Klebsiella species: Taxonomy, hypervirulence and multidrug resistance

EBioMedicine, Год журнала: 2022, Номер 79, С. 103998 - 103998

Опубликована: Апрель 8, 2022

Members of the genus Klebsiella have rapidly evolved within past decade, generating organisms that simultaneously exhibit both multidrug resistance and hypervirulence (MDR-hv) phenotypes; such are associated with severe hospital- community-acquired infections. Carbapenem-resistant infections unknown optimal treatment regime were particular concern among MDR-hv strains. Recent studies revealed molecular features mobile elements they harbour, allowing identification genetic loci responsible for transmission, stable inheritance, expression or virulence-encoding confer new phenotypic characteristics spp. Here, we provide a comprehensive review on taxonomic position, species composition different phylotypes spp., describing diversity worldwide distribution clones, mutation horizontal gene transfer events drive evolution potential impact human health.

Язык: Английский

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“Superbugs” with hypervirulence and carbapenem resistance in Klebsiella pneumoniae: the rise of such emerging nosocomial pathogens in China

Science Bulletin, Год журнала: 2023, Номер 68(21), С. 2658 - 2670

Опубликована: Сен. 30, 2023

Although hypervirulent Klebsiella pneumoniae (hvKP) can produce community-acquired infections that are fatal in young and adult hosts, such as pyogenic liver abscess, endophthalmitis, meningitis, it has historically been susceptible to antibiotics. Carbapenem-resistant K. (CRKP) is usually associated with urinary tract acquired hospitals, pneumonia, septicemias, soft tissue infections. Outbreaks quick spread of CRKP hospitals have become a major challenge public health due the lack effective antibacterial treatments. In early stages development, HvKP first appear distinct routes. However, lines dividing two pathotypes vanishing currently, advent carbapenem-resistant (CR-hvKP) devastating simultaneously multidrug-resistant, hypervirulent, highly transmissible. Most CR-hvKP cases reported Asian clinical settings, particularly China. Typically, develops when hvKP or acquires plasmids carry either carbapenem-resistance gene virulence gene. Alternatively, classic (cKP) may acquire hybrid plasmid carrying both genes. this review, we provide an overview key antimicrobial resistance mechanisms, factors, presentations, outcomes infection. Additionally, discuss possible evolutionary processes prevalence Given wide occurrence CR-hvKP, continued surveillance control measures organisms should be assigned higher priority.

Язык: Английский

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General Overview of Klebsiella pneumonia: Epidemiology and the Role of Siderophores in Its Pathogenicity

Biology, Год журнала: 2024, Номер 13(2), С. 78 - 78

Опубликована: Янв. 27, 2024

The opportunistic pathogen Klebsiella pneumoniae (K. pneumoniae) can colonize mucosal surfaces and spread from mucosae to other tissues, causing fatal infections. Medical equipment the healthcare setting become colonized by species, which are widely distributed in nature be found water, soil, animals. Moreover, a substantial number of community-acquired illnesses also caused this organism worldwide. These infections characterized high rate morbidity mortality as well capacity metastatically. Hypervirulent strains thought connected these Four components critical bacterium’s pathogenicity—the capsule, lipopolysaccharide, fimbriae, siderophores. Siderophores secondary metabolites that allow iron sequester surrounding medium transport it intracellular compartment bacteria. A variables may lead K. colonization specific area. Risk factors for infection include local practices, antibiotic use misuse, control procedures, nutrition, gender, age.

Язык: Английский

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Vaccine value profile for Klebsiella pneumoniae

Vaccine, Год журнала: 2024, Номер 42(19), С. S125 - S141

Опубликована: Март 19, 2024

Klebsiella pneumoniae causes community- and healthcare-associated infections in children adults. Globally 2019, an estimated 1.27 million (95% Uncertainty Interval [UI]: 0.91-1.71) 4.95 UI: 3.62-6.57) deaths were attributed to associated with bacterial antimicrobial resistance (AMR), respectively. K. was the second leading pathogen AMR resistant bacteria. Furthermore, rise of both hospital-acquired is a concern for neonates infants who are at high risk invasive disease. There limited antibiotic pipeline new antibiotics treat multidrug infections, vaccines targeted against considered be priority by World Health Organization. Vaccination pregnant women could reduce K.pneumoniae disease their young offspring. In addition, vulnerable children, adolescents adult populations underlying diseases such as immunosuppression from hematologic malignancy, chemotherapy, patients undergoing abdominal and/or urinary surgical procedures, or prolonged intensive care management also potential target groups vaccine. A 'Vaccine Value Profile' (VVP) K.pneumoniae, which contemplates vaccination protect babies birth through least three months age other high-risk populations, provides high-level, holistic assessment available information inform public health, economic societal value preventatives therapeutics. This VVP developed working group subject matter experts academia, non-profit organizations, public-private partnerships, multi-lateral collaboration stakeholders WHO. All contributors have extensive expertise on various elements collectively aimed identify current research knowledge gaps. The using only existing publicly information.

