AGD1/USP10/METTL13 complexes enhance cancer stem cells proliferation and diminish the therapeutic effect of docetaxel via CD44 m6A modification in castration resistant prostate cancer

Journal of Experimental & Clinical Cancer Research, Год журнала: 2025, Номер 44(1)

Опубликована: Янв. 14, 2025

Abstract Background Most patients with prostate cancer inevitably progress to castration-resistant (CRPC), at which stage chemotherapeutics like docetaxel become the first-line treatment. However, chemotherapy resistance typically develops after an initial period of therapeutic efficacy. Increasing evidence indicates that stem cells confer via exosomes. This study demonstrated AGD1, derived from (PCSCs), enhanced stemness and reduced effect in CRPC. Methods Quantitative real-time PCR (qPCR) was employed determine expression levels AGD1 METTL13 mRNAs PCSCs Protein were examined using western blots dot blots. The potential functions CRPC investigated through cell proliferation assay, Transwell EdU incorporation assays, Annexin V-FITC/PI staining, sphere formation assays. To uncover underlying mechanisms RNA pull-down RIP, co-Immunoprecipitation (co-IP), mass spectrometry (MS), Methylated immunoprecipitation (MeRIP) single-base elongation ligation-based qPCR amplification method (SELECT) performed. effects on development metastasis under treatment analyzed a xenograft mouse model organoid model. Additionally, liposomal-chitosan nanocomplex drug delivery systems designed explore AGD1’s role regulating Results upregulated Downregulating sensitivity by inhibiting their stemness, reverse also being true. pull-down, combined MS, co-IP RIP binds USP10, forming complex facilitates protein accumulation USP10-induced deubiquitination. MeRIP assay SELECT revealed transcriptionally controls mRNA decay CD44 m6A methylation. this process activates pSTAT3/PI3K-AKT signaling pathway. Organoid models showed reducing Conclusions mediates apoptosis promotes enhancing tumor growth USP10/METTL13-mediated

Язык: Английский

---

Environmental pollutants and exosomes: A new paradigm in environmental health and disease

The Science of The Total Environment, Год журнала: 2024, Номер 925, С. 171774 - 171774

Опубликована: Март 18, 2024

Язык: Английский

---

The hidden architects of glioblastoma multiforme: Glioma stem cells

MedComm – Oncology, Год журнала: 2024, Номер 3(1)

Опубликована: Фев. 1, 2024

Abstract Glioblastoma multiforme (GBM), a WHO grade IV diffuse glioma, is highly aggressive brain tumor with median survival of less than year. Characterized by robust proliferation and invasion, recent studies spotlight glioma stem cells (GSCs) within GBM tumors, pivotal in development, progression, treatment resistance. This review aims to shed light on the critical role GSCs initiation progression GBM, emphasizing their contribution development resistance existing treatments. Unlike normal cells, play pathogenesis. The delves into unique characteristics GSCs, marked heightened metabolic activities distinct epigenetic transcriptional programming. Recognizing significance years, examines how presence amplifies lethal nature GBM. also critically evaluates advancements diagnostic methods therapies, which include targeting GSCs. Providing concise yet comprehensive overview, contributes insights GBM's intricate dynamics, offering potential directions for future research therapeutic strategies.

Язык: Английский

---

Tumorigenic and tumoricidal properties of exosomes in cancers; a forward look

Cell Communication and Signaling, Год журнала: 2024, Номер 22(1)

Опубликована: Фев. 15, 2024

Abstract In recent decades, emerging data have highlighted the critical role of extracellular vesicles (EVs), especially (exosomes) Exos, in progression and development several cancer types. These nano-sized are released by different cell lineages within niche maintain a suitable platform for interchange various signaling molecules paracrine manner. Based on studies, Exos can transfer oncogenic factors to other cells, alter activity immune tumor microenvironment, leading expansion cells metastasis remote sites. It has been indicated that cell-to-cell crosstalk is so complicated wide array involved this process. How which mechanisms regulate behavior non-cancer at center debate. Here, we scrutinize molecular parenchyma. Besides, tumoricidal properties from stem (SC) types discussed detail.

Язык: Английский

---

Advancements in extracellular vesicles biomanufacturing: a comprehensive overview of large-scale production and clinical research

Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 13

Опубликована: Фев. 19, 2025

Extracellular vesicles (EVs) are nano-sized, membranous structures secreted by cells into the extracellular space, have attracted considerable attention in field of biosciences for their role intercellular communication various physiological and pathological processes. Their ubiquitous presence bodily fluids cell-specific characteristics make them promising candidates as biomarkers. Additionally, ability to transport biological therapeutics across different barriers specific target underscores significant translational potential diagnostic therapeutic purposes. Significant progress has been achieved translation EVs research clinical applications, however, challenges persist large-scale production EVs, particularly areas scalable manufacturing, efficient isolation methods, drug loading techniques, advanced characterization technology. This review critically examines complex processes involved biogenesis explores recent developments production. By synthesizing knowledge from these fields, this aims provide a holistic perspective on evolving landscape its underscoring both accomplishments obstacles that lie ahead fully realizing biomedicine.

