Gut symbiont enhances insecticide resistance in a significant pest, the oriental fruit fly Bactrocera dorsalis (Hendel)

Microbiome, Год журнала: 2017, Номер 5(1)

Опубликована: Янв. 31, 2017

Symbiotic bacteria affect insect physiology and ecology. They may also mediate insecticide resistance within their hosts thereby impact pest vector control practices. Here, we document a novel mechanism of in which gut symbiont the tephritid fruit fly Bactrocera dorsalis enhances to organophosphate trichlorphon.We demonstrated that Citrobacter sp. (CF-BD) plays key role degradation trichlorphon. Based on comparative genomics analysis with other species, phosphatase hydrolase genes were identified CF-BD. These CF-BD had higher expression when trichlorphon was present. inoculated isolated obtained resistance, while antibiotic-treated flies less resistant confirming resistance.Our findings suggest symbiont-mediated can readily develop B. represent more widely relevant than previously recognized.

Язык: Английский

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Detoxifying symbiosis: microbe-mediated detoxification of phytotoxins and pesticides in insects

Natural Product Reports, Год журнала: 2018, Номер 35(5), С. 434 - 454

Опубликована: Янв. 1, 2018

Symbiotic microorganisms degrade natural and artificial toxic compounds, confer toxin resistance on insect hosts.

Язык: Английский

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The microbiome of pest insects: it is not just bacteria

Entomologia Experimentalis et Applicata, Год журнала: 2019, Номер 167(3), С. 156 - 170

Опубликована: Фев. 27, 2019

Abstract Insects are associated with multiple microbes that have been reported to influence various aspects of their biology. Most studies in insects, including pest species, focus on the bacterial communities microbiome even though consists members many more kingdoms, which can also large life history insects. In this review, we present some key examples how different microbiome, such as bacteria, fungi, viruses, archaea, and protozoa, affect fitness behavior Moreover, argue interactions within among microbial groups abundant great importance, necessitating use a community approach study microbial–host interactions. We propose restricted bacteria very likely hampers our understanding functioning impact biology close review by highlighting few open questions provide an in‐depth other components addition might host performance, thus contributing insect ecology.

Язык: Английский

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Symbiotic bacteria enable olive fly larvae to overcome host defences

Royal Society Open Science, Год журнала: 2015, Номер 2(7), С. 150170 - 150170

Опубликована: Июль 1, 2015

Ripe fruit offer readily available nutrients for many animals, including fly larvae (Diptera: Tephritidae) and their associated rot-inducing bacteria. Yet, during most of ontogeny, remain chemically defended effectively suppress herbivores pathogens by high levels secondary metabolites. Olive flies ( Bactrocera oleae ) are uniquely able to develop in unripe olives. Unlike other frugivorous tephritids, the maintain bacteria confined within midgut caeca. We examined interaction between larvae, bacteria, chemical defence, hypothesizing that bacterial contribution larval development is contingent on phenology defensive chemistry. demonstrate require natural complement Candidatus Erwinia dacicola: Enterobacteriaceae) order Conversely, when feeding ripe fruit, proceeds independently these Our experiments suggest counteract inhibitory effect oleuropein—the principal phenolic glycoside In light results, we unique symbiosis olive flies, compared with understood considering relationship fly, When applied an evolutionary context, this approach may also point out forces which shaped symbioses across Tephritidae.

Язык: Английский

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Detoxifying symbionts in agriculturally important pest insects

Microbial Biotechnology, Год журнала: 2016, Номер 10(3), С. 531 - 540

Опубликована: Дек. 12, 2016

Pest insects lead to excessive agricultural and therefore economical losses on crops worldwide. These have withstand toxic molecules that are inherent plant defences, as well those produced introduced by humans in the form of insecticides. In recent years, research insect-microbe symbioses has recognized microbial symbionts may play a role protecting against these toxins, leading defensive symbiosis between pest insect different types microorganisms we term detoxifying symbioses. this minireview, will highlight well-characterized emerging model systems assess how influence host's success.

Язык: Английский

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Current knowledge of interactions between Drosophila suzukii and microbes, and their potential utility for pest management

Journal of Pest Science, Год журнала: 2016, Номер 89(3), С. 621 - 630

Опубликована: Апрель 25, 2016

Язык: Английский

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Gut microbiota promotes host resistance to low-temperature stress by stimulating its arginine and proline metabolism pathway in adult Bactrocera dorsalis

