Pathways of N2O production by marine ammonia-oxidizing archaea determined from dual-isotope labeling DOI Creative Commons
Xianhui Wan, Lei Hou, Shuh‐Ji Kao

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(11)

Published: March 8, 2023

The ocean is a net source of the greenhouse gas and ozone-depleting substance, nitrous oxide (N2O), to atmosphere. Most that N2O produced as trace side product during ammonia oxidation, primarily by ammonia-oxidizing archaea (AOA), which numerically dominate community in most marine environments. pathways production their kinetics, however, are not completely understood. Here, we use 15N 18O isotopes determine kinetics nitrogen (N) oxygen (O) atoms model AOA species, Nitrosopumilus maritimus. We find apparent half saturation constants nitrite comparable, suggesting both processes enzymatically controlled tightly coupled at low concentrations. constituent derived from ammonia, nitrite, O2, H2O via multiple pathways. Ammonia primary N N2O, but its contribution varies with ratio. ratio 45N2O 46N2O (i.e., single or double labeled N) substrate ratio, leading widely varying isotopic signatures pool. O2 for O atoms. In addition previously demonstrated hybrid formation pathway, found substantial hydroxylamine while reduction an insignificant N2O. Our study highlights power dual 15N-18O isotope labeling disentangle microbes, implications interpretation regulation sources.

Language: Английский

Nitrification and denitrification in recirculating aquaculture systems: the processes and players DOI

P. Geetha,

V. J. Rejish Kumar,

I.S. Bright Singh

et al.

Reviews in Aquaculture, Journal Year: 2021, Volume and Issue: 13(4), P. 2053 - 2075

Published: March 22, 2021

Abstract One of the major challenges in any sustainable aquaculture production systems is accumulation nitrogenous waste such as ammonia and its biological nitrification products vi z nitrite nitrate. Considering bio‐security issues, amelioration these wastes without water exchange can be accomplished only by way establishing situ denitrification through biofilters/bioreactors activated with nitrifying/denitrifying bioaugmentors. In systems, coexistence aerobic denitrifiers, anaerobic oxidizers (Anammox) complete (Comammox) together autotrophic nitrifiers enhance coupled nitrification–denitrification. This promotes total nitrogen removal external carbon supplements or additional anerobic compartment system. Various recirculating (RAS) comprise diverse nitrifying community thereby imparting distinctive conversion Meanwhile, structure population dynamics consortia are influenced environmental factors forming decisive success processes. Accordingly, understanding complexity composition turns out to a requirement facilitate improvised performance. this context, review addresses different significance nitrification–denitrification RAS, genetic diversity key players methods analysis their structure, current application prospects processes RAS.

Language: Английский

Citations

91
N2O and NOy production by the comammox bacterium Nitrospira inopinata in comparison with canonical ammonia oxidizers DOI
Ping Han, Dianming Wu, Dongyao Sun

et al.

Water Research, Journal Year: 2020, Volume and Issue: 190, P. 116728 - 116728

Published: Dec. 4, 2020

Language: Английский

Citations

86
NosZ clade II rather than clade I determine in situ N2O emissions with different fertilizer types under simulated climate change and its legacy DOI
Xiaoya Xu, Yaowei Liu, Bhupinder Pal Singh

et al.

Soil Biology and Biochemistry, Journal Year: 2020, Volume and Issue: 150, P. 107974 - 107974

Published: Sept. 11, 2020

Language: Английский

Citations

84
Niche differentiation of clade A comammox Nitrospira and canonical ammonia oxidizers in selected forest soils DOI
Chaoyu Li, Hang‐Wei Hu, Qing‐Lin Chen

et al.

Soil Biology and Biochemistry, Journal Year: 2020, Volume and Issue: 149, P. 107925 - 107925

Published: July 28, 2020

Language: Английский

Citations

79
Ammonia-oxidizing archaea are integral to nitrogen cycling in a highly fertile agricultural soil DOI Creative Commons
Laibin Huang,

Seemanti Chakrabarti,

Jennifer Cooper

et al.

