Nucleic Acids Research,
Год журнала:
2018,
Номер
46(15), С. e90 - e90
Опубликована: Май 8, 2018
In
contrast
to
genome
editing,
which
introduces
genetic
changes
at
the
DNA
level,
disrupting
or
editing
gene
transcripts
provides
a
distinct
approach
perturbing
system,
offering
benefits
complementary
classic
approaches.
To
develop
new
toolset
for
manipulating
RNA,
we
first
implemented
member
of
type
VI
CRISPR
systems,
Cas13a
from
Leptotrichia
shahii
(LshCas13a),
in
Schizosaccharomyces
pombe,
an
important
model
organism
employed
by
biologists
study
key
cellular
mechanisms
conserved
yeast
humans.
This
was
shown
knock
down
targeted
endogenous
with
different
efficiencies.
Second,
engineered
RNA
system
tethering
inactive
form
LshCas13a
(dCas13)
catalytic
domain
human
adenosine
deaminase
acting
on
2
(hADAR2d),
be
programmable
crRNA
target
messenger
RNAs
and
precisely
edit
specific
nucleotide
residues.
We
optimized
parameters
using
dual-fluorescence
reporter
demonstrated
utility
randomly
selected
transcripts.
further
used
it
restore
transposition
retrotransposon
Tf1
mutants
fission
yeast,
providing
potential
novel
retrovirus
manipulation
interference.
Cold Spring Harbor Perspectives in Biology,
Год журнала:
2014,
Номер
6(12), С. a019315 - a019315
Опубликована: Дек. 1, 2014
Craig
S.
Pikaard1
and
Ortrun
Mittelsten
Scheid2
1Department
of
Biology,
Department
Molecular
Cellular
Biochemistry,
Howard
Hughes
Medical
Institute,
Indiana
University,
Bloomington,
47405
2Gregor
Mendel-Institute
Plant
Austrian
Academy
Sciences,
1030
Vienna,
Austria
Correspondence:
ortrun.mittelsten_scheid{at}gmi.oeaw.ac.at
Cold Spring Harbor Perspectives in Biology,
Год журнала:
2015,
Номер
7(1), С. a019364 - a019364
Опубликована: Янв. 1, 2015
Steven
Henikoff1
and
M.
Mitchell
Smith2
1Howard
Hughes
Medical
Institute,
Fred
Hutchinson
Cancer
Research
Center,
Seattle,
Washington
98109-1024
2Department
of
Microbiology,
University
Virginia,
Charlottesville,
Virginia
22908
Correspondence:
steveh{at}fhcrc.org
Genetics,
Год журнала:
2015,
Номер
201(2), С. 403 - 423
Опубликована: Окт. 1, 2015
Abstract
The
fission
yeast
Schizosaccharomyces
pombe
is
an
important
model
organism
for
the
study
of
eukaryotic
molecular
and
cellular
biology.
Studies
S.
pombe,
together
with
studies
its
distant
cousin,
Saccharomyces
cerevisiae,
have
led
to
discovery
genes
involved
in
fundamental
mechanisms
transcription,
translation,
DNA
replication,
cell
cycle
control,
signal
transduction,
name
but
a
few
processes.
However,
since
divergence
two
species
approximately
350
million
years
ago,
appears
evolved
less
rapidly
than
cerevisiae
so
that
it
retains
more
characteristics
common
ancient
ancestor,
causing
share
features
metazoan
cells.
This
Primer
introduces
by
describing
itself,
providing
brief
description
origins
research,
illustrating
some
genetic
bioinformatics
tools
used
protein
function
yeast.
In
addition,
section
on
key
differences
between
included
readers
familiarity
budding
research
who
may
interest
developing
projects
using
pombe.
PLoS Biology,
Год журнала:
2019,
Номер
17(12), С. e3000559 - e3000559
Опубликована: Дек. 26, 2019
The
global
rise
in
obesity
and
steady
decline
sperm
quality
are
two
alarming
trends
that
have
emerged
during
recent
decades.
In
parallel,
evidence
from
model
organisms
shows
paternal
diet
can
affect
offspring
metabolic
health
a
process
involving
tRNA-derived
small
RNA
(tsRNA).
