Mass Spectrometry Reviews,
Год журнала:
2021,
Номер
42(1), С. 131 - 143
Опубликована: Июнь 18, 2021
Abstract
In
recent
years,
metabolomics
has
emerged
as
a
pivotal
approach
for
the
holistic
analysis
of
metabolites
in
biological
systems.
The
rapid
progress
analytical
equipment,
coupled
to
rise
powerful
data
processing
tools,
now
provides
unprecedented
opportunities
deepen
our
understanding
relationships
between
biochemical
processes
and
physiological
or
phenotypic
conditions
living
organisms.
However,
obtain
unbiased
coverage
hundreds
thousands
remains
challenging
task.
Among
panel
available
methods,
targeted
untargeted
mass
spectrometry
approaches
are
among
most
commonly
used.
While
usually
relies
on
multiple‐reaction
monitoring
acquisition,
use
either
data‐independent
acquisition
(DIA)
data‐dependent
(DDA)
methods.
Unlike
DIA,
DDA
offers
possibility
get
real,
selective
MS/MS
spectra
thus
improve
metabolite
assignment
when
performing
metabolomics.
Yet,
settings
more
complex
establish
than
DIA
settings,
result,
is
prone
errors
method
development
application.
Here,
we
present
tutorial
which
guidelines
how
optimize
technical
parameters
essential
proper
experiments
applications.
This
organized
series
rules
describing
impact
different
quality.
It
primarily
intended
users
spectrometrists
that
wish
acquire
both
theoretical
background
practical
tips
developing
effective
Critical Reviews in Food Science and Nutrition,
Год журнала:
2020,
Номер
61(9), С. 1448 - 1469
Опубликована: Май 22, 2020
As
one
of
the
omics
fields,
metabolomics
has
unique
advantages
in
facilitating
understanding
physiological
and
pathological
activities
biology,
physiology,
pathology,
food
science.
In
this
review,
based
on
developments
analytical
chemistry
tools,
cheminformatics,
bioinformatics
methods,
we
highlight
current
applications
safety,
authenticity
quality,
traceability.
Additionally,
combined
use
with
other
techniques
for
"foodomics"
is
comprehensively
described.
Finally,
latest
advances,
practical
challenges
limitations,
requirements
related
to
application
are
critically
discussed,
providing
new
insight
into
analysis.
Metabolites,
Год журнала:
2020,
Номер
10(1), С. 37 - 37
Опубликована: Янв. 15, 2020
Plant-derived
natural
products
have
long
been
considered
a
valuable
source
of
lead
compounds
for
drug
development.
Natural
extracts
are
usually
composed
hundreds
to
thousands
metabolites,
whereby
the
bioactivity
can
be
represented
by
synergism
between
several
metabolites.
However,
isolating
every
single
compound
from
extract
is
not
always
possible
due
complex
chemistry
and
presence
most
secondary
metabolites
at
very
low
levels.
Metabolomics
has
emerged
in
recent
years
as
an
indispensable
tool
analysis
crude
extracts,
leading
paradigm
shift
research.
Analytical
methods
such
mass
spectrometry
(MS)
nuclear
magnetic
resonance
(NMR)
used
comprehensively
annotate
constituents
plant
screening,
discovery
well
quality
control
purposes
those
required
phytomedicine.
In
this
review,
current
advancements
sample
preparation,
measurements,
data
presented
alongside
few
case
studies
successful
applications
these
processes
product
discovery.
Phytochemistry,
Год журнала:
2020,
Номер
174, С. 112347 - 112347
Опубликована: Апрель 25, 2020
Over
8000
different
flavonoids
have
been
described
and
a
considerable
number
of
new
flavonoid
structures
are
being
elucidated
every
year.
The
advent
metabolomics
alongside
the
development
phytochemical
genetics
–
wherein
genetic
basis
underlying
regulation
levels
plant
metabolites
is
determined
has
provided
massive
boost
to
such
efforts.
That
said
our
understanding
individual
function(s)
vast
majority
that
constitute
this
important
class
phytochemicals
remains
unknown.
Here
we
review
what
known
concerning
major
decorative
modifications
in
plants,
namely
hydroxylation,
glycosylation,
methylation
acylation.
Our
focus
with
regard
planta
function
these
modified
compounds,
however,
also
highlight
demonstrated
bioactive
roles
which
they
possess.
We
additionally
performed
comprehensive
survey
listed
KNApSAcK
database
order
assess
frequency
occurrence
each
type
modification.
conclude
whilst
research
carried
out
regarding
biological
most
studies
date
merely
information
on
compound
or
sub-classes
thereof
as
whole
too
little
currently
specific
role
metabolites.
We,
therefore,
finally
suggest
framework
based
available
tools
by
relative
importance
compounds
can
be
assessed
under
various
conditions
fill
knowledge-gap.
