Revolutionizing cancer treatment via bioengineered extracellular vesicles: Exploring nanovesicles to fully synthetic solutions DOI Creative Commons

Minmin Shao,

Juliana Rodrigues, Inês Sousa‐Oliveira

и другие.

Applied Materials Today, Год журнала: 2024, Номер 40, С. 102395 - 102395

Опубликована: Авг. 28, 2024

The prevalence of cancer on a global scale has necessitated the development various therapeutic approaches. However, effectiveness and safety existing methods often face some limitations. Nanotechnology emerged as solution, enabling design nanosystems that can effectively deliver drugs. These address challenges such low drug stability solubility, well lack tumor targetability. While nanomaterials offer advantages, certain nanoparticles have their own limitations, which prompted researchers to explore innovative delivery systems. One promising avenue is use extracellular vesicles (EVs), are natural secreted by cells. EVs possess biocompatibility, stability, targeting capabilities, making them ideal candidates for carriers. still remain in this field. include limited production yield, complexity, inefficient cargo loading, controlled release To overcome these challenges, been employing engineering techniques modify structures EVs, enhancing intrinsic properties. Surface modification hybrid systems combine with other shown potential addressing This review focuses landscape crucial role therapeutics diagnosis. It begins exploring biological functions properties EVs. Additionally, introduces strategies isolating shedding light methodologies used harvest minute entities. Finally, highlights biomedical applications bioengineering treatment.

Язык: Английский

Extracellular vesicle-loaded hydrogels for tissue repair and regeneration DOI Creative Commons

Yikun Ju,

Yue Hu, Yang Pu

и другие.

Materials Today Bio, Год журнала: 2022, Номер 18, С. 100522 - 100522

Опубликована: Дек. 21, 2022

Extracellular vesicles (EVs) are a collective term for nanoscale or microscale secreted by cells that play important biological roles. Mesenchymal stem class of with the potential self-healing and multidirectional differentiation. In recent years, numerous studies have shown EVs, especially those mesenchymal cells, can promote repair regeneration various tissues and, thus, significant in regenerative medicine. However, due to rapid clearance capacity circulatory system, EVs barely able act persistently at specific sites target tissues. Hydrogels good biocompatibility loose porous structural properties allow them serve as EV carriers, thereby prolonging retention certain areas slowing release EVs. When needed function sites, EV-loaded hydrogels stand an excellent approach. this review, we first introduce sources, roles, extraction characterization methods describe their current application status. We then review different types discuss factors influencing abilities carry summarize several strategies loading into characterizing hydrogels. Furthermore, applications tissue repair. This article concludes summary state research on outlook future directions, which hope will provide promising ideas researchers.

Язык: Английский

Процитировано

259

Emerging 3D bioprinting applications in plastic surgery DOI Creative Commons
Yang Pu,

Yikun Ju,

Yue Hu

и другие.

Biomaterials Research, Год журнала: 2023, Номер 27(1)

Опубликована: Янв. 3, 2023

Plastic surgery is a discipline that uses surgical methods or tissue transplantation to repair, reconstruct and beautify the defects deformities of human tissues organs. Three-dimensional (3D) bioprinting has gained widespread attention because it enables fine customization implants in patient's area preoperatively while avoiding some adverse reactions complications traditional approaches. In this paper, we review recent research advances application 3D plastic surgery. We first introduce printing process basic principles technology, revealing advantages disadvantages different technologies. Then, describe currently available materials, dissect rationale for special dynamic (4D bioprinting) achieved by varying combination strategy materials. Later, focus on viable clinical applications effects Finally, summarize discuss challenges prospects believe can contribute further development provide lessons related research.

Язык: Английский

Процитировано

67

Exosomes: A potential tool for immunotherapy of ovarian cancer DOI Creative Commons

Xiangjin Gong,

Hao Chi,

Dorothee Franziska Strohmer

и другие.

