Frontiers in Immunology,
Год журнала:
2022,
Номер
12
Опубликована: Янв. 11, 2022
Rheumatoid
arthritis
(RA)
is
a
systemic
chronic
autoinflammatory
disease,
and
the
synovial
hyperplasia,
pannus
formation,
articular
cartilage
damage
bone
matrix
destruction
caused
by
immune
system
abnormalities
are
main
features
of
RA.
The
use
Disease
Modifying
Anti-Rheumatic
Drugs
(DMARDs)
has
achieved
great
advances
in
therapy
Yet
there
still
patients
facing
problem
poor
response
to
drug
or
intolerance.
Current
methods
can
only
moderate
RA
progress,
but
cannot
stop
reverse
it
caused.
Recent
studies
have
reported
that
variety
long
non-coding
RNAs
(LncRNAs)
been
implicated
mediating
many
aspects
Understanding
mechanism
LncRNAs
therefore
critical
for
development
new
strategies
prevention
strategies.
In
this
review,
we
systematically
elucidate
biological
roles
mechanisms
action
their
Additionally,
also
highlight
potential
value
clinical
diagnosis
Stem Cell Research & Therapy,
Год журнала:
2025,
Номер
16(1)
Опубликована: Янв. 23, 2025
Articular
cartilage
injuries
can
lead
to
pain,
stiffness,
and
reduced
mobility,
may
eventually
progress
osteoarthritis
(OA).
Despite
substantial
research
efforts,
effective
therapies
capable
of
regenerating
are
still
lacking.
Mesenchymal
stromal
cells
(MSCs)
known
for
their
differentiation
immunomodulatory
capabilities,
yet
challenges
such
as
limited
survival
post-injection
inconsistent
therapeutic
outcomes
hinder
clinical
application.
Recent
evidence
suggests
that
the
beneficial
effects
MSCs
largely
mediated
by
secreted
small
extracellular
vesicles
(sEVs),
which
have
been
shown
promote
tissue
repair
reduce
inflammation.
MSC-derived
sEVs
promise
in
mitigating
degradation
chondrocyte
apoptosis,
positioning
them
a
promising
alternative
MSC-based
OA
treatment.
This
review
explores
potential
limitations
regeneration.
systematic
was
conducted
following
PRISMA
guidelines,
with
comprehensive
search
Web
Science
Scopus
databases
studies
published
between
2019
2024.
A
total
223
records
were
identified,
132
articles
assessed
eligibility
based
on
general
selection
criteria.
After
full-text
screening,
60
initially
selected,
comprising
58
vitro
40
vivo
studies.
Following
further
exclusion
specific
criteria,
33
28
from
47
scientific
papers
included
final
qualitative
synthesis.
Most
indicate
enhance
proliferation,
improve
matrix
composition,
matrix-degrading
enzymes
inflammation,
thereby
delaying
progression.
growing
body
supports
use
tool
preventing
progression,
most
reporting
structure
function.
However,
remain
optimizing
standardizing
isolation,
dosage,
delivery
methods
Further
is
necessary
elucidate
mechanisms
underlying
sEVs-mediated
regeneration
facilitate
translation
into
therapies.
Extracellular
vesicles
(EVs)
are
cell
derived
nanovesicles
which
implicated
in
both
physiological
and
pathological
intercellular
communication,
including
the
initiation,
progression,
metastasis
of
cancer.
The
exchange
biomolecules
between
stromal
cells
cancer
via
EVs
can
provide
a
window
to
monitor
development
real
time
for
better
diagnostic
interventional
strategies.
In
addition,
process
secretion
internalization
by
tumor
microenvironment
(TME)
be
exploited
delivering
therapeutics.
have
potential
targeted,
biocompatible,
efficient
delivery
platform
treatment
other
diseases.
Natural
as
well
engineered
nanomedicine
immense
disease
intervention.
Here,
we
an
overview
current
knowledge
EVs'
function
therapeutic
applications
setting,
EV
engineering
Bioengineering,
Год журнала:
2022,
Номер
9(3), С. 99 - 99
Опубликована: Фев. 27, 2022
Osteoarthritis
(OA)
is
a
prevalent
and
debilitating
age-related
joint
disease
characterized
by
articular
cartilage
degeneration,
synovial
membrane
inflammation,
osteophyte
formation,
as
well
subchondral
bone
sclerosis.
