Synaptic connectome of the Drosophila circadian clock

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Дек. 5, 2024

Abstract The circadian clock and its output pathways play a pivotal role in optimizing daily processes. To obtain insights into how diverse rhythmic physiology behaviors are orchestrated, we have generated comprehensive connectivity map of an animal using the Drosophila FlyWire brain connectome. Intriguingly, identified additional dorsal neurons, thus showing that network contains ~240 instead 150 neurons. We revealed extensive contralateral synaptic within discovered novel indirect light input to also elucidated via which modulates descending neurons known regulate feeding reproductive behaviors. Interestingly, observed sparse monosynaptic between downstream higher-order centers neurosecretory cells behavior physiology. Therefore, integrated single-cell transcriptomics receptor mapping decipher putative paracrine peptidergic signaling by Our analyses neuropeptides expressed suggest significantly enriches interconnectivity network.

Язык: Английский

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Mutual coupling of neurons in the circadian master clock: what we can learn from fruit flies

Neurobiology of Sleep and Circadian Rhythms, Год журнала: 2025, Номер 18, С. 100112 - 100112

Опубликована: Янв. 18, 2025

Circadian master clocks in the brain consist of multiple neurons that are organized into populations with different morphology, physiology, and neuromessenger content presumably functions. In most animals, these distributed bilaterally, located close proximity to visual system, synchronized by eyes light-dark cycles environment. mammals cockroaches, each two consists a core region receives information from shell which output projections originate, whereas flies several other insects, lateral dorsal regions. all cases, morning evening clock seem exist, communication between them neurons, as well connection across hemispheres, is prerequisite for normal rhythmic function. Phenomena such rhythm splitting, internal desynchronization caused "decoupling" hemispheres or decoupling certain within one hemisphere. Since contain relatively few characterized at individual level, fly particularly suited study neurons. Here, we review organization bilateral brain, focus on synaptic paracrine connections comparison insects mammals.

Язык: Английский

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Synaptic connectome of theDrosophilacircadian clock

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Сен. 13, 2023

The circadian clock and its output pathways play a pivotal role in optimizing daily processes. To obtain novel insights into how diverse rhythmic physiology behaviors are orchestrated, we have generated the first comprehensive connectivity map of an animal using Drosophila FlyWire brain connectome. Intriguingly, identified additional dorsal neurons, thus showing that network contains ∼240 instead 150 neurons. We also revealed extensive contralateral synaptic within discovered indirect light input to Interestingly, observed sparse monosynaptic between neurons down-stream higher-order centers neurosecretory cells known regulate behavior physiology. Therefore, integrated single-cell transcriptomics receptor mapping decipher putative paracrine peptidergic signaling by Our analyses neuropeptides expressed suggest significantly enriches interconnectivity network.

Язык: Английский

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A single photoreceptor splits perception and entrainment by cotransmission

Nature, Год журнала: 2023, Номер 623(7987), С. 562 - 570

Опубликована: Окт. 25, 2023

Vision enables both image-forming perception, driven by a contrast-based pathway, and unconscious non-image-forming circadian photoentrainment, an irradiance-based pathway1,2. Although two distinct photoreceptor populations are specialized for each visual task3-6, photoreceptors can additionally contribute to photoentrainment of the clock in different species7-15. However, it is unknown how pathway functionally implement segregation irradiance signals required from contrast image perception. Here we report that Drosophila R8 separates co-transmitting neurotransmitters, histamine acetylcholine. This further established postsynaptically histamine-receptor-expressing unicolumnar retinotopic neurons acetylcholine-receptor-expressing multicolumnar integration neurons. The acetylcholine transmission sustained autocrine negative feedback cotransmitted during light phase light-dark cycles. At behavioural level, elimination impairs R8-driven motion detection respectively. Thus, single type achieve dichotomy perception as early first synapses, revealing simple yet robust mechanism segregate translate sensory features into animal behaviours.

