Medicine,
Год журнала:
2024,
Номер
103(33), С. e39350 - e39350
Опубликована: Авг. 16, 2024
Background:
Previous
researches
have
demonstrated
that
the
traditional
Chinese
medicine
could
therapeutically
treat
inflammatory
and
hypoxic
diseases
by
enhancing
functionality
of
mesenchymal
stem
cells.
However,
its
mechanism
was
not
yet
clear.
This
research
aimed
to
investigate
impact
Sijunzi
decoction
herb
monomer
ginsenoside
Rg1
on
proliferation
differentiation
human
umbilical
cord
cells
(hUC-MSCs)
explore
underlying
mechanisms.
Methods:
Different
concentrations
were
applied
differentiating
induced
hUC-MSCs.
The
CCK-8
test
utilized
evaluate
cell
activity
identify
suitable
drug
concentrations.
Alizarin
Red
staining
employed
detect
formation
calcium
nodules,
Oil
O
used
assess
lipid
droplets.
PCR
examine
gene
expression
related
osteogenic
differentiation,
adipogenic
HIF-1α
signaling
pathway
in
Western
blot
analysis
conducted
protein
HIF-1α.
ELISA
performed
measure
factors
cytokine
expression.
Biochemical
assays
changes
oxidative
stress
indicators.
Results:
both
a
dose-dependent
promotion
hUC-MSC
proliferation.
significantly
increased
genes
proteins
relevant
osteogenesis,
such
as
osterix,
osteocalcin,
RUNX2,
osteopontin,
activated
(
P
<
.05).
Similar
effects
observed
at
level
after
treatment
with
Rg1.
Simultaneously,
reduced
secretion
pro-inflammatory
cytokines
TNF-α,
IL-6,
IL-1β,
while
increasing
anti-inflammatory
IL-10
during
Moreover,
lowered
levels
enhanced
antioxidant
capacity
hUC-MSCs
toward
significant
>
Conclusion:
promotes
hUC-MSCs,
potentially
through
activation
modulating
microenvironment
via
reducing
inflammation
levels.
might
be
involved
this
process.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(5), С. 2452 - 2452
Опубликована: Фев. 20, 2024
Plant-derived
nanovesicles
have
been
considered
interesting
in
medicine
for
their
breakthrough
biological
effects,
including
those
relevant
to
wound
healing.
However,
tomato-derived
(TDNVs)
not
studied
effects
on
closure
yet.
TDNVs
were
isolated
from
Solanum
lycopersicum
(var.
Piccadilly)
ripe
tomatoes
by
ultracentrifugation.
Extract
(collected
during
the
isolation
procedure)
and
NVs
(pellet)
characterized
transmission
electron
microscopy
laser
Doppler
electrophoresis.
Wound
healing
presence
of
or
was
analyzed
a
scratch
assay
with
monocultures
human
keratinocytes
(HUKE)
NIH-3T3
mouse
fibroblasts.
Cell
proliferation
migration
MTT
agarose
spot
assay,
respectively.
The
vesicles
NV
samples
nanosized
similar
mean
diameter
115
nm
130
nm,
Both
had
already
accelerated
injured
HUKE
6
h
post-injury.
Although
neither
sample
exerted
cytotoxic
effect
fibroblasts,
they
did
augment
cell
proliferation.
increased
both
types.
may
accelerate
increasing
keratinocyte
fibroblast
migration.
These
results
indicate
potential
therapeutic
usefulness
treatment
chronic
hard-to-heal
ulcers.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Май 19, 2024
Abstract
Symbiotic
microbial
communities
are
crucial
for
human
health,
and
dysbiosis
is
associated
with
various
diseases.
Plant-derived
nanovesicles
(PDNVs)
have
a
lipid
bilayer
structure
contain
lipids,
metabolites,
proteins,
RNA.
They
offer
unique
advantages
in
regulating
community
homeostasis
treating
diseases
related
to
compared
traditional
drugs.
