ACS Nano,
Год журнала:
2024,
Номер
18(22), С. 14569 - 14582
Опубликована: Май 23, 2024
Accurately
assaying
tumor-derived
circulating
extracellular
vesicles
(EVs)
is
fundamental
in
noninvasive
cancer
diagnosis
and
therapeutic
monitoring
but
limited
by
challenges
efficient
EV
isolation
profiling.
Here,
we
report
a
bioinspired
buoyancy-driven
metal-organic
framework
(MOF)
corona
that
leverages
on-bubble
coordination
dual-encoded
surface-enhanced
Raman
scattering
(SERS)
nanotags
to
streamline
rapid
ultrasensitive
profiling
of
plasma
EVs
single
assay
for
diagnostics.
This
integrated
bubble-MOF-SERS
(IBMsv)
allows
barnacle-like
high-density
adhesion
MOFs
on
self-floating
bubble
surface
enable
fast
(2
min,
near
90%
capture
efficiency)
tumor
via
enhanced
EV-MOF
binding.
Also,
IBMsv
harnesses
four-plexed
SERS
profile
the
captured
protein
markers
at
single-particle
level.
Such
sensitive
multiplexed
across
five
types,
revealing
heterogeneous
expression
patterns.
Furthermore,
enables
pilot
clinical
cohort
(
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(2), С. 802 - 802
Опубликована: Янв. 18, 2025
Prostate
cancer,
a
leading
cause
of
cancer-related
mortality
among
men,
often
presents
challenges
in
accurate
diagnosis
and
effective
monitoring.
This
systematic
review
explores
the
potential
exosomal
biomolecules
as
noninvasive
biomarkers
for
diagnosis,
prognosis,
treatment
response
prostate
cancer.
A
thorough
literature
search
through
online
public
databases
(Medline
via
PubMed,
Scopus,
Web
science)
using
structured
terms
screening
predefined
eligibility
criteria
resulted
137
studies
that
we
analyzed
this
review.
We
evaluated
findings
from
these
clinical
studies,
revealing
load
exosomes
blood
urine
cancer
patients,
which
includes
microRNAs
(miRNAs),
proteins,
lipids,
demonstrates
disease-specific
changes.
It
also
shows
some
markers
can
differentiate
between
malignant
benign
hyperplasia
prostate,
predict
disease
aggressiveness,
monitor
efficacy.
Notably,
miRNA
emerged
most
frequently
studied
biomolecule,
demonstrating
superior
diagnostic
compared
to
traditional
methods
like
prostate-specific
antigen
(PSA)
testing.
The
analysis
highlights
pressing
need
standardised
analytic
approach
multi-centre
validate
full
monitoring
ACS Nano,
Год журнала:
2023,
Номер
17(22), С. 22928 - 22943
Опубликована: Ноя. 10, 2023
Spinal
cord
injury
(SCI)
can
cause
permanent
loss
of
sensory
and
motor
function,
there
is
no
effective
clinical
treatment,
to
date.
Due
the
complex
pathological
process
involved
after
injury,
synergistic
treatments
are
very
urgently
needed
in
practice.
We
designed
a
nanofiber
scaffold
hyaluronic
acid
hydrogel
patch
release
both
exosomes
methylprednisolone
injured
spinal
non-invasive
manner.
This
composite
showed
good
biocompatibility
stabilization
exosome
morphology
toxicity
nerve
cells.
Meanwhile,
increased
proportion
M2-type
macrophages
reduced
neuronal
apoptosis
an
vitro
study.
In
vivo,
functional
electrophysiological
performance
rats
with
SCI
was
significantly
improved
when
covered
surface
hematoma.
The
inhibited
inflammatory
response
through
macrophage
polarization
from
M1
type
M2
survival
neurons
by
inhibition
SCI.
therapeutic
effects
this
be
attributed
TLR4/NF-κB,
MAPK,
Akt/mTOR
pathways.
