Novel Decellularization Method for Tissue Slices DOI Creative Commons
Maria Narciso, Anna Ulldemolins, Constança Júnior

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: March 9, 2022

Decellularization procedures have been developed and optimized for the entire organ or tissue blocks, by either perfusion of decellularizing agents through tissue's vasculature submerging large sections in solutions. However, some research aims require analysis native as well decellularized slices side side, but an optimal protocol has not yet established to address this need. Thus, main goal work was develop a fast efficient decellularization method slices-with emphasis on lung-while attached glass slide. To end, different were compared their effectiveness cellular removal while preserving extracellular matrix. The intensity DNA staining taken indicator remaining cells untreated sections. presence collagen, elastin laminin quantified using immunostaining signal quantification. Scaffolds resulting from mechanically characterized atomic force microscopy. Lung scaffolds recellularized with mesenchymal stromal assess biocompatibility. Some (CHAPS, triton, ammonia hydroxide) did achieve sufficient cell removal. Sodium dodecyl sulfate (SDS) effective (1% signal), its sharp reduction (only 6% remained) plus lower attachment ratio (32%) singled out sodium deoxycholate (SD) treatment application (6.5% due higher retention (34%) (60%). Laminin collagen fully preserved all treatments. SD also successful porcine murine (mice rat) lungs other tissues such heart, kidney, bladder. No significant mechanical differences found before after sample decellularization. acellular lung shown be biocompatible (98% survival 72 h culture). This novel decellularize opens up new methodological possibilities better understand role matrix context several diseases engineering can easily adapted scarce samples like clinical biopsies.

Language: Английский

Cell–extracellular matrix mechanotransduction in 3D DOI
Aashrith Saraswathibhatla, Dhiraj Indana, Ovijit Chaudhuri

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2023, Volume and Issue: 24(7), P. 495 - 516

Published: Feb. 27, 2023

Language: Английский

Citations

335
The role of lipids in cancer progression and metastasis DOI Creative Commons
Miguel Martín‐Pérez,

Uxue Urdiroz-Urricelqui,

Claudia Bigas

et al.

Cell Metabolism, Journal Year: 2022, Volume and Issue: 34(11), P. 1675 - 1699

Published: Oct. 18, 2022

Language: Английский

Citations

263
Decellularization in Tissue Engineering and Regenerative Medicine: Evaluation, Modification, and Application Methods DOI Creative Commons

Afarin Neishabouri,

Alireza Soltani Khaboushan, Faeze Daghigh

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: April 25, 2022

Reproduction of different tissues using scaffolds and materials is a major element in regenerative medicine. The regeneration whole organs with decellularized extracellular matrix (dECM) has remained goal despite the use these for purposes. Recently, decellularization techniques have been widely used producing that are appropriate regenerating damaged may be able to overcome shortage donor organs. Decellularized ECM offers several advantages over synthetic compounds, including preserved natural microenvironment features. Different methods developed, each which removing cells from specific under certain conditions. A variety advanced evaluating process terms cell removal efficiency, tissue ultrastructure preservation, toxicity, biocompatibility, biodegradability, mechanical resistance order enhance efficacy methods. Modification improve characteristics scaffolds, making them available tissues. Moreover, modification makes options drug delivery, disease modeling, improving stem growth proliferation. However, considering challenges way application this field constantly developing progressively moving forward. This review outlined recent sterilization strategies, evaluation tests efficient decellularization, processing, application, future outlooks medicine engineering.

Language: Английский

Citations

159
Decellularization for the retention of tissue niches DOI Creative Commons
Deana Moffat, Kaiming Ye, Sha Jin

et al.

Journal of Tissue Engineering, Journal Year: 2022, Volume and Issue: 13

Published: Jan. 1, 2022

Decellularization of natural tissues to produce extracellular matrix is a promising method for three-dimensional scaffolding and understanding microenvironment the tissue interest. Due lack universal standard protocol decellularization, recent investigations seek develop novel methods whole or partial organ decellularization capable supporting cell differentiation implantation towards appropriate regeneration. This review provides comprehensive updated perspective on most advances in strategies variety organs tissues, highlighting techniques chemical, physical, biological, enzymatic, combinative-based remove cellular contents from tissues. In addition, presents modernized approaches improving protocols numerous types.

