Pathways of N2O production by marine ammonia-oxidizing archaea determined from dual-isotope labeling DOI Creative Commons
Xianhui Wan, Lei Hou, Shuh‐Ji Kao

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2023, Номер 120(11)

Опубликована: Март 8, 2023

The ocean is a net source of the greenhouse gas and ozone-depleting substance, nitrous oxide (N2O), to atmosphere. Most that N2O produced as trace side product during ammonia oxidation, primarily by ammonia-oxidizing archaea (AOA), which numerically dominate community in most marine environments. pathways production their kinetics, however, are not completely understood. Here, we use 15N 18O isotopes determine kinetics nitrogen (N) oxygen (O) atoms model AOA species, Nitrosopumilus maritimus. We find apparent half saturation constants nitrite comparable, suggesting both processes enzymatically controlled tightly coupled at low concentrations. constituent derived from ammonia, nitrite, O2, H2O via multiple pathways. Ammonia primary N N2O, but its contribution varies with ratio. ratio 45N2O 46N2O (i.e., single or double labeled N) substrate ratio, leading widely varying isotopic signatures pool. O2 for O atoms. In addition previously demonstrated hybrid formation pathway, found substantial hydroxylamine while reduction an insignificant N2O. Our study highlights power dual 15N-18O isotope labeling disentangle microbes, implications interpretation regulation sources.

Язык: Английский

Nitrification and denitrification in recirculating aquaculture systems: the processes and players DOI

P. Geetha,

V. J. Rejish Kumar,

I.S. Bright Singh

и другие.

Reviews in Aquaculture, Год журнала: 2021, Номер 13(4), С. 2053 - 2075

Опубликована: Март 22, 2021

Abstract One of the major challenges in any sustainable aquaculture production systems is accumulation nitrogenous waste such as ammonia and its biological nitrification products vi z nitrite nitrate. Considering bio‐security issues, amelioration these wastes without water exchange can be accomplished only by way establishing situ denitrification through biofilters/bioreactors activated with nitrifying/denitrifying bioaugmentors. In systems, coexistence aerobic denitrifiers, anaerobic oxidizers (Anammox) complete (Comammox) together autotrophic nitrifiers enhance coupled nitrification–denitrification. This promotes total nitrogen removal external carbon supplements or additional anerobic compartment system. Various recirculating (RAS) comprise diverse nitrifying community thereby imparting distinctive conversion Meanwhile, structure population dynamics consortia are influenced environmental factors forming decisive success processes. Accordingly, understanding complexity composition turns out to a requirement facilitate improvised performance. this context, review addresses different significance nitrification–denitrification RAS, genetic diversity key players methods analysis their structure, current application prospects processes RAS.

Язык: Английский

Процитировано

91
N2O and NOy production by the comammox bacterium Nitrospira inopinata in comparison with canonical ammonia oxidizers DOI
Ping Han, Dianming Wu, Dongyao Sun

и другие.

Water Research, Год журнала: 2020, Номер 190, С. 116728 - 116728

Опубликована: Дек. 4, 2020

Язык: Английский

Процитировано

86
NosZ clade II rather than clade I determine in situ N2O emissions with different fertilizer types under simulated climate change and its legacy DOI
Xiaoya Xu, Yaowei Liu, Bhupinder Pal Singh

и другие.

Soil Biology and Biochemistry, Год журнала: 2020, Номер 150, С. 107974 - 107974

Опубликована: Сен. 11, 2020

Язык: Английский

Процитировано

84
Niche differentiation of clade A comammox Nitrospira and canonical ammonia oxidizers in selected forest soils DOI
Chaoyu Li, Hang‐Wei Hu, Qing‐Lin Chen

и другие.

Soil Biology and Biochemistry, Год журнала: 2020, Номер 149, С. 107925 - 107925

Опубликована: Июль 28, 2020

Язык: Английский

Процитировано

79
Ammonia-oxidizing archaea are integral to nitrogen cycling in a highly fertile agricultural soil DOI Creative Commons
Laibin Huang,

Seemanti Chakrabarti,

Jennifer Cooper

и другие.

ISME Communications, Год журнала: 2021, Номер 1(1)

Опубликована: Июнь 1, 2021

Nitrification is a central process in the global nitrogen cycle, carried out by complex network of ammonia-oxidizing archaea (AOA), bacteria (AOB), complete (comammox) bacteria, and nitrite-oxidizing (NOB). responsible for significant leaching N2O emissions thought to impede plant use efficiency agricultural systems. However, actual contribution each nitrifier group net rates remain poorly understood. We hypothesized that highly fertile soils with high organic matter mineralization could allow detailed characterization N cycling these soils. Using combination molecular activity measurements, we show mixed AOA, AOB, comammox community, AOA outnumbered low diversity assemblages AOB 50- 430-fold, strongly dominated nitrification activities yields between 0.18 0.41 ng N2O-N per µg NOx-N cropped, fallow, as well native soil. were not significantly different plant-covered fallow plots. Mass balance calculations indicated plants relied heavily on nitrate, ammonium primary source Together, results imply integral part cycle

Язык: Английский

Процитировано

74
Stimulation of ammonia oxidizer and denitrifier abundances by nitrogen loading: Poor predictability for increased soil N2O emission DOI
Yong Zhang, Feng Zhang, Diego Ábalos

и другие.