Here,
we
report
human
acutely
sensitive
to
nutrient
flux,
both
terms
of
motility
changes
tsRNA.
Over
the
course
2-week
intervention,
which
first
introduced
healthy
followed
by
rich
sugar,
increased
stabilized
at
high
levels.
Small
RNA-seq
on
repeatedly
sampled
same
individuals
revealed
tsRNAs
were
up-regulated
eating
high-sugar
for
just
1
week.
Unsupervised
clustering
identified
independent
pathways
biogenesis
these
tsRNAs:
one
novel
class
fragments
with
specific
cleavage
T-loop
mature
nuclear
tRNAs
other
exclusively
mitochondrial
tsRNAs.
Mitochondrial
involvement
was
further
supported
similar
up-regulation
rRNA-derived
(rsRNA).
Notably,
sugar-sensitive
tsRNA
positively
associated
simultaneous
negatively
an
clinical
cohort.
This
rapid
response
dietary
intervention
is
attuned
intergenerational
responses
found
organisms.
More
importantly,
our
findings
suggest
shared
diet-sensitive
mechanisms
between
tsRNA,
provide
insights
about
interplay
nutrition
male
reproductive
health.
Genome Research,
Год журнала:
2018,
Номер
28(4), С. 519 - 531
Опубликована: Март 12, 2018
Eukaryotic
centromeres
contain
the
kinetochore,
which
connects
chromosomes
to
spindle
allowing
segregation.
During
meiosis,
are
suppressed
for
inter-homolog
crossover,
as
recombination
in
these
regions
can
cause
chromosome
missegregation
and
aneuploidy.
Plant
surrounded
by
transposon-dense
pericentromeric
heterochromatin
that
is
epigenetically
silenced
histone
3
lysine
9
dimethylation
(H3K9me2),
DNA
methylation
CG
non-CG
sequence
contexts.
However,
role
of
chromatin
modifications
control
meiotic
pericentromeres
not
fully
understood.
Here,
we
show
disruption
Arabidopsis
thaliana
H3K9me2
pathways,
example,
via
mutation
H3K9
methyltransferase
genes
KYP/SUVH4
SUVH5
SUVH6,
or
CHG
gene
CMT3,
increases
proximity
centromeres.
Using
immunocytological
detection
MLH1
foci
genotyping
sequencing
recombinant
plants,
observe
pathway
mutants
increased
crossovers.
Increased
H3K9me2/non-CG
occurs
hybrid
inbred
backgrounds
likely
involves
contributions
from
both
interfering
noninterfering
crossover
repair
pathways.
We
also
double-strand
breaks
(DSBs)
increase
within
pericentromeres,
purification
SPO11-1-oligonucleotides.
Therefore,
exert
a
repressive
effect
on
DSB
formation
plant
heterochromatin.
Our
results
may
account
selection
enhancer
trap
Dissociation
(Ds)
transposons
into
CMT3
with
proximal
transposon
launch-pads.
Cold Spring Harbor Perspectives in Biology,
Год журнала:
2013,
Номер
5(10), С. a017921 - a017921
Опубликована: Окт. 1, 2013
Rodolfo
Aramayo1
and
Eric
U.
Selker2
1Department
of
Biology,
Texas
A&M
University,
College
Station,
77843-3258
2Department
Biology
Institute
Molecular
University
Oregon,
Eugene,
Oregon
97403-1229
Correspondence:
selker{at}uoregon.edu
Cold Spring Harbor Perspectives in Biology,
Год журнала:
2016,
Номер
8(5), С. a019372 - a019372
Опубликована: Май 1, 2016
Geneviève
Almouzni1
and
Howard
Cedar2
1Department
of
Nuclear
Dynamics
Genome
Plasticity,
Institut
Curie,
Section
de
recherche,
75231
Paris
Cedex
05,
France
2Department
Developmental
Biology
Cancer
Research,
Institute
for
Medical
Research
Israel–Canada,
Hebrew
University
School,
Ein
Kerem,
Jerusalem,
Israel
91120
Correspondence:
genevieve.almouzni{at}curie.fr