PLoS Pathogens, Год журнала: 2020, Номер 16(4), С. e1008441 - e1008441

Опубликована: Апрель 15, 2020

Gut symbiotic bacteria have a substantial impact on host physiology and ecology. However, the contribution of gut microbes to fitness during long-term low-temperature stress is still unclear. This study examined role microbiota in resistance at molecular biochemical levels oriental fruit fly Bactrocera dorsalis. The results showed that after flies were removed via antibiotic treatment, median survival time was significantly decreased approximately 68% conventional following exposure temperature 10°C. Furthermore, we found Klebsiella michiganensis BD177 key bacterium, whose recolonization treated (ABX) extended 160% ABX control, restored their lifespan level flies. Notably, relative proline arginine metabolites downregulated by 34- 10-fold, respectively, compared with those hemolymph 10°C whereas K. upregulated 13- 10- fold, qPCR analysis also confirmed michiganensis-recolonized stimulated expression transcripts from metabolism pathway controls, RNAi mediated silencing two genes Pro-C ASS reduced flies, postexposure stress. We show microinjection L-arginine L-proline into increased Transmission electron microscopy (TEM) further revealed caused severe destruction cristae structures thus resulted abnormal circular shapes mitochondria gut, while live helped maintain mitochondrial functionality normal status, which important for induction. Our suggest plays vital promoting B. dorsalis stimulating its pathway.

Язык: Английский

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The Gut Microbiota in Camellia Weevils Are Influenced by Plant Secondary Metabolites and Contribute to Saponin Degradation

mSystems, Год журнала: 2020, Номер 5(2)

Опубликована: Март 16, 2020

The camellia weevil (CW [Curculio chinensis]) is a notorious host-specific predator of the seeds Camellia species in China, causing seed losses up to 60%. capable overcoming host tree chemical defenses, while mechanisms how these beetles contend with toxic compounds are still unknown. Here, we examined interaction between gut microbes CW and chemistry found that beetle-associated bacterial symbionts mediate tea saponin degradation. We demonstrate microbial community profile was significantly plant associated, associated feeding on oleifera enriched genes involved degradation compared those sinensis reticulata seeds. Twenty-seven bacteria from genera Enterobacter, Serratia, Acinetobacter, Micrococcus subsisted as sole source carbon nitrogen, Acinetobacter identified being saponin. Our results provide first metagenome communities specialist insect pest trees, consistent potential contribution detoxification tree-defensive chemicals.IMPORTANCE microbiome may play an important role insect-plant interactions mediated by secondary metabolites, but functions feeders poorly characterized. In present study, saponin-rich saponin-low seeds, reveal correlation diversity allelochemicals. also used cultured establish their saponin-degradative capacity outside insect. new experimental context better understand influenced metabolites resistance involving have evolved deal defenses plants.

Язык: Английский

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Gut microbiota degrades toxic isothiocyanates in a flea beetle pest

Molecular Ecology, Год журнала: 2020, Номер 29(23), С. 4692 - 4705

Опубликована: Окт. 2, 2020

Microbial symbionts of herbivorous insects have been suggested to aid in the detoxification plant defense compounds; however, quantitative studies on microbial contribution toxin degradation remain scarce. Here, we demonstrate microbiome-mediated plant-derived toxic isothiocyanates cabbage stem flea beetle Psylliodes chrysocephala, a major pest oilseed rape. Suppression microbiota antibiotic-fed beetles resulted up 11.3-fold higher levels unmetabolized compared control but did not affect other known pathways P. chrysocephala. We characterized microbiome laboratory-reared and field-collected using 16S rRNA amplicon sequencing isolated bacteria belonging three core genera Pantoea, Acinetobacter Pseudomonas. Only Pantoea isolates rapidly degraded vitro, restored isothiocyanate vivo when reintroduced beetles. was consistently present across life stages field lab populations. In addition, detected undamaged tissues host Brassica rapa, indicating that chrysocephala could possibly acquire an detoxifying bacterium through their diet. Our results both insect endogenous mechanisms can contribute compounds together they better account for fate ingested metabolites.

Язык: Английский

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Utilizing Olive Fly Ecology Towards Sustainable Pest Management

Biology, Год журнала: 2025, Номер 14(2), С. 125 - 125

Опубликована: Янв. 25, 2025

The olive fly (Bactrocera oleae, OLF) is a major pest of global significance that occurs in places where cultivation thrives. This paper highlights the economic and environmental damage caused by OLF infestations, including reduced oil yield quality, disrupted supply chains, ecosystem imbalances due to heavy insecticide use. Understanding ecology crucial for developing effective control strategies. review explores fly’s life cycle, its relationship with trees, how factors like temperature humidity influence population dynamics. Additionally, studying role natural enemies agricultural practices can pave way sustainable methods minimize harm. Climate change, intensive cultivation, development resistance insecticides necessitate shift towards practices. includes exploring alternative biological attract-and-kill Furthermore, deeper understanding ecology, response ability find refuge diverse landscapes, critical predicting outbreaks implementing protection By employing holistic approach integrates ecological knowledge methods, we ensure continued viability protect environment, produce high-quality oil.

Язык: Английский

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