Applied Microbiology and Biotechnology,
Год журнала:
2023,
Номер
107(14), С. 4429 - 4445
Опубликована: Май 29, 2023
Abstract
Stem
cell–based
cell
therapeutics
and
especially
those
based
on
human
mesenchymal
stem
cells
(hMSCs)
induced
pluripotent
(hiPSCs)
are
said
to
have
enormous
developmental
potential
in
the
coming
years.
Their
applications
range
from
treatment
of
orthopedic
disorders
cardiovascular
diseases
autoimmune
even
cancer.
However,
while
more
than
27
hMSC-derived
currently
commercially
available,
hiPSC-based
yet
complete
regulatory
approval
process.
Based
a
review
current
available
therapeutic
products
upcoming
hiPSC-derived
phase
2
3,
this
paper
compares
therapy
manufacturing
process
between
these
two
types.
Moreover,
similarities
as
well
differences
highlighted
resulting
impact
production
discussed.
Here,
emphasis
is
placed
(i)
hMSC
hiPSC
characteristics,
safety,
ethical
aspects,
(ii)
their
morphology
requirements,
(iii)
2-
3-dimensional
cultivations
dependence
applied
culture
medium
mode.
In
doing
so,
also
downstream
processing
aspects
covered
role
single-use
technology
Key
points
•
Mesenchymal
exhibit
distinct
behaviors
during
cultivation
Single-use
stirred
bioreactor
systems
preferred
for
both
types
Future
research
should
adapt
modify
processes
devices
Stem Cell Research & Therapy,
Год журнала:
2022,
Номер
13(1)
Опубликована: Июль 28, 2022
Abstract
Parkinson's
disease
(PD)
is
the
second
most
predominant
neurodegenerative
worldwide.
It
recognized
clinically
by
severe
complications
in
motor
function
caused
progressive
degeneration
of
dopaminergic
neurons
(DAn)
and
dopamine
depletion.
As
current
standard
treatment
focused
on
alleviating
symptoms
through
Levodopa,
developing
neuroprotective
techniques
critical
for
adopting
a
more
pathology-oriented
therapeutic
approach.
Regenerative
cell
therapy
has
provided
us
with
an
unrivalled
platform
evaluating
potentially
effective
novel
methods
treating
illnesses
over
last
two
decades.
Mesenchymal
stem
cells
(MSCs)
are
promising,
as
they
can
differentiate
into
produce
neurotrophic
substances.
The
precise
process
which
repair
neuronal
injury
unknown,
MSC-derived
exosomes
suggested
to
be
responsible
significant
portion
such
effects.
present
review
discusses
application
mesenchymal
PD
treatment.
Stem Cell Reviews and Reports,
Год журнала:
2024,
Номер
20(4), С. 938 - 966
Опубликована: Фев. 26, 2024
The
aim
of
the
study
is
to
determine
effectiveness
stem
cells
in
scaffolds
treatment
bone
deficits,
regard
regeneration,
safety,
rehabilitation
and
quality
life
humans.
systematic
review
was
conducted
accordance
with
PRISMA
2020.
A
search
three
engines
two
registries
lastly
29-9-2022.for
studies
last
15
years.
risk
bias
assessed
RoB-2,
ROBINS-
I
NIH
Quality
Before-After
(Pre-Post)
Studies
no
Control
group.
certainty
results
GRADE
assessment
tool.
Due
heterogeneity,
were
reported
tables,
graphs
narratively.
protocol
published
PROSPERO
registration
number
CRD42022359049.
Of
10,091
retrieved,
14
meeting
inclusion
criteria,
qualitatively
analyzed.
138
patients
treated
mesenchymal
scaffolds,
showing
healing
all
cases,
even
better
than
standard
care.
adverse
events
mild
most
cases
surgery
received.
When
assessed,
there
a
deficit
gain
detected.
Although
heterogeneity
between
small
patients,
administration
seems
safe
effective
regeneration
defects.
These
pave
way
for
conduction
more
clinical
trials,
greater
participants,
standardized
procedures.
Life Sciences,
Год журнала:
2024,
Номер
351, С. 122761 - 122761
Опубликована: Июнь 12, 2024
Mesenchymal
Stromal
Cells
(MSCs)
offer
tremendous
potential
for
the
treatment
of
various
diseases
and
their
healing
properties
have
been
explored
in
hundreds
clinical
trials.
These
trails
primarily
focus
on
immunological
neurological
disorders,
as
well
regenerative
medicine.
Adipose
tissue
is
a
rich
source
mesenchymal
stromal
cells
methods
to
obtain
culture
adipose-derived
MSCs
(AD-MSCs)
established.
Promising
results
from
pre-clinical
testing
AD-MSCs
activity
prompted
trials
that
further
led
approval
complex
perianal
fistulas
Crohn's
disease
subcutaneous
defects.
However,
AD-MSC
heterogeneity
along
with
manufacturing
protocols
or
different
strategies
boost
create
need
standardized
quality
control
procedures
safety
assessment
intended
cell
product.
High-resolution
transcriptomic
recently
gaining
attention,
they
deliver
insight
into
gene
expression
profiles
individual
cells,
helping
deconstruct
cellular
hierarchy
differentiation
trajectories,
understand
cell-cell
interactions
within
tissues.
