Genome-wide identification and characterization of NBLRR genes in finger millet (Eleusine coracana L.) and their expression in response to Magnaporthe grisea infection

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Янв. 29, 2024

Abstract Background The nucleotide binding site leucine rich repeat (NBLRR) genes significantly regulate defences against phytopathogens in plants. genome-wide identification and analysis of NBLRR have been performed several species. However, the detailed evolution, structure, expression s functional response to Magnaporthe grisea are unknown finger millet ( Eleusine coracana (L.) Gaertn.). Results scanning genome resulted 116 EcNBLRRs1-116 ) encompassing 64 CC-NB-LRR, 47 NB-LRR 5 CC R -NB-LRR types. evolutionary studies among NBLRRs five Gramineae species, viz., purple false brome Brachypodium distachyon P.Beauv.), E. ), rice Oryza sativa L.), sorghum Sorghum bicolor L. (Moench)) foxtail Setaria italica P.Beauv.) showed evolution ancestral lineage target species subsequent divergence through gene-loss events. purifying selection (Ka/Ks < 1) shaped expansions paralogs orthologs promoter sequence various stress- phytohormone-responsive cis -acting elements besides growth development, indicating their potential role disease defence regulatory mechanisms. 22 EcNBLRRs genotypes showing contrasting responses infection revealed four early late stages, respectively. six these nine candidate proteins, viz ., EcNBLRR21, EcNBLRR26, EcNBLRR30, EcNBLRR45, EcNBLRR55 EcNBLRR76 CC, NB LRR domains, whereas EcNBLRR23, EcNBLRR32 EcNBLRR83 somains. Conclusion EcNBLRR assigning blast resistance millet. These results pave foundation for in-depth targeted editing transgenic approaches.

Язык: Английский

---

Genome engineering of disease susceptibility genes for enhancing resistance in plants

Functional & Integrative Genomics, Год журнала: 2023, Номер 23(3)

Опубликована: Июнь 20, 2023

Язык: Английский

---

Telomere-to-telomere and gap-free genome assembly of a susceptible grapevine species (Thompson Seedless) to facilitate grape functional genomics

Horticulture Research, Год журнала: 2023, Номер 11(1)

Опубликована: Дек. 13, 2023

Abstract Grapes are globally recognized as economically significant fruit trees. Among grape varieties, Thompson Seedless holds paramount influence for fresh consumption and extensive applications in winemaking, drying, juicing. This variety is one of the most efficient genotypes genetic modification. However, lack a high-quality genome has impeded effective breeding efforts. Here, we present reference with all 19 chromosomes represented contiguous sequences (N50 = 27.1 Mb) zero gaps prediction telomeres centromeres. Compared previous assembly (TSv1 version), new incorporates an additional 31.5 Mb sequenced data annotation total 30 397 protein-coding genes. We also performed meticulous analysis to identify nucleotide-binding leucine-rich repeat genes (NLRs) two wild varieties renowned their disease resistance. Our revealed reduction number types NLRs, TIR-NB-LRR (TNL) CC-NB-LRR (CNL), Seedless, which may have led its sensitivity many fungal diseases, such powdery mildew, increase third type, RPW8 (resistance mildew 8)-NB-LRR (RNL). Subsequently, transcriptome showed enrichment NLRs during infection, emphasizing pivotal role these elements grapevine’s defense against mildew. The successful significantly contributes genomics research, providing insight into importance seedlessness, resistance, color traits, can be used facilitate molecular

Язык: Английский

---

Molecular Mechanisms Underlying Potential Pathogen Resistance in Cannabis sativa

Plants, Год журнала: 2023, Номер 12(15), С. 2764 - 2764

Опубликована: Июль 25, 2023

Cannabis (Cannabis sativa L.) is one of the earliest cultivated crops, valued for producing a broad spectrum compounds used in medicinal products and being source food fibre. Despite availability its genome sequences, few studies explore molecular mechanisms involved pathogen defense, underlying biological pathways are poorly defined places. Here, we provide an overview defence responses against common pathogens, such as Golovinomyces spp., Fusarium Botrytis cinerea Pythium spp. For each these after summary their characteristics symptoms, identifying genes resistance mechanisms. Many focus on potential involvement disease-resistance genes, while others refer to other plants however whose results may be use research. Omics investigations allowing identification candidate highlighted, editing approaches generate resistant species based CRISPR/Cas9 technology discussed. According emerging results, model including both immune plant–pathogen interactions finally proposed. To our knowledge, this first review Cannabis.

