Bacterial cGAS senses a viral RNA to initiate immunity DOI Creative Commons
Dalton V. Banh, Cameron G. Roberts, Adrián Morales-Amador

и другие.

Nature, Год журнала: 2023, Номер 623(7989), С. 1001 - 1008

Опубликована: Ноя. 15, 2023

Cyclic oligonucleotide-based antiphage signalling systems (CBASS) protect prokaryotes from viral (phage) attack through the production of cyclic oligonucleotides, which activate effector proteins that trigger death infected host1,2. How bacterial cyclases recognize phage infection is not known. Here we show staphylococcal phages produce a structured RNA transcribed terminase subunit genes, termed CBASS-activating bacteriophage (cabRNA), binds to positively charged surface CdnE03 cyclase and promotes synthesis dinucleotide cGAMP CBASS immune response. Phages escape defence harbour mutations lead generation longer form cabRNA cannot CdnE03. As mammalian OAS1 also double-stranded during interferon response, our results reveal conserved mechanism for activation innate antiviral pathways.

Язык: Английский

NLR receptors in plant immunity: making sense of the alphabet soup DOI Creative Commons
Mauricio P. Contreras, Daniel Lüdke, Hsuan Pai

и другие.

EMBO Reports, Год журнала: 2023, Номер 24(10)

Опубликована: Авг. 21, 2023

Plants coordinately use cell-surface and intracellular immune receptors to perceive pathogens mount an response. Intracellular events of pathogen recognition are largely mediated by the nucleotide binding leucine rich-repeat (NLR) classes. Upon perception, NLRs trigger a potent broad-spectrum reaction, usually accompanied form programmed cell death termed hypersensitive Some plant act as multifunctional singleton which combine detection signaling. However, can also function in higher order pairs networks functionally specialized interconnected receptors. In this article, we cover basic aspects NLR biology with emphasis on networks. We highlight some recent advances structure, function, activation discuss emerging topics such modulator NLRs, suppression bioengineering. Multi-disciplinary approaches required disentangle how these receptor evolve. Answering questions holds potential deepen our understanding system unlock new era disease resistance breeding.

Язык: Английский

Процитировано

59
A conserved family of immune effectors cleaves cellular ATP upon viral infection DOI Creative Commons
François Rousset, Erez Yirmiya,

Shahar Nesher

и другие.

Cell, Год журнала: 2023, Номер 186(17), С. 3619 - 3631.e13

Опубликована: Авг. 1, 2023

Язык: Английский

Процитировано

48
Plant and prokaryotic TIR domains generate distinct cyclic ADPR NADase products DOI Creative Commons
Adam M. Bayless, Sisi Chen, Sam C. Ogden

и другие.

Science Advances, Год журнала: 2023, Номер 9(11)

Опубликована: Март 17, 2023

Toll/interleukin-1 receptor (TIR) domain proteins function in cell death and immunity. In plants bacteria, TIR domains are often enzymes that produce isomers of cyclic adenosine 5′-diphosphate–ribose (cADPR) as putative immune signaling molecules. The identity functional conservation cADPR isomer signals is unclear. A previous report found a plant could cross-activate the prokaryotic Thoeris TIR–immune system, suggesting TIR-immune signals. Here, we generate autoactive TIRs test converse hypothesis: Do also immunity? Using planta vitro assays, find overlapping sets further clarify how activate system via producing 3′cADPR. This study demonstrates requirements for systems distinct across kingdoms diversity small-molecule products.

Язык: Английский

Процитировано

46
CBASS to cGAS-STING: The Origins and Mechanisms of Nucleotide Second Messenger Immune Signaling DOI
Kailey M. Slavik, Philip J. Kranzusch

Annual Review of Virology, Год журнала: 2023, Номер 10(1), С. 423 - 453

Опубликована: Июнь 29, 2023

Host defense against viral pathogens is an essential function for all living organisms. In cell-intrinsic innate immunity, dedicated sensor proteins recognize molecular signatures of infection and communicate to downstream adaptor or effector activate immune defense. Remarkably, recent evidence demonstrates that much the core machinery immunity shared across eukaryotic prokaryotic domains life. Here, we review a pioneering example evolutionary conservation in immunity: animal cGAS-STING (cyclic GMP-AMP synthase-stimulator interferon genes) signaling pathway its ancestor bacteria, CBASS nucleotide-based antiphage system) We discuss unique mechanism by which cGLRs (cGAS-like receptors) bacterial CD-NTases (cGAS/dinucleotide-cyclase Vibrio (DncV)-like nucleotidyltransferases) these pathways link pathogen detection with activation using nucleotide second messenger signals. Comparing biochemical, structural, mechanistic details cGAS-STING, cGLR signaling, CBASS, highlight emerging questions field examine pressures may have shaped origins antiviral