Language: Английский

Citations

88
Tumor-associated macrophages restrict CD8+ T cell function through collagen deposition and metabolic reprogramming of the breast cancer microenvironment DOI
Kevin M. Tharp, Kelly Kersten, Ori Maller

et al.

Nature Cancer, Journal Year: 2024, Volume and Issue: 5(7), P. 1045 - 1062

Published: June 3, 2024

Language: Английский

Citations

72
Collagens in Cancer: Structural Regulators and Guardians of Cancer Progression DOI
Daniela De Martino, Jose Javier Bravo‐Cordero

Cancer Research, Journal Year: 2023, Volume and Issue: 83(9), P. 1386 - 1392

Published: Jan. 13, 2023

Abstract Collagen is one of the most abundant proteins in animals and a major component extracellular matrix (ECM) tissues. Besides playing role as structural building block tissues, collagens can modulate behavior cells, their deregulation promote diseases such cancer. In tumors, many other ECM molecules are mainly produced by fibroblasts, recent evidence points toward tumor-derived tumor progression metastasis. this review, we focus on newly discovered functions Novel findings have revealed dormancy immune evasion, well interplay with cancer cell metabolism. Collagens could serve prognostic markers for patients cancer, therapeutic strategies targeting collagen potential to prevent

Language: Английский

Citations

58
Ten Years of Extracellular Matrix Proteomics: Accomplishments, Challenges, and Future Perspectives DOI Creative Commons
Alexandra Naba

Molecular & Cellular Proteomics, Journal Year: 2023, Volume and Issue: 22(4), P. 100528 - 100528

