive inventory fly's neurons. We developed computational framework quantify anatomy establishing basis interpreting how vision. By integrating this analysis connectivity information, neurotransmitter identity, expert curation, classified ~53,000 into 727 types, which are systematically described named first time. Finally, share an extensive collection split-GAL4 lines our neuron type catalog. Together, set data unlock possibilities systematic investigations vision Drosophila, foundation deeper understanding sensory

Language: Английский

Citations

28
Connectome-constrained networks predict neural activity across the fly visual system DOI Creative Commons
Janne K. Lappalainen, Fabian Tschopp, Sridhama Prakhya

et al.

Nature, Journal Year: 2024, Volume and Issue: 634(8036), P. 1132 - 1140

Published: Sept. 11, 2024

Language: Английский

Citations

27
Connectome-driven neural inventory of a complete visual system DOI Creative Commons
Aljoscha Nern, Frank Loesche, Shin-ya Takemura

et al.

Nature, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Abstract Vision provides animals with detailed information about their surroundings and conveys diverse features such as colour, form movement across the visual scene. Computing these parallel spatial requires a large network of neurons. Consequently, from flies to humans, regions in brain constitute half its volume. These often have marked structure–function relationships, neurons organized along maps shapes that directly relate roles processing. More than century anatomical studies catalogued detail cell types fly systems 1–3 , behavioural physiological experiments examined capabilities flies. To unravel diversity complex system, careful mapping neural architecture matched tools for targeted exploration this circuitry is essential. Here we present connectome right optic lobe male Drosophila melanogaster acquired using focused ion beam milling scanning electron microscopy. We established comprehensive inventory developed computational framework quantify anatomy. Together, data establish basis interpreting how vision. By integrating analysis connectivity information, neurotransmitter identity expert curation, classified approximately 53,000 into 732 types. are systematically described newly named. Finally, share an extensive collection split-GAL4 lines our neuron-type catalogue. Overall, set unlocks new possibilities systematic investigations vision foundation deeper understanding sensory

Language: Английский

Citations

4
Small-field visual projection neurons detect translational optic flow and support walking control DOI Creative Commons
Matthew Isaacson, Jessica Eliason, Aljoscha Nern

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 22, 2023

SUMMARY Animals rely on visual motion for navigating the world, and research in flies has clarified how neural circuits extract information from moving scenes. However, major pathways connecting these patterns of optic flow to behavior remain poorly understood. Using a high-throughput quantitative assay visually guided behaviors genetic neuronal silencing, we discovered region Drosophila ’s protocerebrum critical following. We used calcium imaging, optogenetics identify single cell type, LPC1, that innervates this region, detects translational flow, plays key role regulating forward walking. Moreover, population LPC1s can estimate travelling direction, such as when gaze direction diverges body heading. By linking specific types their computations behaviors, our findings establish foundation understanding nervous system uses vision guide navigation.

Language: Английский

Citations

27
Social state gates vision using three circuit mechanisms inDrosophila DOI Creative Commons
Catherine E. Schretter, Tom Hindmarsh Sten, Nathan C Klapoetke

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 17, 2024

Abstract Animals are often bombarded with visual information and must prioritize specific features based on their current needs. The neuronal circuits that detect relay have been well-studied. Yet, much less is known about how an animal adjusts its attention as goals or environmental conditions change. During social behaviors, flies need to focus nearby flies. Here, we study the flow of altered when female Drosophila enter aggressive state. From connectome, identified three state-dependent circuit motifs poised selectively amplify response fly-sized objects: convergence excitatory inputs from neurons conveying select internal state; dendritic disinhibition feature detectors; a switch toggles between two detectors. Using cell-type-specific genetic tools, together behavioral neurophysiological analyses, show each these function during aggression. We reveal this same operate in males courtship pursuit, suggesting disparate behaviors may share mechanisms. Our work provides compelling example using connectome infer mechanisms underlie dynamic processing sensory signals.

Language: Английский

Citations

15
Mapping model units to visual neurons reveals population code for social behaviour DOI Creative Commons
Benjamin R. Cowley, Adam J. Calhoun,

Nivedita Rangarajan

et al.

Nature, Journal Year: 2024, Volume and Issue: 629(8014), P. 1100 - 1108

Published: May 22, 2024

Abstract The rich variety of behaviours observed in animals arises through the interplay between sensory processing and motor control. To understand these sensorimotor transformations, it is useful to build models that predict not only neural responses input 1–5 but also how each neuron causally contributes behaviour 6,7 . Here we demonstrate a novel modelling approach identify one-to-one mapping internal units deep network real neurons by predicting behavioural changes arise from systematic perturbations more than dozen neuronal cell types. A key ingredient introduce ‘knockout training’, which involves perturbing during training match experiments. We apply this model transformations Drosophila melanogaster males complex, visually guided social 8–11 visual projection at interface optic lobe central brain form set discrete channels 12 , prior work indicates channel encodes specific feature drive particular 13,14 Our reaches different conclusion: combinations neurons, including those involved non-social behaviours, male interactions with female, forming population code for behaviour. Overall, our framework consolidates effects elicited various into single, unified model, providing map stimulus type behaviour, enabling future incorporation wiring diagrams 15 model.

Language: Английский

Citations

15
Predicting visual function by interpreting a neuronal wiring diagram DOI Creative Commons
H. Sebastian Seung

Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 113 - 123

Published: Oct. 2, 2024

Language: Английский

Citations

11
Sensory quiescence induces a cell-non-autonomous integrated stress response curbed by condensate formation of the ATF4 and XRP1 effectors DOI Creative Commons
Shashank Shekhar, Charles Tracy, Peter V. Lidsky

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 2, 2025

Sensory disabilities have been identified as significant risk factors for dementia but underlying molecular mechanisms are unknown. In different Drosophila models with loss of sensory input, we observe non-autonomous induction the integrated stress response (ISR) deep in brain, indicated by eIF2αS50 phosphorylation-dependent elevated levels ISR effectors ATF4 and XRP1. Unlike during canonical ISR, however, XRP1 transcription enriched cytosolic granules that positive RNA granule markers Caprin, FMR1, p62, reversible upon restoration vision blind flies. Cytosolic restraint dampens expression their downstream targets including genes cell death pathways activated chronic cellular thus constitutes a protective (CSPR). containing both p62 also evident thalamus hippocampus mouse congenital or degenerative blindness. These data indicate conserved link between input curbed responses critical protein quality control brain. Chronic can be damaging hazards they counteract. Here authors show how blindness-induced brain-wide is dampened sequestration granules.

Language: Английский

Citations

2
Heterogeneity of synaptic connectivity in the fly visual system DOI Creative Commons

Jacqueline Cornean,

Sebastian Molina-Obando, Burak Gür

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 21, 2024

Visual systems are homogeneous structures, where repeating columnar units retinotopically cover the visual field. Each of these columns contain many same neuron types that distinguished by anatomic, genetic and - generally functional properties. However, there exceptions to this rule. In 800 Drosophila eye, is an anatomically genetically identifiable cell type with variable properties, Tm9. Since anatomical connectivity shapes neuronal we identified presynaptic inputs several hundred Tm9s across both optic lobes using full adult female fly brain (FAFB) electron microscopic dataset FlyWire connectome. Our work shows Tm9 has three major sparsely distributed inputs. This differs from other Tm neurons, which have only one major, more stereotypic than Genetic synapse labeling showed heterogeneous wiring exists individuals. Together, our data argue system uses heterogeneous, circuit properties achieve robust processing.

Language: Английский

Citations

8