NB-LRR Lineage-Specific Equipment Is Sorted Out by Sequence Pattern Adaptation and Domain Segment Shuffling DOI Open Access

Giuseppe Andolfo,

Antimo Di Donato,

Maria Raffaella Ercolano

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(22), P. 14269 - 14269

Published: Nov. 17, 2022

The nucleotide-binding and leucine-rich repeat (NB-LRR) genes, also known as resistance (R)-genes, play an important role in the activation of immune responses. In recent years, large-scale studies have been performed to highlight diversification plant NB-LRR repertories. It is well that, provide new functionalities, sequences are subject duplication, domain fusions acquisition other kinds mutations. Although some mechanisms that govern protein adaptations uncovered, retrace plant-lineage-specific evolution routes R structure, a multi-genome comparative analysis was performed. This study allowed us define groups genes sharing homology relationships across different species. worth noting most populated contained well-characterized proteins. arsenal profile such investigated five botanical families, including crop species, underline specific adaptation signatures. addition, dissection 70 NB domains R-genes revealed core motifs from which three main classes diversified. structural remodeling segments shaped repertoires observed each provided evolutionary functional insights on shuffling. Taken together, findings improved our understanding molecular adaptive selection occurring at loci.

Language: Английский

Genome-wide identification and characterization of NBLRR genes in finger millet (Eleusine coracana L.) and their expression in response to Magnaporthe grisea infection DOI Creative Commons

Alexander Balamurugan,

Mallana Gowdra Mallikarjuna,

Shilpi Bansal

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Jan. 29, 2024

Abstract Background The nucleotide binding site leucine rich repeat (NBLRR) genes significantly regulate defences against phytopathogens in plants. genome-wide identification and analysis of NBLRR have been performed several species. However, the detailed evolution, structure, expression s functional response to Magnaporthe grisea are unknown finger millet ( Eleusine coracana (L.) Gaertn.). Results scanning genome resulted 116 EcNBLRRs1-116 ) encompassing 64 CC-NB-LRR, 47 NB-LRR 5 CC R -NB-LRR types. evolutionary studies among NBLRRs five Gramineae species, viz., purple false brome Brachypodium distachyon P.Beauv.), E. ), rice Oryza sativa L.), sorghum Sorghum bicolor L. (Moench)) foxtail Setaria italica P.Beauv.) showed evolution ancestral lineage target species subsequent divergence through gene-loss events. purifying selection (Ka/Ks < 1) shaped expansions paralogs orthologs promoter sequence various stress- phytohormone-responsive cis -acting elements besides growth development, indicating their potential role disease defence regulatory mechanisms. 22 EcNBLRRs genotypes showing contrasting responses infection revealed four early late stages, respectively. six these nine candidate proteins, viz ., EcNBLRR21, EcNBLRR26, EcNBLRR30, EcNBLRR45, EcNBLRR55 EcNBLRR76 CC, NB LRR domains, whereas EcNBLRR23, EcNBLRR32 EcNBLRR83 somains. Conclusion EcNBLRR assigning blast resistance millet. These results pave foundation for in-depth targeted editing transgenic approaches.

Language: Английский

Citations

5
Genome engineering of disease susceptibility genes for enhancing resistance in plants DOI

Ritika Bishnoi,

Sehgeet Kaur,

Jagdeep Singh Sandhu

et al.

Functional & Integrative Genomics, Journal Year: 2023, Volume and Issue: 23(3)

Published: June 20, 2023

Language: Английский

Citations

12
Telomere-to-telomere and gap-free genome assembly of a susceptible grapevine species (Thompson Seedless) to facilitate grape functional genomics DOI Creative Commons
Xianhang Wang,

Mingxing Tu,

Ya Wang

et al.

