Cell-penetrating peptides stimulate protein transport on the Twin-arginine translocation pathway DOI Creative Commons

Robert T. McNeilage,

Iniyan Ganesan,

Johnathan Keilman

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 9, 2023

Abstract The Tat pathway is essential for photosynthetic protein transport across plant thylakoid membranes and also ubiquitous throughout prokaryotes archaea. unique amongst translocation pathways as it specializes in transporting folded proteins driven by a proton motive force. Mechanistic details of the actual step (s) remain elusive. Here, we show that membrane thinning stimulates and, conversely, strengthening abolishes transport. We draw parallels from mechanism to cell penetrating peptides propose pore could be toroidal shape lined lipids, those formed peptides. Significance Statement Protein significant cellular activity both eukaryotes. operates bacteria, archaea, chloroplasts, mitochondria. Its action has been difficult decipher, but recent evidence suggests does not use conical proteinaceous channel. Instead, suggested translocate through lipid-lined pores set up thinning. This work supports hypothesis showing membrane-thinning cell-penetrating stimulate chloroplasts bacterial plasma membranes, stabilization blocks pathway. believe this most direct date operating

Language: Английский

Therapeutic potential of ursolic acid (UA) and their derivatives with nanoformulations to combat nosocomial pathogens DOI Creative Commons
Umesh Chand, Pramod Kumar Kushawaha

Future Journal of Pharmaceutical Sciences, Journal Year: 2025, Volume and Issue: 11(1)

Published: March 11, 2025

Abstract Background Ursolic acid (UA) is a natural pentacyclic triterpene derived from fruit, herbs, and other plants of the terpenoid category. UA has multi-dynamic antimicrobial activity against various pathogens. However, its poor water solubility, low intestinal mucosal absorption, bioavailability restrict clinical application. Nanotechnology can overcome these deficiencies with nanoformulations: nanoemulsion, nanoparticles, nanoemulgels, liposomes, supramolecular gel. derivatives are used as therapeutic agents have immunomodulatory functions. The main body abstract Nanoformulations popularly known promising delivery system for several drugs to increase their efficacy. act on many cellular targets such bacterial cell envelop, efflux pump, enzymatic inhibition, microbial (fungal virus) virulence factors, demonstrating that it be potential biomedicinal agent antibacterial, antifungal, antiviral activity. Various nanoformulations been reported decrease MIC available nosocomial pathogens Staphylococcus aureus, Escherichia coli, Streptococcus pneumoniae, Klebsiella Pseudomonas aeruginosa. Short conclusion This review highlights traditional modern extraction purification techniques. also focuses pharmaceutical uses treat different types infections. Further, UA’s comprehensive molecular mechanism discussed.

Language: Английский

Citations

0
Nature-inspired designing of KLR- and KLS-rich antimicrobial peptides: unleashing the antibiofilm potential of RbP12 against MDR S. aureus and P. aeruginosa DOI

Zeeshan Yaseen,

S. Aslam,

Sidra Rahmat Ullah

et al.

Journal of Antimicrobial Chemotherapy, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Biofilm formation is a mechanism exhibited by bacteria, making them 10-1000 times more resistant than planktonic cells. The aim was to collect the most suitable characteristics from already available antibiofilm peptides and design novel peptide sequences along with these altogether in one sequence. Antibiofilm were collected AMP database (APD3), sequence analysis performed derive features. An artificial approach, modified filtering technology, chosen sequences, their activity predicted machine-learning prediction models. Antibacterial potential of selected (arginine-based 12; RbP12) assessed against Staphylococcus aureus P10 Pseudomonas aeruginosa PA64. A total 34 designed, which 22 arginine based 12 serine based. All designed have properties. RbP12 found inhibit growth S. completely at an MIC 85 mg/L, while percentage inhibition P. PA64 calculated be 32.1%. Significant biofilms observed case both MTT assay showed no significant cytotoxicity 96% cell viability. higher antibacterial compared With viability, usage on human skin totally safe. Based results, develop self-assembled hydrogels for wound healing future work.

Language: Английский

Citations

0
Antimicrobial peptides: a promising frontier to combat antibiotic resistant pathogens DOI Open Access
Shalini Shriwastav,

Narinder Kaur,

Mahmudul Hassan

et al.