Published: March 12, 2023

•ECM alterations cause or accompany diseases and disorders of all organ systems.•Proteomics is a method choice to profile the composition ECM tissues.•ECM proteomics can identify novel prognostic diagnostic biomarkers.•ECM uncover proteins playing functional roles in disease etiology.•Further technical advances are needed capture diversity proteoforms The extracellular matrix (ECM) complex assembly hundreds forming architectural scaffold multicellular organisms. In addition its structural role, conveys signals orchestrating cellular phenotypes. Alterations composition, abundance, structure, mechanics have been linked affecting physiological systems, including fibrosis cancer. Deciphering protein how it changes pathophysiological contexts thus first step toward understanding health development therapeutic strategies correct disease-causing alterations. Potentially, also represents vast, yet untapped reservoir biomarkers. characterized by unique biochemical properties that hindered their study: they large, heavily uniquely posttranslationally modified, highly insoluble. Overcoming these challenges, we others devised mass-spectrometry–based proteomic approaches define "matrisome," tissues. This part this review provides historical overview research presents latest now allow profiling healthy diseased second highlights recent examples illustrating has emerged as powerful discovery pipeline cancer third discusses remaining challenges limiting our ability translate findings clinical application proposes overcome them. Lastly, introduces readers resources available facilitate interpretation datasets. was once thought be impenetrable. Mass spectrometry–based proven tool decode ECM. light progress made over past decade, there reasons believe in-depth exploration matrisome within reach may soon witness translational proteomics. organisms (1Hynes R.O. evolution metazoan matrix.J. Cell Biol. 2012; 196: 671-679Crossref PubMed Scopus (177) Google Scholar, 2Adams J.C. Extracellular evolution: an overview.in: Keeley F.W. Mecham R.P. Evolution Matrix. Springer, Berlin, Heidelberg2013: 1-25https://doi.org/10.1007/978-3-642-36002-2_1Crossref 3Karamanos N.K. Theocharis A.D. Piperigkou Z. Manou D. Passi A. Skandalis S.S. et al.A guide functions matrix.FEBS J. 2021; 288: 6850-6912Crossref (34) Scholar). As such, guides cell polarization serves substrate migration, organizes cells into tissues organs, confers mechanical roles, exerts signaling through mechanotransduction (4Humphrey J.D. Dufresne E.R. Schwartz M.A. Mechanotransduction homeostasis.Nat. Rev. Mol. 2014; 15: 802-812Crossref (1185) 5Dooling L.J. Saini K. Anlaş A.A. Discher D.E. Tissue coevolves with fibrillar matrisomes fibrotic tissues.Matrix 2022; 111: 153-188Crossref (0) It cues interpreted via cell-surface receptors (e.g., integrins (6Kanchanawong P. Calderwood D.A. Organization, dynamics mechanoregulation integrin-mediated cell–ECM adhesions.Nat. 24: 142-161Crossref (7) Scholar), syndecans, adhesion GPCRs (7Liebscher I. Cevheroğlu O. Hsiao C.C. Maia A.F. Schihada H. Scholz N. GPCR research.FEBS 289: 7610-7630Crossref (5) Scholar)) orchestrate most, if not all, functions, from proliferation survival stemness differentiation. plays critical during development, growth, other processes wound healing aging (8Yamada K.M. Collins J.W. Cruz Walma Doyle Morales S.G. Lu al.Extracellular invasion tissue morphogenesis.Int. Exp. Pathol. 2019; 100: 144-152Crossref (47) 9Dzamba B.J. DeSimone D.W. sculpting embryonic tissues.Curr. Top Dev. 2018; 130: 245-274Crossref (49) 10Karamanos Neill T. Iozzo R.V. Matrix modeling remodeling: biological interplay regulating homeostasis diseases.Matrix 75–76: 1-11Crossref (156) 11Lausecker F. Lennon R. Randles M.J. kidney health, aging, disease.Kidney Int. 102: 1000-1012Abstract Full Text PDF (1) 12Ewald C.Y. longevity: systems-level approach defining matreotypes promoting aging.Gerontology. 2020; 66: 266-274Crossref (31) Simply put, essential for life. dynamic compartment undergoes compositional turnover remodeling mediated both enzymatic nonenzymatic processes. Disruption homeostasis, caused mutations genes (13Lamandé S.R. Bateman J.F. Genetic matrix.Anat. Rec. (Hoboken). 