Urinary
tract
infections
(UTIs)
caused
by
Klebsiella
pneumoniae
can
lead
to
severe
clinical
complications
and
even
death.
An
alternative
treatment
option
for
infected
patients
is
using
bacteriophages
(phages).
In
the
present
study,
we
isolated
phage
KP-1
from
sewage
water
K.
as
a
host.
Whole
genome
analysis
indicated
that
was
double-stranded
linear
176,096-bp
long
DNA
molecule
with
41.8%
GC
content
did
not
contain
virulence
or
antibiotic
resistance
genes.
The
inactivation
potential
of
assessed
in
broth
at
an
MOI
1
10,
maximum
4.9
5.4
log
CFU/mL
observed
after
9
h,
respectively.
10
efficacy
also
urine
evaluate
phage’s
performance
acidic
environment.
A
3.8
h.
results
indicate
could
be
used
control
UTIs
pneumoniae;
however,
further
studies
cocktails
broader
spectrum
activity
phages
micro-
nanocarriers
avoid
negative
effect
low
pH
on
viability,
are
necessary.
Frontiers in Microbiology,
Год журнала:
2025,
Номер
15
Опубликована: Янв. 9, 2025
Klebsiella
pneumoniae
is
a
significant
nosocomial
pathogen
that
has
developed
resistance
to
multiple
antibiotics,
often
forming
biofilms
enhance
its
virulence.
This
study
investigated
the
efficacy
of
novel
nanoformulation,
AgNPs@chitosan-NaF,
in
combating
K.
biofilms.
Antimicrobial
susceptibility
testing
was
performed
assess
antibiotic
profile
isolates.
The
antibiofilm
activity
AgNPs@chitosan-NaF
evaluated
using
crystal
violet
staining
and
scanning
electron
microscopy.
underlying
mechanisms
action
were
through
gene
expression
analysis.
majority
isolates
exhibited
high
levels
multidrug
resistance.
demonstrated
superior
biofilm
inhibition
compared
AgNPs@chitosan,
significantly
reducing
biomass
disrupting
architecture
at
MICs
ranging
from
0.125
1
μg/mL.
Mechanistic
studies
revealed
nanoformulation
downregulated
key
biofilm-associated
genes,
including
treC,
fimA,
mrkA,
ecpA.
While
concentration-dependent
cytotoxic
effect
on
both
normal
cancer
cell
lines,
minimal
cytotoxicity
observed
concentrations
below
31.25
highlights
synergistic
silver
nanoparticles,
chitosan,
sodium
fluoride
emerges
as
promising
therapeutic
strategy
address
challenge
multidrug-resistant
bacterial
infections.
Microorganisms,
Год журнала:
2023,
Номер
11(3), С. 704 - 704
Опубликована: Март 9, 2023
Hypervirulent
Klebsiella
pneumoniae
(hvKp)
is
emerging
worldwide.
Hypermucoviscousity
the
characteristic
trait
that
distinguishes
it
from
classic
K.
(cKp),
which
enables
Kp
to
cause
severe
invasive
infections.
This
research
aimed
investigate
hypermucoviscous
(hmvKp)
phenotype
among
gut
commensal
isolated
healthy
individuals
and
attempted
characterize
genes
encoding
virulence
factors
may
regulate
hypermucoviscosity
trait.
Using
string
test,
50
identified
isolates
individuals’
stool
samples
were
examined
for
investigated
by
transmission
electron
microscopy
(TEM).
Antimicrobial
susceptibility
profiles
of
determined
using
Kirby
Bauer
disc
method.
tested
different
PCR.
Biofilm
formation
was
assayed
microtiter
plate
All
multidrug-resistant
(MDR).
Phenotypically,
42%
hmvKp.
PCR-based
genotypic
testing
revealed
hmvKp
belonged
capsular
serotype
K2.
study
harbored
more
than
one
gene.
The
magA
rmpA
not
detected,
while
terW
gene
present
in
all
isolates.
siderophores
entB
irp2
most
prevalent
(90.5%)
non-hmvKp
(96.6%),
respectively.
wabG
uge
with
rates
90.5%
85.7%,
outcomes
this
highlight
potential
health
risk
diseases,
owing
being
MDR,
harboring
multiple
genes.
absence
essential
related
such
as
phenotypes
suggests
multifactorial
complexity
or
hypervirulence
traits.
Thus,
further
studies
are
warranted
verify
hypermucoviscosity-related
pathogenic
colonization
niches.
Journal of Medicinal Chemistry,
Год журнала:
2023,
Номер
66(16), С. 11555 - 11572
Опубликована: Авг. 11, 2023
Antimicrobial
cationic
peptides
are
intriguing
and
propitious
antibiotics
for
the
future,
even
against
multidrug-resistant
superbugs.
