Mosquito E-20-Monooxygenase Gene Knockout Increases Dengue Virus Replication in Aedes aegypti Cells DOI Creative Commons
Bingxue Li, Di Wang, Xiaoxue Xie

и другие.

Viruses, Год журнала: 2024, Номер 16(4), С. 525 - 525

Опубликована: Март 28, 2024

E-20-monooxygenase (E20MO) is an enzymatic product of the shade (shd) locus (cytochrome p450, E20MO). Initially discovered in Drosophila, E20MO facilitates conversion ecdysone (E) into 20-hydroxyecdysone (20E) and crucial for oogenesis. Prior research has implicated 20E growth, development, insecticide resistance. However, little attention been given to association between gene DENV2 infection. The transcriptome Ae. aegypti cells (Aag2 cells) infected with revealed presence gene. subsequent quantification expression levels Aag2 post-DENV infection was carried out. A CRISPR/Cas9 system utilized create knockout cell line (KO), which then subjected DENV Analyses copies KO wild-type (WT) were conducted at different days post-infection (dpi). Plasmids containing constructed transfected cells, pre- post-transfection viral copy comparisons. Gene increased after Subsequently, a successful generation verification code-shifting mutations both DNA RNA achieved. Furthermore, significantly elevated observed mid-infection phase line. Viral lower plasmids E20MO, compared cells. Through plasmid complementation experiments role controlling replication demonstrated. These findings contribute our understanding intricate biological interactions mosquitoes arboviruses.

Язык: Английский

Reactive oxygen species are regulated by immune deficiency and Toll pathways in determining the host specificity of honeybee gut bacteria DOI Creative Commons

Lizhen Guo,

Junbo Tang, Min Tang

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2023, Номер 120(33)

Опубликована: Авг. 9, 2023

Host specificity is observed in gut symbionts of diverse animal lineages. But how hosts maintain while rejecting their close relatives remains elusive. We use eusocial bees and codiversified bacteria to understand host regulation driving symbiotic specificity. The cross-inoculation bumblebee Gilliamella induced higher prostaglandin the honeybee gut, promoting a pronounced response through immune deficiency (IMD) Toll pathways. Gene silencing vitamin C treatments indicate that reactive oxygen species (ROS), not antimicrobial peptides, acts as effector inhibiting non-native strain. Quantitative PCR RNAi further reveal regulatory function IMD pathways, which Relish dorsal-1 may regulate Dual Oxidase ( Duox ) for ROS production. Therefore, maintains by creating hostile environment exotic bacteria, differential its system, reflecting co-opting existing machinery evolved combat pathogens.

Язык: Английский

Процитировано

34
Curing mosquitoes with genetic approaches for malaria control DOI
Mary Kefi, Víctor Cardoso-Jaime, Sally A. Saab

и другие.

Trends in Parasitology, Год журнала: 2024, Номер 40(6), С. 487 - 499

Опубликована: Май 16, 2024

Язык: Английский

Процитировано

10
Ecdysone-controlled nuclear receptor ERR regulates metabolic homeostasis in the disease vector mosquito Aedes aegypti DOI Creative Commons
Dan‐Qian Geng, Xue-Li Wang,

Xiang‐Yang Lyu

и другие.

PLoS Genetics, Год журнала: 2024, Номер 20(3), С. e1011196 - e1011196

Опубликована: Март 11, 2024

Hematophagous mosquitoes require vertebrate blood for their reproductive cycles, making them effective vectors transmitting dangerous human diseases. Thus, high-intensity metabolism is needed to support events of female mosquitoes. However, the regulatory mechanism linking and reproduction in remains largely unclear. In this study, we found that expression estrogen-related receptor (ERR) , a nuclear receptor, activated by direct binding 20-hydroxyecdysone (20E) ecdysone (EcR) response element (EcRE) ERR promoter region during gonadotropic cycle Aedes aegypti (named AaERR). RNA interference (RNAi) AaERR led delayed development ovaries. mRNA abundance genes encoding key enzymes involved carbohydrate (CM)— glucose-6-phosphate isomerase ( GPI ) pyruvate kinase PYK )—was significantly decreased knockdown mosquitoes, while levels metabolites, such as glycogen, glucose, trehalose, were elevated. The fatty acid synthase FAS was notably downregulated, lipid accumulation reduced depletion. Dual luciferase reporter assays electrophoretic mobility shift (EMSA) determined directly metabolic genes, corresponding AaERR-responsive motif these genes. Our results have revealed an important role regulation mosquito offer novel target control.

Язык: Английский

Процитировано

8
Mapping the functional form of the trade-off between infection resistance and reproductive fitness under dysregulated immune signaling DOI Creative Commons
Justin T. Critchlow, Arun Prakash,

Katherine Y. Zhong

и другие.

PLoS Pathogens, Год журнала: 2024, Номер 20(2), С. e1012049 - e1012049

Опубликована: Фев. 26, 2024

Immune responses benefit organismal fitness by clearing parasites but also exact costs associated with immunopathology and energetic investment. Hosts manage these tightly regulating the induction of immune signaling to curtail excessive restore homeostasis. Despite theoretical importance turning off response mitigate costs, experimentally connecting variation in negative regulation remains a frontier evolutionary immunology. In this study, we used dose-response approach manipulate RNAi-mediated knockdown efficiency cactus (IκBα), central regulator Toll pathway signal transduction flour beetles (Tribolium castaneum). By titrating activity across four distinct levels, derived shape relationship between investment traits host fitness, including infection susceptibility, lifespan, fecundity, body mass, gut Cactus knock-down increased overall magnitude inducible delayed their resolution dsRNA dose-dependent manner, promoting survival resistance following bacterial infection. However, benefits were counterbalanced integrity. Our results allowed us move beyond qualitative identification trade-off actually derive its functional form. This paves way quantitatively compare evolution impact regulatory elements on life-history trade-offs filling crucial gap our conceptual models network maintenance natural systems.