Annals of Medicine and Surgery, Journal Year: 2025, Volume and Issue: 87(4), P. 2118 - 2132

Published: March 27, 2025

Language: Английский

Citations

0
Antimicrobial peptide DvAMP combats carbapenem-resistant Acinetobacter baumannii infection DOI

Longbing Yang,

Yongfei Gao,

Jin Zhang

et al.

International Journal of Antimicrobial Agents, Journal Year: 2024, Volume and Issue: 63(4), P. 107106 - 107106

Published: Feb. 6, 2024

Language: Английский

Citations

3
Recent Progress in the Characterization, Synthesis, Delivery Procedures, Treatment Strategies, and Precision of Antimicrobial Peptides DOI Open Access
Olalekan Olanrewaju Bakare, Arun Gokul, Lee‐Ann Niekerk

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(14), P. 11864 - 11864

Published: July 24, 2023

Infectious diseases are constantly evolving to bypass antibiotics or create resistance against them. There is a piercing alarm for the need improve design of new effective antimicrobial agents such as peptides which less prone and possess high sensitivity. This would guard public health in combating overcoming stubborn pathogens mitigate incurable diseases; however, emergence peptides’ shortcomings ranging from untimely degradation by enzymes difficulty specific targets major bottleneck achieving these objectives. review aimed at highlighting recent progress peptide development area nanotechnology-based delivery, selectivity indices, synthesis characterization, their doping coating, shortfall approaches. will raise awareness prospective therapeutic medical pharmaceutical industries, sensitive treatment utilization. The knowledge this guide future novel allow highly specific, sensitive, accurate initiate regimens patients enable them have accommodating lifestyles.

Language: Английский

Citations

8
Neglected Zoonotic Diseases: Advances in the Development of Cell-Penetrating and Antimicrobial Peptides against Leishmaniosis and Chagas Disease DOI Creative Commons
Sara M. Robledo, Silvia Pérez‐Silanes, Celia Fernández-Rubio

et al.

Pathogens, Journal Year: 2023, Volume and Issue: 12(7), P. 939 - 939

Published: July 15, 2023

In 2020, the WHO established road map for neglected tropical diseases 2021–2030, which aims to control and eradicate 20 diseases, including leishmaniosis Chagas disease. addition, since 2015, has been developing a Global Action Plan on Antimicrobial Resistance. this context, achievement of innovative strategies as an alternative replace conventional therapies is first-order socio-sanitary priority, especially regarding endemic zoonoses in poor regions, such those caused by Trypanosoma cruzi Leishmania spp. infections. scenario, it worth highlighting group natural peptide molecules (AMPs CPPs) that are promising improving therapeutic efficacy against these zoonoses, they avoid development toxicity resistance treatments. This review presents novelties their ability cross whole system cell membranes well stimulate host immune defenses or even serve vectors molecules. The efforts biotechnological sector will make possible overcome limitations antimicrobial peptides through encapsulation functionalization methods obtain approval treatments be used clinical programs eradication

Language: Английский

Citations

7
Optimizing Antimicrobial Peptide Design: Integration of Cell-Penetrating Peptides, Amyloidogenic Fragments, and Amino Acid Residue Modifications DOI Open Access
Sergey V. Kravchenko, Pavel A. Domnin, Sergei Y. Grishin

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 6030 - 6030

Published: May 30, 2024

The escalating threat of multidrug-resistant pathogens necessitates innovative approaches to combat infectious diseases. In this study, we examined peptides R23FS*, V31KS*, and R44KS*, which were engineered include an amyloidogenic fragment sourced from the S1 protein S. aureus, along with one or two cell-penetrating peptide (CPP) components. We assessed antimicrobial efficacy these in a liquid medium against various strains both Gram-positive bacteria, including aureus (209P 129B strains), MRSA (SA 180 ATCC 43300 B. cereus (strain IP 5832), Gram-negative bacteria such as P. aeruginosa (ATCC 28753 2943 strains) E. coli (MG1655 K12 strains). Peptides R44KS* exhibited activity comparable gentamicin meropenem all tested at concentrations ranging 24 48 μM. showed stronger effect cereus. Notably, displayed high compared R23FS* particularly evident lower concentrations, resulting significant inhibition bacterial growth. Furthermore, modified V31KS* demonstrated enhanced inhibitory effects on growth across different their unmodified counterparts V31KS R44KS. These results highlight potential integrating peptides, fragments, amino acid residue modifications advance innovation field thereby increasing effectiveness broad spectrum pathogens.