Frontiers in Immunology, Год журнала: 2023, Номер 13

Опубликована: Янв. 18, 2023

Ovarian cancer is a malignant tumor of the female reproductive system, with very poor prognosis and high mortality rates. Chemotherapy radiotherapy are most common treatments for ovarian cancer, unsatisfactory results. Exosomes subpopulation extracellular vesicles, which have diameter approximately 30–100 nm secreted by many different types cells in various body fluids. highly stable effective carriers immunotherapeutic drugs. Recent studies shown that exosomes involved cellular responses microenvironment, influencing development therapeutic efficacy exhibiting dual roles inhibiting promoting development. also contain variety genes related to immunotherapy could be potential biomarkers diagnosis prognosis. Undoubtedly, great field immunotherapy. However, translation this idea clinic has not occurred. Therefore, it important understand how used regulate progression. In review, we summarize fluids mechanisms influence response. We discuss prospects clinical application exosome-based cancer.

Язык: Английский

Процитировано

55

A ROS/glucose stimulated-responsive ADSCs-derived exosomes-release hydrogel system for diabetic wound healing DOI

Yuanxiang Jing,

Ting Huang, Bin Zhao

и другие.

Chemical Engineering Journal, Год журнала: 2024, Номер 487, С. 150561 - 150561

Опубликована: Март 19, 2024

Язык: Английский

Процитировано

19

The Role of Bacterial Extracellular Vesicles in the Immune Response to Pathogens, and Therapeutic Opportunities DOI Open Access

Eliud S. Peregrino,

Jessica Castañeda‐Casimiro,

Luis Vázquez-Flores

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(11), С. 6210 - 6210

Опубликована: Июнь 5, 2024

Pathogenic bacteria have several mechanisms to evade the host’s immune response and achieve an efficient infection. Bacterial extracellular vesicles (EVs) are a relevant cellular communication mechanism, since they can interact with other bacterial cells host cells. In this review, we focus on EVs produced by some World Health Organization (WHO) priority Gram-negative Gram-positive pathogenic bacteria; spore-producing Mycobacterium tuberculosis (a complex cell wall); Treponema pallidum without lipopolysaccharide). We describe classification general properties of EVs, their role during infections effects response. contain pathogen-associated molecular patterns that activate innate receptors, which leads cytokine production inflammation, but also antigens induce activation B T responses. Understanding many yield new insights pathogenesis clinically important infections, it lead development EV-based diagnostic therapeutic strategies. addition, activators both adaptive responses, constitute promising platform for vaccine development.

Язык: Английский

Процитировано

15

Genetically engineered loaded extracellular vesicles for drug delivery DOI

Zuriñe Erana-Perez,

Manoli Igartúa, Edorta Santos‐Vizcaíno

и другие.

Trends in Pharmacological Sciences, Год журнала: 2024, Номер 45(4), С. 350 - 365

Опубликована: Март 19, 2024

Язык: Английский

Процитировано

12

Bio-nanoparticles loaded with synovial-derived exosomes ameliorate osteoarthritis progression by modifying the oxidative microenvironment DOI Creative Commons

Haifei Cao,

Wan-Xin Li, Hao Zhang

и другие.

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Май 20, 2024

Abstract Background and aims Osteoarthritis (OA) is a prevalent degenerative joint disorder, marked by the progressive degeneration of cartilage, synovial inflammation, subchondral bone hyperplasia. The tissue plays pivotal role in cartilage regulation. Exosomes (EXOs), small membrane-bound vesicles released cells into extracellular space, are crucial mediating intercellular communication facilitating exchange information between tissues. Our study aimed to devise hydrogel microsphere infused with SOD3-enriched exosomes (S-EXOs) protect introduce novel, effective approach for OA treatment. Materials methods We analyzed single-cell sequencing data from 4247 obtained GEO database. Techniques such as PCR, Western Blot, immunofluorescence (IF), assays measure oxidative stress levels were employed validate cartilage-protective properties identified key protein, SOD3. In vivo, mice received intra-articular injections S-EXOs bearing microspheres, effectiveness was assessed using safranine O (S.O) staining IF. Results Single-cell analysis suggested that synovium influences via exocrine release findings revealed purified enhanced antioxidant capacity chondrocytes, maintained matrix metabolism stability. S-EXO group showed significant reduction mitoROS ROS 164.2% ( P < 0.0001) 142.7% 0.0001), respectively, compared IL-1β group. Furthermore, exhibited increased COL II ACAN levels, increments 2.1-fold 3.1-fold over Additionally, decrease MMP13 ADAMTS5 protein expression 42.3% 44.4% respectively. It found S-EXO-containing microspheres could effectively deliver SOD3 significantly mitigate progression. OARSI score markedly decreased Conclusion demonstrated secreted fibroblasts exert protective effect on laden offer promising therapeutic alternative