Venoms
serve
as
a
source
of
cutting-edge
therapeutics
innovative,
unexplored
medicines.
In
this
study,
novel
peptide
library
consisting
seven
sequences
was
designed
synthesized
from
snake
venom
cathelicidin,
batroxicidin
(BatxC),
with
inclusion
FLPII
motif
at
N-terminus.
SP1V3_1
demonstrated
exceptional
antibacterial
effectiveness
Escherichia
coli,
methicillin-resistant
Staphylococcus
aureus
(MRSA),
Pseudomonas
aeruginosa,
Klebsiella
pneumoniae
destroyed
bacteria
by
depolarizing,
rupturing,
permeabilizing
their
membranes,
evident
fluorescence
assays,
atomic
force
microscopy,
scanning
electron
microscopy.
observed
to
modulate
immune
response
in
LPS-elicited
U937
cells
exhibited
good
antibiofilm
activity
MRSA
K.
pneumoniae.
The
promoted
wound
healing
disinfection
murine
model.
study
that
is
an
exciting
lead
may
be
explored
further
development
better
therapeutic
peptides.
Antibiotics,
Год журнала:
2024,
Номер
13(2), С. 195 - 195
Опубликована: Фев. 19, 2024
Urinary
tract
infections
(UTIs)
caused
by
resistant
Klebsiella
pneumoniae
can
lead
to
severe
clinical
complications
and
even
death.
An
alternative
treatment
option
for
infected
patients
is
using
bacteriophages.
In
the
present
study,
we
isolated
phage
VB_KPM_KP1LMA
(KP1LMA)
from
sewage
water
a
K.
strain
as
host.
Whole-genome
analysis
indicated
that
genome
was
double-stranded
linear
176,096-bp
long
DNA
molecule
with
41.8%
GC
content
did
not
contain
virulence
or
antibiotic
resistance
genes.
The
inactivation
potential
of
KP1LMA
assessed
in
broth
at
an
MOI
1
10,
maximum
4.9
5.4
log
CFU/mL,
respectively,
observed
after
9
h.
efficacy
10
also
urine
evaluate
phage’s
performance
acidic
environment.
A
3.8
CFU/mL
results
suggest
could
potentially
control
UTI
this
pneumoniae,
indicating
same
procedure
be
used
UTIs
other
strains
if
new
specific
phages
are
isolated.
Although
has
narrow
host
range,
future,
efforts
made
expand
its
spectrum
activity
combine
others,
enabling
use
against
involved
UTIs.
ACS Applied Bio Materials,
Год журнала:
2024,
Номер
7(5), С. 2604 - 2619
Опубликована: Апрель 16, 2024
Biofilms
are
an
intricate
community
of
microbes
that
colonize
solid
surfaces,
communicating
via
a
quorum-sensing
mechanism.
These
microbial
aggregates
secrete
exopolysaccharides
facilitating
adhesion
and
conferring
resistance
to
drugs
antimicrobial
agents.
The
escalating
global
concern
over
biofilm-related
infections
on
medical
devices
underscores
the
severe
threat
human
health.
Carbon
dots
(CDs)
have
emerged
as
promising
substrate
combat
disrupt
biofilm
matrices.
Their
numerous
advantages
such
facile
surface
functionalization
specific
properties,
position
them
innovative
anti-biofilm
Due
their
minuscule
size,
CDs
can
penetrate
cells,
inhibiting
growth
cytoplasmic
leakage,
reactive
oxygen
species
(ROS)
generation,
genetic
material
fragmentation.
Research
has
demonstrated
efficacy
in
biofilms
formed
by
key
pathogenic
bacteria
Microorganisms,
Год журнала:
2024,
Номер
12(4), С. 704 - 704
Опубликована: Март 30, 2024
Pathogenic
biofilms
provide
a
naturally
favorable
barrier
for
microbial
growth
and
are
closely
related
to
the
virulence
of
pathogens.
Postbiotics
from
lactic
acid
bacteria
(LAB)
secondary
metabolites
cellular
components
obtained
by
inactivation
fermentation
broth;
they
have
certain
inhibitory
effect
on
all
stages
pathogen
biofilms.
LAB
drawn
attention
because
their
high
stability,
safety
dose
parameters,
long
storage
period,
which
give
them
broad
application
prospect
in
fields
food
medicine.
The
mechanisms
eliminating
via
postbiotics
mainly
affect
surface
adhesion,
self-aggregation,
virulence,
QS
pathogens
influencing
interspecific
intraspecific
communication.
However,
there
some
factors
(preparation
process
lack
target)
can
limit
antibiofilm
impact
postbiotics.
Therefore,
using
delivery
carrier
optimizing
interfering
be
eliminated.
This
review
summarizes
concept
characteristics
LAB,
focusing
preparation
technology
effect,
applications
limitations
processing
clinical
treatment
also
discussed.