Язык: Английский

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Hypervirulent and carbapenem-resistant Klebsiella pneumoniae: A global public health threat

Microbiological Research, Год журнала: 2024, Номер 288, С. 127839 - 127839

Опубликована: Авг. 11, 2024

Язык: Английский

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Klebsiella pneumoniae K2 capsular polysaccharide degradation by a bacteriophage depolymerase does not require trimer formation

mBio, Год журнала: 2024, Номер 15(3)

Опубликована: Фев. 13, 2024

ABSTRACT K2-capsular Klebsiella pneumoniae is a hypervirulent pathogen that causes fatal infections. Here, we describe phage tailspike protein, named K2-2, specifically depolymerizes the K2 capsular polysaccharide (CPS) of K. into tetrasaccharide repeating units. Nearly half products contained O -acetylation, which was thought crucial to immunogenicity CPS. The product-bound structures this trimeric enzyme revealed intersubunit carbohydrate-binding grooves, each accommodating three units catalytic residues and key interactions responsible for CPS recognition were identified verified by site-directed mutagenesis. Further biophysical functional characterization, along with structure tetrameric form demonstrated formation center does not require trimerization, could be nearly completely disrupted single-residue mutation in C-terminal domain. Our findings regarding assembly catalysis K2-2 provide cues development glycoconjugate vaccines against infection. IMPORTANCE Generating fragments polysaccharides from pathogenic bacteria antigenic determinants vaccine continues pose challenges. significance region protein (TSP) relation its folding trimer remains largely unexplored. depolymerase described here demonstrates ability depolymerize while retaining vital -acetylation modification immunogenicity. By carefully characterizing enzyme, elucidating three-dimensional structures, conducting mutagenesis, assessing antimicrobial efficacy mutant enzymes , offer valuable insights mechanism recognizes findings, particularly discovery required depolymerizing activity, challenge current understanding trimer-dependent TSP activity highlight an center.

Язык: Английский

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Genomic Analysis of Virulent, Multidrug Resistant Klebsiella pneumoniae and Klebsiella oxytoca from Bloodstream Infections, South Africa

Microbial Pathogenesis, Год журнала: 2025, Номер unknown, С. 107272 - 107272

Опубликована: Янв. 1, 2025

The study investigated the resistome, virulome and mobilome of multidrug resistant (MDR) Klebsiella pneumoniae oxytoca clinical isolates. A total 46 suspected species (spp.) were collected from blood cultures within uMgungundlovu District in KwaZulu-Natal Province. Antibiotic susceptibility was determined against a panel 19 antibiotics using disk diffusion test. subset 14 MDR K. (n=10) (n=4) isolates selected based on their antibiograms subjected to whole genome sequencing (WGS). sequence types (STs), virulome, mobilome, capsule loci (KLs) analysed relevant WGS bioinformatics tools. Of 10 (ST) identified, most common ST25 (n=3), ST101 4 belonged ST450 (n=3). two high-risk clones ST15, ST17 identified. O K with predominance KL2, KL17, KL29, O1/O2v2, O1/O2v1, OL104 respectively. majority displayed resistance predominantly carrying β-lactamase genes, including bla convergence hypervirulence genes strains is potential concern. Carbapenemase, ESBL screening genomic surveillance are urgently required hospital environments.

Язык: Английский

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Hypervirulent Klebsiella pneumoniae have better clinical outcomes than classical Klebsiella pneumoniae for lower respiratory tract infection patients

BMC Microbiology, Год журнала: 2025, Номер 25(1)

Опубликована: Янв. 22, 2025

Язык: Английский

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Emerging Challenges in Klebsiella pneumoniae: Antimicrobial Resistance and Novel Approach

Microbial Pathogenesis, Год журнала: 2025, Номер unknown, С. 107399 - 107399

Опубликована: Фев. 1, 2025

Язык: Английский

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Hypervirulent Klebsiella pneumoniae Endogenous Endophthalmitis—A Global Emerging Disease

Life, Год журнала: 2021, Номер 11(7), С. 676 - 676

Опубликована: Июль 10, 2021

The review aims to document the new emerging hypervirulent Klebsiella pneumoniae (Kp) endogenous endophthalmitis (EKE) in terms of incidence, microbiological characterization pathogenic agent, associated risk factors, management, and outcomes. Hypervirulent (hv) strains KP (hvKp) induce invasive liver abscesses (LA) with specific clinical features. Up 80–90% cases have hepatic abscess as a primary focus infection, followed by renal or lung hvKp infections. However, incidence EKE patients KPLA varied between 3.4% (19) 12.6% (13), total 95 1455 (6.5%). Severe visual loss was encountered 75% cases, 25% bilateral involvement. Intravitreal antibiotics are mainstay therapeutic approach. Pars plana vitrectomy is subject controversy. HvKp present mostly natural “wild-type” antibiotic resistance profile suggestive for community-acquired infections, being highly susceptive third fourth generation cephalosporins carbapenems. Antimicrobial recently documented via plasmid transfer may result extremely difficult treat cases. Global dissemination these major epidemiologic shift that should be considered diagnostic management endophthalmitis. Ophthalmologic screening other infections multidisciplinary approach important early diagnosis preservation function.

Язык: Английский

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