ISME Communications, Journal Year: 2021, Volume and Issue: 1(1)

Published: June 1, 2021

Nitrification is a central process in the global nitrogen cycle, carried out by complex network of ammonia-oxidizing archaea (AOA), bacteria (AOB), complete (comammox) bacteria, and nitrite-oxidizing (NOB). responsible for significant leaching N2O emissions thought to impede plant use efficiency agricultural systems. However, actual contribution each nitrifier group net rates remain poorly understood. We hypothesized that highly fertile soils with high organic matter mineralization could allow detailed characterization N cycling these soils. Using combination molecular activity measurements, we show mixed AOA, AOB, comammox community, AOA outnumbered low diversity assemblages AOB 50- 430-fold, strongly dominated nitrification activities yields between 0.18 0.41 ng N2O-N per µg NOx-N cropped, fallow, as well native soil. were not significantly different plant-covered fallow plots. Mass balance calculations indicated plants relied heavily on nitrate, ammonium primary source Together, results imply integral part cycle

Language: Английский

Citations

74
Stimulation of ammonia oxidizer and denitrifier abundances by nitrogen loading: Poor predictability for increased soil N2O emission DOI
Yong Zhang, Feng Zhang, Diego Ábalos

et al.

Global Change Biology, Journal Year: 2021, Volume and Issue: 28(6), P. 2158 - 2168

Published: Dec. 19, 2021

Unprecedented nitrogen (N) inputs into terrestrial ecosystems have profoundly altered soil N cycling. Ammonia oxidizers and denitrifiers are the main producers of nitrous oxide (N2 O), but it remains unclear how ammonia oxidizer denitrifier abundances will respond to loading whether their responses can predict N-induced changes in N2 O emission. By synthesizing 101 field studies worldwide, we showed that significantly increased abundance by 107% 45%. The increases both were primarily explained form, more specifically, organic had stronger effects on than mineral loading. Nitrogen emission 261%, whereas there was no clear relationship between shifts abundances. Our field-based results challenge laboratory-based hypothesis would directly cause higher Instead, key abiotic factors (mean annual precipitation, pH, C:N ratio, ecosystem type) Altogether, these findings highlight need for considering roles regulating transformations under better understand microbially mediated

Language: Английский

Citations

74
Ammonia-oxidizing bacteria rather than ammonia-oxidizing archaea dominate nitrification in a nitrogen-fertilized calcareous soil DOI

Wenxin Zou,

Ming Lang,

Ling Zhang

et al.

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 811, P. 151402 - 151402

Published: Nov. 3, 2021

Language: Английский

Citations

73
A critical review on microbial ecology in the novel biological nitrogen removal process: Dynamic balance of complex functional microbes for nitrogen removal DOI
Hong Chen, Ke Liu,

Enzhe Yang

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 857, P. 159462 - 159462

Published: Oct. 17, 2022

Language: Английский

Citations

68
The contribution of ammonia-oxidizing archaea and bacteria to gross nitrification under different substrate availability DOI Creative Commons
Tobias Rütting, Philipp Schleusner, Linda Hink

et al.

Soil Biology and Biochemistry, Journal Year: 2021, Volume and Issue: 160, P. 108353 - 108353

Published: July 4, 2021

The first step of autotrophic nitrification is performed by ammonia-oxidizing archaea (AOA) and bacteria (AOB). Recent studies show that their relative contributions are determined the substrate sources availability, yet evidence provided quantification respective gross activities in soil lacking. Here, we conducted a microcosm study with agricultural high (50 μg N g−1) low (5 ammonium application, quantified rates using 15N-tracers. AOA AOB were distinguished bacterial inhibitor 1-octyne acetylene, which inhibits both AOB. Under supply, contributed equally to ammonia oxidation, but outcompeted under higher supply. These results provide direct availability affects contribution nitrification.

Language: Английский

Citations

63
Dynamics of Soil Microbial N-Cycling Strategies in Response to Cadmium Stress DOI

Haochun Zhao,

Jiahui Lin, Xuehua Wang

et al.

Environmental Science & Technology, Journal Year: 2021, Volume and Issue: 55(20), P. 14305 - 14315

Published: Oct. 7, 2021

Globally increasing trace metal contamination of soils requires a better mechanistic understanding metal-stress impacts on microbially mediated nutrient cycling. Herein, 5-month laboratory experiment was employed to assess the effects cadmium (Cd) soil microbial N-cycling processes and associated functional gene abundance, with without urea amendment. In non-N-amended soils, Cd progressively stimulated populations for N acquisition from initial dissolved organic (DON) later recalcitrant N. The acceleration catabolism synchronously coupled C resulting in increased CO2/N2O fluxes adenosine triphosphate (ATP) contents. abundance microbes deemed inefficient gradually repressed after an stimulation period. We posit that enhanced exergonic diminished need endergonic activities as survival strategy communities experiencing stress. With amendment, exhibited effect nitrification promotion mineralization, along increase populations. N-amended accelerated N/C transformation processes, but decreased N2O CO2 by 19 14%, respectively. This implies under eutrophic conditions, altered C/N metabolism dominance catabolic anabolic processes. These results infer nutrient-based adjustment strategies enhance their resistance.

Language: Английский

Citations

62