Biofilm microenvironment triggered self-enhancing photodynamic immunomodulatory microneedle for diabetic wound therapy

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Ноя. 23, 2023

The treatment of diabetic wounds faces enormous challenges due to complex wound environments, such as infected biofilms, excessive inflammation, and impaired angiogenesis. critical role the microenvironment in chronic has not been addressed for therapeutic development. Herein, we develop a microneedle (MN) bandage functionalized with dopamine-coated hybrid nanoparticles containing selenium chlorin e6 (SeC@PA), which is capable dual-directional regulation reactive species (RS) generation, including oxygen (ROS) nitrogen (RNS), response microenvironment. SeC@PA MN can disrupt barriers coverings efficient delivery. only depletes endogenous glutathione (GSH) enhance anti-biofilm effect RS, but also degrades GSH biofilms through cascade reactions generate more lethal RS biofilm eradication. acts an scavenger beds low levels, exerting anti-inflammatory effect. promotes M2-phenotype polarization macrophages, accelerating healing. This self-enhanced, catabolic dynamic therapy, activated by microenvironment, provides approach treating wounds.

Язык: Английский

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A Nanozyme‐Immobilized Hydrogel with Endogenous ROS‐Scavenging and Oxygen Generation Abilities for Significantly Promoting Oxidative Diabetic Wound Healing

Advanced Healthcare Materials, Год журнала: 2022, Номер 11(22)

Опубликована: Сен. 14, 2022

Abstract Non‐healing wound is a common complication of diabetic patients associated with high morbidity and mortality. Engineered therapeutic hydrogels have enviable advantages in tissue regeneration, however, they are suboptimal for the healing wounds characterized by reactive oxygen species (ROS) accumulation chronic hypoxia. Here, unique biological metabolism‐inspired hydrogel, ameliorating this hostile microenvironment, presented. Consisting natural polymers (hydrazide modified hyaluronic acid aldehyde acid) metal–organic frameworks derived catalase‐mimic nanozyme ( ε ‐polylysine coated mesoporous manganese cobalt oxide), engineered nanozyme‐reinforced can not only capture endogenous elevated ROS wounds, but also synergistically produce through ROS‐driven production ability. These fascinating properties protect skin cells (e.g., keratinocytes, fibroblasts, vascular endothelial cells) from hypoxia‐mediated death proliferation inhibition. Diabetic treated highlight potential inducing macrophages polarization pro‐inflammatory phenotype (M1) to anti‐inflammatory subtype (M2). The hydrogel dressings demonstrate prominently accelerated rate as shown alleviating excessive inflammatory, efficiently proliferation, re‐epithelialization, collagen deposition, neovascularization. This work provides an effective strategy based on oxygenerator enhancing healing.

Язык: Английский

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Nanoparticle-based therapeutic approaches for wound healing: a review of the state-of-the-art

Materials Today Chemistry, Год журнала: 2022, Номер 27, С. 101319 - 101319

Опубликована: Дек. 21, 2022

Язык: Английский

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Developing natural polymers for skin wound healing

Bioactive Materials, Год журнала: 2023, Номер 33, С. 355 - 376

Опубликована: Ноя. 26, 2023

Natural polymers are complex organic molecules that occur in the natural environment and have not been subjected to artificial synthesis. They frequently encountered various creatures, including mammals, plants, microbes. The aforementioned commonly derived from renewable sources, possess a notable level of compatibility with living organisms, limited adverse effect on environment. As result, they hold considerable significance development sustainable environmentally friendly goods. In recent times, there has advancement investigation potential uses field biomedicine, specifically relation biomaterials exhibit antibacterial antioxidant characteristics. This review provides comprehensive overview prevalent utilized biomedical domain throughout preceding two decades. this paper, we present examination components typical methods for preparation based polymers. Furthermore, summarize application polymer materials each stage skin wound repair. Finally, key findings insights into limitations current elucidate prospects their future field.

Язык: Английский

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In Situ 3D Bioprinting Living Photosynthetic Scaffolds for Autotrophic Wound Healing

Research, Год журнала: 2022, Номер 2022

Опубликована: Янв. 1, 2022

Three-dimensional (3D) bioprinting has been extensively explored for tissue repair and regeneration, while the insufficient nutrient oxygen availability in printed constructs, as well lack of adaptive dimensions shapes, compromises overall therapeutic efficacy limits their further application. Herein, inspired by natural symbiotic relationship between salamanders algae, we present novel living photosynthetic scaffolds using an situ microfluidic-assisted 3D strategy adapting irregular-shaped wounds promoting healing. As oxygenic photosynthesis unicellular microalga (Chlorella pyrenoidosa) was incorporated during printing, generated could produce sustainable under light illumination, which facilitated cell proliferation, migration, differentiation even hypoxic conditions. Thus, when microalgae-laden were directly into diabetic wounds, they significantly accelerate chronic wound closure alleviating local hypoxia, increasing angiogenesis, extracellular matrix (ECM) synthesis. These results indicate that microalgae offers effective autotrophic biosystem healing, suggesting a promising diverse engineering applications.

