World Journal of Gastroenterology,
Год журнала:
2025,
Номер
31(11)
Опубликована: Март 12, 2025
BACKGROUND
Glycolysis
provides
growth
advantages
and
leads
to
drug
resistance
in
colorectal
cancer
(CRC)
cells.
SIRT1,
an
NAD+-dependent
deacetylase,
regulates
various
cellular
processes,
its
upregulation
results
antitumor
effects.
This
study
investigated
the
role
of
SIRT1
metabolic
reprogramming
oxaliplatin
CRC
AIM
To
investigate
overcoming
METHODS
We
performed
transcriptome
sequencing
human
parental
cells
oxaliplatin-resistant
identify
differentially
expressed
genes.
Key
regulators
were
identified
via
LINCS
database.
NAD+
levels
measured
by
flow
cytometry,
effects
on
sensitivity
assessed
MTS
assays,
colony
formation
xenograft
models.
Glycolytic
function
was
using
Western
blot
Seahorse
assays.
RESULTS
Salermide,
a
inhibitor,
as
candidate
compound
that
enhances
resistance.
In
cells,
downregulated,
whereas
γH2AX
PARP
upregulated.
activation
led
depletion
inhibition,
which
reversed
inhibitor
treatment.
The
increase
expression
overcame
resistance,
while
inhibition
increased
glycolysis,
inhibited
glycolysis
resistant
characterized
reduced
glycolytic
enzymes
PKM2
LDHA,
well
decreased
extracellular
acidification
rate.
shikonin
induced
inhibition.
CONCLUSION
is
due
activation,
turn
increases
glycolysis.
Restoring
reverses
offering
promising
therapeutic
strategy
overcome
Cell Communication and Signaling,
Год журнала:
2024,
Номер
22(1)
Опубликована: Фев. 12, 2024
Abstract
Cancer
treatment
faces
many
hurdles
and
resistance
is
one
among
them.
Anti-cancer
strategies
are
evolving
due
to
innate
acquired
capacity,
governed
by
genetic,
epigenetic,
proteomic,
metabolic,
or
microenvironmental
cues
that
ultimately
enable
selected
cancer
cells
survive
progress
under
unfavorable
conditions.
Although
the
mechanism
of
drug
being
widely
studied
generate
new
target-based
drugs
with
better
potency
than
existing
ones.
However,
broader
flexibility
in
resistance,
advanced
therapeutic
options
efficacy
need
be
explored.
Combination
therapy
an
alternative
a
success
rate
though
risk
amplified
side
effects
commonplace.
Moreover,
recent
groundbreaking
precision
immune
ways
overcome
has
revolutionized
anticancer
greater
extent
only
limitation
individual-specific
needs
further
attention.
This
review
will
focus
on
challenges
opted
withstand
current
therapies
at
molecular
level
also
highlights
emerging
-like
immunological,
stem
cell-based
may
prove
have
potential
challenge
problem
resistance.
Chemical Reviews,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 14, 2025
Ferroptosis,
an
iron-dependent
form
of
regulatory
cell
death,
has
garnered
significant
interest
as
a
therapeutic
target
in
cancer
treatment
due
to
its
distinct
characteristics,
including
lipid
peroxide
generation
and
redox
imbalance.
However,
clinical
application
oncology
is
currently
limited
by
issues
such
suboptimal
efficacy
potential
off-target
effects.
The
advent
nanotechnology
provided
new
way
for
overcoming
these
challenges
through
the
development
activatable
magnetic
nanoparticles
(MNPs).
These
innovative
MNPs
are
designed
improve
specificity
ferroptosis
induction.
This
Review
delves
into
chemical
biological
principles
guiding
design
ferroptosis-based
therapies
imaging-guided
therapies.
It
discusses
mechanisms
attributes
ferroptosis,
composition
MNPs,
their
mechanism
action
inducers,
integration
with
advanced
imaging
techniques
monitoring.
Additionally,
we
examine
convergence
other
strategies,
chemodynamic
therapy,
photothermal
photodynamic
sonodynamic
immunotherapy,
within
context
nanomedicine
strategies
utilizing
MNPs.
highlights
multifunctional
surpass
limitations
conventional
treatments,
envisioning
future
drug-resistance-free,
precision
diagnostics
treating
recalcitrant
cancers.
Scientific Reports,
Год журнала:
2023,
Номер
13(1)
Опубликована: Сен. 1, 2023
Abstract
Oxaliplatin
is
widely
used
in
chemotherapy
for
colorectal
cancer
(CRC),
but
its
sensitivity
has
become
a
major
obstacle
to
limiting
efficacy.
Many
literatures
reported
that
Nrf2
activation
promoted
tumor
chemoresistance.
In
this
study,
we
explored
the
role
and
mechanism
of
inhibition
oxaliplatin-based
chemosensitivity
CRC.
vitro
experiments,
applied
4-octyl
itaconate
(4-OI)
activate
Nrf2,
lentivirus
knock
down
CRC
cell
lines.
By
measuring
viability,
colony
formation,
apoptosis,
reactive
oxygen
species
production,
western
blot,
found
oxaliplatin
lobaplatin
suppressed
growth
HCT-116
LOVO
cells
dose-dependent
manner,
expression
Nrf2.
