Cellular and Molecular Life Sciences,
Год журнала:
2023,
Номер
80(8)
Опубликована: Июль 28, 2023
Abstract
Microenvironmental
factors
are
known
fundamental
regulators
of
the
phenotype
and
aggressiveness
glioblastoma
(GBM),
most
lethal
brain
tumor,
characterized
by
fast
progression
marked
resistance
to
treatments.
In
this
context,
extracellular
matrix
(ECM)
is
heavily
influence
behavior
cancer
cells
from
several
origins,
contributing
stem
cell
niches,
influencing
tumor
invasiveness
response
chemotherapy,
mediating
survival
signaling
cascades,
modulating
inflammatory
recruitment.
Here,
we
show
that
collagen
VI
(COL6),
an
ECM
protein
widely
expressed
in
both
normal
pathological
tissues,
has
a
distinctive
distribution
within
GBM
mass,
strongly
correlated
with
aggressive
phenotypically
immature
cells.
Our
data
demonstrate
COL6
sustains
stem-like
properties
supports
maintenance
transcriptional
program
promoting
proliferation
survival.
particular,
identified
specific
subset
COL6-transcriptionally
co-regulated
genes,
required
for
replicative
stress
DNA
damage,
supporting
concept
essential
stimulus
activation
through
ATM/ATR
axis.
Altogether,
these
findings
indicate
plays
pivotal
role
biology,
exerting
pleiotropic
action
across
different
hallmarks,
including
phenotypic
identity
gene
transcription,
as
well
treatments,
thus
providing
valuable
information
understanding
complex
microenvironmental
cues
underlying
malignancy.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2022,
Номер
10
Опубликована: Апрель 25, 2022
Reproduction
of
different
tissues
using
scaffolds
and
materials
is
a
major
element
in
regenerative
medicine.
The
regeneration
whole
organs
with
decellularized
extracellular
matrix
(dECM)
has
remained
goal
despite
the
use
these
for
purposes.
Recently,
decellularization
techniques
have
been
widely
used
producing
that
are
appropriate
regenerating
damaged
may
be
able
to
overcome
shortage
donor
organs.
Decellularized
ECM
offers
several
advantages
over
synthetic
compounds,
including
preserved
natural
microenvironment
features.
Different
methods
developed,
each
which
removing
cells
from
specific
under
certain
conditions.
A
variety
advanced
evaluating
process
terms
cell
removal
efficiency,
tissue
ultrastructure
preservation,
toxicity,
biocompatibility,
biodegradability,
mechanical
resistance
order
enhance
efficacy
methods.
Modification
improve
characteristics
scaffolds,
making
them
available
tissues.
Moreover,
modification
makes
options
drug
delivery,
disease
modeling,
improving
stem
growth
proliferation.
However,
considering
challenges
way
application
this
field
constantly
developing
progressively
moving
forward.
This
review
outlined
recent
sterilization
strategies,
evaluation
tests
efficient
decellularization,
processing,
application,
future
outlooks
medicine
engineering.
Journal of Tissue Engineering,
Год журнала:
2022,
Номер
13
Опубликована: Янв. 1, 2022
Decellularization
of
natural
tissues
to
produce
extracellular
matrix
is
a
promising
method
for
three-dimensional
scaffolding
and
understanding
microenvironment
the
tissue
interest.
Due
lack
universal
standard
protocol
decellularization,
recent
investigations
seek
develop
novel
methods
whole
or
partial
organ
decellularization
capable
supporting
cell
differentiation
implantation
towards
appropriate
regeneration.
This
review
provides
comprehensive
updated
perspective
on
most
advances
in
strategies
variety
organs
tissues,
highlighting
techniques
chemical,
physical,
biological,
enzymatic,
combinative-based
remove
cellular
contents
from
tissues.
In
addition,
presents
modernized
approaches
improving
protocols
numerous
types.
Cancer Research,
Год журнала:
2023,
Номер
83(9), С. 1386 - 1392
Опубликована: Янв. 13, 2023
Abstract
Collagen
is
one
of
the
most
abundant
proteins
in
animals
and
a
major
component
extracellular
matrix
(ECM)
tissues.
Besides
playing
role
as
structural
building
block
tissues,
collagens
can
modulate
behavior
cells,
their
deregulation
promote
diseases
such
cancer.
In
tumors,
many
other
ECM
molecules
are
mainly
produced
by
fibroblasts,
recent
evidence
points
toward
tumor-derived
tumor
progression
metastasis.
this
review,
we
focus
on
newly
discovered
functions
Novel
findings
have
revealed
dormancy
immune
evasion,
well
interplay
with
cancer
cell
metabolism.
Collagens
could
serve
prognostic
markers
for
patients
cancer,
therapeutic
strategies
targeting
collagen
potential
to
prevent
Frontiers in Oncology,
Год журнала:
2021,
Номер
11
Опубликована: Окт. 25, 2021
The
lack
of
traditional
cancer
treatments
has
resulted
in
an
increased
need
for
new
clinical
techniques.
Standard
two-dimensional
(2D)
models
used
to
validate
drug
efficacy
and
screening
have
a
low
FEBS Journal,
Год журнала:
2021,
Номер
289(13), С. 3603 - 3629
Опубликована: Июнь 10, 2021
Fibrosis
of
visceral
organs
such
as
the
lungs,
heart,
kidneys
and
liver
remains
a
major
cause
morbidity
mortality
is
also
associated
with
many
other
disorders,
including
cancer
metabolic
disease.
In
this
review,
we
focus
upon
microfibrillar
collagen
VI,
which
present
in
extracellular
matrix
(ECM)
most
tissues.
However,
expression
elevated
numerous
fibrotic
conditions,
idiopathic
pulmonary
disease
(IPF),
chronic
kidney
diseases.
Collagen
VI
composed
three
subunits
α1,
α2
α3,
can
be
replaced
alternate
chains
α4,
α5
or
α6.
The
C-terminal
globular
domain
(C5)
α3
proteolytically
cleaved
to
form
biologically
active
fragment
termed
endotrophin,
has
been
shown
actively
drive
fibrosis,
inflammation
insulin
resistance.
Tissue
biopsies
have
long
considered
gold
standard
for
diagnosis
monitoring
progression
identification
neoantigens
from
enzymatically
processed
revolutionised
biomarker
field,
allowing
rapid
evaluation
prognosis
well
providing
valuable
clinical
trial
endpoint
determinants.
chain
fragments
endotrophin
(PRO-C6),
C6M
C6Mα3
are
emerging
important
biomarkers
conditions.