Язык: Английский

---

Breast cancer stem cells convert anti-tumor CD4+ T cells to pro-tumor T regulatory cells: Potential role of exosomal FOXP3

Cellular Immunology, Год журнала: 2025, Номер 409-410, С. 104931 - 104931

Опубликована: Фев. 20, 2025

Язык: Английский

---

Urine-derived stem cells: Promising advancements and applications in regenerative medicine and beyond

Heliyon, Год журнала: 2024, Номер 10(6), С. e27306 - e27306

Опубликована: Март 1, 2024

Currently, stem cells are a prominent focus of regenerative engineering research. However, due to the limitations commonly used cell sources, their application in therapy is often restricted experimental stage and constrained by ethical considerations. In contrast, urine-derived (USCs) offer promising advantages for clinical trials applications. The noninvasive nature collection process allows repeated retrieval within short period, making it more feasible option. Moreover, studies have shown that USCs protective effect on organs, promoting vascular regeneration, inhibiting oxidative stress, reducing inflammation various acute chronic organ dysfunctions. has also been enhanced advancements biomaterials technology, enabling better targeting controlled release capabilities. This review aims summarize current state research USCs, providing insights future applications basic settings.

Язык: Английский

---

Role of Exosomes in the Pathogenesis and Theranostic of Alzheimer’s Disease and Parkinson’s Disease

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(13), С. 11054 - 11054

Опубликована: Июль 4, 2023

Alzheimer’s disease (AD) and Parkinson’s (PD) are the most common neurodegenerative diseases (NDDs) threatening lives of millions people worldwide, including especially elderly people. Currently, due to lack a timely diagnosis proper intervention strategy, AD PD largely remain incurable. Innovative therapy highly desired. Exosomes small vesicles that present in various bodily fluids, which contain proteins, nucleic acids, active biomolecules, play crucial role intercellular communication. In recent years, exosomes pathogenesis, early diagnosis, treatment has attracted ascending attention. However, exact pathogenesis theragnostic not been fully illustrated. review, we first introduce biogenesis, components, uptake, function exosomes. Then elaborate on involvement PD. Moreover, application therapeutics is also summarized discussed. Additionally, serving as drug carriers deliver medications central nervous system specifically addressed. The potential explored, discussing their applications treatment, well current limitations. Given limitation exosomes, propose future perspectives for better utilizing NDDs. Hopefully, it would pave ways expanding biological fundamental research theranostics

Язык: Английский

---

Harnessing Lactobacillus reuteri‐Derived Extracellular Vesicles for Multifaceted Cancer Treatment

Small, Год журнала: 2024, Номер unknown

Опубликована: Окт. 18, 2024

Abstract Extracellular vesicles (EVs) have emerged as valuable biological materials for treating intractable diseases. Extensive studies are conducted on EVs derived from various cellular sources. In this study, Lactobacillus reuteri ( L. ), a probiotic, exhibit remarkable cancer therapeutic efficacy when administered orally is reported. These ‐derived (REVs) demonstrate stability in the gastrointestinal tract and exert significant anti‐tumor effects. Using A549 cells murine models, we confirmed that REVs mediate their effects by modulating apoptotic signaling pathways. Furthermore, combination of REV with drugs enhances tumor ablation induces immunogenic cell death. mouse model, oral administration encapsulating indocyanine green followed photothermal therapy led to complete elimination within 32 days. represent promising platform treatment, either independently or other therapies, depending treatment objectives.

Язык: Английский

---

Unraveling the underlying mechanisms of cancer stem cells in therapeutic resistance for optimizing treatment strategies

MedComm – Oncology, Год журнала: 2025, Номер 4(1)

Опубликована: Янв. 10, 2025

Abstract The success of cancer therapy has been significantly hampered by various mechanisms therapeutic resistance. Chief among these is the presence clonal heterogeneity within an individual tumor mass. introduction concept stem cells (CSCs)—a rare and immature subpopulation with tumorigenic potential that contributes to intratumoral heterogeneity—has deepened our understanding drug Given characteristics CSCs, such as increased drug‐efflux activity, enhanced DNA‐repair capacity, high metabolic plasticity, adaptability oxidative stress, and/or upregulated detoxifying aldehyde dehydrogenase (ALDH) enzymes, CSCs have recognized a theoretical reservoir for resistant diseases. Implicit in this recognition possibility CSC‐targeted strategies might offer breakthrough overcoming resistance patients. Herein, we summarize generation current underlying CSC‐mediated This extended knowledge progressively translated into novel anticancer enriched available options combination treatments, all which are anticipated improve clinical outcomes patients experiencing CSC‐related relapse.

Язык: Английский

---