Therapeutic potential of ursolic acid (UA) and their derivatives with nanoformulations to combat nosocomial pathogens

Future Journal of Pharmaceutical Sciences, Год журнала: 2025, Номер 11(1)

Опубликована: Март 11, 2025

Abstract Background Ursolic acid (UA) is a natural pentacyclic triterpene derived from fruit, herbs, and other plants of the terpenoid category. UA has multi-dynamic antimicrobial activity against various pathogens. However, its poor water solubility, low intestinal mucosal absorption, bioavailability restrict clinical application. Nanotechnology can overcome these deficiencies with nanoformulations: nanoemulsion, nanoparticles, nanoemulgels, liposomes, supramolecular gel. derivatives are used as therapeutic agents have immunomodulatory functions. The main body abstract Nanoformulations popularly known promising delivery system for several drugs to increase their efficacy. act on many cellular targets such bacterial cell envelop, efflux pump, enzymatic inhibition, microbial (fungal virus) virulence factors, demonstrating that it be potential biomedicinal agent antibacterial, antifungal, antiviral activity. Various nanoformulations been reported decrease MIC available nosocomial pathogens Staphylococcus aureus, Escherichia coli, Streptococcus pneumoniae, Klebsiella Pseudomonas aeruginosa. Short conclusion This review highlights traditional modern extraction purification techniques. also focuses pharmaceutical uses treat different types infections. Further, UA’s comprehensive molecular mechanism discussed.

Язык: Английский

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Nature-inspired designing of KLR- and KLS-rich antimicrobial peptides: unleashing the antibiofilm potential of RbP12 against MDR S. aureus and P. aeruginosa

Journal of Antimicrobial Chemotherapy, Год журнала: 2025, Номер unknown

Опубликована: Март 24, 2025

Biofilm formation is a mechanism exhibited by bacteria, making them 10-1000 times more resistant than planktonic cells. The aim was to collect the most suitable characteristics from already available antibiofilm peptides and design novel peptide sequences along with these altogether in one sequence. Antibiofilm were collected AMP database (APD3), sequence analysis performed derive features. An artificial approach, modified filtering technology, chosen sequences, their activity predicted machine-learning prediction models. Antibacterial potential of selected (arginine-based 12; RbP12) assessed against Staphylococcus aureus P10 Pseudomonas aeruginosa PA64. A total 34 designed, which 22 arginine based 12 serine based. All designed have properties. RbP12 found inhibit growth S. completely at an MIC 85 mg/L, while percentage inhibition P. PA64 calculated be 32.1%. Significant biofilms observed case both MTT assay showed no significant cytotoxicity 96% cell viability. higher antibacterial compared With viability, usage on human skin totally safe. Based results, develop self-assembled hydrogels for wound healing future work.

Язык: Английский

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Antimicrobial peptides: a promising frontier to combat antibiotic resistant pathogens

Annals of Medicine and Surgery, Год журнала: 2025, Номер 87(4), С. 2118 - 2132

Опубликована: Март 27, 2025

Язык: Английский

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Antimicrobial peptide DvAMP combats carbapenem-resistant Acinetobacter baumannii infection

International Journal of Antimicrobial Agents, Год журнала: 2024, Номер 63(4), С. 107106 - 107106

Опубликована: Фев. 6, 2024

Язык: Английский

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Recent Progress in the Characterization, Synthesis, Delivery Procedures, Treatment Strategies, and Precision of Antimicrobial Peptides

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(14), С. 11864 - 11864

Опубликована: Июль 24, 2023

Infectious diseases are constantly evolving to bypass antibiotics or create resistance against them. There is a piercing alarm for the need improve design of new effective antimicrobial agents such as peptides which less prone and possess high sensitivity. This would guard public health in combating overcoming stubborn pathogens mitigate incurable diseases; however, emergence peptides’ shortcomings ranging from untimely degradation by enzymes difficulty specific targets major bottleneck achieving these objectives. review aimed at highlighting recent progress peptide development area nanotechnology-based delivery, selectivity indices, synthesis characterization, their doping coating, shortfall approaches. will raise awareness prospective therapeutic medical pharmaceutical industries, sensitive treatment utilization. The knowledge this guide future novel allow highly specific, sensitive, accurate initiate regimens patients enable them have accommodating lifestyles.

Язык: Английский

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Neglected Zoonotic Diseases: Advances in the Development of Cell-Penetrating and Antimicrobial Peptides against Leishmaniosis and Chagas Disease

Pathogens, Год журнала: 2023, Номер 12(7), С. 939 - 939

Опубликована: Июль 15, 2023

In 2020, the WHO established road map for neglected tropical diseases 2021–2030, which aims to control and eradicate 20 diseases, including leishmaniosis Chagas disease. addition, since 2015, has been developing a Global Action Plan on Antimicrobial Resistance. this context, achievement of innovative strategies as an alternative replace conventional therapies is first-order socio-sanitary priority, especially regarding endemic zoonoses in poor regions, such those caused by Trypanosoma cruzi Leishmania spp. infections. scenario, it worth highlighting group natural peptide molecules (AMPs CPPs) that are promising improving therapeutic efficacy against these zoonoses, they avoid development toxicity resistance treatments. This review presents novelties their ability cross whole system cell membranes well stimulate host immune defenses or even serve vectors molecules. The efforts biotechnological sector will make possible overcome limitations antimicrobial peptides through encapsulation functionalization methods obtain approval treatments be used clinical programs eradication