303: 1527-1542Crossref imbalance between production degradation, inadequate remodeling, results systems (14Lu Takai Weaver V.M. Werb degradation disease.Cold Spring Harb. Perspect. 2011; 3: a005058Crossref (1375) 15Bonnans C. Chou Remodelling disease.Nat. 786-801Crossref (2349) 16Theocharis Karamanos multitasking player disease.FEBS 286: 2830-2869Crossref (190) Scholar) musculoskeletal system Ehlers–Danlos syndrome (17Malfait Castori M. Francomano C.A. Giunta Kosho Byers P.H. Ehlers-Danlos syndromes.Nat. Dis. Primers. 6: 64Crossref (82) arthritis), skin scleroderma (18Schulz J.N. Plomann Sengle G. Gullberg Krieg Eckes B. New developments on - emanating control myofibroblasts.Matrix 68–69: 522-532Crossref (48) epidermolysis bullosa (19Bruckner-Tuderman L. Has Disorders cutaneous basement membrane zone--the paradigm bullosa.Matrix 33: 29-34Crossref Scholar)), cardiovascular Marfan (20Cook J.R. Carta Galatioto Ramirez Cardiovascular manifestations related diseases; multiple causing similar phenotypes.Clin. Genet. 2015; 87: 11-20Crossref (52) respiratory (lung (21Zhou Y. Horowitz Naba Ambalavanan Atabai Balestrini lung disease.Matrix 73: 77-104Crossref (138) excretory Alport syndrome, Goodpasture renal (22Bülow R.D. Boor fibrosis: more than just scaffold.J. Histochem. Cytochem. 67: 643-661Crossref (134) 23Chew Basement defects genetic diseases.Front. Pediatr. 11Crossref (50) list few. addition, excessive accumulation hallmark (24Pakshir Hinz big five macrophages, myofibroblasts, matrix, mechanics, miscommunication.Matrix 81-93Crossref (211) (25Pickup M.W. Mouw J.K. modulates hallmarks cancer.EMBO Rep. 1243-1253Crossref (1078) 26Cox T.R. cancer.Nat. Cancer. 21: 217-238Crossref (222) 27Winkler Abisoye-Ogunniyan Metcalf K.J. Concepts remodelling tumour progression metastasis.Nat. Commun. 11: 5120Crossref (584) extent deposition context cancer, assessed tumor:stroma ratio, shown value patients colorectal (28Souza da Silva R.M. Queiroga E.M. Paz A.R. Neves F.F.P. Cunha K.S. Dias E.P. Standardized assessment tumor-stroma ratio cancer: interobserver validation reproducibility potential factor.Clin. 14https://doi.org/10.1177/2632010X21989686Crossref 29van Pelt G.W. Sandberg T.P. Morreau Gelderblom van Krieken J.H.J.M. Tollenaar R.A.E.M. al.The tumour-stroma colon role impact.Histopathology. 197-206Crossref Nine 70-gene MammaPrint panel used early breast diagnosis (30Cardoso van't Veer Bogaerts Slaets Viale Delaloge S. al.70-Gene signature aid treatment decisions early-stage cancer.N. Engl. Med. 2016; 375: 717-729Crossref genes. present advantage being readily accessible, outside cells. Consequently, targeted delivery imaging agents (31Jailkhani Ingram Rashidian Rickelt Tian Mak al.Noninvasive tumor progression, metastasis, using nanobody targeting matrix.Proc. Nat. Acad. Sci. U. 116: 14181-14190Crossref 32Santimaria Moscatelli G.L. Giovannoni Neri Viti al.Immunoscintigraphic detection ED-B domain fibronectin, marker angiogenesis, cancer.Clin. Cancer Res. 2003; 9: 571-579PubMed 33Steiner Antibody-radionuclide conjugates therapy: considerations new trends.Clin. 17: 6406-6416Crossref (125) drugs, example, bispecific composed moiety recognizing disease-specific immunomodulatory cytokine (34Pasche Immunocytokines: class potent armed antibodies.Drug Discov. Today. 583-590Crossref (129) 35Lieverse R.I.Y. Van Limbergen E.J. Oberije C.J.G. Troost E.G.C. Hadrup Dingemans A.M.C. al.Stereotactic ablative body radiotherapy (SABR) combined immunotherapy (L19-IL2) versus standard care stage IV NSCLC patients, ImmunoSABR: multicentre, randomised controlled open-label phase II trial.BMC 20: 557Crossref 36Momin Mehta Bennett N.R. Ma Palmeri Chinn M.M. al.Anchoring intratumorally administered cytokines collagen safely potentiates systemic immunotherapy.Sci. Transl. 