Global Change Biology, Год журнала: 2021, Номер 28(6), С. 2158 - 2168

Опубликована: Дек. 19, 2021

Unprecedented nitrogen (N) inputs into terrestrial ecosystems have profoundly altered soil N cycling. Ammonia oxidizers and denitrifiers are the main producers of nitrous oxide (N2 O), but it remains unclear how ammonia oxidizer denitrifier abundances will respond to loading whether their responses can predict N-induced changes in N2 O emission. By synthesizing 101 field studies worldwide, we showed that significantly increased abundance by 107% 45%. The increases both were primarily explained form, more specifically, organic had stronger effects on than mineral loading. Nitrogen emission 261%, whereas there was no clear relationship between shifts abundances. Our field-based results challenge laboratory-based hypothesis would directly cause higher Instead, key abiotic factors (mean annual precipitation, pH, C:N ratio, ecosystem type) Altogether, these findings highlight need for considering roles regulating transformations under better understand microbially mediated

Язык: Английский

Процитировано

74
Ammonia-oxidizing bacteria rather than ammonia-oxidizing archaea dominate nitrification in a nitrogen-fertilized calcareous soil DOI

Wenxin Zou,

Ming Lang,

Ling Zhang

и другие.

The Science of The Total Environment, Год журнала: 2021, Номер 811, С. 151402 - 151402

Опубликована: Ноя. 3, 2021

Язык: Английский

Процитировано

73
A critical review on microbial ecology in the novel biological nitrogen removal process: Dynamic balance of complex functional microbes for nitrogen removal DOI
Hong Chen, Ke Liu,

Enzhe Yang

и другие.

The Science of The Total Environment, Год журнала: 2022, Номер 857, С. 159462 - 159462

Опубликована: Окт. 17, 2022

Язык: Английский

Процитировано

68
The contribution of ammonia-oxidizing archaea and bacteria to gross nitrification under different substrate availability DOI Creative Commons
Tobias Rütting, Philipp Schleusner, Linda Hink

и другие.

Soil Biology and Biochemistry, Год журнала: 2021, Номер 160, С. 108353 - 108353

Опубликована: Июль 4, 2021

The first step of autotrophic nitrification is performed by ammonia-oxidizing archaea (AOA) and bacteria (AOB). Recent studies show that their relative contributions are determined the substrate sources availability, yet evidence provided quantification respective gross activities in soil lacking. Here, we conducted a microcosm study with agricultural high (50 μg N g−1) low (5 ammonium application, quantified rates using 15N-tracers. AOA AOB were distinguished bacterial inhibitor 1-octyne acetylene, which inhibits both AOB. Under supply, contributed equally to ammonia oxidation, but outcompeted under higher supply. These results provide direct availability affects contribution nitrification.

Язык: Английский

Процитировано

63
Dynamics of Soil Microbial N-Cycling Strategies in Response to Cadmium Stress DOI

Haochun Zhao,

Jiahui Lin, Xuehua Wang

и другие.

Environmental Science & Technology, Год журнала: 2021, Номер 55(20), С. 14305 - 14315

Опубликована: Окт. 7, 2021

Globally increasing trace metal contamination of soils requires a better mechanistic understanding metal-stress impacts on microbially mediated nutrient cycling. Herein, 5-month laboratory experiment was employed to assess the effects cadmium (Cd) soil microbial N-cycling processes and associated functional gene abundance, with without urea amendment. In non-N-amended soils, Cd progressively stimulated populations for N acquisition from initial dissolved organic (DON) later recalcitrant N. The acceleration catabolism synchronously coupled C resulting in increased CO2/N2O fluxes adenosine triphosphate (ATP) contents. abundance microbes deemed inefficient gradually repressed after an stimulation period. We posit that enhanced exergonic diminished need endergonic activities as survival strategy communities experiencing stress. With amendment, exhibited effect nitrification promotion mineralization, along increase populations. N-amended accelerated N/C transformation processes, but decreased N2O CO2 by 19 14%, respectively. This implies under eutrophic conditions, altered C/N metabolism dominance catabolic anabolic processes. These results infer nutrient-based adjustment strategies enhance their resistance.

Язык: Английский

Процитировано

62