Horticulture Research, Journal Year: 2023, Volume and Issue: 11(1)

Published: Dec. 13, 2023

Abstract Grapes are globally recognized as economically significant fruit trees. Among grape varieties, Thompson Seedless holds paramount influence for fresh consumption and extensive applications in winemaking, drying, juicing. This variety is one of the most efficient genotypes genetic modification. However, lack a high-quality genome has impeded effective breeding efforts. Here, we present reference with all 19 chromosomes represented contiguous sequences (N50 = 27.1 Mb) zero gaps prediction telomeres centromeres. Compared previous assembly (TSv1 version), new incorporates an additional 31.5 Mb sequenced data annotation total 30 397 protein-coding genes. We also performed meticulous analysis to identify nucleotide-binding leucine-rich repeat genes (NLRs) two wild varieties renowned their disease resistance. Our revealed reduction number types NLRs, TIR-NB-LRR (TNL) CC-NB-LRR (CNL), Seedless, which may have led its sensitivity many fungal diseases, such powdery mildew, increase third type, RPW8 (resistance mildew 8)-NB-LRR (RNL). Subsequently, transcriptome showed enrichment NLRs during infection, emphasizing pivotal role these elements grapevine’s defense against mildew. The successful significantly contributes genomics research, providing insight into importance seedlessness, resistance, color traits, can be used facilitate molecular

Language: Английский

Citations

12
Molecular Mechanisms Underlying Potential Pathogen Resistance in Cannabis sativa DOI Creative Commons
Tiziana Maria Sirangelo, Richard A. Ludlow, Natasha D. Spadafora

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(15), P. 2764 - 2764

Published: July 25, 2023

Cannabis (Cannabis sativa L.) is one of the earliest cultivated crops, valued for producing a broad spectrum compounds used in medicinal products and being source food fibre. Despite availability its genome sequences, few studies explore molecular mechanisms involved pathogen defense, underlying biological pathways are poorly defined places. Here, we provide an overview defence responses against common pathogens, such as Golovinomyces spp., Fusarium Botrytis cinerea Pythium spp. For each these after summary their characteristics symptoms, identifying genes resistance mechanisms. Many focus on potential involvement disease-resistance genes, while others refer to other plants however whose results may be use research. Omics investigations allowing identification candidate highlighted, editing approaches generate resistant species based CRISPR/Cas9 technology discussed. According emerging results, model including both immune plant–pathogen interactions finally proposed. To our knowledge, this first review Cannabis.

Language: Английский

Citations

11
Deep R-gene discovery in HLB resistant wild Australian limes uncovers evolutionary features and potentially important loci for hybrid breeding DOI Creative Commons
Jianyang Liu,

Khushwant Singh,

Matthew Huff

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 29, 2025

Huanglongbing (HLB) is a devastating citrus disease that threatens the industry worldwide. HLB associated with bacteria Candidatus Liberibacter asiaticus (CLas) and as of today, there are no tools for economically viable management. Several wild Australian limes have been identified to be resistant their resistance hypothesized conferred by genes (R-genes), which mediate pathogen-specific defense responses. The aim this study was gain insight into genomic features R-genes in limes, comparison susceptible cultivars. In study, we used five genomes, including three ( Citrus australasica , C. glauca inodora ) two cultivated species clementina sinensis ). Our results indicate up 70% were unannotated regions original genome annotation each species, owing use R-gene specific pipeline. Surprisingly, harbored 15.8 104% more than limes. all over 75% occurred clusters nearly 80% concentrated chromosomes (Chr3, 5 7). syntenic based phylogenic classification grouped according HLB-resistance levels, reflecting association between these distinct origins. Domain structure analysis revealed substantial similarities citrus. Investigation chromosomal sites underlying R diversifying selection signatures on several regions. findings will aid development genome-assisted breeding HLB-resistant varieties.

Language: Английский

Citations

0
PlantNLRatlas: a comprehensive dataset of full- and partial-length NLR resistance genes across 100 chromosome-level plant genomes DOI Creative Commons
Xiang Li,

Linna Ma,

Yingmin Wang

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: April 14, 2023

Plants have evolved two layers of protection against biotic stress: PAMP-triggered immunity (PTI) and effector-triggered (ETI). The primary mechanism ETI involves nucleotide-binding leucine-rich repeat immune receptors (NLRs). Although NLR genes been studied in several plant species, a comprehensive database NLRs across diverse array species is still lacking. Here, we present thorough analysis 100 high-quality genomes (PlantNLRatlas). PlantNLRatlas includes total 68,452 NLRs, which 3,689 are full-length 64,763 partial-length NLRs. majority groups were phyletically clustered. In addition, the domain sequences found to be highly conserved within each group. Our dataset complementary RefPlantNLR, collection experimentally confirmed. should prove helpful for comparative investigations range groups, including understudied taxa. Finally, resource intended help field move past monolithic understanding structure function.