Thus,
provides
medicine-exosomes
dual-release
system
may
provide
method
for
treatment
individuals
Analytical Chemistry,
Год журнала:
2024,
Номер
96(11), С. 4589 - 4596
Опубликована: Март 5, 2024
Herein,
novel
europium
metal–organic
gels
(Eu-MOGs)
with
excellent
cathode
electrochemiluminescence
(ECL)
emission
are
first
used
to
construct
biosensors
for
the
ultrasensitive
detection
of
miRNA-222.
Impressively,
N
and
O
elements
organic
ligand
2,2′:6,2″-terpyridine
4,4′,4″-tricarboxylic
acid
(H3-tctpy)
can
perfectly
coordinate
Eu3+
form
Eu-MOGs,
which
not
only
reduce
nonradiative
transition
caused
by
intramolecular
free
rotation
phenyl
rings
in
other
MOGs
enhance
ECL
signal
extraordinary
efficiency
as
high
37.2%
(vs
[Ru(bpy)3]2+/S2O82–
system)
but
also
reinforce
ligand-to-metal
charge
transfer
(LMCT)
strong
affinity
between
greatly
improve
stability
signals.
Besides,
an
improved
nucleic
cascade
amplification
reaction
is
developed
raise
conversion
from
target
miRNA-222
a
DNAzyme-mediated
dual-drive
DNA
walker
output
DNA,
simultaneously
shear
specific
recognition
sites
two
directions.
In
that
way,
proposed
biosensor
further
sensitivity
linear
range
10
aM–1
nM
limit
(LOD)
8.5
aM,
achieve
accurate
response
cancer
cell
lysates
MHCC-97L
HeLa.
Additionally,
be
self-regenerated
folding/unfolding
related
triplets
pH
changes
simplify
experimental
operations
cost.
Hence,
this
work
stable
intense
signals
construction
renewable
biosensor,
supplying
reliable
method
biomarker
analysis
disease
diagnosis.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(24), С. 9866 - 9875
Опубликована: Июнь 5, 2024
Herein,
a
dual
self-protected
DNAzyme-based
3D
DNA
walker
(dSPD
walker),
composed
of
activated
walking
particles
(ac-dSPWPs)
and
track
(TPs),
was
constructed
for
ultrasensitive
ultrahigh-speed
fluorescence
detection
imaging
microRNAs
(miRNAs)
in
living
cells.
Impressively,
compared
with
the
defect
that
"one"
target
miRNA
only
initiates
arm
conventional
single
DNAzyme
walker,
dSPD
benefits
from
secondary
amplification
spatial
confinement
effect
could
guide
to
generate
"n"
targets,
thereby
initiating
nearby
strands
immediately,
realizing
initial
rate
over
one-magnitude-order
faster
than
one.
Moreover,
process
relative
motion
between
ac-dSPWPs
TPs,
cleave
multiple
substrate
simultaneously
speed
up
movement
reduce
derailment
rate,
as
well
combine
successive
TPs
facilitate
large
amount
continuous
signal
accumulation,
achieving
an
ultrafast
miRNA-221
within
10
min
vitro
high
sensitivity
low
limit
0.84
pM.
In
addition,
nanospheres
obtained
by
rolling
circle
reaction
can
capture
Cy5
dispersed
liquids,
which
achieves
high-contrast
miRNA-221,
resulting
further
cancer
The
proposed
strategy
has
made
bold
innovation
rapid
sensitive
intracellular
low-abundance
biomarkers,
offering
promising
application
early
diagnosis
relevant
research
tumors.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(32), С. 13278 - 13284
Опубликована: Авг. 2, 2024
Abnormal
expression
in
long
noncoding
RNAs
(lncRNAs)
is
closely
associated
with
cancers.
Herein,
a
novel
CRISPR/Cas13a-enhanced
photocurrent-polarity-switching
photoelectrochemical
(PEC)
biosensor
was
engineered
for
the
joint
detection
of
dual
lncRNAs,
using
deep
learning
(DL)
to
assist
cancer
diagnosis.