Язык: Английский

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A composite hydrogel containing resveratrol-laden nanoparticles and platelet-derived extracellular vesicles promotes wound healing in diabetic mice

Acta Biomaterialia, Год журнала: 2022, Номер 154, С. 212 - 230

Опубликована: Окт. 27, 2022

Язык: Английский

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Bioinspired Polyacrylic Acid‐Based Dressing: Wet Adhesive, Self‐Healing, and Multi‐Biofunctional Coacervate Hydrogel Accelerates Wound Healing

Advanced Science, Год журнала: 2023, Номер 10(16)

Опубликована: Апрель 14, 2023

Polyacrylic acid (PAA) and its derivatives are commonly used as essential matrices in wound dressings, but their weak wet adhesion restricts the clinical application. To address this issue, a PAA-based coacervate hydrogel with strong capability is fabricated through facile mixture of PAA copolymers isoprenyl oxy poly(ethylene glycol) ether tannic (TA). The segments on prevent electrostatic repulsion among ionized carboxyl groups absorbed TA to form coacervates. provides solid dry substrates via multifarious interactions, which endows an adhesive strength skin 23.4 kPa 70% underwater. This achieves desirable self-healing extensible properties suitable for frequently moving joints. These investigations prove that has antibacterial activity, facilitates fibroblast migration, modulates M1/M2 polarization macrophages. In vivo hemorrhage experiments further confirm dramatically shortens hemostatic time from hundreds tens seconds. addition, full-thickness defect demonstrate best therapeutic effect by significantly promoting collagen deposition, angiogenesis, epithelialization. results promising dressing medical translation.

Язык: Английский

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Reactive Oxygen Species‐Scavenging Nanosystems in the Treatment of Diabetic Wounds

Advanced Healthcare Materials, Год журнала: 2023, Номер 12(25)

Опубликована: Апрель 13, 2023

Diabetic wounds are characterized by drug-resistant bacterial infections, biofilm formation, impaired angiogenesis and perfusion, oxidative damage to the microenvironment. Given their complex nature, diabetic remain a major challenge in clinical practice. Reactive oxygen species (ROS), which have been shown trigger hyperinflammation excessive cellular apoptosis, play pivotal role pathogenesis of wounds. ROS-scavenging nanosystems recently emerged as smart multifunctional nanomedicines with broad synergistic applicability. The documented anti-inflammatory pro-angiogenic ability treatments predestines these promising options for treatment Yet, this context, therapeutic applicability efficacy be elucidated. Herein, ROS is deciphered, properties strengths capacity summarized. In addition, current challenges such potential future directions discussed through clinical-translational lens.

Язык: Английский

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Multifunctional Silk Fibroin Methacryloyl Microneedle for Diabetic Wound Healing

Small, Год журнала: 2022, Номер 18(51)

Опубликована: Ноя. 4, 2022

Diabetic wound is one of the common complications in diabetic patients, which exhibits chronic, hard-to-heal characteristics. The healing process wounds impaired by several factors, including excessive oxidative stress, blocked angiogenesis, and bacterial infection. therapeutic effects traditional microneedle patches remain not satisfactory, due to their difficulty simultaneously targeting multiple targets treat wounds. As such, there an urgent need develop a multifunctional (MN) patch for promoting A MN with antioxidant, proangiogenesis, antibacterial capacities was fabricated target pathogenesis Silk fibroin methacryloyl, has excellent biocompatibility, stable mechanical properties, well processability, selected as base material patches. Prussian blue nanozymes (PBNs) vascular endothelial growth factor (VEGF) are encapsulated tips patches, Polymyxin layers Based on synergic properties these components, exhibit drug-sustained release, properties. developed accelerate healing, providing potential approach.

Язык: Английский

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Diabetes immunity-modulated multifunctional hydrogel with cascade enzyme catalytic activity for bacterial wound treatment

Biomaterials, Год журнала: 2022, Номер 289, С. 121790 - 121790

Опубликована: Сен. 5, 2022

Язык: Английский

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