On
the
one
hand,
lipids
on
PDNVs
serve
as
primary
substances
that
mediate
specific
recognition
uptake
by
bacteria.
other
due
multifactorial
nature
of
PDNVs,
they
potential
enhance
growth
survival
beneficial
bacterial
while
simultaneously
reducing
pathogenicity
harmful
In
addition,
capacity
modulate
metabolism,
thus
facilitating
establishment
harmonious
equilibrium
promoting
stability
within
microbiota.
These
remarkable
attributes
make
promising
therapeutic
approach
conditions,
including
periodontitis,
inflammatory
bowel
disease,
skin
infection
However,
challenges
such
consistency,
isolation
methods,
storage
need
be
addressed
before
clinical
application.
This
review
aims
explore
value
provide
recommendations
their
use
novel
agents
health
protection.
Graphical
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 23, 2025
Nonalcoholic
fatty
liver
disease
(NAFLD)
represents
an
increasing
public
health
concern.
The
underlying
pathophysiological
mechanisms
of
NAFLD
remains
unclear,
and
as
a
result,
there
is
currently
no
specific
therapy
for
this
condition.
However,
recent
studies
focus
on
extracellular
vesicles
(EVs)
novelty
in
their
role
cellular
communication.
An
imbalance
the
gut
microbiota
composition
may
contribute
to
progression
NAFLD,
making
gut-liver
axis
promising
target
therapeutic
strategies.
This
review
aims
provide
comprehensive
overview
EVs
NAFLD.
Additionally,
exosome-like
nanovesicles
derived
from
plants
(PELNs)
probiotics-derived
(postbiotics)
have
demonstrated
potential
re-establish
intestinal
equilibrium
modulate
microbiota,
thus
offering
alleviate
via
axis.
Further
research
needed
using
multiple
omics
approaches
comprehensively
characterize
cargo
including
protein,
metabolites,
genetic
material
packaged,
biological
activities
diverse
microbes
plants.
Frontiers in Cell and Developmental Biology,
Год журнала:
2025,
Номер
13
Опубликована: Фев. 27, 2025
Periodontitis
is
a
chronic
inflammatory
disease
affecting
the
supporting
tissues
of
teeth
and
has
emerged
as
global
public
health
issue.
Current
therapies
primarily
address
pathogenic
factors
alleviate
symptoms,
with
limited
options
available
for
complete
restoration
reconstruction
already
absorbed
periodontal
bone
tissue.
In
this
study,
we
developed
nanotherapeutic
strategy
utilizing
fusion
nanovesicles
(FVs)
to
modulate
microenvironment
create
regenerative
niche
ligament
stem
cells
(PDLSCs),
which
play
crucial
role
in
tissue
repair.
The
FVs
are
composed
Scutellaria
baicalensis
(SBNVs)
anti-Porphyromonas
gingivalis
(P.
gingivalis)
anti-inflammatory
properties,
combined
PDLSC
membrane-derived
genetically
engineered
express
TNFR1.
These
preserved
biological
activity
SBNVs
immunomodulatory
function
PDLSCs.
Additionally,
effectively
captured
cleared
TNF-α
from
through
Moreover,
alleviated
response
PDLSCs
induced
by
P.
gingivalis-LPS
(Pg-LPS)
TNF-α,
restoring
their
proliferation,
migration,
osteogenic
differentiation
capabilities.
Hence,
holds
great
potential
treating
periodontitis.
Biomarker Research,
Год журнала:
2025,
Номер
13(1)
Опубликована: Март 12, 2025
Abstract
The
cyclic
GMP-AMP
synthase
(cGAS)-stimulator
interferon
genes
(STING)
signaling
pathway
plays
a
crucial
role
in
activating
innate
and
specific
immunity
anti-tumor
immunotherapy.
