Multi‐Material Volumetric Additive Manufacturing of Hydrogels using Gelatin as a Sacrificial Network and 3D Suspension Bath

Advanced Materials, Год журнала: 2024, Номер 36(34)

Опубликована: Янв. 20, 2024

Abstract Volumetric additive manufacturing (VAM) is an emerging layerless method for the rapid processing of reactive resins into 3D structures, where printing much faster (seconds) than other lithography and direct ink writing methods (minutes to hours). As a vial resin rotates in VAM process, patterned light exposure defines object then that has not undergone gelation can be washed away. Despite promise VAM, there are challenges with soft hydrogel materials from non‐viscous precursors, including multi‐material constructs. To address this, sacrificial gelatin used modulate viscosity support cytocompatible macromers based on poly(ethylene glycol) (PEG), hyaluronic acid (HA), polyacrylamide (PA). After printing, removed by washing at elevated temperature. print constructs, gelatin‐containing as shear‐yielding suspension bath (including HA further properties) extruded define processed defined object. Multi‐material constructs methacrylated (MeHA) methacrylamide (GelMA) printed (as proof‐of‐concept) encapsulated mesenchymal stromal cells (MSCs), local properties guide cell spreading behavior culture.

Язык: Английский

---

Volumetric additive manufacturing: A new frontier in layer-less 3D printing

Additive manufacturing, Год журнала: 2024, Номер 84, С. 104094 - 104094

Опубликована: Март 1, 2024

Volumetric Additive Manufacturing (VAM) is an emerging 3D printing technology that operates by fabricating objects from all points within a medium's volume. This technique capable of producing parts without supporting structures and overprinting around existing structures. Notably, the approach VAM utilises to print time efficient compared traditional additive manufacturing methods with times being measured in seconds minutes instead hours. As this there little comparison or synthesis reported so far literature, thus primary objective review address issue providing comprehensive analysis VAM, delving into its applications, challenges it faces, research advancements made area. also investigates how new are investigated on, literature. In order achieve goal structured literature was conducted thoroughly examine current state VAM. found 30 papers, which were used categorise different methods, explore potential various fields, formulate definition for differentiate other technologies. A key finding while offers rapid fabrication capabilities, currently faces several constraints. These include limited availability commercial printers, complex methodologies, restricted range compatible materials, need specialised equipment. Collectively, these factors could serve as barriers broader adoption technology. addition, lack homogeneity parameters investigate report makes difficult compare contrast works against published field progresses, addressing will be essential unlock applications increase one first explicitly focus on entirety offering valuable insights present directions future research.

Язык: Английский

---

Holographic tomographic volumetric additive manufacturing

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 11, 2025

Язык: Английский

---

A Perfusable Multi‐Hydrogel Vasculature On‐Chip Engineered by 2‐Photon 3D Printing and Scaffold Molding to Improve Microfabrication Fidelity in Hydrogels

Advanced Materials Technologies, Год журнала: 2024, Номер 9(4)

Опубликована: Янв. 4, 2024

Abstract Engineering vasculature networks in physiologically relevant hydrogels represents a challenge terms of both fabrication, due to the cell–bioink interactions, as well subsequent hydrogel‐device interfacing. Here, new cell‐friendly fabrication strategy is presented realize perfusable multi‐hydrogel models supporting co‐culture integrated microfluidic chip. The system comprises two different specifically support growth and proliferation cell types selected for vessel model. First, channels are printed gelatin‐based ink by two‐photon polymerization (2PP) inside device. Then, human lung fibroblast‐laden fibrin hydrogel injected surround network. Finally, endothelial cells seeded channels. printing parameters composition optimized reduce swelling ensure stable model that can be perfused with media. Fabricating structure steps ensures no exposed cytotoxic processes, while still obtaining high fidelity printing. In this work, possibility guide invasion through 3D scaffold perfusion 10 days successfully demonstrated on custom‐made system.

Язык: Английский

---

Photocuring 3D Printing of Hydrogels: Techniques, Materials, and Applications in Tissue Engineering and Flexible Devices

Macromolecular Rapid Communications, Год журнала: 2024, Номер 45(7)

Опубликована: Янв. 25, 2024

Photocuring 3D printing of hydrogels, with sophisticated, delicate structures and biocompatibility, attracts significant attention by researchers possesses promising application in the fields tissue engineering flexible devices. After years development, photocuring technologies hydrogel inks make great progress. Herein, techniques including direct ink writing (DIW), stereolithography (SLA), digital light processing (DLP), continuous liquid interface production (CLIP), volumetric additive manufacturing (VAM), two photon polymerization (TPP) are reviewed. Further, raw materials for (photocurable polymers, monomers, photoinitiators, additives) applications devices also At last, current challenges future perspectives hydrogels discussed.