OA
drugs
at
present
are
mainly
palliative
do
not
halt
or
reverse
progression.
Currently,
no
disease-modifying
(DMOADs)
available
total
arthroplasty
remains
last
resort.
Therefore,
there
an
urgent
need
for
the
development
of
efficacious
treatments
management.
Among
all
novel
pharmaco-therapeutical
options,
exosome-based
therapeutic
strategies
highly
promising.
Exosome
cargoes,
which
include
proteins,
lipids,
cytokines,
various
RNA
subtypes,
potentially
capable
regulating
intercellular
communications
gene
expression
in
target
cells
tissues
involved
development.
With
extensive
research
recent
years,
exosomes
studies
longer
limited
to
classic,
mesenchymal
stem
cell
(MSC)-derived
vesicles.
New
origins,
structures,
functions
constantly
being
discovered
investigated.
This
review
systematically
summarizes
non-classic
biosynthesis,
extraction
exosomes,
describes
modification
delivery
techniques,
explores
their
role
pathogenesis
progression,
discusses
potential
hurdles
overcome
treatment.
npj Regenerative Medicine,
Год журнала:
2022,
Номер
7(1)
Опубликована: Сен. 16, 2022
Osteoarthritis
(OA)
is
the
leading
cause
of
disability
worldwide.
Considerable
progress
has
been
made
using
stem-cell-derived
therapy.
Increasing
evidence
demonstrated
that
therapeutic
effects
BMSCs
in
chondrogenesis
could
be
attributed
to
secreted
small
extracellular
vesicles
(sEVs).
Herein,
we
investigated
feasibility
applying
engineered
EVs
with
chondrogenic
priming
as
a
biomimetic
tool
chondrogenesis.
We
derived
from
TGFβ3-preconditioned
presented
enriched
specific
miRNAs
transferred
native
promote
In
addition,
found
rich
miR-455
promoted
OA
alleviation
and
cartilage
regeneration
by
activating
SOX11/FOXO
signaling
pathway.
Moreover,
designed
T3-EV
hydrogel
showed
great
potential
defect
treatment.
Our
findings
provide
new
means
apply
biosafe
primed-BMSCs
for
repair
treatment,
expanding
understanding
development
modulated
EV-miRNAs
vivo.
Journal of Controlled Release,
Год журнала:
2023,
Номер
361, С. 694 - 716
Опубликована: Авг. 19, 2023
Extracellular
vesicles
(EVs)
are
nanosized
intercellular
messengers
that
bear
enormous
application
potential
as
biological
drug
delivery
vehicles.
Much
progress
has
been
made
for
loading
or
decorating
EVs
with
proteins,
peptides
RNAs
using
genetically
engineered
donor
cells,
but
post-isolation
synthetic
drugs
and
from
natural
sources
remains
challenging.
In
particular,
quantitative
unambiguous
data
assessing
whether
how
small
molecules
associate
versus
other
components
in
the
samples
still
lacking.
Here
we
describe
systematic
characterisation
of
passive
EV
based
on
hydrophobic
interactions
-
either
through
direct
adsorption
compounds,
by
membrane
anchoring
hydrophilic
ligands
via
cholesterol
tags.
As
revealed
single
vesicle
imaging,
both
ligand
types
bind
to
CD63
positive
(exosomes),
however
also
non-specifically
vesicles,
particles,
serum
proteins.
The
compounds
Curcumin
Terbinafine
aggregate
no
apparent
saturation
up
106-107
per
quantified
liquid
chromatography
high
resolution
mass
spectrometry
(LC-HRMS).
For
density
resulted
formation
a
population
large,
electron-dense
detected
cryo-transmission
electron
microscopy
(TEM),
reduced
cell
uptake
toxic
gain
function
Curcumin-EVs.
contrast,
tagging
mdm2-targeted
cyclic
peptide
saturated
at
densities
ca
104-105
vesicle,
lipidomics
showing
addition
to,
rather
than
replacement
endogenous
cholesterol.
Cholesterol
anchored
did
not
change
EVs'
size
morphology,
such
retained
their
activity
without
inducing
toxicity.
However,
cholesterol-anchored
were
rapidly
shed
presence
serum.