Language: Английский

Citations

2
Cell-Penetrating Peptides in infection and immunization DOI

Yongliang Du,

Yan Xiong,

Zhou Sha

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 290, P. 127963 - 127963

Published: Nov. 5, 2024

Language: Английский

Citations

2
AMP-Coated TiO2 Doped ZnO Nanomaterials Enhanced Antimicrobial Activity and Efficacy in Otitis Media Treatment by Elevating Hydroxyl Radical Levels DOI Creative Commons

Qianyu Bai,

Yichi Zhang,

Runqiu Cai

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 2995 - 3007

Published: March 1, 2024

Background: In the past decades, antimicrobial resistance (AMR) has been a major threat to global public health.Long-term, chronic otitis media is becoming more challenging treat, thus novel antibiotic alternative agents are much needed.Methods: ZnO@TiO 2 @AMP (ATZ NPs) were synthesized through solvothermal method and subjected comprehensive characterization.The in vitro vivo antibacterial effect biocompatibility of ATZ NPs evaluated.For mechanism exploration, we utilized Electron Paramagnetic Resonance (EPR) Spectrometer detect analyze hydroxyl radicals produced by NPs.Results: exhibited spherical structure 99.85 nm, drug-loading rate for ZnO was 20.73%, AMP within 41.86%.Notably, Minimum Inhibitory Concentration (MIC) value against Staphylococcus aureus (S. aureus), methicillin-resistant (MRSA), Streptococcus pneumoniae pneumoniae) 10 μg/mL, Bactericidal (MBC) S. aureus, 50 μg/mL.In comparison model group, treatment with significantly reduces inflammatory exudation middle ear cavity, no observable damage tympanic membrane.Both toxicity tests indicating good NPs.Moreover, EPR spectroscopy results highlighted superior ability generate (•OH) compared NPs.Conclusion: remarkable properties both vitro.This innovative application advanced NPs, bringing great promise media.

Language: Английский

Citations

2
Non-traditional approaches for control of antibiotic resistance DOI

Siddharth C Umarje,

Sanjiban K. Banerjee

Expert Opinion on Biological Therapy, Journal Year: 2023, Volume and Issue: 23(11), P. 1113 - 1135

Published: Nov. 2, 2023

ABSTRACTIntroduction The drying up of antibiotic pipeline has necessitated the development alternative therapeutic strategies to control problem antimicrobial resistance (AMR) that is expected kill 10-million people annually by 2050. Newer approaches address shortcomings traditional small-molecule antibiotics – lack specificity, evolvability, and susceptibility mutation-based resistance. These 'non-traditional' molecules are biologicals having a complex structure mode(s) action makes them resilient resistance.Areas covered This review aims provide information about non-traditional drug tackle resistance, from pre-antibiotic era latest developments. We have under in clinic with literature sourced reviewed scholarly articles, official company websites involved innovation concerned therapeutics, press releases regulatory bodies, clinical trial databases.Expert opinion Formal introduction therapies general practice can be quick feasible only if supported companion diagnostics used conjunction established therapies. Owing relatively higher costs, therapeutics require more funding as well clarity path. hopeful these issues adequately addressed before AMR develops into pandemic.KEYWORDS: Non-traditionalantimicrobial resistanceantibioticsantibodiespeptidesphagesmicrobiome engineeringvaccines Article highlights Origins reasons for rampant resistance.Approaches/reforms (administrative clinical) this problem.The narrative been presented manner will impart assertiveness aid readers developing mature their own.Literature search containing on findings research trends relating anti-microbial resistance.Information verified private databases scientific literature.Declaration interestThe authors employees AbGenics LifeSciences Pvt. Ltd. no other relevant affiliations or financial involvement any organization entity interest conflict subject matter materials discussed manuscript apart those disclosed.Reviewer disclosuresPeer reviewers relationships disclose.Additional informationFundingThis paper not funded.

Language: Английский

Citations

4