Journal of Hazardous Materials,
Год журнала:
2022,
Номер
431, С. 128563 - 128563
Опубликована: Фев. 24, 2022
The
insecticide
carbaryl
is
commonly
found
in
indirectly
exposed
freshwater
ecosystems
at
low
concentrations
considered
safe
for
fish
communities.
In
this
study,
we
showed
that
after
only
24
h
of
exposure
to
environmental
(0.066–660
ng/L),
zebrafish
larvae
exhibit
impairments
essential
behaviours.
Interestingly,
the
observed
behavioural
effects
induced
by
were
acetylcholinesterase-independent.
To
elucidate
molecular
initiating
event
resulted
effects,
silico
predictions
followed
vitro
validation.
We
identified
two
target
proteins
potentially
interacted
with
carbaryl,
α2B
adrenoceptor
(ADRA2B)
and
serotonin
2B
receptor
(HTR2B).
Using
a
pharmacological
approach,
then
tested
hypothesis
had
antagonistic
interactions
both
receptors.
Similar
yohimbine
SB204741,
which
are
prototypic
antagonists
ADRA2B
HTR2B,
respectively,
increased
heart
rate
larvae.
When
compared
24-h
these
those
high
degree
similarity
was
found.
These
results
strongly
suggest
antagonism
HTR2B
leads
adverse
outcomes
have
undergone
environmentally
relevant
levels
carbaryl.
Neuron,
Год журнала:
2021,
Номер
109(6), С. 918 - 937
Опубликована: Фев. 6, 2021
The
superior
colliculus
is
a
conserved
sensorimotor
structure
that
integrates
visual
and
other
sensory
information
to
drive
reflexive
behaviors.
Although
the
evidence
for
this
strong
compelling,
number
of
experiments
reveal
role
in
behaviors
usually
associated
with
cerebral
cortex,
such
as
attention
decision-making.
Indeed,
addition
collicular
outputs
targeting
brainstem
regions
controlling
movements,
also
has
ascending
projections
linking
it
forebrain
structures
including
basal
ganglia
amygdala,
highlighting
fact
colliculus,
its
vast
inputs
outputs,
can
influence
processing
throughout
neuraxis.
Today,
modern
molecular
genetic
methods
combined
sophisticated
behavioral
assessments
have
potential
make
significant
breakthroughs
our
understanding
evolution
conservation
neuronal
cell
types
circuits
give
rise
simple
complex
International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(12), С. 6647 - 6647
Опубликована: Июнь 14, 2022
To
discover
new
molecules
or
review
the
biological
activity
and
toxicity
of
therapeutic
substances,
drug
development,
research
relies
on
robust
systems
to
obtain
reliable
results.
Phenotype-based
screenings
can
transpose
organism’s
compensatory
pathways
by
adopting
multi-target
strategies
for
treating
complex
diseases,
zebrafish
emerged
as
an
important
model
biomedical
screenings.
Zebrafish’s
clear
correlation
between
neuro-anatomical
physiological
features
behavior
is
very
similar
that
verified
in
mammals,
enabling
construction
relevant
experimental
models
neurological
disorders
research.
Zebrafish
presents
highly
conserved
are
found
higher
vertebrates,
including
along
with
a
behavioral
repertoire.
Moreover,
it
sensitive
pharmacological/environmental
manipulations,
these
phenotypes
detected
both
larvae
adults.
These
advantages
align
3Rs
concept
qualify
powerful
tool
pre-clinical
trials.
This
highlights
domains
studied
their
neurotransmitter
summarizes
currently
used
techniques
evaluate
quantify
laboratory
studies.
Environment International,
Год журнала:
2023,
Номер
172, С. 107745 - 107745
Опубликована: Янв. 10, 2023
As
a
substitute
for
polybrominated
diphenyl
ethers
(PBDEs),
organophosphate
flame
retardant
triphenyl
phosphate
(TPhP)
is
widely
used
in
our
daily
products
and
diffusely
exists
living
surroundings,
but
there
paucity
of
information
concerning
its
neurodevelopmental
toxicity.
Herein,
we
investigated
the
effects
TPhP
exposure
on
developmental
parameters,
locomotor
behavior,
oxidative
stress,
apoptosis
transcriptional
levels
zebrafish
at
different
stages,
so
as
to
explore
neural
development
underlying
molecular
mechanisms.
concentration
gradient
reduced
survival
rate,
hatchability,
heart
body
length
eye
distance
embryos/larvae,
caused
malformations
larvae.
also
leads
abnormal
behaviors,
such
swimming
speed,
impaired
panic
avoidance
reflex
high
light
stimulation.