This
article
presents
comprehensive
overview
completed
evaluating
efficacy
treatment,
together
current
single-cell
studies
human
AD-MSC.
Furthermore,
our
work
emphasizes
increasing
significance
research
elucidating
mechanisms
action
predicting
therapeutic
effects.
Bioactive Materials,
Год журнала:
2024,
Номер
37, С. 153 - 171
Опубликована: Март 21, 2024
Due
to
their
immunomodulatory
function,
mesenchymal
stromal
cells
(MSCs)
are
a
promising
therapeutic
with
the
potential
treat
neuroinflammation
associated
neurodegenerative
diseases.
This
function
is
mediated
by
secreted
extracellular
vesicles
(MSC-EVs).
Despite
established
safety,
MSC
clinical
translation
has
been
unsuccessful
due
inconsistent
outcomes
resulting
from
functional
heterogeneity.
Current
approaches
mitigate
heterogeneity
include
'priming'
MSCs
inflammatory
signals
enhance
function.
However,
comprehensive
evaluation
of
priming
and
its
effects
on
MSC-EV
not
performed.
Furthermore,
therapies
requires
significant
manufacturing
scale-up,
yet
few
studies
have
investigated
in
bioreactors.
As
morphology
shown
predict
we
screened
morphological
response
an
array
evaluated
identity
potency
flasks
We
identified
unique
conditions
corresponding
distinct
morphologies.
These
demonstrated
range
preparation
quality
lipidome,
allowing
us
discover
novel
condition,
as
well
gain
insight
into
mechanisms
microglia
modulation.
Our
screening
approach
application
bioreactor
informs
refinement
larger-scale
enhancement
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Ноя. 29, 2024
The
most
prevalent
and
harmful
injuries
are
burns,
which
still
a
major
global
health
problem.
Burn
can
cause
issues
because
they
boost
the
inflammatory
metabolic
response,
organ
malfunction
systemic
failure.
On
other
hand,
burn
wound
infection
creates
an
environment
that
is
conducive
to
growth
of
bacteria
might
put
patient
at
risk
for
sepsis.
In
addition,
scarring
unavoidable,
this
results
in
patients
having
functional
cosmetic
issues.
Wound
healing
amazing
phenomenon
with
complex
mechanism
deals
different
types
cells
biomolecules.
Cell
therapy
using
stem
one
challenging
treatment
methods
accelerates
wounds.
Since
2000,
use
mesenchymal
(MSCs)
regenerative
medicine
has
increased.
They
be
extracted
from
various
tissues,
such
as
bone
marrow,
fat,
umbilical
cord,
amniotic
membrane.
According
studies,
cell
wounds
increases
angiogenesis,
anti-inflammatory
properties,
slows
progression
fibrosis,
excellent
ability
differentiate
regenerate
damaged
tissue.
Figuring
out
main
preclinical
clinical
problems
stop
people
MSCs
then
suggesting
right
ways
improve
could
help
show
benefits
move
cell-based
forward.
This
review's
objective
was
assess
therapy's
contribution
promotion
healing.
iScience,
Год журнала:
2023,
Номер
26(11), С. 108282 - 108282
Опубликована: Окт. 24, 2023
Extracellular
vesicles
(EVs)
play
a
critical
role
in
various
physiological
and
pathological
processes.
EVs
have
gained
recognition
regenerative
medicine
due
to
their
biocompatibility
low
immunogenicity.
However,
the
practical
application
of
faces
challenges
such
as
limited
targeting
ability,
yield,
inadequate
therapeutic
effects.
To
overcome
these
limitations,
engineered
emerged.
This
review
aims
comprehensively
analyze
engineering
methods
utilized
for
modifying
donor
cells
EVs,
with
focus
on
comparing
potential
between
natural
EVs.
Additionally,
it
investigate
specific
cell
effects
that
crucial
promoting
repair
regeneration,
while
also
exploring
underlying
mechanisms
involved
field
medicine.
International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(9), С. 4693 - 4693
Опубликована: Апрель 23, 2022
The
use
of
mesenchymal
stem
cells
constitutes
a
promising
therapeutic
approach,
as
it
has
shown
beneficial
effects
in
different
pathologies.
Numerous
vitro,
pre-clinical,
and,
to
lesser
extent,
clinical
trials
have
been
published
for
osteoarthritis.
Osteoarthritis
is
type
arthritis
that
affects
diarthritic
joints
which
the
most
common
and
studied
effect
cartilage
degradation.
Nowadays,
known
osteoarthritis
disease
with
very
powerful
inflammatory
component
subchondral
bone
rest
tissues
make
up
joint.
This
may
induce
differentiation
osteoclasts,
bone-resorbing
cells.
Subchondral
degradation
suggested
key
process
pathogenesis
However,
few
studies
directly
focus
on
activity
contrary
what
happens
other
cell
types
joint,
such
chondrocytes,
synoviocytes,
osteoblasts.
In
this
review,
we
try
gather
bibliography
relation
osteoclastogenesis.
Although
find
results,
point
out
need
further
can
support
tool
osteoclasts
their
consequences
osteoarthritic