Язык: Английский

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A subclass of evening cells promotes the switch from arousal to sleep at dusk

Current Biology, Год журнала: 2024, Номер 34(10), С. 2186 - 2199.e3

Опубликована: Май 1, 2024

Язык: Английский

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Light-induced negative differential resistance and neural oscillations in neuromorphic photonic semiconductor micropillar sensory neurons

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 25, 2025

Neuromorphic systems, inspired by nature, are sought to efficiently process analogue inputs in real and complex environments. This could lead ultralow-power in-sensor intelligent edge computers. Here, we present an artificial sensory oscillator neuron consisting of a III–V semiconductor micropillar quantum resonant tunnelling diode (RTD) with GaAs photosensitive absorption layers. The oscillatory optical encodes incoming data into spatiotemporal signals. We demonstrate that near-infrared light within certain intensity range activates region negative differential resistance, subsequently, large-amplitude voltage oscillations. As result, optic information is encoded electrical oscillations resulting amplification inputs. Under pulse-modulated light, excitation inhibition burst firing patterns can be controlled single neuron, simulating neural activity networks the form breather-type phenomena. Such (burst firing) basis for combined sensing, pre-processing, encoding abilities vision-nervous system found biological organisms. work paves way future visual systems using nano-optoelectronic circuits applications light-driven neurorobotics, bioinspired optoelectronics, neuromorphic computing real-time processing data.

Язык: Английский

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Regulation of pre-dawn arousal in Drosophila by a pair of trissinergic descending neurons of the visual and circadian networks

Current Biology, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

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Contribution of membrane-associated oscillators to biological timing at different timescales

Frontiers in Physiology, Год журнала: 2024, Номер 14

Опубликована: Янв. 9, 2024

Environmental rhythms such as the daily light-dark cycle selected for endogenous clocks. These clocks predict regular environmental changes and provide basis well-timed adaptive homeostasis in physiology behavior of organisms. Endogenous are oscillators that based on positive feedforward negative feedback loops. They generate stable even under constant conditions. Since weak interactions between allow autonomous synchronization, coupling/synchronization provides self-organized physiological timing. Amongst most thoroughly researched circadian clock neurons mammals insects. comprise nuclear clockworks transcriptional/translational loops (TTFL) ∼24 h gene expression entrained to day-night cycle. It is generally assumed this TTFL clockwork drives all oscillations within cells, being any rhythm Instead current gene-based hierarchical model we here a systems view We suggest coupled system posttranslational loop (PTFL) oscillators/clocks run at multiple timescales governs adaptive, dynamic behavior. focus mammalian insect timescales. neuronal plasma membrane-associated signalosomes constitute specific PTFL localized but interlinked membrane potential intracellular messengers with frequencies. In each neuron multiscale form temporally structured oscillatory network common complex frequency-band comprising superimposed oscillations. Coupling oscillator/clock next level complexity an network. This systemic molecular cellular suggested cycles through homeostatic setpoints characteristic hallmark. propose mechanisms plasticity maintain stability these setpoints, whereas Hebbian enables switching via coupling factors, like biogenic amines and/or neuropeptides. reprogram new frequency, setpoint. Our novel hypothesis up experimental challenge.

Язык: Английский

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Nanoarchitectonics of Vesicle Microreactors for Oscillating ATP Synthesis and Hydrolysis

Angewandte Chemie International Edition, Год журнала: 2024, Номер 63(45)

Опубликована: Июль 23, 2024

Abstract We construct a compartmentalized nanoarchitecture to regulate bioenergy level. Glucose dehydrogenase, urease and nicotinamide adenine dinucleotide are encapsulated inside through liquid‐liquid phase separation. ATPase glucose transporter embedded in hybrid liposomes attached at the surface. is transported converted gluconic acid catalyzed by resulting an outward proton gradient drive for ATP synthesis. In parallel, catalyzes hydrolysis of urea generate ammonia, which leads inward hydrolysis. These processes lead change direction gradient, thus achieving artificial oscillation. Importantly, frequency amplitude oscillation can be programmed. The work explores nanoarchitectonics integrating multiple components realize precise

Язык: Английский

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Nanoarchitectonics of Vesicle Microreactors for Oscillating ATP Synthesis and Hydrolysis

Angewandte Chemie, Год журнала: 2024, Номер 136(45)

Опубликована: Июль 23, 2024

Abstract We construct a compartmentalized nanoarchitecture to regulate bioenergy level. Glucose dehydrogenase, urease and nicotinamide adenine dinucleotide are encapsulated inside through liquid‐liquid phase separation. ATPase glucose transporter embedded in hybrid liposomes attached at the surface. is transported converted gluconic acid catalyzed by resulting an outward proton gradient drive for ATP synthesis. In parallel, catalyzes hydrolysis of urea generate ammonia, which leads inward hydrolysis. These processes lead change direction gradient, thus achieving artificial oscillation. Importantly, frequency amplitude oscillation can be programmed. The work explores nanoarchitectonics integrating multiple components realize precise

Язык: Английский

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