11eaaw2614Crossref (98) proposed modulating architecture biophysical ECM–cell interactions could valid various (37Nyström Bernasconi Bornert Therapies skin.Matrix 71–72: 330-347Crossref (18) 38Schuppan Ashfaq-Khan Yang A.T. Kim Y.O. Liver direct antifibrotic therapies.Matrix 435-451Crossref (244) 39Bejarano Jordāo M.J.C. Joyce J.A. Therapeutic microenvironment.Cancer 933-959Crossref (274) 40Hauge Rofstad E.K. Antifibrotic therapy normalize microenvironment.J. 18: 207Crossref (40) 41Lampi M.C. Reinhart-King Targeting stiffness attenuate disease: molecular mechanisms trials.Sci. 10eaao0475Crossref (279) 42Ley Rivera-Nieves Sandborn W.J. Shattil Integrin-based therapeutics: basis, use drugs.Nat. Drug 173-183Crossref (273) constitutes large biomarkers targets. Yet, while some elastin) families collagens, tenascins) extensively studied, whole, remained, until recently, largely underexplored (43Wilson matrix: but important proteome.Expert Proteomics. 2010; 7: 803-806Crossref (14) uncharted (44Filipe E.C. Chitty J.L. Cox Charting unexplored cancer.Int. 99: 58-76Crossref very allowing assemble capable withstanding significant stress deformations study global core, tend average 1045 amino acids long. undergo extensive intracellular posttranslational modifications (PTMs), glycosylation, lysine proline hydroxylation collagens collagen-domain-containing contribute stabilization triple-helical structure (45Rappu Salo A.M. Myllyharju Heino Role prolyl collagens.Essays Biochem. 63: 325-335Crossref glycation. higher-order structures established hydrogen bonds (46Buehler Nature designs tough collagen: explaining nanostructure fibrils.Proc. Natl. 2006; 103: 12285-12290Crossref (593) 47Shoulders M.D. Raines R.T. Collagen stability.Annu. 2009; 78: 929-958Crossref (2243) disulfide fibronectin dimers (48Schwarzbauer J.E. Fibronectins, fibrillogenesis, vivo functions.Cold 2011 Jul 1; a005041Crossref (280) covalent cross-links elastin (49Ozsvar Cain S.A. Baldock Tarakanova Weiss A.S. Tropoelastin assembly.Front. Bioeng. Biotechnol. 9643110Crossref (35) (50Ricard-Blum family.Cold a004978Crossref (1080) Scholar)). These making insoluble and, hence, challenging like SDS-PAGE, immunoprecipitation pull-down assays mass spectrometry (MS). Because high insolubility, underrepresented Further contributing underrepresentation fact that, apart few exceptions, small fraction mass. challenge comprehensive characterization broad range terms abundance. comprised abundant components, which generate many peptides (for 121 trypsin cleavage sites alpha 1 chain I), smaller secreted factors, such ECM-remodeling enzymes, growth morphogens, much lower limitation ECM, instrumentations methods fractionate peptide samples, will discussed here, key complexity different subproteomes applied (see below). attempts at ECM-rich tissues, cartilage, following enrichment employed SDS-PAGE 2D gel electrophoresis separate subsets solubilized, followed liquid chromatography coupled tandem (LC-MS/MS). studies reported up dozen proteins. At time, no feat instrumental helping shape field (51Wilson Cartilage proteomics: solutions advances.Proteomics Clin. Appl. 2008; 2: 251-263Crossref 52Lammi Häyrinen Mahonen Proteomic analysis cartilage- bone-associated samples.Electrophoresis. 27: 2687-2701Crossref 53Hattar Maller McDaniel Hansen K.C. Hedman Lyons al.Tamoxifen induces pleiotrophic mammary stroma resulting suppresses transformed phenotypes.Breast R5Crossref (53) 54Wilson Diseberg Gordon Zivkovic Tatarczuch Mackie al.Comprehensive cartilage formation maturation sequential extraction label-free quantitative proteomics.Mol. 1296-1313Abstract (63) 55Belluoccio Wilson Thornton D.J. Wallis Gorman J.J. mouse plate cartilage.Proteomics. 6549-6553Crossref (30) 56Hansen Kiemele O'Brien Shankar Fornetti al.An in-solution ultrasonication-assisted digestion improved proteome coverage.Mol. 8: 1648-1657Abstract (85) Of note, sample preparation protocols tailored account posed (insolubility, glycosylation), separation 1D resulted identification nearly 100 distinct (57Didangelos Yin X. Mandal Baumert Jahangiri Mayr Proteomics space components human aorta.Mol. 2048-2062Abstract (214) 58Didangelos Saje Smith Xu Q. abdominal aortic aneurysms: approach.Mol. 10https://doi.org/10.1074/mcp.M111.008128Abstract (146) However, most studies, known proteins, expected detected those were identified. One then ask: ensure capturing tissues? And indeed, faced when attempting characterize, unbiased manner, lack defined parts systematically annotate experimental output. result, days proteomics, listed "ECM" involved adhesions incorporated Conversely, prior knowledge existed would fail annotated