Язык: Английский

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Optimizing Antimicrobial Peptide Design: Integration of Cell-Penetrating Peptides, Amyloidogenic Fragments, and Amino Acid Residue Modifications

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(11), С. 6030 - 6030

Опубликована: Май 30, 2024

The escalating threat of multidrug-resistant pathogens necessitates innovative approaches to combat infectious diseases. In this study, we examined peptides R23FS*, V31KS*, and R44KS*, which were engineered include an amyloidogenic fragment sourced from the S1 protein S. aureus, along with one or two cell-penetrating peptide (CPP) components. We assessed antimicrobial efficacy these in a liquid medium against various strains both Gram-positive bacteria, including aureus (209P 129B strains), MRSA (SA 180 ATCC 43300 B. cereus (strain IP 5832), Gram-negative bacteria such as P. aeruginosa (ATCC 28753 2943 strains) E. coli (MG1655 K12 strains). Peptides R44KS* exhibited activity comparable gentamicin meropenem all tested at concentrations ranging 24 48 μM. showed stronger effect cereus. Notably, displayed high compared R23FS* particularly evident lower concentrations, resulting significant inhibition bacterial growth. Furthermore, modified V31KS* demonstrated enhanced inhibitory effects on growth across different their unmodified counterparts V31KS R44KS. These results highlight potential integrating peptides, fragments, amino acid residue modifications advance innovation field thereby increasing effectiveness broad spectrum pathogens.

Язык: Английский

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Cell-Penetrating Peptides in infection and immunization

Microbiological Research, Год журнала: 2024, Номер 290, С. 127963 - 127963

Опубликована: Ноя. 5, 2024

Язык: Английский

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AMP-Coated TiO2 Doped ZnO Nanomaterials Enhanced Antimicrobial Activity and Efficacy in Otitis Media Treatment by Elevating Hydroxyl Radical Levels

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 2995 - 3007

Опубликована: Март 1, 2024

Background: In the past decades, antimicrobial resistance (AMR) has been a major threat to global public health.Long-term, chronic otitis media is becoming more challenging treat, thus novel antibiotic alternative agents are much needed.Methods: ZnO@TiO 2 @AMP (ATZ NPs) were synthesized through solvothermal method and subjected comprehensive characterization.The in vitro vivo antibacterial effect biocompatibility of ATZ NPs evaluated.For mechanism exploration, we utilized Electron Paramagnetic Resonance (EPR) Spectrometer detect analyze hydroxyl radicals produced by NPs.Results: exhibited spherical structure 99.85 nm, drug-loading rate for ZnO was 20.73%, AMP within 41.86%.Notably, Minimum Inhibitory Concentration (MIC) value against Staphylococcus aureus (S. aureus), methicillin-resistant (MRSA), Streptococcus pneumoniae pneumoniae) 10 μg/mL, Bactericidal (MBC) S. aureus, 50 μg/mL.In comparison model group, treatment with significantly reduces inflammatory exudation middle ear cavity, no observable damage tympanic membrane.Both toxicity tests indicating good NPs.Moreover, EPR spectroscopy results highlighted superior ability generate (•OH) compared NPs.Conclusion: remarkable properties both vitro.This innovative application advanced NPs, bringing great promise media.

Язык: Английский

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Non-traditional approaches for control of antibiotic resistance

Expert Opinion on Biological Therapy, Год журнала: 2023, Номер 23(11), С. 1113 - 1135

Опубликована: Ноя. 2, 2023

ABSTRACTIntroduction The drying up of antibiotic pipeline has necessitated the development alternative therapeutic strategies to control problem antimicrobial resistance (AMR) that is expected kill 10-million people annually by 2050. Newer approaches address shortcomings traditional small-molecule antibiotics – lack specificity, evolvability, and susceptibility mutation-based resistance. These 'non-traditional' molecules are biologicals having a complex structure mode(s) action makes them resilient resistance.Areas covered This review aims provide information about non-traditional drug tackle resistance, from pre-antibiotic era latest developments. We have under in clinic with literature sourced reviewed scholarly articles, official company websites involved innovation concerned therapeutics, press releases regulatory bodies, clinical trial databases.Expert opinion Formal introduction therapies general practice can be quick feasible only if supported companion diagnostics used conjunction established therapies. Owing relatively higher costs, therapeutics require more funding as well clarity path. hopeful these issues adequately addressed before AMR develops into pandemic.KEYWORDS: Non-traditionalantimicrobial resistanceantibioticsantibodiespeptidesphagesmicrobiome engineeringvaccines Article highlights Origins reasons for rampant resistance.Approaches/reforms (administrative clinical) this problem.The narrative been presented manner will impart assertiveness aid readers developing mature their own.Literature search containing on findings research trends relating anti-microbial resistance.Information verified private databases scientific literature.Declaration interestThe authors employees AbGenics LifeSciences Pvt. Ltd. no other relevant affiliations or financial involvement any organization entity interest conflict subject matter materials discussed manuscript apart those disclosed.Reviewer disclosuresPeer reviewers relationships disclose.Additional informationFundingThis paper not funded.

Язык: Английский

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