Based
these
data,
conclude
(1)
methods
allow
prone
unspecific
compound
binding
redistribution
if
present
sample,
(2)
needs
substantial
optimization
formulation
stability
vivo
applications,
whereas
(3)
careful
titration
is
warranted
when
relying
mitigate
changes
physicochemical
properties,
loss
Pharmaceutics,
Год журнала:
2023,
Номер
15(2), С. 333 - 333
Опубликована: Янв. 19, 2023
Plant-derived
nanovesicles
(PDNVs)
have
become
attractive
alternatives
to
mammalian
cell-derived
extracellular
vesicles
(EVs)
both
as
therapeutic
approaches
and
drug-delivery
vehicles.
In
this
study,
we
isolated
tomato
fruit-derived
NVs
separated
them
by
the
iodixanol
density
gradient
ultracentrifugation
(DGUC)
into
twelve
fractions.
Three
visible
bands
were
observed
at
densities
1.064
±
0.007
g/mL,
1.103
0.006
g/mL
1.122
0.012
g/mL.
Crude
PDNVs
DGUC
fractions
characterized
particle
size-distribution,
concentration,
lipid
protein
contents
well
composition
using
mass
spectrometry-based
proteomics.
Cytotoxicity
anti-inflammatory
activity
of
associated
these
assessed
in
lipopolysaccharide
(LPS)-stimulated
human
monocytic
THP-1
cell
culture.
The
middle
low-density
showed
a
significant
reduction
LPS-induced
inflammatory
IL-1β
cytokine
mRNA
production.
Functional
analysis
proteins
identified
reveals
presence
14-3-3
proteins,
endoplasmic
reticulum
luminal
binding
GTP
gene
ontology
(GO)
term
GO:0050794
regulation
several
cellular
processes
including
inflammation.
most
abundant
middle-density
fraction
was
loaded
with
curcumin
direct
loading,
sonication
extrusion
methods
compared.
highest
entrapment
efficiency
drug
loading
capacity
obtained
loading.
Curcumin
increased
basal
PDNVs.
Medicine in Drug Discovery,
Год журнала:
2023,
Номер
20, С. 100163 - 100163
Опубликована: Окт. 12, 2023
Exosomes
are
vital
for
cellular
communication
and
the
transfer
of
materials
between
cells.
Recently,
it
has
been
shown
that
nanoparticles
may
be
used
as
diagnostic
indicators
a
potential
medicine
delivery
strategy
due
to
their
nanoscale
size
capacity
carry
biological
components
target
have
an
appealing
practical
approach
drug
administration
in
cancer
treatment
biocompatibility,
high
stability,
favoured
tumour
homing,
adjustable
targeting
efficacy.
While
exosomes
properties
make
them
useful
transporting
bioactive
compounds,
gap
our
present
understanding
exosome
biology
most
optimal
condition
use
remains.
The
rationale
using
strategy,
production
purification,
bioengineering
targeting,
uptake
distribution,
efficacy,
immune
response,
other
factors
will
all
discussed
this
study.
Modern
methods
required
observing,
categorising,
sorting
diversity.
Improvements
nanotechnology
knowledge
undoubtedly
speed
up
field's
medical
uses.
Journal of Extracellular Vesicles,
Год журнала:
2024,
Номер
13(7)
Опубликована: Июнь 28, 2024
Abstract
Extracellular
vesicles
have
gained
wide
momentum
as
potential
therapeutics
for
osteoarthritis,
a
highly
prevalent
chronic
disease
that
still
lacks
an
approved
treatment.
The
membrane‐bound
are
secreted
by
all
cells
carrying
different
cargos
can
serve
both
biomarkers
and
modifiers.
Nonetheless,
despite
significant
peak
in
research
regarding
EVs
OA
therapeutics,
clinical
implementation
seems
distant.
In
addition
to
scalability
standardization
challenges,
researchers
often
omit
focus
on
consider
the
proper
tropism
of
vesicles,
practicality
relevance
their
source,
low
native
therapeutic
efficacy,
whether
they
address
whole.
These
considerations
necessary
better
understand
light
been
comprehensively
discussed
ultimately
summarized
this
review
into
conceptualized
framework
termed
nanodiamond
concept.
Future
perspectives
also
discussed,
alternatives
presented
some
challenges
concerns.