Язык: Английский

Процитировано

10

Venoms and Extracellular Vesicles: A New Frontier in Venom Biology DOI Creative Commons
Auwal A. Bala,

Naoual Oukkache,

Elda E. Sánchez

и другие.

Toxins, Год журнала: 2025, Номер 17(1), С. 36 - 36

Опубликована: Янв. 14, 2025

Extracellular vesicles (EVs) are nanoparticle-sized secreted by nearly all cell types under normal physiological conditions. In toxicological research, EVs have emerged as a crucial link between public health and multi-omics approaches, offering insights into cellular responses to disease-causing injury agents such environmental biological toxins, contaminants, drugs. Notably, present unique opportunity deepen our understanding of the pathophysiology envenomation natural toxins. Recent advancements in isolating purifying EV cargo, mass spectrometry techniques, bioinformatics positioned potential biomarkers that could elucidate signaling pathways provide valuable information on relationship venomous their mechanisms action, effectiveness antivenoms. Additionally, hold promise proxies for various aspects envenomation, including toxin dosage, characterization, progression, prognosis during therapeutic interventions. These can be explored through technology applied contents from plasma, saliva, or urine samples envenomated individuals, comprehensive integrative approach managing cases.

Язык: Английский

Процитировано

1

Scalable production of anti-inflammatory exosomes from three-dimensional cultures of canine adipose-derived mesenchymal stem cells: production, stability, bioactivity, and safety assessment DOI Creative Commons

Anatcha Thongsit,

Saranyou Oontawee, Parkpoom Siriarchavatana

и другие.

BMC Veterinary Research, Год журнала: 2025, Номер 21(1)

Опубликована: Фев. 20, 2025

The therapeutic potential of exosomes derived from mesenchymal stem cells (MSCs) is increasingly recognized in veterinary medicine. This study explored the feasibility a microcarrier-based three-dimensional (3D) culture system for producing (cEXO). Investigations were conducted to enhance production efficiency, ensure stability, and evaluate cEXO anti-inflammatory applications while assessing their safety profile. 3D improved efficient cEXO, yielding with acceptable profiles, including size approximately 81.22 nm, negative surface charge, high particle concentration (1.32 × 109 particles/mL). Confocal imaging proved dynamic changes cell viability across phases, highlighting challenges maintaining during repeated exosome collection cycles. Characterization via transmission electron microscopy, nanoparticle tracking analysis, zeta-potential measurements confirmed stability functionality particularly when stored at -20 °C. Functional assays showed that exerted significant activity RAW264.7 macrophages an inverse dose-dependent manner, no observed cytotoxicity fibroblasts or macrophages. Acute toxicity testing rats revealed adverse effects on clinical parameters, organ health, body weight, supporting use. highlights scalable robust activity, profiles. These findings advance development cEXO-based therapies support application regenerative

Язык: Английский

Процитировано

1

The developing role of extracellular vesicles in autoimmune diseases: special attention to mesenchymal stem cell-derived extracellular vesicles DOI
Geovanny Genaro Reiván Ortiz,

Neelam Hazoor Zaidi,

Ravinder Saini

и другие.

International Immunopharmacology, Год журнала: 2023, Номер 122, С. 110531 - 110531

Опубликована: Июль 10, 2023

Язык: Английский

Процитировано

20