Buoyant Metal–Organic Framework Corona-Driven Fast Isolation and Ultrasensitive Profiling of Circulating Extracellular Vesicles

ACS Nano, Год журнала: 2024, Номер 18(22), С. 14569 - 14582

Опубликована: Май 23, 2024

Accurately assaying tumor-derived circulating extracellular vesicles (EVs) is fundamental in noninvasive cancer diagnosis and therapeutic monitoring but limited by challenges efficient EV isolation profiling. Here, we report a bioinspired buoyancy-driven metal-organic framework (MOF) corona that leverages on-bubble coordination dual-encoded surface-enhanced Raman scattering (SERS) nanotags to streamline rapid ultrasensitive profiling of plasma EVs single assay for diagnostics. This integrated bubble-MOF-SERS (IBMsv) allows barnacle-like high-density adhesion MOFs on self-floating bubble surface enable fast (2 min, near 90% capture efficiency) tumor via enhanced EV-MOF binding. Also, IBMsv harnesses four-plexed SERS profile the captured protein markers at single-particle level. Such sensitive multiplexed across five types, revealing heterogeneous expression patterns. Furthermore, enables pilot clinical cohort (

Язык: Английский

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Exosomal Liquid Biopsy in Prostate Cancer: A Systematic Review of Biomarkers for Diagnosis, Prognosis, and Treatment Response

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(2), С. 802 - 802

Опубликована: Янв. 18, 2025

Prostate cancer, a leading cause of cancer-related mortality among men, often presents challenges in accurate diagnosis and effective monitoring. This systematic review explores the potential exosomal biomolecules as noninvasive biomarkers for diagnosis, prognosis, treatment response prostate cancer. A thorough literature search through online public databases (Medline via PubMed, Scopus, Web science) using structured terms screening predefined eligibility criteria resulted 137 studies that we analyzed this review. We evaluated findings from these clinical studies, revealing load exosomes blood urine cancer patients, which includes microRNAs (miRNAs), proteins, lipids, demonstrates disease-specific changes. It also shows some markers can differentiate between malignant benign hyperplasia prostate, predict disease aggressiveness, monitor efficacy. Notably, miRNA emerged most frequently studied biomolecule, demonstrating superior diagnostic compared to traditional methods like prostate-specific antigen (PSA) testing. The analysis highlights pressing need standardised analytic approach multi-centre validate full monitoring

Язык: Английский

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Schwann Cell-Derived Exosomes and Methylprednisolone Composite Patch for Spinal Cord Injury Repair

ACS Nano, Год журнала: 2023, Номер 17(22), С. 22928 - 22943

Опубликована: Ноя. 10, 2023

Spinal cord injury (SCI) can cause permanent loss of sensory and motor function, there is no effective clinical treatment, to date. Due the complex pathological process involved after injury, synergistic treatments are very urgently needed in practice. We designed a nanofiber scaffold hyaluronic acid hydrogel patch release both exosomes methylprednisolone injured spinal non-invasive manner. This composite showed good biocompatibility stabilization exosome morphology toxicity nerve cells. Meanwhile, increased proportion M2-type macrophages reduced neuronal apoptosis an vitro study. In vivo, functional electrophysiological performance rats with SCI was significantly improved when covered surface hematoma. The inhibited inflammatory response through macrophage polarization from M1 type M2 survival neurons by inhibition SCI. therapeutic effects this be attributed TLR4/NF-κB, MAPK, Akt/mTOR pathways. Thus, provides medicine-exosomes dual-release system may provide method for treatment individuals

Язык: Английский

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Advances of machine learning-assisted small extracellular vesicles detection strategy

Biosensors and Bioelectronics, Год журнала: 2024, Номер 251, С. 116076 - 116076

Опубликована: Фев. 1, 2024

Язык: Английский

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Renewable Electrochemiluminescence Biosensor Based on Eu-MOGs as a Highly Efficient Emitter and a DNAzyme-Mediated Dual-drive DNA Walker as a Signal Amplifier for Ultrasensitive Detection of miRNA-222

Analytical Chemistry, Год журнала: 2024, Номер 96(11), С. 4589 - 4596

Опубликована: Март 5, 2024

Herein, novel europium metal–organic gels (Eu-MOGs) with excellent cathode electrochemiluminescence (ECL) emission are first used to construct biosensors for the ultrasensitive detection of miRNA-222. Impressively, N and O elements organic ligand 2,2′:6,2″-terpyridine 4,4′,4″-tricarboxylic acid (H3-tctpy) can perfectly coordinate Eu3+ form Eu-MOGs, which not only reduce nonradiative transition caused by intramolecular free rotation phenyl rings in other MOGs enhance ECL signal extraordinary efficiency as high 37.2% (vs [Ru(bpy)3]2+/S2O82– system) but also reinforce ligand-to-metal charge transfer (LMCT) strong affinity between greatly improve stability signals. Besides, an improved nucleic cascade amplification reaction is developed raise conversion from target miRNA-222 a DNAzyme-mediated dual-drive DNA walker output DNA, simultaneously shear specific recognition sites two directions. In that way, proposed biosensor further sensitivity linear range 10 aM–1 nM limit (LOD) 8.5 aM, achieve accurate response cancer cell lysates MHCC-97L HeLa. Additionally, be self-regenerated folding/unfolding related triplets pH changes simplify experimental operations cost. Hence, this work stable intense signals construction renewable biosensor, supplying reliable method biomarker analysis disease diagnosis.