After
target
lncRNA-activated
CRISPR/Cas13a
cleaves
induce
DNAzyme
bidirectional
walkers
help
cofactor
Mg
Neutrophil-derived
extracellular
vesicles
(NEVs)
are
critically
involved
in
disease
progression
and
considered
potential
biomarkers.
However,
the
tedious
processes
of
NEV
separation
detection
restrain
their
use.
Herein,
we
presented
an
integrated
microfluidic
chip
for
(IMCN)
analysis,
which
achieved
immune-separation
CD66b+
NEVs
multiplexed
contained
miRNAs
(termed
signatures)
by
using
10
μL
serum
samples.
The
optimized
microchannel
flow
rate
IMCN
enabled
efficient
capture
(>90%).
After
recognition
captured
a
specific
CD63
aptamer,
on-chip
rolling
circle
amplification
(RCA)
reaction
was
triggered
released
aptamers
from
heat-lysed
NEVs.
Then,
RCA
products
bound
to
molecular
beacons
(MBs),
initiating
allosteric
hairpin
structures
amplified
"turn
on"
fluorescence
signals
(RCA-MB
assay).
Clinical
sample
analysis
showed
that
signatures
had
high
area
under
curve
(AUC)
distinguishing
between
healthy
control
(HC)
gastric
cancer
(GC)
(0.891),
benign
diseases
(BGD)
GC
(0.857).
Notably,
AUC
reached
0.912
with
combination
five
biomarkers
(NEV
signatures,
CEA,
CA199)
differentiate
HC,
diagnostic
accuracy
further
increased
machine
learning
(ML)-based
ensemble
classification
system.
Therefore,
developed
is
valuable
platform
may
have
use
diagnosis.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Окт. 8, 2024
Liquid
biopsy
is
a
minimally
invasive
method
that
uses
biofluid
samples
instead
of
tissue
for
cancer
diagnosis.
Exosomes
are
small
extracellular
vesicles
secreted
by
donor
cells
and
act
as
mediators
intercellular
communication
in
human
health
disease.
Due
to
their
important
roles,
exosomes
have
been
considered
promising
biomarkers
liquid
biopsy.
However,
traditional
methods
exosome
isolation
cargo
detection
time-consuming
inefficient,
limiting
practical
application.
In
the
past
decades,
many
new
strategies,
such
microfluidic
chips,
nanowire
arrays
electrochemical
biosensors,
proposed
achieve
rapid,
accurate
high-throughput
analysis
exosomes.
this
review,
we
discussed
about
advance
exosome-based
technology,
including
isolation,
enrichment,
approaches.
The
comparison
currently
available
also
included.
Finally,
summarized
advantages
limitations
present
strategies
further
gave
perspective
future
translational
use.
International Journal of Nanomedicine,
Год журнала:
2024,
Номер
Volume 19, С. 8211 - 8236
Опубликована: Авг. 1, 2024
Hypoxia,
as
a
prominent
feature
of
the
tumor
microenvironment,
has
profound
impact
on
multicomponent
changes
within
this
environment.
Under
hypoxic
conditions,
malignant
phenotype
cells,
variety
cell
types
well
intercellular
communication
and
material
exchange,
undergo
complex
alterations.
These
provide
significant
prospects
for
exploring
mechanisms
development
under
different
microenvironmental
conditions
devising
therapeutic
strategies.
Exosomes
secreted
by
cells
stromal
are
integral
components
serving
crucial
mediators
have
consequently
garnered
increasing
attention
from
researchers.
This
review
focuses
which
promote
release
exosomes
alter
their
encapsulated
contents.
It
also
examines
effects
derived
immune
other
microenvironment.
Additionally,
we
summarize
current
research
progress
potential
clinical
applications
propose
future
directions
in
field.