4-OI,
an
activator,
reduced
sensibility
lobaplatin,
while
knockdown
lobaplatin.
Through
public
databases,
GPX4
normal
tissues
was
lower
compared
with
CRC,
high
predicted
poor
prognosis.
Meanwhile,
vitro.
The
enhanced
effects
reduce
GSH
content,
increase
MDA
which
oxaliplatin-induced
ferroptosis.
Subsequently,
GSDME-N,
induced
LDH,
IL-1β,
TNF-a
release,
aggravated
occurrence
GSMDE-mediated
pyroptosis.
Finally,
on
HCT116
xenograft
vivo.
Thus,
our
study
showed
improved
by
promoting
ferroptosis
pyroptosis,
provided
new
target
overcoming
chemoresistance
Cell Reports Medicine,
Год журнала:
2023,
Номер
4(10), С. 101231 - 101231
Опубликована: Окт. 1, 2023
Neoadjuvant
chemotherapy
(NAC)
for
rectal
cancer
(RC)
shows
promising
clinical
response.
The
modulation
of
the
tumor
microenvironment
(TME)
by
NAC
and
its
association
with
therapeutic
response
remain
unclear.
Here,
we
use
single-cell
RNA
sequencing
spatial
transcriptome
to
examine
cell
dynamics
in
29
patients
RC,
who
are
sampled
pairwise
before
after
treatment.
We
construct
a
high-resolution
cellular
dynamic
landscape
remodeled
their
associations
markedly
reshapes
populations
cancer-associated
fibroblasts
(CAFs),
which
is
strongly
associated
CAF
subsets
regulate
TME
through
recruitment
crosstalk
activate
immunity
suppress
progression
multiple
cytokines,
including
CXCL12,
SLIT2,
DCN.
In
contrast,
epithelial-mesenchymal
transition
malignant
cells
upregulated
CAF_FAP
MIR4435-2HG
induction,
resulting
worse
outcomes.
Our
study
demonstrates
that
inhibits
modulates
remodeling
CAFs.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(9), С. 7922 - 7922
Опубликована: Апрель 27, 2023
Colorectal
cancer
(CRC)
is
the
third
most
common
worldwide,
and
metastatic
CRC
a
fatal
disease.
The
CRC-affected
tissues
show
several
molecular
markers
that
could
be
used
as
fresh
strategy
to
create
newer
methods
of
treating
condition.
liver
peritoneum
are
where
metastasis
occurs
frequently.
Once
tumor
has
metastasized
liver,
peritoneal
carcinomatosis
frequently
regarded
disease's
final
stage.
However,
nearly
50%
patients
with
do
not
have
metastases.
New
diagnostic
therapeutic
approaches
must
developed
due
poor
response
present
treatment
choices
in
advanced
stages
necessity
an
accurate
diagnosis
early
stages.
Many
unique
amazing
nanomaterials
promise
for
both
may
found
nanotechnology.
Numerous
nanoformulations,
including
carbon
nanotubes,
dendrimers,
liposomes,
silica
nanoparticles,
gold
metal-organic
frameworks,
core-shell
polymeric
nano-formulations,
nano-emulsion
systems,
among
others,
can
targeted
anticancer
drug
delivery
purposes
CRC.
Theranostic
combined
nanomedicine
been
proposed
revolutionary
approach
improve
detection
treatment.
This
review
highlights
recent
studies,
potential,
challenges
development
nanoplatforms
Colorectal
cancer
(CRC)
is
a
common
gastrointestinal
cancer,
and
even
though
oxaliplatin
chemotherapy
effective,
there
high
likelihood
of
relapse,
indicating
the
presence
oxaliplatin-resistant
CRC.
Therefore,
it
crucial
to
comprehend
molecular
mechanisms
resistance
develop
effective
strategies
counter
drug
resistance.
Numerous
studies
have
demonstrated
close
association
between
microRNAs
(miRNAs)
in
In
this
study,
we
aimed
identify
essential
exosomal
cellular
miRNA
related
CRC
cell
line
HCT-116.
The
expression
profile
cells
with
was
analyzed.
effectiveness
diagnostics
biomarker
potency
miRNAs
were
evaluated
by
receiver
operating
characteristic
(ROC)
analysis.
Target
identified,
enrichment
analysis
assessed
based
on
Gene
Ontology
(GO),
Reactome,
Human
Disease
(DO).
vitro
experiments,
HCT-116
(HCT116-OXA)
developed,
exosomes
isolated
characterized
from
HCT116-OXA
HCT116
cells.
selected
their
exosomes,
they
compared
using
quantitative
real-time
PCR.
This
study
revealed
that
combination
miR-4326,
miR-3615,
miR-7974,
miR-130b-3p,
miR-454-3p
exhibited
highest
area
under
curve
(AUC),
sensitivity,
specificity,
superior
diagnostic
predictive
performance.
displayed
reduced
early
late
apoptosis,
bypass
S
phase
arrest,
prolonged
doubling
time,
higher
IC50
parental
miR-454-3p,
miR-4326
decreased
as
sensitive
However,
significantly
miR-130b-3p
observed
low
miR-3615
or
could
predict
response
therapy.
indicates
potential
these
specific
serve
markers
for