Язык: Английский

Процитировано

43
Evolutionary trajectory of pattern recognition receptors in plants DOI Creative Commons
Bruno Pok Man Ngou, Michele Wyler, Marc W. Schmid

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Фев. 1, 2024

Abstract Cell-surface receptors play pivotal roles in many biological processes, including immunity, development, and reproduction, across diverse organisms. How cell-surface evolve to become specialised different processes remains elusive. To shed light on the immune-specificity of receptors, we analyzed more than 200,000 genes encoding from 350 genomes traced evolutionary origin immune-specific leucine-rich repeat receptor-like proteins (LRR-RLPs) plants. Surprisingly, discovered that motifs crucial for co-receptor interaction LRR-RLPs are closely related those LRR-receptor-like kinase (RLK) subgroup Xb, which perceives phytohormones primarily governs growth development. Functional characterisation further reveals initiate immune responses through their juxtamembrane transmembrane regions, while LRR-RLK-Xb members regulate development cytosolic domains. Our data suggest involved immunity share a common origin. After diversification, ectodomains, juxtamembrane, transmembrane, regions have either diversified or stabilised recognise ligands activate differential downstream responses. work mechanism by plants perceive signals appropriate rapidly changing environment.

Язык: Английский

Процитировано

39
Inhibitors of bacterial immune systems: discovery, mechanisms and applications DOI
David Mayo-Muñoz, Rafael Pinilla‐Redondo, Sarah Camara-Wilpert

и другие.

Nature Reviews Genetics, Год журнала: 2024, Номер 25(4), С. 237 - 254

Опубликована: Янв. 30, 2024

Язык: Английский

Процитировано

33
Accumulation of defense systems in phage-resistant strains of Pseudomonas aeruginosa DOI Creative Commons
Ana Rita Costa, Daan F. van den Berg, Jelger Q. Esser

и другие.

Science Advances, Год журнала: 2024, Номер 10(8)

Опубликована: Фев. 23, 2024

Prokaryotes encode multiple distinct anti-phage defense systems in their genomes. However, the impact of carrying a multitude on phage resistance remains unclear, especially clinical context. Using collection antibiotic-resistant strains Pseudomonas aeruginosa and broad panel phages, we demonstrate that contribute substantially to defining host range overall scales with number bacterial genome. We show many individual target specific genera complementary specificities co-occur P. genomes likely provide benefits phage-diverse environments. Overall, phage-resistant phenotypes at least 19 exist populations clinical, strains.

Язык: Английский

Процитировано

27
Conservation and similarity of bacterial and eukaryotic innate immunity DOI
Hannah E. Ledvina, Aaron T. Whiteley

Nature Reviews Microbiology, Год журнала: 2024, Номер 22(7), С. 420 - 434

Опубликована: Фев. 28, 2024

Язык: Английский

Процитировано

24
The growing repertoire of phage anti-defence systems DOI Creative Commons

Khalimat Murtazalieva,

Andre Mu,

Aleksandra Petrovskaya

и другие.

Trends in Microbiology, Год журнала: 2024, Номер 32(12), С. 1212 - 1228

Опубликована: Июнь 5, 2024

The biological interplay between phages and bacteria has driven the evolution of phage anti-defence systems (ADSs), which evade bacterial defence mechanisms. These ADSs bind inhibit host proteins, add covalent modifications deactivate degrade or sequester signalling molecules utilised by systems, synthesise restore essential depleted defences, to avoid recognition. Overall, 145 have been characterised date. counteract 27 152 different families, we hypothesise that many more are yet be discovered. We discuss high-throughput approaches (computational experimental) indispensable for discovering new limitations these approaches. A comprehensive characterisation is critical understanding phage–host developing clinical applications, such as treatment multidrug-resistant infections.

Язык: Английский

Процитировано

17
TIR signaling activates caspase-like immunity in bacteria DOI
François Rousset, Ilya А. Osterman, Tali Scherf

и другие.

Science, Год журнала: 2025, Номер 387(6733), С. 510 - 516

Опубликована: Янв. 30, 2025

Caspase family proteases and Toll/interleukin-1 receptor (TIR)-domain proteins have central roles in innate immunity regulated cell death humans. We describe a bacterial immune system comprising both caspase-like protease TIR-domain protein. found that the TIR protein, once it recognizes phage invasion, produces previously unknown signaling molecule adenosine 5′-diphosphate-cyclo[N7:1′′]-ribose (N7-cADPR). This specifically activates protease, which then indiscriminately degrades cellular to halt replication. The TIR-caspase defense system, we denote as type IV Thoeris, is abundant bacteria efficiently protects against propagation. Our study highlights diversity of TIR-produced molecules demonstrates by caspase an ancient mechanism immunity.

Язык: Английский

Процитировано

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