Язык: Английский

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Ultrahigh-Speed 3D DNA Walker with Dual Self-Protected DNAzymes for Ultrasensitive Fluorescence Detection and Intracellular Imaging of microRNA

Analytical Chemistry, Год журнала: 2024, Номер 96(24), С. 9866 - 9875

Опубликована: Июнь 5, 2024

Herein, a dual self-protected DNAzyme-based 3D DNA walker (dSPD walker), composed of activated walking particles (ac-dSPWPs) and track (TPs), was constructed for ultrasensitive ultrahigh-speed fluorescence detection imaging microRNAs (miRNAs) in living cells. Impressively, compared with the defect that "one" target miRNA only initiates arm conventional single DNAzyme walker, dSPD benefits from secondary amplification spatial confinement effect could guide to generate "n" targets, thereby initiating nearby strands immediately, realizing initial rate over one-magnitude-order faster than one. Moreover, process relative motion between ac-dSPWPs TPs, cleave multiple substrate simultaneously speed up movement reduce derailment rate, as well combine successive TPs facilitate large amount continuous signal accumulation, achieving an ultrafast miRNA-221 within 10 min vitro high sensitivity low limit 0.84 pM. In addition, nanospheres obtained by rolling circle reaction can capture Cy5 dispersed liquids, which achieves high-contrast miRNA-221, resulting further cancer The proposed strategy has made bold innovation rapid sensitive intracellular low-abundance biomarkers, offering promising application early diagnosis relevant research tumors.

Язык: Английский

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CRISPR-Enhanced Photocurrent Polarity Switching for Dual-lncRNA Detection Combining Deep Learning for Cancer Diagnosis

Analytical Chemistry, Год журнала: 2024, Номер 96(32), С. 13278 - 13284

Опубликована: Авг. 2, 2024

Abnormal expression in long noncoding RNAs (lncRNAs) is closely associated with cancers. Herein, a novel CRISPR/Cas13a-enhanced photocurrent-polarity-switching photoelectrochemical (PEC) biosensor was engineered for the joint detection of dual lncRNAs, using deep learning (DL) to assist cancer diagnosis. After target lncRNA-activated CRISPR/Cas13a cleaves induce DNAzyme bidirectional walkers help cofactor Mg

Язык: Английский

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The new advance of exosome-based liquid biopsy for cancer diagnosis

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Окт. 8, 2024

Liquid biopsy is a minimally invasive method that uses biofluid samples instead of tissue for cancer diagnosis. Exosomes are small extracellular vesicles secreted by donor cells and act as mediators intercellular communication in human health disease. Due to their important roles, exosomes have been considered promising biomarkers liquid biopsy. However, traditional methods exosome isolation cargo detection time-consuming inefficient, limiting practical application. In the past decades, many new strategies, such microfluidic chips, nanowire arrays electrochemical biosensors, proposed achieve rapid, accurate high-throughput analysis exosomes. this review, we discussed about advance exosome-based technology, including isolation, enrichment, approaches. The comparison currently available also included. Finally, summarized advantages limitations present strategies further gave perspective future translational use.

Язык: Английский

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Recent Advances in Aptamer-Based Microfluidic Biosensors for the Isolation, Signal Amplification and Detection of Exosomes

Sensors, Год журнала: 2025, Номер 25(3), С. 848 - 848

Опубликована: Янв. 30, 2025

Exosomes carry diverse tumor-associated molecular information that can reflect real-time tumor progression, making them a promising tool for liquid biopsy. However, traditional methods exosome isolation and detection often rely on large, expensive equipment are time-consuming, limiting their practical applicability in clinical settings. Microfluidic technology offers versatile platform analysis, with advantages such as seamless integration, portability reduced sample volumes. Aptamers, which single-stranded oligonucleotides high affinity specificity target molecules, have been frequently employed the development of aptamer-based microfluidics isolation, signal amplification, quantitative exosomes. This review summarizes recent advances microfluidic strategies including (1) on-chip capture mediated by aptamers combined nanomaterials or nanointerfaces; (2) amplification techniques, enzyme-free hybridization chain reaction (HCR), rolling circle (RCA), DNA machine-assisted amplification; (3) various aptamer-assisted methods, fluorescence, electrochemistry, surface-enhanced Raman scattering (SERS), magnetism. The limitations these also summarized. Finally, future challenges directions analysis exosomes based discussed.

Язык: Английский

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Machine Learning-Aided Identification of Fecal Extracellular Vesicle microRNA Signatures for Noninvasive Detection of Colorectal Cancer

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

Colorectal cancer (CRC) remains a formidable threat to human health, with considerable challenges persisting in its diagnosis, particularly during the early stages of malignancy. In this study, we elucidated that fecal extracellular vesicle microRNA signatures (FEVOR) could serve as potent noninvasive CRC biomarkers. FEVOR was first revealed by miRNA sequencing, followed construction CRISPR/Cas13a-based detection platform interrogate expression across diverse spectrum clinical cohorts. Machine learning-driven models were subsequently developed within realms diagnostics, prognostics, and warning systems. cohort 38 patients, our diagnostic model achieved an outstanding accuracy 97.4% (37/38), successfully identifying 37 cases. This performance significantly outpaced efficacy two clinically established biomarkers, CEA CA19-9, which showed accuracies mere 26.3% (10/38) 7.9% (3/38), respectively. We also examined levels several patients both before after surgery, well colorectal adenomas (CA). Impressively, results robust prognostic indicator for potential predictor CA. endeavor aimed harness predictive power enhancing precision management paradigms. envision these findings will propel foundational preclinical research on CRC, studies.

Язык: Английский

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