Язык: Английский

---

Deep R-gene discovery in HLB resistant wild Australian limes uncovers evolutionary features and potentially important loci for hybrid breeding

Frontiers in Plant Science, Год журнала: 2025, Номер 15

Опубликована: Янв. 29, 2025

Huanglongbing (HLB) is a devastating citrus disease that threatens the industry worldwide. HLB associated with bacteria Candidatus Liberibacter asiaticus (CLas) and as of today, there are no tools for economically viable management. Several wild Australian limes have been identified to be resistant their resistance hypothesized conferred by genes (R-genes), which mediate pathogen-specific defense responses. The aim this study was gain insight into genomic features R-genes in limes, comparison susceptible cultivars. In study, we used five genomes, including three ( Citrus australasica , C. glauca inodora ) two cultivated species clementina sinensis ). Our results indicate up 70% were unannotated regions original genome annotation each species, owing use R-gene specific pipeline. Surprisingly, harbored 15.8 104% more than limes. all over 75% occurred clusters nearly 80% concentrated chromosomes (Chr3, 5 7). syntenic based phylogenic classification grouped according HLB-resistance levels, reflecting association between these distinct origins. Domain structure analysis revealed substantial similarities citrus. Investigation chromosomal sites underlying R diversifying selection signatures on several regions. findings will aid development genome-assisted breeding HLB-resistant varieties.

Язык: Английский

---

PlantNLRatlas: a comprehensive dataset of full- and partial-length NLR resistance genes across 100 chromosome-level plant genomes

Frontiers in Plant Science, Год журнала: 2023, Номер 14

Опубликована: Апрель 14, 2023

Plants have evolved two layers of protection against biotic stress: PAMP-triggered immunity (PTI) and effector-triggered (ETI). The primary mechanism ETI involves nucleotide-binding leucine-rich repeat immune receptors (NLRs). Although NLR genes been studied in several plant species, a comprehensive database NLRs across diverse array species is still lacking. Here, we present thorough analysis 100 high-quality genomes (PlantNLRatlas). PlantNLRatlas includes total 68,452 NLRs, which 3,689 are full-length 64,763 partial-length NLRs. majority groups were phyletically clustered. In addition, the domain sequences found to be highly conserved within each group. Our dataset complementary RefPlantNLR, collection experimentally confirmed. should prove helpful for comparative investigations range groups, including understudied taxa. Finally, resource intended help field move past monolithic understanding structure function.

Язык: Английский

---

NLR- and mlo-Based Resistance Mechanisms against Powdery Mildew in Cannabis sativa

Plants, Год журнала: 2023, Номер 13(1), С. 105 - 105

Опубликована: Дек. 29, 2023

Powdery mildew (PM) is one of the most common Cannabis sativa diseases. In spite this, very few documented studies have characterized resistance genes involved in PM defense mechanisms, or sources natural genetic cannabis. The focus present work on two primary mechanisms for qualitative against PM. first based (R) by conserved nucleotide-binding site and/or leucine-rich repeat domains (NLRs). second involves susceptibility (S) genes, and particularly locus o (MLO) whose loss-of-function mutations seem to be a reliable way protect plants from infection. defenses are thus discussed, mainly detailing strategies these mechanisms. Emerging about this research topic also reported and, significant results, potential model cannabis plant–pathogen interactions proposed. Finally, innovative approaches, pyramiding multiple R as well engineering genome editing methods knocking out S obtain durable PM-resistant cultivars with broad-spectrum range.

Язык: Английский

---

A landscape of resistance gene analogs in sour cherry (Prunus cerasus L.)

BMC Research Notes, Год журнала: 2024, Номер 17(1)

Опубликована: Окт. 6, 2024

Abstract Objective This research aims to analyze the presence and distribution of resistance genes in avium fruticosa subgenomes Prunus cerasus through computational methods bioinformatics tools. Results Analysis genome transcriptome sequencing data revealed a total 19,570 transcripts with at least one gene domain subgenome 19,142 . Key findings include identification 804 “complete” 817 , distinct distributions classes observed between subgenomes. Phylogenetic analysis showed clustering genes, unique proteins were identified each subgenome. Functional annotation comparisons Arabidopsis thaliana highlighted shared emphasizing complexity disease cherry species. Additionally, higher diversity RLKs RLPs was observed, 504 18 showing similarity known reference genes.

Язык: Английский

---

Rust resistance S3 loci in Coffea spp.

Physiological and Molecular Plant Pathology, Год журнала: 2023, Номер 127, С. 102111 - 102111

Опубликована: Авг. 11, 2023

Язык: Английский

---

Engineering effector‐triggered immunity in rice: Obstacles and perspectives

Plant Cell & Environment, Год журнала: 2022, Номер 46(4), С. 1143 - 1156

Опубликована: Окт. 28, 2022

Improving rice immunity is one of the most effective approaches to reduce yield loss by biotic factors, with aim increasing production 2050 amidst limited natural resources. Triggering a fast and strong immune response pathogens, effector-triggered (ETI) has intrigued scientists intensively study utilize mechanisms for engineering highly resistant plants. The conservation ETI components across species enables use generate broad-spectrum resistance in Numerous efforts have been made introduce new (R) genes, widen effector recognition spectrum on-demand R genes. Although plant still associated multiple challenges, previous attempts provided an enhanced understanding mechanisms. Here, we provide survey recent reports In addition, suggest framework future studies gene-effector interactions, including genome-scale investigations both followed structural proteins effectors, potential strategies important improve immunity.

Язык: Английский

---