belonging represented any attempt aiming states. became obvious analytical decipher discuss enhancement purpose biomarker target focus Special Issue Clinical Proteomics, article highlight selected performed samples rodent models show promise. organisms, zebrafish (59Chen W.C.W. Wang Missinato Park Long Liu H.J. al.Decellularized cardiac mammalian heart regeneration.Sci. Adv. 2e1600844Crossref (83) 60Garcia-Puig Mosquera Jiménez-Delgado García-Pastor Jorba Navajas al.Proteomics regeneration.Mol. 1745-1755Abstract 61Kessels M.Y. Huitema L.F.A. Boeren Kranenbarg Schulte-Merker Leeuwen JL skeletal matrix.PLoS One. 9e90568Crossref (32) drosophila (62Sessions A.O. Kaushik Parker Raedschelders Bodmer Eyk downregulation Drosophila preserves contractile function improves lifespan.Matrix 2017; 62: 15-27Crossref (15) planarians (63Sonpho E. Mann F.G. Levy Ross Guerrero-Hernández Florens al.Decellularization Enables planarian 20100137Abstract produced culture. advance fundamental disease. bottom-up MS-based but, worth noting modalities facets glycosylation patterns glycomics (64Raghunathan Sethi M.K. Klein Zaia glycomics, glycoproteomics molecules.Mol. 2138-2148Abstract (29) 65de Haan Pučić-Baković Novokmet Falck Lageveen-Kammeijer Razdorov al.Developments perspectives high-throughput glycomics: enabling thousands samples.Glycobiology. 32: 651-663Crossref 66Kellman B.P. Lewis N.E. Big-data tools connect glycan biosynthesis communication.Trends 46: 284-300Abstract (23) 67Riley N.M. Bertozzi C.R. Pitteri S.J. A pragmatic spectrometry-based glycoproteomics.Mol. 20100029Abstract fragments degradomics (68Haack Overall C.M. auf dem Keller Degradomics technologies exploration.Matrix 114: 1-17Crossref localization distribution MS (69Angel P.M. Comte-Walters Ball L.E. Talbot Brockbank K.G.M. al.Mapping formalin-fixed, paraffin-embedded MALDI spectrometry.J. Proteome 635-646Crossref (51) 70Clift C.L. Drake R.R. Angel Multiplexed serial enzyme digests formalin-fixed sections.Anal. Bioanal. Chem. 413: 2709-2719Crossref (8) 2012, published journal describing two-pronged (71Naba Clauser K.R. Hoersch Carr Hynes matrisome: silico definition normal matrices.Mol. 11https://doi.org/10.1074/mcp.M111.014647Abstract (668) While had attempted limitations described above decellularizing extracting guanidine hydrochloride), set tackle them all. brief, took differential solubility deplete non-ECM incubations extraction, decellularization, buffers concomitantly enriching Observing incubation 8 M urea mM DTT did fully solubilize ECM-enriched suspecting found material, processed "crude" M-urea-resuspended samples. We hypothesized deglycosylating enhance accessibility treated Peptide-N-glycosidase F (PNGaseF). further preincubated deglycosylated suspension LysC, protease digesting tightly folded tryptic digestion. To fractionated off-gel electrophoresis. Last, quantification stipulated ECM-specific PTMs hydroxylations variable database search. Indeed, 19% acid sequence I positions X Y X-Y-Gly repeats often hydroxylated parallel, developed robust nomenclature classify characteristic domain-based organization (72Hohenester Eng

Language: Английский

Citations

56
Design and engineering of organ-on-a-chip DOI Open Access
Su Jin Cho, Sumi Lee, Song Ih Ahn

et al.

Biomedical Engineering Letters, Journal Year: 2023, Volume and Issue: 13(2), P. 97 - 109

Published: Jan. 2, 2023

Language: Английский

Citations

44
CAF-induced physical constraints controlling T cell state and localization in solid tumours DOI
Ludovica Arpinati,

Giulia Carradori,

Ruth Scherz‐Shouval

et al.

Nature reviews. Cancer, Journal Year: 2024, Volume and Issue: 24(10), P. 676 - 693

Published: Sept. 9, 2024

Language: Английский

Citations

20
Advancement of Scaffold-Based 3D Cellular Models in Cancer Tissue Engineering: An Update DOI Creative Commons

Kavitha Unnikrishnan,

Lynda V. Thomas, Ram Mohan Ram Kumar

et al.

Frontiers in Oncology, Journal Year: 2021, Volume and Issue: 11

Published: Oct. 25, 2021

The lack of traditional cancer treatments has resulted in an increased need for new clinical techniques. Standard two-dimensional (2D) models used to validate drug efficacy and screening have a low

Language: Английский

Citations

69