As
the
major
infiltrating
cells
tumor
microenvironment
(TME),
tumor-associated
macrophages
(TAMs)
could
be
polarized
into
either
M1
or
pro-tumor
M2
types
based
on
various
stimuli.
Accordingly,
targeted
reprogramming
TAMs
to
restore
immune
balance
shows
promise
as
an
effective
strategy.
In
this
review,
we
aim
target
cGAS-STING
for
enhance
We
investigated
double-edged
sword
effects
of
regulating
TME.
regulative
roles
its
impact
TME
were
further
revealed.
More
importantly,
several
strategies
targeting
designed
enhancing
Taken
together,
might
promising
strategy
Journal of Nanobiotechnology,
Год журнала:
2025,
Номер
23(1)
Опубликована: Апрель 1, 2025
Ulcerative
colitis
(UC)
is
an
inflammatory
bowel
disease
(IBD),
accompanied
by
intense
inflammation,
oxidative
stress,
and
intestinal
microbiota
dysbiosis.
Current
treatments
using
chemotherapeutic
drugs
or
immunosuppressants
have
limited
effectiveness
side
effects.
Therefore,
the
development
of
safe,
effective,
multi-targeting
therapies
for
IBD
great
importance.
Momordica
charantia
exhibits
antioxidant,
anti-inflammatory,
microbiota-regulating
properties,
suggesting
that
charantia-derived
extracellular
vesicles
(MCEVs)
potential
UC
management.
We
extracted
MCEVs
differential
centrifugation
density
gradient
centrifugation.
The
results
showed
possessed
high
purity,
even
particle
size,
excellent
stability.
In
vitro,
were
shown
to
inhibit
macrophage
responses,
scavenge
reactive
oxygen
species
(ROS),
protect
cells
from
damage.
Transcriptomics
analysis
revealed
may
alleviate
mitochondria-dependent
apoptosis
safeguarding
integrity
mitochondrial
structure
regulating
expression
apoptosis-related
proteins.
Furthermore,
all
components
contributed
their
pharmacological
activity.
vivo,
had
better
retention
in
inflamed
colon
significantly
alleviated
through
a
comprehensive
renovation
microenvironment.
These
findings
suggested
own
considerable
as
natural
nanotherapeutics
treatment.
Food & Function,
Год журнала:
2024,
Номер
15(14), С. 7641 - 7657
Опубликована: Янв. 1, 2024
Edible
plant-derived
nanovesicles
(ePDNs)
have
shown
potential
as
a
non-pharmacological
option
for
inflammatory
bowel
disease
(IBD)
by
maintaining
gut
health
and
showing
anti-inflammatory
effects.
However,
the
effects
of
Allium
tuberosum-derived
(ADNs)
on
colitis
not
been
studied
to
date.
Here,
we
extracted
exosome-like
from
tuberosum
investigated
whether
they
an
effect
in
RAW
264.7
cells
mice.
The
results
showed
that
ADNs
reduced
elevated
levels
factors
such
IL-1β,
IL-6,
TNF-α,
NF-κB
pathway-related
proteins
consequence
lipopolysaccharide
(LPS)
stimulation
cells.
Furthermore,
our
mouse
experiments
demonstrated
could
ameliorate
dextran
sulfate
sodium
(DSS)-induced
symptoms
(e.g.,
increased
activity
index
score,
intestinal
permeability,
histological
appearance).
Additionally,
counteracted
DSS-induced
downregulating
expression
serum
amyloid
A
(SAA),
TNF-α
increasing
tight
junction
(ZO-1
occludin)
cytokine
IL-10.
16S
rRNA
gene
sequencing
ADN
intervention
restored
microbial
composition,
which
was
similar
DSS
non-treated
group,
decreasing
ratio
Firmicutes
Bacteroidetes
relative
abundance
Proteobacteria.
induced
acetic
acid
production
along
with
increase
Lactobacillus.
Overall,
findings
suggest
supplementation
has
crucial
role
is
novel
preventive
therapy
IBD.
