Virulence Factors in Hypervirulent Klebsiella pneumoniae

Frontiers in Microbiology, Год журнала: 2021, Номер 12

Опубликована: Апрель 8, 2021

Hypervirulent Klebsiella pneumoniae (hvKP) has spread globally since first described in the Asian Pacific Rim. It is an invasive variant that differs from classical K. (cKP), with hypermucoviscosity and hypervirulence, causing community-acquired infections, including pyogenic liver abscess, pneumonia, meningitis, endophthalmitis. utilizes a battery of virulence factors for survival pathogenesis, such as capsule, siderophores, lipopolysaccharide, fimbriae, outer membrane proteins, type 6 secretion system, which former two are dominant. This review summarizes these hvKP-associated order to understand its molecular pathogenesis shed light on new strategies improve prevention, diagnosis, treatment hvKP-causing infection.

Язык: Английский

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Tracking key virulence loci encoding aerobactin and salmochelin siderophore synthesis in Klebsiella pneumoniae

Genome Medicine, Год журнала: 2018, Номер 10(1)

Опубликована: Окт. 24, 2018

Klebsiella pneumoniae is a recognised agent of multidrug-resistant (MDR) healthcare-associated infections; however, individual strains vary in their virulence potential due to the presence mobile accessory genes. In particular, gene clusters encoding biosynthesis siderophores aerobactin (iuc) and salmochelin (iro) are associated with invasive disease common amongst hypervirulent K. clones that cause severe community-associated infections such as liver abscess pneumonia. Concerningly, iuc has also been reported MDR hospital setting, where it was increased mortality, highlighting need understand, detect track mobility these loci population.

Язык: Английский

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Klebsiella variicola : an emerging pathogen in humans

Emerging Microbes & Infections, Год журнала: 2019, Номер 8(1), С. 973 - 988

Опубликована: Янв. 1, 2019

The Klebsiella pneumoniae complex comprises seven K. pneumoniae-related species, including variicola. variicola is a versatile bacterium capable of colonizing different hosts such as plants, humans, insects and animals. Currently, gaining recognition cause several human infections; nevertheless, its virulence profile not fully characterized. clinical significance infection hidden by imprecise detection methods that underestimate real prevalence; however, have been developed to correctly identify this species. Recent studies carbapenemase-producing colistin-resistant strains demonstrate potential reservoir multidrug-resistant genes. This finding presents an imminent scenario for spreading antimicrobial resistant genes among close relatives and, more concerningly, in environmental settings. Since was identified novel bacterial research groups contributed findings elucidating pathogen; important details about epidemiology, pathogenesis ecology are still missing. review highlights the most significant aspects variicola, discussing phenotypes, mechanisms resistance, traits, well types infections associated with pathogen.

Язык: Английский

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The Role of Integrative and Conjugative Elements in Antibiotic Resistance Evolution

Trends in Microbiology, Год журнала: 2020, Номер 29(1), С. 8 - 18

Опубликована: Июнь 11, 2020

Язык: Английский

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Genomic dissection of Klebsiella pneumoniae infections in hospital patients reveals insights into an opportunistic pathogen

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Май 31, 2022

Abstract Klebsiella pneumoniae is a major cause of opportunistic healthcare-associated infections, which are increasingly complicated by the presence extended-spectrum beta-lactamases (ESBLs) and carbapenem resistance. We conducted year-long prospective surveillance study K. clinical isolates in hospital patients. Whole-genome sequence (WGS) data reveals diverse pathogen population, including other species within complex (18%). Several infections were caused variicola/K. hybrids, one shows evidence nosocomial transmission. A wide range antimicrobial resistance (AMR) phenotypes observed, genetic mechanisms identified (mainly plasmid-borne genes). ESBLs correlated with acquired AMR genes (median n = 10). Bacterial genomic features associated onset (OR 2.34, p 0.015) rhamnose-positive capsules 3.12, < 0.001). Virulence plasmid-encoded (aerobactin, hypermucoidy) observed at low-prevalence (<3%), mostly community-onset cases. WGS-confirmed transmission implicated just 10% cases, but strongly 21, 1 × 10 −11 ). estimate 28% risk onward for ESBL-positive strains vs 1.7% ESBL-negative strains. These indicate that hospitalised patients due largely to strains, an additional burden from nosocomially-transmitted community-acquired hypervirulent

Язык: Английский

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A large-scale genomic snapshot of Klebsiella spp. isolates in Northern Italy reveals limited transmission between clinical and non-clinical settings