Язык: Английский

Процитировано

7
Steroid hormone regulation of innate immunity in Drosophila melanogaster DOI Creative Commons
Scott A. Keith

PLoS Genetics, Год журнала: 2023, Номер 19(6), С. e1010782 - e1010782

Опубликована: Июнь 15, 2023

Endocrine signaling networks control diverse biological processes and life history traits across metazoans. In both invertebrate vertebrate taxa, steroid hormones regulate immune system function in response to intrinsic environmental stimuli, such as microbial infection. The mechanisms of this endocrine-immune regulation are complex constitute an ongoing research endeavor facilitated by genetically tractable animal models. 20-hydroxyecdysone (20E) is the major hormone arthropods, primarily studied for its essential role mediating developmental transitions metamorphosis; 20E also modulates innate immunity a variety insect taxa. This review provides overview our current understanding 20E-mediated responses. prevalence correlations between 20E-driven activation summarized range holometabolous insects. Subsequent discussion focuses on studies conducted using extensive genetic resources available Drosophila that have begun reveal underlying contexts development bacterial Lastly, I propose directions future into will advance knowledge how interactive endocrine coordinate animals' physiological responses microbes.

Язык: Английский

Процитировано

12
mosGILT controls innate immunity and germ cell development in Anopheles gambiae DOI Creative Commons
Gunjan Arora, Xiao‐Tian Tang, Yingjun Cui

и другие.

BMC Genomics, Год журнала: 2024, Номер 25(1)

Опубликована: Янв. 9, 2024

Gene-edited mosquitoes lacking a gamma-interferon-inducible lysosomal thiol reductase-like protein, namely (mosGILT

Язык: Английский

Процитировано

5
Juvenile Hormone as a contributing factor in establishing midgut microbiota for fecundity and fitness enhancement in adult female Aedes aegypti DOI Creative Commons
Mabel L. Taracena, Ana Beatriz Walter‐Nuno, Vanessa Bottino-Rojas

и другие.

Communications Biology, Год журнала: 2024, Номер 7(1)

Опубликована: Июнь 5, 2024

Abstract Understanding the factors influencing mosquitoes’ fecundity and longevity is important for designing better more sustainable vector control strategies, as these parameters can impact their vectorial capacity. Here, we address how mating affects midgut growth in Aedes aegypti , what role Juvenile Hormone (JH) plays this process, it impacts mosquito’s immune response microbiota. Our findings reveal that JH induce growth. Additionally, establishment of a native bacterial population due to JH-dependent suppression has reproductive outcomes. Specific downregulation AMPs with an increase bacteria abundance gut results increased egg counts longer lifespans. Overall, provide evidence cross-talk between response, epithelial tissue, cell cycle regulation, mechanisms governing trade-offs nutrition, immunity, reproduction at cellular level mosquito gut.

Язык: Английский

Процитировано

4
Gut symbiont-derived sphingosine modulates vector competence in Aedes mosquitoes DOI Creative Commons
Xiaomei Sun, Yanhong Wang, Fei Yuan

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Сен. 19, 2024

Язык: Английский

Процитировано

4
Chr23-miR-200s and Dmrt1 Control Sexually Dimorphic Trade-Off Between Reproduction and Growth in Zebrafish DOI Open Access

Si Ge,

Ying Liu, Haoran Huang

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1785 - 1785

Опубликована: Фев. 19, 2025

In animals, a trade-off exists between reproduction and growth, which are the most fundamental traits. Males females exhibit profound differences in growth fish species. However, precise molecular mechanism governing this phenomenon is still not clear. Here, we uncovered that chr23-miR-200s dmrt1 knockout specifically caused an impairment increase body female male zebrafish, respectively. Chr23-miR-200s Dmrt1 directly regulate stat5b gene by targeting its 3'UTR promoter. The loss of completely abolished elevated performance chr23-miR-200s-KO or dmrt1-/- zebrafish. Moreover, transgenic zebrafish had significantly lower length weight than control males, accompanied significant reduction expression liver fish. summary, our study proposes regulatory model elucidating roles controlling sexually dimorphic growth.

Язык: Английский

Процитировано

0
The AaFoxA factor regulates female reproduction through chromatin remodeling in the mosquito vector Aedes aegypti DOI Creative Commons
Cheng Yang, Xuesong Wang,

Yike Ding

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(9)

Опубликована: Фев. 24, 2025

Female mosquitoes are vectors of many devastating human diseases because they require blood feeding to initiate reproduction. Thus, elucidation molecular mechanisms managing female mosquito reproduction is essential. Although the regulation gene expression during gonadotrophic cycle has been studied in detail, how this process controlled at chromatin level remains unclear. Chromatin must be accessible for transcription factors (TFs) governing expression. A specialized class TFs, called pioneer (PFs), binds and remodels closed chromatin, permitting other TFs bind DNA activate Here, we identified a homolog vertebrate PF FoxA Aedes aegypti used CRISPR-Cas9 system generate deficient AaFoxA . We found that ovary development was severely retarded mutant females. Multiomics biology analyses have shown increased histone acetylation decreased methylation H3K27 by controlling accessibility modification enzymes remodelers. bound loci remodelers, changing their modulating temporal patterns. ecdysone receptor (EcR) E74 loci, indicating important role hormonal reproductive events. Further, CUT&RUN ATAC-seq revealed temporarily making it differentially cycle. Hence, study demonstrates modulates dynamics throughout

Язык: Английский

Процитировано

0