ROS
accumulation
96
hpf
larvae
induced
Nrf2-antioxidant
response
zebrafish.
In
addition,
further
activated
mitochondrial
signaling
pathways,
which
affected
region,
resulting
visual
impairment.
Neurodevelopmental
(mbpa,
syn2a,
foxo3a
pax6a),
Retinoid
acid
metabolism
(cyp26a1,
raraa,
rbp5,
rdh1,
crabp1a
rbp2a)
apoptosis-related
genes
(bcl2a,
baxa
casp9)
revealed
mechanism
behavior
phenotypic
symptoms,
indicated
that
are
more
sensitive
than
7
dpf
Thus,
present
study,
neurotoxic
early
life
stages
zebrafish,
impairments
by
attributed
co-regulation
visuomotor
dysfunction
neuro-related
genes.
These
results
suggest
safety
organisms
even
humans
needs
be
studied.
Neuroscience Bulletin,
Год журнала:
2022,
Номер
38(12), С. 1519 - 1540
Опубликована: Апрель 28, 2022
Abstract
The
superior
colliculus
(SC),
one
of
the
most
well-characterized
midbrain
sensorimotor
structures
where
visual,
auditory,
and
somatosensory
information
are
integrated
to
initiate
motor
commands,
is
highly
conserved
across
vertebrate
evolution.
Moreover,
cell-type-specific
SC
neurons
integrate
afferent
signals
within
local
networks
generate
defined
output
related
innate
cognitive
behaviors.
This
review
focuses
on
recent
progress
in
understanding
phenotypic
diversity
amongst
their
intrinsic
circuits
long-projection
targets.
We
further
describe
relevant
neural
specific
cell
types
relation
behavioral
outputs
functions.
systematic
delineation
organization,
types,
connections
put
into
context
species
as
these
depend
upon
laminar
architecture.
we
focus
circuitry
involving
saccadic
eye
movement,
Overall,
provides
insight
functioning
represents
a
basis
for
pathology
associated
with
dysfunction.
Frontiers in Neuroscience,
Год журнала:
2023,
Номер
17
Опубликована: Апрель 17, 2023
One
of
the
holy
grails
neuroscience
is
to
record
activity
every
neuron
in
brain
while
an
animal
moves
freely
and
performs
complex
behavioral
tasks.
While
important
steps
forward
have
been
taken
recently
large-scale
neural
recording
rodent
models,
single
resolution
across
entire
mammalian
remains
elusive.
In
contrast
larval
zebrafish
offers
great
promise
this
regard.
Zebrafish
are
a
vertebrate
model
with
substantial
homology
brain,
but
their
transparency
allows
whole-brain
recordings
genetically-encoded
fluorescent
indicators
at
single-neuron
using
optical
microscopy
techniques.
Furthermore
begin
show
repertoire
natural
behavior
from
early
age,
including
hunting
small,
fast-moving
prey
visual
cues.
Until
work
address
bases
these
behaviors
mostly
relied
on
assays
where
fish
was
immobilized
under
microscope
objective,
stimuli
such
as
were
presented
virtually.
However
significant
progress
has
made
developing
imaging
techniques
for
which
not
immobilized.
Here
we
discuss
recent
advances,
focusing
particularly
based
light-field
microscopy.
We
also
draw
attention
several
outstanding
issues
remain
be
addressed
increase
ecological
validity
results
obtained.
Annual Review of Vision Science,
Год журнала:
2023,
Номер
9(1), С. 361 - 383
Опубликована: Апрель 11, 2023
The
superior
colliculus
(SC)
is
a
subcortical
brain
structure
that
relevant
for
sensation,
cognition,
and
action.
In
nonhuman
primates,
rich
history
of
studies
has
provided
unprecedented
detail
about
this
structure's
role
in
controlling
orienting
behaviors;
as
result,
the
primate
SC
become
primarily
regarded
motor
control
structure.
However,
other
species,
also
highly
visual
structure:
A
fraction
its
inputs
retinal
complemented
by
from
cortical
areas,
including
primary
cortex.
Motivated
this,
recent
investigations
are
revealing
pattern
analysis
capabilities
SC,
placing
an
ideal
position
to
guide
movements.
anatomical
proximity
both
early
final
apparatuses,
well
ascending
feedback
projections
cortex,
affirms
important
active
perception.