Nature Microbiology, Год журнала: 2022, Номер 7(12), С. 2054 - 2067

Опубликована: Ноя. 21, 2022

Abstract The Klebsiella group, found in humans, livestock, plants, soil, water and wild animals, is genetically ecologically diverse. Many species are opportunistic pathogens can harbour diverse classes of antimicrobial resistance genes. Healthcare-associated pneumoniae clones that non-susceptible to carbapenems spread rapidly, representing a high public health burden. Here we report an analysis 3,482 genome sequences 15 sampled over 17-month period from wide range clinical, community, animal environmental settings around the Italian city Pavia. Northern Italy hotspot for hospital-acquired carbapenem thus pertinent setting examine overlap between isolates clinical non-clinical settings. We no genotypic or phenotypic evidence non-susceptibility outside environment. Although noted occasional transmission settings, our data point limited role reservoirs human acquisition spp. also provide detailed genus-wide view genomic diversity population structure, including identification new groups.

Язык: Английский

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Intestinal bacteria and colorectal cancer: etiology and treatment

Gut Microbes, Год журнала: 2023, Номер 15(1)

Опубликована: Март 16, 2023

The etiology of colorectal cancer (CRC) is influenced by bacterial communities that colonize the gastrointestinal tract. These microorganisms derive essential nutrients from indigestible dietary or host-derived compounds and activate molecular signaling pathways necessary for normal tissue immune function. Associative mechanistic studies have identified species whose presence may increase CRC risk, including notable examples such as Fusobacterium nucleatum, Enterotoxigenic Bacteroides fragilis, pks+ E. coli. In recent years this work has expanded in scope to include aspects host mutational status, intra-tumoral microbial heterogeneity, transient infection, cumulative influence multiple carcinogenic bacteria after sequential co-colonization. review, we will provide an updated overview how host-bacteria interactions development, knowledge be utilized diagnose prevent CRC, gut microbiome influences treatment efficacy.

Язык: Английский

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“Superbugs” with hypervirulence and carbapenem resistance in Klebsiella pneumoniae: the rise of such emerging nosocomial pathogens in China

Science Bulletin, Год журнала: 2023, Номер 68(21), С. 2658 - 2670

Опубликована: Сен. 30, 2023

Although hypervirulent Klebsiella pneumoniae (hvKP) can produce community-acquired infections that are fatal in young and adult hosts, such as pyogenic liver abscess, endophthalmitis, meningitis, it has historically been susceptible to antibiotics. Carbapenem-resistant K. (CRKP) is usually associated with urinary tract acquired hospitals, pneumonia, septicemias, soft tissue infections. Outbreaks quick spread of CRKP hospitals have become a major challenge public health due the lack effective antibacterial treatments. In early stages development, HvKP first appear distinct routes. However, lines dividing two pathotypes vanishing currently, advent carbapenem-resistant (CR-hvKP) devastating simultaneously multidrug-resistant, hypervirulent, highly transmissible. Most CR-hvKP cases reported Asian clinical settings, particularly China. Typically, develops when hvKP or acquires plasmids carry either carbapenem-resistance gene virulence gene. Alternatively, classic (cKP) may acquire hybrid plasmid carrying both genes. this review, we provide an overview key antimicrobial resistance mechanisms, factors, presentations, outcomes infection. Additionally, discuss possible evolutionary processes prevalence Given wide occurrence CR-hvKP, continued surveillance control measures organisms should be assigned higher priority.

Язык: Английский

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From Theory to Practice: Translating Whole-Genome Sequencing (WGS) into the Clinic

Trends in Microbiology, Год журнала: 2018, Номер 26(12), С. 1035 - 1048

Опубликована: Сен. 4, 2018

Язык: Английский

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Emergence and rapid global dissemination of CTX-M-15-associatedKlebsiella pneumoniaestrain ST307

Journal of Antimicrobial Chemotherapy, Год журнала: 2018, Номер 74(3), С. 577 - 581

Опубликована: Ноя. 13, 2018

Recent reports indicate the emergence of a new carbapenemase-producing Klebsiella pneumoniae clone, ST307. We sought to better understand global epidemiology and evolution this clone evaluate its association with antimicrobial resistance (AMR) genes.We collated information from literature public databases performed comparative analysis 95 ST307 genomes (including 37 that were newly sequenced).We show emerged in mid-1990s (nearly 20 years prior first report), is already globally distributed intimately associated conserved plasmid harbouring blaCTX-M-15 ESBL gene several other AMR determinants.Our findings support need for enhanced surveillance widespread which carbapenem has occasionally emerged.

Язык: Английский

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