Language: Английский

Citations

7
NLR- and mlo-Based Resistance Mechanisms against Powdery Mildew in Cannabis sativa DOI Creative Commons
Tiziana Maria Sirangelo

Plants, Journal Year: 2023, Volume and Issue: 13(1), P. 105 - 105

Published: Dec. 29, 2023

Powdery mildew (PM) is one of the most common Cannabis sativa diseases. In spite this, very few documented studies have characterized resistance genes involved in PM defense mechanisms, or sources natural genetic cannabis. The focus present work on two primary mechanisms for qualitative against PM. first based (R) by conserved nucleotide-binding site and/or leucine-rich repeat domains (NLRs). second involves susceptibility (S) genes, and particularly locus o (MLO) whose loss-of-function mutations seem to be a reliable way protect plants from infection. defenses are thus discussed, mainly detailing strategies these mechanisms. Emerging about this research topic also reported and, significant results, potential model cannabis plant–pathogen interactions proposed. Finally, innovative approaches, pyramiding multiple R as well engineering genome editing methods knocking out S obtain durable PM-resistant cultivars with broad-spectrum range.

Language: Английский

Citations

7
A landscape of resistance gene analogs in sour cherry (Prunus cerasus L.) DOI Creative Commons
Thomas Wöhner, Ofere Francis Emeriewen

BMC Research Notes, Journal Year: 2024, Volume and Issue: 17(1)

Published: Oct. 6, 2024

Abstract Objective This research aims to analyze the presence and distribution of resistance genes in avium fruticosa subgenomes Prunus cerasus through computational methods bioinformatics tools. Results Analysis genome transcriptome sequencing data revealed a total 19,570 transcripts with at least one gene domain subgenome 19,142 . Key findings include identification 804 “complete” 817 , distinct distributions classes observed between subgenomes. Phylogenetic analysis showed clustering genes, unique proteins were identified each subgenome. Functional annotation comparisons Arabidopsis thaliana highlighted shared emphasizing complexity disease cherry species. Additionally, higher diversity RLKs RLPs was observed, 504 18 showing similarity known reference genes.

Language: Английский

Citations

1
Rust resistance S3 loci in Coffea spp. DOI
P. C. da S. Ângelo, Gustavo Hiroshi Sera, Luciana Harumi Shigueoka

et al.

Physiological and Molecular Plant Pathology, Journal Year: 2023, Volume and Issue: 127, P. 102111 - 102111

Published: Aug. 11, 2023

Language: Английский

Citations

3
Engineering effector‐triggered immunity in rice: Obstacles and perspectives DOI Creative Commons
Kieu Thi Xuan Vo, Yi Qi, Jong‐Seong Jeon

et al.

Plant Cell & Environment, Journal Year: 2022, Volume and Issue: 46(4), P. 1143 - 1156

Published: Oct. 28, 2022

Improving rice immunity is one of the most effective approaches to reduce yield loss by biotic factors, with aim increasing production 2050 amidst limited natural resources. Triggering a fast and strong immune response pathogens, effector-triggered (ETI) has intrigued scientists intensively study utilize mechanisms for engineering highly resistant plants. The conservation ETI components across species enables use generate broad-spectrum resistance in Numerous efforts have been made introduce new (R) genes, widen effector recognition spectrum on-demand R genes. Although plant still associated multiple challenges, previous attempts provided an enhanced understanding mechanisms. Here, we provide survey recent reports In addition, suggest framework future studies gene-effector interactions, including genome-scale investigations both followed structural proteins effectors, potential strategies important improve immunity.

Language: Английский

Citations

5