Язык: Английский

---

Light from Afield: Fast, High-Resolution, and Layer-Free Deep Vat 3D Printing

Chemical Reviews, Год журнала: 2024, Номер 124(14), С. 8787 - 8822

Опубликована: Июль 5, 2024

Harnessing light for cross-linking of photoresponsive materials has revolutionized the field 3D printing. A wide variety techniques leveraging broad-spectrum shaping have been introduced as a way to achieve fast and high-resolution printing, with applications ranging from simple prototypes biomimetic engineered tissues regenerative medicine. Conventional light-based printing use material in layer-by-layer fashion produce complex parts. Only recently, new emerged which deploy multidirection, tomographic, light-sheet or filamented image projections deep into volume resin-filled vat photoinitiation cross-linking. These Deep Vat (DVP) approaches alleviate need layer-wise enable unprecedented fabrication speeds (within few seconds) high resolution (>10 μm). Here, we elucidate physics chemistry these processes, their commonalities differences, well emerging biomedical non-biomedical fields. Importantly, highlight limitations, future scope research that will improve scalability applicability DVP engineering medicine applications.

Язык: Английский

---

SonoPrint: Acoustically Assisted Volumetric 3D Printing for Composites

Advanced Materials, Год журнала: 2024, Номер unknown

Опубликована: Июль 25, 2024

Advances in additive manufacturing composites have transformed aerospace, medical devices, tissue engineering, and electronics. A key aspect of enhancing properties 3D-printed objects involves fine-tuning the material by embedding orienting reinforcement within structure. Existing methods for these reinforcements are limited pattern types, alignment, particle characteristics. Acoustics offers a versatile method to control particles independent their size, geometry, charge, enabling intricate formations. However, integrating acoustics into 3D printing has been challenging due scattering acoustic field between polymerized layers unpolymerized resin, resulting unwanted patterns. To address this challenge, SonoPrint, an innovative acoustically assisted volumetric printer is developed that enables simultaneous patterning entire SonoPrint generates mechanically tunable composite geometries particles, such as microscopic glass, metal, polystyrene, fabricated This employs standing wave create targeted motifs-including parallel lines, radial circles, rhombuses, hexagons, polygons-directly photosensitive completing print just few minutes. enhances structural promises advance printing, unlocking applications biohybrid robots, fabrication.

Язык: Английский

---

Lithography-based 3D printing of hydrogels

Nature Reviews Bioengineering, Год журнала: 2024, Номер unknown

Опубликована: Окт. 16, 2024

Язык: Английский

---

Volumetric Additive Manufacturing for Cell Printing: Bridging Industry Adaptation and Regulatory Frontiers

ACS Biomaterials Science & Engineering, Год журнала: 2025, Номер 11(1), С. 156 - 181

Опубликована: Янв. 2, 2025

Volumetric additive manufacturing (VAM) is revolutionizing the field of cell printing by enabling rapid creation complex three-dimensional cellular structures that mimic natural tissues. This paper explores advantages and limitations various VAM techniques, such as holographic lithography, digital light processing, volumetric projection, while addressing their suitability across diverse industrial applications. Despite significant potential VAM, challenges related to regulatory compliance scalability persist, particularly in context bioprinted In India, lack clear guidelines intellectual property protections poses additional hurdles for companies seeking navigate evolving landscape bioprinting. study emphasizes importance collaboration among industry stakeholders, agencies, academic institutions establish tailored frameworks promote innovation ensuring safety efficacy. By bridging gap between technological advancement oversight, can unlock new opportunities regenerative medicine tissue engineering, transforming patient care therapeutic outcomes.

Язык: Английский

---

Integrating Microfluidics, Hydrogels, and 3D Bioprinting for Personalized Vessel-on-a-Chip Platforms

Biomaterials Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Advancement of vascular models from simple 2D culture to complex vessel-on-a-chip platforms through integration microfluidics, biomimetic hydrogels, and 3D bioprinting, enabling controlled investigation thrombosis mechanisms.

Язык: Английский

---